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Assessment of viral methylation levels for high risk HPV types by newly designed consensus primers PCR and pyrosequencing

. Formal analysis: Anna Gillio-Tos, Valentina Fiano, Chiara Grasso, Morena Trevisan, Laura De Marco, Guglielmo Ronco. 13 / 16 Funding acquisition: Guglielmo Ronco. Investigation: Valentina Fiano, Chiara ... Grasso, Morena Trevisan. Methodology: Anna Gillio-Tos, Valentina Fiano, Chiara Grasso, Morena Trevisan, Laura De Marco. Project administration: Anna Gillio-Tos, Guglielmo Ronco. Resources: Guglielmo

Performance of Different Analytical Software Packages in Quantification of DNA Methylation by Pyrosequencing

Background Pyrosequencing has emerged as an alternative method of nucleic acid sequencing, well suited for many applications which aim to characterize single nucleotide polymorphisms, mutations, microbial types and CpG methylation in the target DNA. The commercially available pyrosequencing systems can harbor two different types of software which allow analysis in AQ or CpG mode...

Prenatal Paracetamol Exposure and Wheezing in Childhood: Causation or Confounding?

Background Several studies have reported an increased risk of wheezing in the children of mothers who used paracetamol during pregnancy. We evaluated to what extent this association is explained by confounding. Methods We investigated the association between maternal paracetamol use in the first and third trimester of pregnancy and ever wheezing or recurrent wheezing/asthma in...

Seasonality Modifies Methylation Profiles in Healthy People

DNA methylation is a well-characterized epigenetic modification that plays an important role in the regulation of gene expression. There is growing evidence on the involvement of epigenetic mechanisms in disease onset, including cancer. Environmental factors seem to induce changes in DNA methylation affecting human health. However, little is known about basal methylation levels...

Case–control study of HLA-G promoter methylation status, HPV infection and cervical neoplasia in Curitiba, Brazil: a pilot analysis

Background The causal association between persistent human papillomavirus (HPV) infection and cervical cancer has been established, but the mechanisms that favor HPV persistence in cervical cells are still unknown. The diminished capability of the immune system to control and resolve HPV infection is one of several hypotheses. The tolerogenic protein HLA-G has shown aberrant...

Prevalence and Follow-Up of Occult HCV Infection in an Italian Population Free of Clinically Detectable Infectious Liver Disease

Background Occult hepatitis C virus infection (OCI) is a recently described phenomenon characterized by undetectable levels of HCV-RNA in serum/plasma by current laboratory assays, with identifiable levels in peripheral blood mononuclear cells (PBMCs) and/or liver tissue by molecular tests with enhanced sensitivity. Previous results from our group showed an OCI prevalence of 3.3...

Piccolipiù, a multicenter birth cohort in Italy: protocol of the study

Background The fetal and infant life are periods of rapid development, characterized by high susceptibility to exposures. Birth cohorts provide unique opportunities to study early-life exposures in association with child development and health, as well as, with longer follow-up, the early life origin of adult diseases. Piccolipiù is an Italian birth cohort recently set up to...