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34 papers found.
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Activation of M1 macrophages in sepsis-induced acute kidney injury in response to heparin-binding protein

in the kidney were determined by Western blotting. (TIF) Author Contributions Conceptualization: Li Xing, Mu Genhua. Data curation: Li Xing, Chen Pingfa, He Lei, Jin Qin, Mu Genhua. Formal analysis ... : Li Xing, Song Chunmei. Funding acquisition: Li Xing, Lu Zhongqian, Deng Yijun. Methodology: Li Xing, Lu Zhongqian, Song Chunmei. Project administration: Li Xing, Lu Zhongqian, Deng Yijun. Resources

Potential high-frequency off-target mutagenesis induced by CRISPR/Cas9 in <Emphasis Type=

Hui-Li Xing contributed equally to this work. debate on the function of ABP1 have been resolved through CRISPR/Cas9 (Gao et  al. 2015) . Further analysis of the T-DNA insertion mutant of ABP1 (abp1-1

Direction-of-arrival and Range Estimation of Wideband Sources in the Near Field

In this letter, we address the problem of wideband sources localization in the near field of the sensor array. It is shown that the spatial sampling rate can be considered as a function of temporal frequency so that wideband sources can be represented in the spatial frequency domain. A computationally efficient localization method is then developed based on the Toeplitz matrices...

Effect of YH0618 soup on chemotherapy-induced toxicity in patients with cancer who have completed chemotherapy: study protocol for a randomized controlled trial

Background The incidence of cancer has been staying at a high level worldwide in recent years. With advances in cancer diagnosis and therapy strategy, the survival rate of patients with cancer has been increasing, but the side effects of these treatments, especially chemotherapy, are obvious even when the chemotherapy ceases. YH0618, a prescription, has showed efficacy in...

Repetitive RNA unwinding by RNA helicase A facilitates RNA annealing

Helicases contribute to diverse biological processes including replication, transcription and translation. Recent reports suggest that unwinding of some helicases display repetitive activity, yet the functional role of the repetitiveness requires further investigation. Using single-molecule fluorescence assays, we elucidated a unique unwinding mechanism of RNA helicase A (RHA...

The role of A-kinase anchoring protein 95-like protein in annealing of tRNALys3 to HIV-1 RNA

Background RNA helicase A (RHA), a DExH box protein, promotes annealing of tRNALys3, a primer for reverse transcription, to HIV-1 RNA and assembles into virus particles. A-kinase anchoring protein 95-like protein (HAP95) is a binding partner of RHA. The role of HAP95 in the annealing of tRNALys3 was examined in this study. Results HAP95 associates with the reverse transcriptase...

Application effect of extensively hydrolyzed milk protein formula and follow-up in preterm children with a gestational age of less than 34 weeks: study protocol for a randomized controlled trial

controlled trial Li-Ping Yin 0 Li-Juan Qian 0 Huan Zhu Yan Chen Han Li Ji-Nan Han Li-Xing Qiao 0 Equal contributors Department of Paediatrics, Zhongda Hospital Southeast University , 87 Dingjiaqiao, Nanjing ... University, China, for his help in the design of this trial. We also appreciate the constructive advice from Prof. Hongli Tang from Zhongda Hospital for the ethical review of this study. Li-Xing Qiao is funded

A Functional Role of Fibroblast Growth Factor Receptor 1 (FGFR1) in the Suppression of Influenza A Virus Replication

Influenza A virus causes annual epidemics and occasional pandemics in humans. Here, we investigated four members of the fibroblast growth factor receptor (FGFR) family; FGFR1 to 4, and examined their expression patterns in human lung epithelial cells A549 with influenza A virus infection. We identified a functional role of FGFR1 in influenza A/Puerto Rico/8/1934 (PR8) and A/Anhui...

Cystatin C Shifts APP Processing from Amyloid-β Production towards Non-Amyloidgenic Pathway in Brain Endothelial Cells

Amyloid-β (Aβ), the major component of neuritic plaques in Alzheimer’s disease (AD), is derived from sequential proteolytic cleavage of amyloid protein precursor (APP) by secretases. In this study, we found that cystatin C (CysC), a natural cysteine protease inhibitor, is able to reduce Aβ40 secretion in human brain microvascular endothelial cells (HBMEC). The CysC-induced Aβ40...

