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21 papers found.
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Analytic expressions of amplitudes by the cross-ratio identity method

In order to obtain the analytic expression of an amplitude from a generic CHY-integrand, a new algorithm based on the so-called cross-ratio identities has been proposed recently. In this paper, we apply this new approach to a variety of theories including the non-linear sigma model, special Galileon theory, pure Yang–Mills theory, pure gravity, Born–Infeld theory, Dirac–Born–Infeld ...

Derivation of Feynman rules for higher order poles using cross-ratio identities in CHY construction

In order to generalize the integration rules to general CHY integrands which include higher order poles, algorithms are proposed in two directions. One is to conjecture new rules, and the other is to use the cross-ratio identity method. In this paper, we use the cross-ratio identity approach to re-derive the conjectured integration rules involving higher order poles for several ...

Cardiac BIN1 (cBIN1) is a regulator of cardiac contractile function and an emerging biomarker of heart muscle health

In recent decades, a cardiomyocyte membrane scaffolding protein bridging integrator 1 (BIN1) has emerged as a critical multifunctional regulator of transverse-tubule (t-tubule) function and calcium signaling in cardiomyocytes. Encoded by a single gene with 20 exons that are alternatively spliced, more than ten BIN1 protein isoforms are expressed with tissue and disease specificity. ...

Magnitude and influencing factors of respiration-induced liver motion during abdominal compression in patients with intrahepatic tumors

Purpose The purpose of this study was to use 4-dimensional-computed tomography (4D-CT) to evaluate respiration-induced liver motion magnitude and influencing factors in patients with intrahepatic tumors undergoing abdominal compression. Methods From January 2012 to April 2016, 99 patients with intrahepatic tumors were included in this study. They all underwent 4D-CT to assess ...

General tree-level amplitudes by factorization limits

To find boundary contributions is a rather difficult problem when applying the BCFW recursion relation. In this paper, we propose an approach to bypass this problem by calculating general tree amplitudes that contain no polynomial using factorization limits. More explicitly, we construct an expression iteratively, which produces the correct factorization limits for all physical ...

The \( \mathcal{Q} \) -cut representation of one-loop integrands and unitarity cut method

Recently, a new construction for complete loop integrands of massless field theories has been proposed, with on-shell tree-level amplitudes delicately incorporated into its algorithm. This new approach reinterprets integrands in a novel form, namely the \( \mathcal{Q} \)-cut representation. In this paper, by deriving one-loop integrands as examples, we elaborate in details the ...

On multi-step BCFW recursion relations

In this paper, we extensively investigate the new algorithm known as the multi-step BCFW recursion relations. Many interesting mathematical properties are found and understanding these aspects, one can find a systematic way to complete the calculation of amplitude after finite, definite steps and get the correct answer, without recourse to any specific knowledge from field ...

Numerical simulation of reservoir parameters’ synergetic time-variability on development rules

Time variability of reservoir parameters in water flooding has an effect on oilfield development rules. Meanwhile, time variability of different reservoir macro-parameters has certain synergetic relationship with each other. Based on microscopic network simulation and reservoir numerical simulation, a new simulation method is presented, which can describe the influence of reservoir ...

Emergence of human caliciviruses among diarrhea cases in southwest China

Background Acute diarrhea is one of the most serious problems in global public health that causes considerable morbidity and mortality worldwide. Human caliciviruses (HuCV) including norovirus (NoV, genogroup GI and GII) and sapovirus (SaV), is a leading cause of acute sporadic diarrhea in individuals across all age groups. However, few studies had been conducted clarifying the ...

Determination of boundary contributions in recursion relation

In this paper, we propose a new algorithm to systematically determine the missing boundary contributions, when one uses the BCFW on-shell recursion relation to calculate tree amplitudes for general quantum field theories. After an instruction of the algorithm, we will use several examples to demonstrate its application, including amplitudes of color-ordered ϕ 4 theory, Yang-Mills ...

