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Development of a dual-expression vector facilitated with selection-free PCR recombination cloning strategy

The conventional procedure for the construction of recombinant expression vector of a target gene includes PCR cloning and restriction enzyme mediated subcloning, which is time-consuming and sometimes troublesome because of the inefficiency of ligation. A variety of ligase-independent PCR cloning strategies have been developed, but they either involve complicated PCR procedures or ...

Responses of absolute and specific enzyme activity to consecutive application of composted sewage sludge in a Fluventic Ustochrept

Composted sewage sludge (CS) is considered a rich source of soil nutrients and significantly affects the physical, chemical, and biological characteristics of soil, but its effect on specific enzyme activity in soil is disregarded. The present experiment examined the absolute and specific enzyme activity of the enzymes involved in carbon, nitrogen, and phosphorus cycles, the ...

Administration of multipotent mesenchymal stromal cells restores liver regeneration and improves liver function in obese mice with hepatic steatosis after partial hepatectomy

partial hepatectomy Fernando Ezquer Javiera Bahamonde Ya-Lin Huang Marcelo Ezquer Background: The liver has the remarkable capacity to regenerate in order to compensate for lost or damaged hepatic tissue

Transcriptome profiling analysis reveals the role of silique in controlling seed oil content in Brassica napus

Seed oil content is an important agronomic trait in oilseed rape. However, the molecular mechanism of oil accumulation in rapeseeds is unclear so far. In this report, RNA sequencing technique (RNA-Seq) was performed to explore differentially expressed genes in siliques of two Brassica napus lines (HFA and LFA which contain high and low oil contents in seeds, respectively) at 15 and ...

A mutation in the CACNA1C gene leads to early repolarization syndrome with incomplete penetrance: A Chinese family study

Background Early repolarization syndrome (ERS) may be a near-Mendelian or an oligogenic disease; however, no direct evidence has been provided to support this theory. Methods and results We described a large Chinese family with nocturnal sudden cardiac death induced by ERS in most of the young male adults. One missense mutation (p.Q1916R) was found in the major subunit of the ...

A critical base pair in k-turns determines the conformational class adopted, and correlates with biological function

k-turns are commonly-occurring motifs that introduce sharp kinks into duplex RNA, thereby facilitating tertiary contacts. Both the folding and conformation of k-turns are determined by their local sequence. k-turns fall into two conformational classes, called N3 and N1, that differ in the pattern of hydrogen bonding in the core. We show here that this is determined by the basepair ...

Editorial for Chinacom2015 Special Issue

process. Dr. Xin-Lin Huang (S’09-M’12SM’16) is currently an Associate Professor with the Department of Information and Communication Engineering, Tongji University, Shanghai, China. He received the M.E. and

Tamoxifen-Dependent, Inducible Bmp2CreER Drives Selective Recombinase Activity in Early Interdigital Mesenchyme and Digit Collateral Ligaments

During limb development, the interdigital mesenchyme has been proposed to play a signaling role instructing morphogenesis of different digit types, as well as undergoing programmed cell death necessary to free digits in animals not adapted for swimming or flying. We have generated a conditional, tamoxifen-dependent Cre line, Bmp2CreER, which drives highly selective recombination ...

What Happened if Various Kinds of Postconditioning Working on the Preconditioned Ischemic Skin Flaps

Objective: Ischemic pre-conditioning and post-conditioning are useful manipulations to reduce the undesirable effects of ischemia-reperfusion skin flap each. But the impact of post-conditioning on the pre-conditioning skin flap is not manifested. Here we investigated the influence of ischemic post-conditioning in a preconditioned axial pattern skin flap model. Method: We used the ...

First record of the Indo-Pacific humpback dolphins (Sousa chinensis) southwest of Hainan Island, China

Background Populations of Indo-Pacific humpback dolphins (Sousa chinensis) in China were known to be distributed from the Beibu Gulf near the border with Vietnam to the mouth of the Yangtze River. According to existing studies, the waters around Hainan Island, China, were not considered to be part of the humpback dolphins’ distribution. Results In 2014, for the first time, we ...