Egg cell-specific promoter-controlled CRISPR/Cas9 efficiently generates homozygous mutants for multiple target genes in Arabidopsis in a single generation

Hui-Li Xing 0 Li Dong Hai-Yan Zhang Chun-Yan Han Xue-Chen Wang Qi-Jun Chen 0 Equal contributors State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China

Mesenchymal stem cells, not conditioned medium, contribute to kidney repair after ischemia-reperfusion injury

Introduction Studies have shown that stem cells exert their therapeutic effects on acute kidney injury (AKI) through paracrine/endocrine actions. If the protective effect is mediated in an endocrine manner, the injection of the factors that these cells secrete could be effective, but the effect of conditioned medium (CM) remains controversial. Methods In this study, we cultured...

Roles of the Linker Region of RNA Helicase A in HIV-1 RNA Metabolism

RNA helicase A (RHA) promotes multiple steps in HIV-1 production including transcription and translation of viral RNA, annealing of primer tRNALys3 to viral RNA, and elevating the ratio of unspliced to spliced viral RNA. At its amino terminus are two double-stranded RNA binding domains (dsRBDs) that are essential for RHA-viral RNA interaction. Linking the dsRBDs to the core...

Immunization with a Live Attenuated H7N9 Influenza Vaccine Protects Mice against Lethal Challenge

The emergence of severe cases of human influenza A (H7N9) viral infection in China in the spring of 2003 resulted in a global effort to rapidly develop an effective candidate vaccine. In this study, a cold-adapted (ca), live attenuated monovalent reassortant influenza H7N9 virus (Ah01/AA ca) was generated using reverse genetics that contained hemagglutinin (HA) and neuraminidase...

Inhibition on Proteasome β1 Subunit Might Contribute to the Anti-Cancer Effects of Fangchinoline in Human Prostate Cancer Cells

Fangchinoline is a bisbenzylisoquinoline alkaloid isolated from Radix Stephaniae tetrandrae S. Moore. Fangchinoline and its structure analogue, tetrandrine, exhibited direct binding affinity with recombinant human proteasome β1 subunit and also inhibited its activity in vitro. In cultured prostate PC-3 cells and LnCap cells, fangchinoline could dose-dependently inhibit cell...

A platform for soybean molecular breeding: the utilization of core collections for food security

Soybean is an important crop not only for human consumption but also for its addition of nitrogen to the soil during crop rotation. China has the largest collection of cultivated soybeans (Glycine max) and wild soybeans (Glycine soja) all over the world. The platform of soybean core, mini core and integrated applied core collections has been developed in the past decade based on...

A CRISPR/Cas9 toolkit for multiplex genome editing in plants

Hui-Li Xing 0 Li Dong 0 Zhi-Ping Wang Hai-Yan Zhang Chun-Yan Han Bing Liu Xue-Chen Wang Qi-Jun Chen 0 Equal contributors State Key Laboratory of Plant Physiology and Biochemistry, College of

Packaging of viral RNAs in virions of adenoviruses

Earlier, we detected viral RNAs packaged in the porcine adenovirus (PAdV) -3 virions. Using Southern blot analysis, we further demonstrated that the viral RNAs were predominantly packaged in CsCl purified mature capsids (containing viral genome) than empty/intermediate capsids. Some of the packaged viral RNAs appear to be polyadenylated. Real-time reverse transcription (RT)-PCR...

T2D@ZJU: a knowledgebase integrating heterogeneous connections associated with type 2 diabetes mellitus

Type 2 diabetes mellitus (T2D), affecting >90% of the diabetic patients, is one of the major threats to human health. A comprehensive understanding of the mechanisms of T2D at molecular level is essential to facilitate the related translational research. Here, we introduce a comprehensive and up-to-date knowledgebase for T2D, i.e. T2D@ZJU. T2D@ZJU contains three levels of...