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Accurate classification of membrane protein types based on sequence and evolutionary information using deep learning

AuthorsSearch for Lei Guo in:PubMed • Google Scholar Search for Shunfang Wang in:PubMed • Google Scholar Search for Mingyuan Li in:PubMed • Google Scholar Search for Zicheng Cao in:PubMed • Google Scholar

Detection of SARS-CoV-2 in fecal samples with different pretreatment methods and PCR kits

information Affiliations West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu, 610041, China Ranran Cao & Mingyuan Li Sichuan Center for Disease Control and Prevention ... publications You can also search for this author in PubMed Google Scholar Yan Li View author publications You can also search for this author in PubMed Google Scholar Mingyuan Li View author

Effects of Highway Landscapes on Drivers’ Eye Movement Behavior and Emergency Reaction Time: A Driving Simulator Study

This study aims at investigating the effects of highway landscapes and alignments on drivers’ eye movement behavior and emergency reaction time, based on a driving simulator experiment. In this study, four simulation scenarios are evaluated including open space, semiopen space, semiclosed space, and enclosed space landscapes on highways in Yunnan Province, China. Twenty-four...

Temporal patterns of influenza A subtypes and B lineages across age in a subtropical city, during pre-pandemic, pandemic, and post-pandemic seasons

Seasonal patterns of influenza A subtypes and B lineages in tropical/subtropical regions across age have remained to be explored. The impact of the 2009 H1N1 pandemic on seasonal influenza activity have not been well understood. Based on a national sentinel hospital-based influenza surveillance system, the epidemiology of influenza virus during 2006/07–2015/16 was characterized...

Relationship between the iceA gene of Helicobacter pylori and clinical outcomes

searched the electronic databases of PubMed , Embase, CNKI, VIP, and Wanfang 1 state Key laboratory of Oral Diseases , Chengdu , People's Republic of China 2 Mingyuan li Department of Microbiology, West

Therapy-Emergent Drug Resistance to Integrase Strand Transfer Inhibitors in HIV-1 Patients: A Subgroup Meta-Analysis of Clinical Trials

Background Integrase strand transfer inhibitors (INSTIs) are a novel class of anti-HIV agents that show high activity in inhibiting HIV-1 replication. Currently, licensed INSTIs include raltegravir (RAL), elvitegravir (EVG) and dolutegravir (DTG); these drugs have played a critical role in AIDS therapy, serving as additional weapons in the arsenal for treating patients infected...

Application of Ferriferous Oxide Modified by Chitosan in Gene Delivery

New approaches to improve the traditional gene carriers are still required. Here we explore Fe3O4 modified with degradable polymers that enhances gene delivery and target delivery using permanent magnetic field. Two magnetic Fe3O4 nanoparticles coated with chitosan (CTS) and polyethylene glycol (PEG) were synthesized by means of controlled chemical coprecipitation. Plasmid pEGFP...

Application of Ferriferous Oxide Modified by Chitosan in Gene Delivery

New approaches to improve the traditional gene carriers are still required. Here we explore Fe3O4 modified with degradable polymers that enhances gene delivery and target delivery using permanent magnetic field. Two magnetic Fe3O4 nanoparticles coated with chitosan (CTS) and polyethylene glycol (PEG) were synthesized by means of controlled chemical coprecipitation. Plasmid pEGFP...

Application of Ferriferous Oxide Modified by Chitosan in Gene Delivery

New approaches to improve the traditional gene carriers are still required. Here we explore Fe3O4 modified with degradable polymers that enhances gene delivery and target delivery using permanent magnetic field. Two magnetic Fe3O4 nanoparticles coated with chitosan (CTS) and polyethylene glycol (PEG) were synthesized by means of controlled chemical coprecipitation. Plasmid pEGFP...

Preparation and Evaluation of Oxaliplatin Thermosensitive Liposomes with Rapid Release and High Stability

Oxaliplatin (OXP) was reported to show low anti-tumor activity when used alone and to display side effects; this low activity was attributed to high partitioning to erythrocytes and low accumulation in tumors. Thermosensitive liposomes (TSL) were considered able to specifically deliver drugs to heated tumors and to resolve the OXP distribution problem. Regretfully, TSL...

A pharmacological analysis of high-affinity sodium transport in barley (Hordeum vulgare L.): a 24Na+/42K+ study

Soil sodium, while toxic to most plants at high concentrations, can be beneficial at low concentrations, particularly when potassium is limiting. However, little is known about Na+ uptake in this ‘high-affinity’ range. New information is provided here with an insight into the transport characteristics, mechanism, and ecological significance of this phenomenon. High-affinity Na...

Rheological properties of polymer micro-gel dispersions

The influence of swelling time, temperature, NaCl concentration and polymer micro-gel concentration on rheological properties of polymer micro-gel dispersions was studied by using a HAAKE rheometer. The results showed that with increasing swelling time and NaCl concentration, the polymer micro-gel dispersions changed from a shear-thickening fluid to a Newtonian fluid. The polymer...

Expression of mouse beta defensin 2 in Escherichia coli and its broad-spectrum antimicrobial activity

Mature mouse beta defensin 2 (mBD2) is a small cationic peptide with antimicrobial activity. Here we established a prokaryotic expression vector containing the cDNA of mature mBD2 fused with thioredoxin (TrxA), pET32a-mBD2. The vector was transformed into Escherichia Coli (E. coli) Rosseta-gami (2) for expression fusion protein. Under the optimization of fermentation parameters...

Expression of mouse beta defensin 2 in Escherichia coli and its broad-spectrum antimicrobial activity

Mature mouse beta defensin 2 (mBD2) is a small cationic peptide with antimicrobial activity. Here we established a prokaryotic expression vector containing the cDNA of mature mBD2 fused with thioredoxin (TrxA), pET32a-mBD2. The vector was transformed into Escherichia Coli (E. coli) Rosseta-gami (2) for expression fusion protein. Under the optimization of fermentation parameters...

Influence of polymers on the stability of Gudao crude oil emulsions

The influence of different types and concentrations of polymers on the stability of Gudao crude oil emulsion was investigated by measuring the volume of water separated from the emulsions and the interfacial shear viscosity of the oil/water interfacial film. Experimental results indicate that the simulated water-in-oil emulsion with 40 mg/L of partially hydrolyzed polyacrylamide...

A novel DNA vaccine expressing the Ag85A-HA2 fusion protein provides protection against influenza A virus and Staphylococcus aureus

Secondary pneumonia due to Staphylococcus aureus (S. aureus) causes significant morbidity and mortality. The aim of the research was designed a novel DNA vaccine encoding the Mycobacterium tuberculosis secreted antigen Ag85A fused with the influenza A virus (IAV) HA2 protein to provide protection against both influenza and secondary infection with S. aureus. The DNA vaccine...