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Search: authors:"James C. du Preez"

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Gene regulation in Kluyveromyces marxianus in the context of chromosomes

statistical analyses. Author Contributions Conceptualization: Du Toit W. P. Schabort, James C. du Preez. Data curation: Du Toit W. P. Schabort. Formal analysis: Du Toit W. P. Schabort. Funding acquisition ... . Software: Du Toit W. P. Schabort. Supervision: James C. du Preez. 14 / 16 Visualization: Du Toit W. P. Schabort. Writing ± original draft: Du Toit W. P. Schabort. Writing ± review & editing: Stephanus G

Elucidation of new condition-dependent roles for fructose-1,6-bisphosphatase linked to cofactor balances

The cofactor balances in metabolism is of paramount importance in the design of a metabolic engineering strategy and understanding the regulation of metabolism in general. ATP, NAD+ and NADP+ balances are central players linking the various fluxes in central metabolism as well as biomass formation. NADP+ is especially important in the metabolic engineering of yeasts for xylose...

Differential RNA-seq, Multi-Network Analysis and Metabolic Regulation Analysis of Kluyveromyces marxianus Reveals a Compartmentalised Response to Xylose

We investigated the transcriptomic response of a new strain of the yeast Kluyveromyces marxianus, in glucose and xylose media using RNA-seq. The data were explored in a number of innovative ways using a variety of networks types, pathway maps, enrichment statistics, reporter metabolites and a flux simulation model, revealing different aspects of the genome-scale response in an...

Opuntia ficus-indica cladodes as feedstock for ethanol production by Kluyveromyces marxianus and Saccharomyces cerevisiae

The feasibility of ethanol production using an enzymatic hydrolysate of pretreated cladodes of Opuntia ficus-indica (prickly pear cactus) as carbohydrate feedstock was investigated, including a comprehensive chemical analysis of the cladode biomass and the effects of limited aeration on the fermentation profiles and sugar utilization. The low xylose and negligible mannose content...