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333 papers found.
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(-)-Phenserine Attenuates Soman-Induced Neuropathology

Organophosphorus (OP) nerve agents are deadly chemical weapons that pose an alarming threat to military and civilian populations. The irreversible inhibition of the critical cholinergic degradative enzyme acetylcholinesterase (AChE) by OP nerve agents leads to cholinergic crisis. Resulting excessive synaptic acetylcholine levels leads to status epilepticus that, in turn, results...

24h Urinary Protein Levels and Urine Protein/Creatinine Ratios Could Probably Forecast the Pathological Classification of HSPN

This study aimed to assess the relevance of laboratory tests in Henoch-Schönlein purpura nephritis (HSPN) classification, and determine accurate classification factors. This prospective study included 694 HSPN patients who underwent ultrasound-guided percutaneous renal biopsy (PRB). Renal specimens were scored according to International Study of Kidney Disease in Children (ISKDC...

A CRISPR/Cas9 toolkit for multiplex genome editing in plants

Hui-Li Xing 0 Li Dong 0 Zhi-Ping Wang Hai-Yan Zhang Chun-Yan Han Bing Liu Xue-Chen Wang Qi-Jun Chen 0 Equal contributors State Key Laboratory of Plant Physiology and Biochemistry, College of

A Fragmentation Study on Four Unusual Secoiridoid Trimers, Swerilactones H–K, by Electrospray Tandem Mass Spectrometry

Swerilactones H–K (1–4) as four unprecedented secoiridoid trimers represent a new type of natural product, which has attracted much interest of natural chemists due to their novel skeletons and promising bioactivity. In order to well understand their MS fragmentation behaviors, they were investigated by electrospray ionization ion-trap time-of-flight multistage product ion mass...

A Gain-of-Function Mutation in Tnni2 Impeded Bone Development through Increasing Hif3a Expression in DA2B Mice

Distal arthrogryposis type 2B (DA2B) is an important genetic disorder in humans. However, the mechanisms governing this disease are not clearly understood. In this study, we generated knock-in mice carrying a DA2B mutation (K175del) in troponin I type 2 (skeletal, fast) (TNNI2), which encodes a fast-twitch skeletal muscle protein. Tnni2K175del mice (referred to as DA2B mice...

A Hsp40 Chaperone Protein Interacts with and Modulates the Cellular Distribution of the Primase Protein of Human Cytomegalovirus

Genomic DNA replication is a universal and essential process for all herpesvirus including human cytomegalovirus (HCMV). HCMV UL70 protein, which is believed to encode the primase activity of the viral DNA replication machinery and is highly conserved among herpesviruses, needs to be localized in the nucleus, the site of viral DNA synthesis. No host factors that facilitate the...

A Mixture Model for Robust Point Matching under Multi-Layer Motion

This paper proposes an efficient mixture model for establishing robust point correspondences between two sets of points under multi-layer motion. Our algorithm starts by creating a set of putative correspondences which can contain a number of false correspondences, or outliers, in addition to the true correspondences (inliers). Next we solve for correspondence by interpolating a...

A Neural Network Model for K(λ) Retrieval and Application to Global Kpar Monitoring

Accurate estimation of diffuse attenuation coefficients in the visible wavelengths Kd(λ) from remotely sensed data is particularly challenging in global oceanic and coastal waters. The objectives of the present study are to evaluate the applicability of a semi-analytical Kd(λ) retrieval model (SAKM) and Jamet’s neural network model (JNNM), and then develop a new neural network Kd...

A New Molecular Phylogeny and a New Genus, Pendulorchis, of the Aerides–Vanda Alliance (Orchidaceae: Epidendroideae)

Alliance (Orchidaceae: Epidendroideae) Guo-Qiang Zhang Ke-Wei Liu Li-Jun Chen Xin-Ju Xiao Jun-Wen Zhai Li-Qiang Li Jing Cai Yu-Yun Hsiao Wen-Hui Rao Jie Huang Xue-Yong Ma Shih-Wen Chung Lai- Qiang Huang Wen

A New Myco-Heterotrophic Genus, Yunorchis, and the Molecular Phylogenetic Relationships of the Tribe Calypsoeae (Epidendroideae, Orchidaceae) Inferred from Plastid and Nuclear DNA Sequences

We identified a new holomycotrophic orchid that is related to the myco-heterotrophic Calypsoeae. Because chloroplast genes are primarily lacking or are highly divergent, key morphological characters are either reduced or lost from many myco-heterotrophs, and the phylogenetic relationships of weakly supported paraphyletic Calypsoeae within Epidendroideae have been poorly...

