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A Versatile Sample Processing Workflow for Metagenomic Pathogen Detection

643476). Author information AffiliationsInstitute of Diagnostic Virology, Friedrich-Loeffler-Institut (FLI), 17493, Greifswald-Insel Riems, GermanyClaudia Wylezich, Martin Beer & Dirk HöperDepartment of ... Papa in:Nature Research journals • PubMed • Google Scholar Search for Martin Beer in:Nature Research journals • PubMed • Google Scholar Search for Dirk Höper in:Nature Research journals • PubMed

A novel European H5N8 influenza A virus has increased virulence in ducks but low zoonotic potential

• Google Scholar Search for Andreas Hocke in:Nature Research journals • PubMed • Google Scholar Search for Thorsten Wolff in:Nature Research journals • PubMed • Google Scholar Search for Martin Beer ... to Donata Hoffmann or Martin Beer. Electronic supplementary material Supplemental Material Rights and permissions Open Access This article is licensed under a Creative Commons Attribution 4.0

Development of molecular confirmation tools for swift and easy rabies diagnostics

As rabies still represents a major public threat with tens of thousands of deaths per year, particularly in developing countries, adequate surveillance based on rapid and reliable rabies diagnosis for both humans and animals is essential. Rabies diagnosis relies on highly sensitive and specific laboratory tests for detection of viral antigens. Among those tests, at present the...

Swarm incursions of reassortants of highly pathogenic avian influenza virus strains H5N8 and H5N5, clade, Germany, winter 2016/17

Höper Günter Strebelow Globig 1 Christoph Staubach 1 Franz J. Conraths Martin Beer 0 , Timm Harder 1 , Thomas C. Mettenleiter OPEN The outbreak of highly pathogenic avian influenza H5Nx viruses in winter

Pedagogical Requirements for Mobile Learning: A Review on MOBIlearn Task Model

Hallam University, GB X close Martin Beer, Sheffield Hallam University, GB X close Paul Crowther Sheffield Hallam University, GB X close Abstract Pedagogically sound

Tick infestation in birds and prevalence of pathogens in ticks collected from different places in Germany

The importance of ticks and tick-borne pathogens for human and animal health has been increasing over the past decades. For their transportation and dissemination, birds may play a more important role than wingless hosts. In this study, tick infestation of birds in Germany was examined. Eight hundred ninety-two captured birds were infested with ticks and belonged to 48 different...

RIEMS: a software pipeline for sensitive and comprehensive taxonomic classification of reads from metagenomics datasets

Background Fuelled by the advent and subsequent development of next generation sequencing technologies, metagenomics became a powerful tool for the analysis of microbial communities both scientifically and diagnostically. The biggest challenge is the extraction of relevant information from the huge sequence datasets generated for metagenomics studies. Although a plethora of tools...

Development and Validation of a Harmonized TaqMan-Based Triplex Real-Time RT-PCR Protocol for the Quantitative Detection of Normalized Gene Expression Profiles of Seven Porcine Cytokines

Expression Profiles of Seven Porcine Cytokines Anja Petrov 0 Martin Beer 0 Sandra Blome 0 Jagadeesh Bayry, Institut National de la Sante et de la Recherche Medicale U 872, France 0 Institute of Diagnostic ... , data collection and analysis, decision to publish, or preparation of the manuscript. Competing Interests: Martin Beer is an Academic Editor for PLOS ONE. This does not alter the authors adherence to

Single immunization with an inactivated vaccine protects sheep from Schmallenberg virus infection

The arthropod-borne Schmallenberg virus (SBV), family Orthobunyaviridae, emerged in Europe in 2011. SBV is associated with a mild disease in adult ruminants but fetal malformation after an infection during a critical phase of pregnancy. A number of inactivated vaccines have been developed; their efficacy after two injections was demonstrated. To make the vaccination of sheep more...

Development and validation of rapid magnetic particle based extraction protocols

Background In order to control and eradicate transboundary animal diseases, early diagnosis and reaction is essential for the implementation of control activities. Thus, mobile diagnostic units which allow analytical testing close to the site of occurrence could provide valuable support for centralized laboratories. Consequently, the availability of diagnostic tests using mobile...

