triplicates. Data shown were representative of three biological replicates with standard deviation as error bars. Notes Junshu Wang and Fapeng Liu contributed equally to this work Abbreviations ARTP
Background For metabolic engineering, many rate-limiting steps may exist in the pathways of accumulating the target metabolites. Increasing copy number of the desired genes in these pathways is a general method to solve the problem, for example, the employment of the multi-copy plasmid-based expression system. However, this method may bring genetic instability, structural...
