NagC and Mlc, paralogous members of the ROK family of proteins with almost identical helix-turn-helix DNA binding motifs, specifically regulate genes for transport and utilization of N-acetylglucosamine and glucose. We previously showed that two amino acids in a linker region outside the canonical helix-turn-helix motif are responsible for Mlc site specificity. In this work we...
β-lactoglobulin (BLG) is an abundant milk protein relevant for industry and biotechnology, due significantly to its ability to bind a wide range of polar and apolar ligands. While hydrophobic ligand sites are known, sites for hydrophilic ligands such as the prevalent milk sugar, lactose, remain undetermined. Through the use of molecular docking we first, analyzed the known fatty...
Eukarya pyruvate kinases have glutamate at position 117 (numbered according to the rabbit muscle enzyme), whereas in Bacteria have either glutamate or lysine and in Archaea have other residues. Glutamate at this position makes pyruvate kinases K+-dependent, whereas lysine confers K+-independence because the positively charged residue substitutes for the monovalent cation charge...
In the primitive atmosphere, pyrophosphate could have been the energy carrier molecule that gave rise to ATP. During evolution, ATP substituted pyrophosphate because it showed a higher functional versatility. At the same time, the systems that carried out its synthesis or hydrolysis, such as the H+ pyrophosphatases, ATPases and mitocondrial ATP synthases showed a higher...
In the primitive atmosphere, pyrophosphate could have been the energy carrier molecule that gave rise to ATP. During evolution, ATP substituted pyrophosphate because it showed a higher functional versatility. At the same time, the systems that carried out its synthesis or hydrolysis, such as the H+ pyrophosphatases, ATPases and mitocondrial ATP synthases showed a higher...
In the primitive atmosphere, pyrophosphate could have been the energy carrier molecule that gave rise to ATP. During evolution, ATP substituted pyrophosphate because it showed a higher functional versatility. At the same time, the systems that carried out its synthesis or hydrolysis, such as the H+ pyrophosphatases, ATPases and mitocondrial ATP synthases showed a higher...
In the primitive atmosphere, pyrophosphate could have been the energy carrier molecule that gave rise to ATP. During evolution, ATP substituted pyrophosphate because it showed a higher functional versatility. At the same time, the systems that carried out its synthesis or hydrolysis, such as the H+ pyrophosphatases, ATPases and mitocondrial ATP synthases showed a higher...