Structures of an all-α protein running along the DNA major groove

Despite over 3300 protein–DNA complex structures have been reported in the past decades, there remain some unknown recognition patterns between protein and target DNA. The silkgland-specific transcription factor FMBP-1 from the silkworm Bombyx mori contains a unique DNA-binding domain of four tandem STPRs, namely the score and three amino acid peptide repeats. Here we report three ...

Cell-Cell Adhesions and Cell Contractility Are Upregulated upon Desmosome Disruption

Desmosomes are perturbed in a number of disease states – including genetic disorders, autoimmune and bacterial diseases. Here, we report unexpected changes in other cell-cell adhesion structures upon loss of desmosome function. We found that perturbation of desmosomes by either loss of the core desmosomal protein desmoplakin or treatment with pathogenic anti-desmoglein 3 (Dsg3) ...

Integral reduction by unitarity method for two-loop amplitudes: a case study

In this paper, we generalize the unitarity method to two-loop diagrams and use it to discuss the master integrals of reduction. To test out method, we focus on the four-point double-box diagram as well as its related daughter diagrams, i.e., the doubletriangle diagram and the triangle-box diagram. For later two kinds of diagrams, we have given complete analytical results in general ...

A review of closed-loop reservoir management

The closed-loop reservoir management technique enables a dynamic and real-time optimal production schedule under the existing reservoir conditions to be achieved by adjusting the injection and production strategies. This is one of the most effective ways to exploit limited oil reserves more economically and efficiently. There are two steps in closed-loop reservoir management: ...

Combinatorial Engineering of 1-Deoxy-D-Xylulose 5-Phosphate Pathway Using Cross-Lapping In Vitro Assembly (CLIVA) Method

The ability to assemble multiple fragments of DNA into a plasmid in a single step is invaluable to studies in metabolic engineering and synthetic biology. Using phosphorothioate chemistry for high efficiency and site specific cleavage of sequences, a novel ligase independent cloning method (cross-lapping in vitro assembly, CLIVA) was systematically and rationally optimized in E. ...

Enhancing solubility of deoxyxylulose phosphate pathway enzymes for microbial isoprenoid production

Background Recombinant proteins are routinely overexpressed in metabolic engineering. It is well known that some over-expressed heterologous recombinant enzymes are insoluble with little or no enzymatic activity. This study examined the solubility of over-expressed homologous enzymes of the deoxyxylulose phosphate pathway (DXP) and the impact of inclusion body formation on ...

Metabolite Profiling Identified Methylerythritol Cyclodiphosphate Efflux as a Limiting Step in Microbial Isoprenoid Production

Isoprenoids are natural products that are all derived from isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). These precursors are synthesized either by the mevalonate (MVA) pathway or the 1-Deoxy-D-Xylulose 5-Phosphate (DXP) pathway. Metabolic engineering of microbes has enabled overproduction of various isoprenoid products from the DXP pathway including ...

Statistical Experimental Design Guided Optimization of a One-Pot Biphasic Multienzyme Total Synthesis of Amorpha-4,11-diene

In vitro synthesis of chemicals and pharmaceuticals using enzymes is of considerable interest as these biocatalysts facilitate a wide variety of reactions under mild conditions with excellent regio-, chemo- and stereoselectivities. A significant challenge in a multi-enzymatic reaction is the need to optimize the various steps involved simultaneously so as to obtain high-yield of a ...

Combining Genotype Improvement and Statistical Media Optimization for Isoprenoid Production in E. coli

Isoprenoids are a large and diverse class of compounds that includes many high value natural products and are thus in great demand. To meet the increasing demand for isoprenoid compounds, metabolic engineering of microbes has been used to produce isoprenoids in an economical and sustainable manner. To achieve high isoprenoid yields using this technology, the availability of ...

Novel reference genes for quantifying transcriptional responses of Escherichia coli to protein overexpression by quantitative PCR

Background Accurate interpretation of quantitative PCR (qPCR) data requires normalization using constitutively expressed reference genes. Ribosomal RNA is often used as a reference gene for transcriptional studies in E. coli. However, the choice of reliable reference genes has not been systematically validated. The objective of this study is to identify a set of reliable reference ...