The combination therapy of high-intensity focused ultrasound with radiotherapy in locally advanced pancreatic carcinoma

Background The aim of the present study is to evaluate the effectiveness of the combined application of high-intensity focused ultrasound (HIFU) and radiotherapy in the treatment of locally advanced pancreatic carcinoma (LAPC). Methods A total number of sixteen patients with LAPC started treatment beginning with HIFU and radiotherapy 1 week after the HIFU treatment. Evaluation of ...

Biomechanical Characteristics of Hand Coordination in Grasping Activities of Daily Living

Hand coordination can allow humans to have dexterous control with many degrees of freedom to perform various tasks in daily living. An important contributing factor to this important ability is the complex biomechanical architecture of the human hand. However, drawing a clear functional link between biomechanical architecture and hand coordination is challenging. It is not ...

Orchid Species Richness along Elevational and Environmental Gradients in Yunnan, China

The family Orchidaceae is not only one of the most diverse families of flowering plants, but also one of the most endangered plant taxa. Therefore, understanding how its species richness varies along geographical and environmental gradients is essential for conservation efforts. However, such knowledge is rarely available, especially on a large scale. We used a database extracted ...

Comparison of smith-petersen osteotomy, pedicular subtraction osteotomy, and poly-segmental wedge osteotomy in treating rigid thoracolumbar kyphotic deformity in ankylosing spondylitis a systematic review and meta-analysis

Background This study aimed to compare Smith-Petersen osteotomy (SPO), poly-segmental wedge osteotomy (PWO) and pedicular subtraction osteotomy (PSO) in patients with rigid thoracolumbar kyphosis primarily caused by ankylosing spondylitis. The efficiency, efficacy and safety of these three osteotomies have not been compared systematically, and no illness-oriented surgical type ...

Fusion of detected multi-channel maternal electrocardiogram (ECG) R-wave peak locations

Background Almost all promising non-invasive foetal ECG extraction methods involve accurately determining maternal ECG R-wave peaks. However, it is not easy to robustly detect accurate R-wave peaks of the maternal ECG component in an acquired abdominal ECG since it often has a low signal-to-noise ratio (SNR), sometimes containing a large foetal ECG component or other noises and ...

Identifying a Neuromedin U Receptor 2 Splice Variant and Determining Its Roles in the Regulation of Signaling and Tumorigenesis In Vitro

Neuromedin U (NMU) activates two G protein-coupled receptors, NMUR1 and NMUR2; this signaling not only controls many physiological responses but also promotes tumorigenesis in diverse tissues. We recently identified a novel truncated NMUR2 derived by alternative splicing, namely NMUR2S, from human ovarian cancer cDNA. Sequence analysis, cell surface ELISA and immunocytochemical ...

Prediction of linear B-cell epitopes of hepatitis C virus for vaccine development

, based on the (b) positive samples and (c) negative samples. Additional file 7: Table S5. The control parameters of IBCGA used. Authors’ contributions Wen-Lin Huang (WLH) and Ming-Ju Tsai (MJT) designed

Chitinase-3-like 1 protein (CHI3L1) locus influences cerebrospinal fluid levels of YKL-40

Background Alzheimer’s disease (AD) pathology appears several years before clinical symptoms, so identifying ways to detect individuals in the preclinical stage is imperative. The cerebrospinal fluid (CSF) Tau/Aβ 42 ratio is currently the best known predictor of AD status and cognitive decline, and the ratio of CSF levels of chitinase-3-like 1 protein (CHI3L1, YKL-40) and amyloid ...

Performance enhancement of multiple-gate ZnO metal-oxide-semiconductor field-effect transistors fabricated using self-aligned and laser interference photolithography techniques

Hsin-Ying Lee 0 Hung-Lin Huang 0 Chun-Yen Tseng 0 Department of Photonics, Research Center Energy Technology and Strategy, Advanced Optoelectronic Technology Center, National Cheng Kung University