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Search: authors:"Hyun-Sook Lee"

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A novel family VIII carboxylesterase hydrolysing third- and fourth-generation cephalosporins

A metagenomic library was constructed from a soil sample of spindle tree-rhizosphere. From this library, one clone with esterase activity was selected. The sequence analysis revealed an open reading frame (EstSTR1) encoded protein of 390 amino acids. EstSTR1 is a family VIII carboxylesterase and retains the S-X-X-K motif conserved in both family VIII carboxylesterases and class C ...