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Direct targets of pSTAT5 signalling in erythropoiesis

primers for ChIP validation. (DOCX) S4 Table. qRT-PCR primers for gene expression validation. (DOCX) Author Contributions Conceptualization: Andrew C. Perkins. Data curation: Kevin R. Gillinder, Charles ... C. Bell, Graham W. Magor, Stephen Huang. Formal analysis: Kevin R. Gillinder, Hugh Tuckey, Graham W. Magor, Andrew C. Perkins. Investigation: Kevin R. Gillinder, Melissa D. Ilsley. Methodology: Kevin

A high-throughput screening strategy for detecting CRISPR-Cas9 induced mutations using next-generation sequencing

Background CRISPR-Cas9 is a revolutionary genome editing technique that allows for efficient and directed alterations of the eukaryotic genome. This relatively new technology has already been used in a large number of ‘loss of function’ experiments in cultured cells. Despite its simplicity and efficiency, screening for mutated clones remains time-consuming, laborious and/or ...

Human Epidermal Neural Crest Stem Cells (hEPI-NCSC)—Characterization and Directed Differentiation into Osteocytes and Melanocytes

Here we describe the isolation, characterisation and ex-vivo expansion of human epidermal neural crest stem cells (hEPI-NCSC) and we provide protocols for their directed differentiation into osteocytes and melanocytes. hEPI-NCSC are neural crest-derived multipotent stem cells that persist into adulthood in the bulge of hair follicles. Multipotency and self-renewal were determined ...