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The effect of cavernous nerve traction on erectile function in rats

. Supplemental file-raw blots. (DOCX) Author Contributions Conceptualization: Jihong Liu. Formal analysis: Hao Li, Liping Chen. Funding acquisition: Jihong Liu. Investigation: Hao Li. Methodology: Tao Wang ... , Shaogang Wang. Project administration: Tao Wang. Resources: Jihong Liu. 10 / 13 Supervision: Shaogang Wang. Validation: Hao Li. Visualization: Hao Li. Writing ± original draft: Hao Li, Liping Chen

Age-dependent differential regulation of anxiety- and depression-related behaviors by neurabin and spinophilin

. Data curation: Qin Wang. Formal analysis: Huiying Wu, Christopher Cottingham, Liping Chen, Hongxia Wang, Pulin Che, Qin Wang. Investigation: Huiying Wu, Christopher Cottingham, Liping Chen, Hongxia ... Wang, Pulin Che. Methodology: Christopher Cottingham. Resources: Kexiang Liu. Supervision: Qin Wang. Validation: Huiying Wu, Liping Chen, Hongxia Wang, Pulin Che. Visualization: Huiying Wu. Writing

Partial nitritation of stored source-separated urine by granular activated sludge in a sequencing batch reactor

The combination of partial nitritation (PN) and anaerobic ammonium oxidation (anammox) has been proposed as an ideal process for nitrogen removal from source-separated urine, while the high organic matters in urine cause instability of single-stage PN-anammox process. This study aims to remove the organic matters and partially nitrify the nitrogen in urine, producing an ammonium...

Clinical and molecular analyses of norovirus-associated sporadic acute gastroenteritis: the emergence of GII.17 over GII.4, Huzhou, China, 2015

Background Noroviruses (NoVs) are the most common cause of non-bacterial acute gastroenteritis (AGE) in all age groups worldwide. The NoVs circulating in Huzhou over the past 7 years were predominantly GII.4 genotypes. In the winter of 2014–2015, a novel variant of NoV GII.17 emerged and became predominant. We report the epidemiological patterns and genetic characteristics of NoV...

Mining candidate genes associated with powdery mildew resistance in cucumber via super-BSA by specific length amplified fragment (SLAF) sequencing

Background Powdery mildew (PM) is the most common fungal disease of cucumber and other cucurbit crops, while breeding the PM-resistant materials is the effective way to defense this disease, and the recent development of modern genetics and genomics make us aware of that studying the resistance genes is the essential way to breed the PM high-resistance plant. With the ever...

Emergence and predominance of norovirus GII.17 in Huzhou, China, 2014–2015

Background Norovirus (NoV) has been recognized as the leading cause of both outbreaks and sporadic cases of acute gastroenteritis in children and adults worldwide. Stool samples collected from outpatients with clinical symptoms of acute gastroenteritis in all age groups at the First People’s Hospital in Huzhou, Huzhou, China between March 2014 and February 2015 were analyzed to...

Prevalence and genetic diversity of noroviruses in adults with acute gastroenteritis in Huzhou, China, 2013–2014

Norovirus (NoV) infection is the most common cause of nonbacterial acute gastroenteritis, which affects both adults and children. However, the molecular epidemiology of NoV in adults with acute gastroenteritis in China has not been investigated extensively. In this study, we investigated the occurrence of NoV infections and analyzed the genetic diversity of NoV in adults with...

Preparation and Representation of Recombinant Mn-Ferritin Flower-Like Spherical Aggregates from Marine Invertebrates

Ferritin has important functions in the transition and storage of toxic metal ions, but its regulation and function in many invertebrate species are still largely unknown. In our previous work, the cDNA sequence of Sinonovacula constricta, Apostichopus japonicas and Acaudina leucoprocta were constructed and efficiently expressed in E. Coli BL21 under IPTG induction. In this...

Identification and Characterization of Lipase Activity and Immunogenicity of LipL from Mycobacterium tuberculosis

Lipids and lipid-metabolizing esterases/lipases are highly important for the mycobacterial life cycle and, possibly, for mycobacterial virulence. In this study, we expressed 10 members of the Lip family of Mycobacterium tuberculosis. Among the 10 proteins, LipL displayed a significantly high enzymatic activity for the hydrolysis of long-chain lipids. The optimal temperature for...