A conserved influenza A virus nucleoprotein code controls specific viral genome packaging

ScholarSearch for Martin Beer in:Nature Research journals • PubMed • Google ScholarSearch for Gert Zimmer in:Nature Research journals • PubMed • Google ScholarSearch for Adolfo García-Sastre in:Nature Research

African swine fever virus transmission cycles in Central Europe: Evaluation of wild boar-soft tick contacts through detection of antibodies against Ornithodoros erraticus saliva antigen

Background African swine fever (ASF) is one of the most complex viral diseases affecting both domestic and wild pigs. It is caused by ASF virus (ASFV), the only DNA virus which can be efficiently transmitted by an arthropod vector, soft ticks of the genus Ornithodoros. These ticks can be part of ASFV-transmission cycles, and in Europe, O. erraticus was shown to be responsible for...

A broad spectrum screening of Schmallenberg virus antibodies in wildlife animals in Germany

To identify native wildlife species possibly susceptible to infection with Schmallenberg virus (SBV), a midge-transmitted orthobunyavirus that predominantly infects domestic ruminants, samples from various free-living ruminants, but also carnivores, small mammals and wild boar were analyzed serologically. Before 2011, no SBV-specific antibodies were detectable in any of the...

Evaluating the protective efficacy of a trivalent vaccine containing Akabane virus, Aino virus and Chuzan virus against Schmallenberg virus infection

Wernike 0 Martin Beer 0 0 Institute of Diagnostic Virology, Friedrich-Loeffler-Institut (FLI) , Suedufer 10, 17493 Greifswald, Insel Riems , Germany Schmallenberg virus (SBV), an arthropod borne pathogen

High resolution melting analysis: rapid and precise characterisation of recombinant influenza A genomes

Donata Kalthoff 0 Martin Beer 0 Bernd Hoffmann 0 0 Institute of Diagnostic Virology, Friedrich-Loeffler-Institut , Sudufer 10, 17493 Greifswald-Insel Riems , Germany Background: High resolution

Comparative analyses of host responses upon infection with moderately virulent Classical swine fever virus in domestic pigs and wild boar

Background Classical swine fever (CSF) is one of the most important viral diseases of pigs. Clinical signs may vary from almost inapparent infection to a hemorrhagic fever like illness. Among the host factors leading to different disease courses are age, breed, and immune status. The aim of this study was to compare host responses of different pig breeds upon infection with a...

Creation of Functional Viruses from Non-Functional cDNA Clones Obtained from an RNA Virus Population by the Use of Ancestral Reconstruction

RNA viruses have the highest known mutation rates. Consequently it is likely that a high proportion of individual RNA virus genomes, isolated from an infected host, will contain lethal mutations and be non-functional. This is problematic if the aim is to clone and investigate high-fitness, functional cDNAs and may also pose problems for sequence-based analysis of viral evolution...

Lack of evidence for the presence of Schmallenberg virus in mosquitoes in Germany, 2011

Background In 2011, a novel orthobunyavirus of the Simbu serogroup was discovered near the German-Dutch border and named Schmallenberg virus (SBV). So far, SBV genome has been detected in various field-collected Culicoides species; however, other members of the Simbu serogroup are also transmitted by mosquitoes. Findings In the present study, approximately 50,000 mosquitoes of...

Influenza A viruses escape from MxA restriction at the expense of efficient nuclear vRNP import

in:Nature Research journals • PubMed • Google ScholarSearch for Martin Beer in:Nature Research journals • PubMed • Google ScholarSearch for Otto Haller in:Nature Research journals • PubMed • Google

Natural Infection of Pregnant Cows with Schmallenberg Virus – A Follow-Up Study

of the manuscript. Competing Interests: Co-author Martin Beer is a PLOS ONE Editorial Board member. This does not alter the authors adherence to all the PLOS ONE policies on sharing data and materials