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Elafin is downregulated during breast and ovarian tumorigenesis but its residual expression predicts recurrence

Introduction Elafin is an endogenous serine protease inhibitor. The majority of breast cancer cell lines lack elafin expression compared to human mammary epithelial cells. In this study, we hypothesized that elafin is downregulated during breast and ovarian tumorigenesis. Methods We examined elafin expression by immunohistochemistry (IHC) in specimens of normal breast tissue (n = ...

Selective Detection of Peptide-Oligonucleotide Heteroconjugates Utilizing Capillary HPLC-ICPMS

A method for the selective detection and quantification of peptide:oligonucleotide heteroconjugates, such as those generated by protein:nucleic acid cross-links, using capillary reversed-phase high performance liquid chromatography (cap-RPHPLC) coupled with inductively coupled plasma mass spectrometry detection (ICPMS) is described. The selective detection of phosphorus as 31P+, ...

A Transgenic Drosophila Model for Arsenic Methylation Suggests a Metabolic Rationale for Differential Dose-Dependent Toxicity Endpoints

The mechanisms by which exposure to arsenic induces its myriad pathological effects are undoubtedly complex, while individual susceptibility to their type and severity is likely to be strongly influenced by genetic factors. Human metabolism of arsenic into methylated derivatives, once presumed to result in detoxification, may actually produce species with significantly greater ...

Elucidating the selenium and arsenic metabolic pathways following exposure to the non-hyperaccumulating Chlorophytum comosum, spider plant

Catron 0 Joseph A. Caruso 0 0 Department of Chemistry, University of Cincinnati/Agilent Technologies Metallomics Center of the Americas, University of Cincinnati , Cincinnati, OH 45221-0172 , USA Although

Metallomic Analysis of Macrophages Infected with Histoplasma capsulatum Reveals a Fundamental Role for Zinc in Host Defenses

The fungal pathogen Histoplasma capsulatum evades the innate and adaptive immune responses and thrives within resting macrophages. Cytokines that induce antimicrobial activity, such as granulocyte macrophage colony-stimulating factor (GM-CSF), inhibit H. capsulatum growth in macrophages. Conversely, interleukin 4 inhibits the killing of intracellular pathogens. Using inductively ...

Deconvolution of isobaric interferences in mass spectra

The concept of isobar deconvolution using the mass domain and signal intensity based domains is described. The intensity domain-based approach employs the reconstruction of the observed isotope pattern from the isolated patterns of the isobaric species. The quantitative information is adjusted with the use of the least squares algorithm. The mass domain-based approach employs ...

Interpretation of alkyl diselenide and selenosulfenate mass spectra

The mass spectral fragmentation of aliphatic diselenides and selenosulfenates is analyzed to gain a better understanding of the behavior of these species. The main fragmentation pathways of these species include the fragmentation along the Se-C bond, fragmentation along the Se-Se or Se-S bonds and intra-molecular rearrangements. In general, negative ionization favors the ...

Regulated expression of matrix metalloproteinases, inflammatory mediators, and endometrial matrix remodeling by 17beta-estradiol in the immature rat uterus

Background Administration of a single physiological dose of 17beta-estradiol (E2:40 microg/kg) to the ovariectomized immature rat rapidly induces uterine growth and remodeling. The response is characterized by changes in endometrial stromal architecture during an inflammatory-like response that likely involves activated matrix-metalloproteinases (MMPs). While estrogen is known as ...