A New Orchid Genus, Danxiaorchis, and Phylogenetic Analysis of the Tribe Calypsoeae

-Wen Zhai Guo-Qiang Zhang Li-Jun Chen Xin-Ju Xiao Ke-Wei Liu Wen-Chieh Tsai Yu- Yun Hsiao Huai-Zhen Tian Jia-Qiang Zhu Mei-Na Wang Fa-Guo Wang Fu-Wu Xing Zhong- Ji n Liu Maria Anisimova, Swiss Federal

A Novel Injectable Calcium Phosphate Cement-Bioactive Glass Composite for Bone Regeneration

Background Calcium phosphate cement (CPC) can be molded or injected to form a scaffold in situ, which intimately conforms to complex bone defects. Bioactive glass (BG) is known for its unique ability to bond to living bone and promote bone growth. However, it was not until recently that literature was available regarding CPC-BG applied as an injectable graft. In this paper, we...

A Picea abies Linkage Map Based on SNP Markers Identifies QTLs for Four Aspects of Resistance to Heterobasidion parviporum Infection

A consensus linkage map of Picea abies, an economically important conifer, was constructed based on the segregation of 686 SNP markers in a F1 progeny population consisting of 247 individuals. The total length of 1889.2 cM covered 96.5% of the estimated genome length and comprised 12 large linkage groups, corresponding to the number of haploid P. abies chromosomes. The sizes of...

A large quantity of novel human antisense transcripts detected by LongSAGE

Motivation: Taking advantage of the high sensitivity and specificity of LongSAGE tag for transcript detection and genome mapping, we analyzed the 632 813 unique human LongSAGE tags deposited in public databases to identify novel human antisense transcripts. Results: Our study identified 45 321 tags that match the antisense strand of 9804 known mRNA sequences, 6606 of which...

A naturally occurring 4-bp deletion in the intron 4 of p53 creates a spectrum of novel p53 isoforms with anti-apoptosis function

p53 functions as a tumor suppressor by transcriptionally regulating the expression of genes involved in controlling cell proliferation or apoptosis. p53 and its isoform Δ133p53/Δ113p53 form a negative regulation loop in that p53 activates the expression of Δ133p53/Δ113p53 while Δ133p53/Δ113p53 specifically antagonizes p53 apoptotic activity. This pathway is especially important...

A new non-enzymatic method for isolating human intervertebral disc cells preserves the phenotype of nucleus pulposus cells

Cells isolated from intervertebral disc (IVD) tissues of human surgical samples are one of potential sources for the IVD cellular therapy. The purpose of this study was to develop a new non-enzymatic method, “tissue incubation”, for isolating human IVD cells. The IVD tissues of annulus fibrosus (AF) and nucleus pulposus (NP) were incubated separately in tissue culture flasks with...

A novel approach to evaluate the extent and the effect of cross-contribution to the intensity of ions designating the analyte and the internal standard in quantitative GC-MS analysis

In gas chromatography-mass spectrometry methods of analysis adopting the analyte’s isotopic analog as the internal standard (IS), the cross-contribution (CC) phenomenon—contribution of IS to the intensities of the ions designating the analyte, and vice versa—has been demonstrated to affect the quantitation data. A novel approach based on the deviations of the empirically observed...

A novel strategy for MALDI-TOF MS analysis of small molecules

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) does not work efficiently on small molecules (usually with molecular weight below 500 Da) because of the interference of matrix-related peaks in low m/z region. The previous methods developed for this problem focused on reducing the peaks caused by the traditional matrices. Here, we report...

A pri-miR-218 variant and risk of cervical carcinoma in Chinese women

Background MicroRNA (miRNA)-related single nucleotide polymorphisms (SNPs) may compromise miRNA binding affinity and modify mRNA expression levels of the target genes, thus leading to cancer susceptibility. However, few studies have investigated roles of miRNA-related SNPs in the etiology of cervical carcinoma. Methods In this case–control study of 1,584 cervical cancer cases and...

A protein kinase C/protein kinase D pathway protects LNCaP prostate cancer cells from phorbol ester-induced apoptosis by promoting ERK1/2 and NF-κB activities

Phorbol esters such as phorbol 12-myristate 13-acetate (PMA) induce apoptosis in many tumor cells including the androgen-sensitive LNCaP prostate cancer cells. Although phorbol ester-induced apoptotic pathways have been well characterized, little is known of the pro-survival pathways modulated by these agents. We now provide experimental evidence to indicate that protein kinase D...