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266 papers found.
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Efficacy and long-term longitudinal follow-up of bone marrow mesenchymal cell transplantation therapy in a diabetic patient with recurrent lower limb bullosis diabeticorum

Bullosis diabeticorum is a rare presentation of cutaneous manifestation most commonly affecting the lower limbs in patients with diabetes. The appearance, often as insidious as its resolution, is characterized by tense blisters on the skin surfaces of the lower limbs and the feet. The cause still remains unclear, but it may relate to microangiopathy and neuropathy. In this report...

Cell-penetrating peptide-driven Cre recombination in porcine primary cells and generation of marker-free pigs

, Dan Lu, and Xiao Wen for their proficient technical assistance. Author Contributions Conceptualization: Ning Li. Data curation: Qianqian Kang. Formal analysis: Zhaolin Sun. Funding acquisition ... : Xiaoxiang Hu, Ning Li. Investigation: Ming Wang. Methodology: Qianqian Kang, Zhiyuan Zou, Ming Wang. Project administration: Zhaolin Sun, Qiuyan Li, Xiaoxiang Hu, Ning Li. Resources: Zhiyuan Zou. Software

Development and in vivo validation of tissue-engineered, small-diameter vascular grafts from decellularized aortae of fetal pigs and canine vascular endothelial cells

Tissue engineering has emerged as a promising alternative for small-diameter vascular grafts. The aim of this study was to determine the feasibility of using decellularized aortae of fetal pigs (DAFPs) to construct tissue-engineered, small-diameter vascular grafts and to test the performance and application of DAFPs as vascular tissue-engineered scaffolds in the canine arterial...

Assessing the combined effects of climatic factors on spring wheat phenophase and grain yield in Inner Mongolia, China

Zhao. Validation: Feiyu Pu. Writing ± original draft: Junfang Zhao, Yunpeng Li, Jingwen Xu, Ning Li, Yi Zhang, Jianping Guo, Zhihua Pan. Writing ± review & editing: Junfang Zhao, Feiyu Pu, Zhihua Pan

The effects of patient cost sharing on inpatient utilization, cost, and outcome

: Yuan Xu, Ning Li, Mingshan Lu, Elijah Dixon, Robert P. Myers, Rachel J. Data curation: Yuan Xu, Mingshan Lu, Rachel J. Jelley, Hude Quan. Formal analysis: Yuan Xu, Ning Li, Mingshan Lu, Elijah Dixon ... , Rachel J. Jelley, Hude Quan. Methodology: Yuan Xu, Ning Li, Mingshan Lu, Elijah Dixon, Robert P. Myers, Rachel J. Jelley, Hude Quan. Quan. Project administration: Yuan Xu. Supervision: Ning Li

Integration of human papillomavirus 16 in esophageal carcinoma samples

this work; Haie Shen, Ning Li, Suxian Yang, were samples collection, and performed some experiments; Zhanjun Liu, participated in the design of the study, and performed the statistical analysis. All

MEX3C interacts with adaptor-related protein complex 2 and involves in miR-451a exosomal sorting

Addgene. Conceptualization: Colin E. Bishop, Xiangxian Chen, Baisong Lu. 22 / 25 Formal analysis: Baisong Lu. Investigation: Pin Lu, Huanhuan Li, Ning Li, Ravi N. Singh, Xiangxian Chen, Baisong Lu

Genome-wide analysis of structural variants reveals genetic differences in Chinese pigs

administration: Ruifei Yang, Ran Zhang, Di Liu, Yiqiang Zhao, Xiaoxiang Hu, Ning Li. Supervision: Ran Zhang, Di Liu, Yiqiang Zhao, Xiaoxiang Hu, Ning Li. Visualization: Ruifei Yang. Writing ± original draft

A molasses habitat-derived fungus Aspergillus tubingensis XG21 with high β-fructofuranosidase activity and its potential use for fructooligosaccharides production

The industrial microorganisms used for fructooligosaccharides (FOS) synthesis are generally fermented with sucrose as carbon source to induce the production of β-fructofuranosidase (FFase) having transfructosylation activity. Consequently, isolation of novel FFase producers from a sucrose-enriched biotope would help improve FOS productivity and reduce the process cost. Here...

