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Search: authors:"Liting Cao"

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Development of a dual-expression vector facilitated with selection-free PCR recombination cloning strategy

The conventional procedure for the construction of recombinant expression vector of a target gene includes PCR cloning and restriction enzyme mediated subcloning, which is time-consuming and sometimes troublesome because of the inefficiency of ligation. A variety of ligase-independent PCR cloning strategies have been developed, but they either involve complicated PCR procedures or ...