The role of small RNAs on phenotypes in reciprocal hybrids between Solanum lycopersicum and S. pimpinellifolium

Background Reciprocal hybrids showing different phenotypes have been well documented in previous studies, and many factors accounting for different phenotypes have been extensively investigated. However, less is known about whether the profiles of small RNAs differ between reciprocal hybrids and how these small RNAs affect gene expression and phenotypes. To better understand this...

The role of small RNAs in wide hybridisation and allopolyploidisation between Brassica rapa and Brassica nigra

Background An allopolyploid formation consists of the two processes of hybridisation and chromosome doubling. Hybridisation makes a different genome combined in the same cell, and genome �shock� and instability occur during this process, whereas chromosome doubling results in doubling and reconstructing the genome dosage. Recent studies have demonstrated that small RNAs, play an...

Detection of GI and GII noroviruses in drinking water and vegetables using filtration and real-time RT-PCR

The purpose of the study was to provide a rapid and sensitive method for detecting NoV GI and NoV GII in drinking water and vegetables. The method is based on viral concentration by microporous membrane adsorption method before RNA extraction and real-time RT-PCR amplification. Then water and vegetable samples which artificially contaminated with NoV GI and GII stool samples were...

Genomic Analysis of a Mycobacterium Bovis Bacillus Calmette-Guérin Strain Isolated from an Adult Patient with Pulmonary Tuberculosis

For years, bacillus Calmette-Guérin (BCG) has served as the unique vaccine against tuberculosis and has generally been regarded as safe. However, a clinical strain labeled 3281 that was isolated from a TB patient was identified to be BCG. Via the combination of next-generation sequencing (NGS) and comparative genomic analysis, unique 3281 genetic characteristics were revealed. A...

Rapid Emergence of Novel GII.4 Sub-Lineages Noroviruses Associated with Outbreaks in Huzhou, China, 2008–2012

Infection caused by noroviruses (NoVs) is one of the most important causes of acute gastroenteritis in humans worldwide. To gain insight into the epidemiology of and genetic variation in NoV strains, stool samples collected from 18 outbreaks of acute gastroenteritis in Huzhou, China, between January 2008 and December 2012 were analyzed. Samples were tested for NoVs by real-time...

Heritable variation and small RNAs in the progeny of chimeras of Brassica juncea and Brassica oleracea

Chimeras have been used to study the transmission of genetic material and the resulting genetic variation. In this study, two chimeras, TCC and TTC (where the origin of the outer, middle, and inner cell layers, respectively, of the shoot apical meristem is designated by a ‘T’ for tuber mustard and ‘C’ for red cabbage), as well as their asexual and sexual progeny, were used to...

Note on the Persistent Property of a Discrete Lotka-Volterra Competitive System with Delays and Feedback Controls

A nonautonomous -species discrete Lotka-Volterra competitive system with delays and feedback controls is considered in this work. Sufficient conditions on the coefficients are given to guarantee that all the species are permanent. It is shown that these conditions are weaker than those of Liao et al. 2008.

A Novel Robust Mesh Watermarking Based on BNBW

As a solution to copyright protection of the digital media, digital watermarking techniques have been developed for embedding specific information to identify the owner in the host data imperceptibly. Nowadays, most watermarking methods mainly focused on digital media such as images, video, audio, and text, and very few watermarking methods have been presented for 3D models...

Bioinformatics analysis of the epitope regions for norovirus capsid protein

tools, the bioinformatics pipeline will be more and more critical to vaccine design. Authors contributions Liping Chen, Lei Ji, Xiaofang Wu, Deshun Xu and Jiankang Han carried out the sequences analysis ... and the 3D structures modeling. Di Wu and Zhiwei Cao participated in the possible spatial epitopes predicting. Liping Chen drafted the manuscript. Di Wu helped to draft the manuscript. All authors read

Exome Sequencing Identifies DLG1 as a Novel Gene for Potential Susceptibility to Crohn's Disease in a Chinese Family Study

Background Genetic variants make some contributions to inflammatory bowel disease (IBD), including Crohn’s disease (CD) and ulcerative colitis (UC). More than 100 susceptibility loci were identified in Western IBD studies, but susceptibility gene has not been found in Chinese IBD patients till now. Sequencing of individuals with an IBD family history is a powerful approach toward...