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Identification and evaluation of reference genes for qRT-PCR studies in Lentinula edodes

, Maolan He, Xiumei Yu, Ke Zhao, Xiaoping Zhang, Menggen Ma, Qiang Chen, Xiaoqiong Chen. 13 / 16 Project administration: Peng Qin. Resources: Xiumei Yu, Ke Zhao, Xianfu Zeng. Supervision: Quanju Xiang

Comparative transcriptome profiling analyses during the lag phase uncover YAP1, PDR1, PDR3, RPN4, and HSF1 as key regulatory genes in genomic adaptation to the lignocellulose derived inhibitor HMF for Saccharomyces cerevisiae

Menggen Ma 0 Z Lewis Liu 0 0 Bioenergy Research Unit, National Center for Agricultural Utilization Research, USDA-ARS , Peoria, IL USA Background: The yeast Saccharomyces cerevisiae is able to

Quantitative transcription dynamic analysis reveals candidate genes and key regulators for ethanol tolerance in Saccharomyces cerevisiae

Background Derived from our lignocellulosic conversion inhibitor-tolerant yeast, we generated an ethanol-tolerant strain Saccharomyces cerevisiae NRRL Y-50316 by enforced evolutionary adaptation. Using a newly developed robust mRNA reference and a master equation unifying gene expression data analyses, we investigated comparative quantitative transcription dynamics of 175 genes...