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A versatile genetic tool: haploid cells

Haploid cells are excellent tools to study gene function as they contain a single copy of the genome and are thus unable to mask the effect of mutations. Recently, haploid embryonic stem cells, which are capable of self-renewal and potentially differentiating into other cell types despite having only one set of chromosomes, have been established in several species. These unique...

miR-589-5p inhibits MAP3K8 and suppresses CD90 + cancer stem cells in hepatocellular carcinoma

Background Cancer stem cells (CSCs) are important in the tumorigenesis and progression of hepatocellular carcinoma (HCC). MicroRNAs (miRNAs) play crucial roles regulating CD133 + and EpCAM + CSCs in HCC, although it is unclear whether miRNAs regulate CD90 + CSCs in HCC. Methods The miRNA profiles of CD90 + and CD90 - HCC cells were analyzed using a miRNA microarray and...

Haploid embryonic stem cells serve as a new tool for mammalian genetic study

In mammals, all somatic cells carry two sets of chromosomes while haploids are restricted only to gametes and are occasionally found in tumors with genome instability. Mammalian haploid embryonic stem (ES) cells have recently been established successfully in mice and monkeys, from either parthenogenetic or androgenetic haploid embryos. These haploid ES cells maintain haploidy and...

Three dimensional collagen scaffolds promote iPSC induction with higher pluripotency

Strategic Priority Research Program of Chinese Academy of Sciences (Grant No. XDA01020101 to Q.Z.). Jie Hao, Qi Zhou, Qi Gu, He Zhu, Lei Chen, Ling Shuai, Jinhui Fang, Jun Wu, Lei Liu, Wei Li, and Jianwu Dai

Derivation of androgenetic embryonic stem cells from m-carboxycinnamic acid bishydroxamide (CBHA) treated androgenetic embryos

The androgenetic embyronic stem (aES) cells are useful models in studying the effects of imprinted genes on pluripotency maintaining and embryo development. The expression patterns of imprinted genes are significantly different between uniparental derived aES cells and zygote-derived embryonic stem (ES) cells, therefore, the imprinting related cell pluripotency needs further...