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Optimal timing of blastocyst vitrification after trophectoderm biopsy for preimplantation genetic screening

trail protocol-translation. (DOC) Acknowledgments We thank Chien-Hong Chen (Lee Women's Hospital) for identifying vitrification and TE biopsy. Author Contributions Conceptualization: Hsiu-Hui Chen ... , Chun-Chia Huang, Tsung-Hsien Lee. Data curation: Hsiu-Hui Chen, Lee-Feng Chien. Formal analysis: Hsiu-Hui Chen. Funding acquisition: Maw-Sheng Lee. Methodology: Hsiu-Hui Chen, Chun-Chia Huang, En-Hui

Magnetic-activated cell sorting for sperm preparation reduces spermatozoa with apoptotic markers and improves the acrosome reaction in couples with unexplained infertility†

BACKGROUND Couples with unexplained infertility (UI) tend to have low fertilization rates with current IVF procedures. Here, we attempted to identify spermatozoa with apoptotic markers in couples with UI and unsuccessful intrauterine insemination (IUI) and we investigated the efficiency and benefit of magnetic-activated cell sorting (MACS) for sperm preparation in such patients...

Successful pregnancy following blastocyst cryopreservation using super-cooling ultra-rapid vitrification

BACKGROUND: Blastocysts were cryopreserved by a new two-step ultra-rapid cooling in super-cooled liquid nitrogen (−205°C). METHODS: There were 308 mouse blastocysts collected from fertile B6CBF1 mice and 249 human blastocysts collected from 51 couples treated with IVF. The blastocysts were super-cooled by a Vit-Master™ and cryoloops after treatment in 50 and 100% vitrification...