Optimized double-digest genotyping by sequencing (ddGBS) method with high-density SNP markers and high genotyping accuracy for chickens

High-density single nucleotide polymorphism (SNP) markers are crucial to improve the resolution and accuracy of genome-wide association study (GWAS) and genomic selection (GS). Numerous approaches, including whole genome sequencing, genome sampling sequencing, and SNP chips are able to discover or genotype markers at different densities and costs. Achieving an optimal balance...

The long-term effect of acupuncture for patients with chronic tension-type headache: study protocol for a randomized controlled trial

The effectiveness of acupuncture for patients with chronic tension-type headache (CTTH) is controversial. In this article, we report the protocol for a randomized controlled trial aiming to evaluate the long-term effect of acupuncture for CTTH, in comparison with superficial acupuncture. A two-armed, parallel-design, patient-assessor blind, randomized controlled trial is underway...

Co-Activation of Th17 and Antibody Responses Provides Efficient Protection against Mucosal Infection by Group A Streptococcus

Conserved protein antigens among serotypes of group A Streptococcus pyogenes (GAS) have been focused for vaccine development because of the diversity of GAS serotypes and risks of autoimmunity post-GAS infection. Precise delineation of protective immune response to each of GAS antigens is critical for vaccine efficacy and safety. We recently reported that immunization with SrtA...

The histone variant macroH2A confers functional robustness to the intestinal stem cell compartment

A stem cell’s epigenome directs cell fate during development, homeostasis, and regeneration. Epigenetic dysregulation can lead to inappropriate cell fate decisions, aberrant cell function, and even cancer. The histone variant macroH2A has been shown to influence gene expression, guide cell fate, and safeguard against genotoxic stress. Interestingly, mice lacking functional...

ECL: an exhaustive search tool for the identification of cross-linked peptides using whole database

Background Chemical cross-linking combined with mass spectrometry (CX-MS) is a high-throughput approach to studying protein-protein interactions. The number of peptide-peptide combinations grows quadratically with respect to the number of proteins, resulting in a high computational complexity. Widely used methods including xQuest (Rinner et al., Nat Methods 5(4):315–8, 2008...

Comparison of risk adjustment methods in patients with liver disease using electronic medical record data

Background Risk adjustment is essential for valid comparison of patients’ health outcomes or performances of health care providers. Several risk adjustment methods for liver diseases are commonly used but the optimal approach is unknown. This study aimed to compare the common risk adjustment methods for predicting in-hospital mortality in cirrhosis patients using electronic...

Overexpression of Histone Deacetylase 6 Enhances Resistance to Porcine Reproductive and Respiratory Syndrome Virus in Pigs

. Funding: This research was supported by Genetically modified major projects (Project Grant No. 2011ZX08006) to Professor Ning Li (http:// www.most.gov.cn/kjjh/), the National Natural Scientific Foundation

Pectin enhances the effect of fecal microbiota transplantation in ulcerative colitis by delaying the loss of diversity of gut flora

Background Fecal microbiota transplantation (FMT) induces remission in ulcerative colitis (UC). However, the treatment effect of FMT diminishes over time. Maintaining the diversity of the gut flora for long periods may improve the effects of FMT in UC. Pectin, which can be fermented by gut microbiota into short-chain fatty acids, is postulated to shape the composition and...

Risk assessment and decision-making for patients undergoing orthopedic surgery

Purpose Physical and operative severity score for the enumeration of mortality and morbidity (POSSUM) scoring system was designed to predict the postoperative morbidity and mortality mainly in general surgery. The purpose of this study was to assess the value of POSSUM scoring system in predicting outcomes of patients undergoing orthopedic surgery, and to do some modifications to...

Purification and characterization of recombinant human bile salt-stimulated lipase expressed in milk of transgenic cloned cows

Bile salt-stimulated lipase (BSSL) is a lipolytic digestive enzyme with broad substrate specificity secreted from exocrine pancreas into the intestinal lumen in all species and from the lactating mammary gland into the milk of some species, notably humans but not cows. BSSL in breast milk facilitates digestion and absorption of milk fat and promotes growth of small for...

The impact of predisposing, enabling, and need factors in utilization of health services among rural residents in Guangxi, China

Background Healthcare in China has significantly improved, meanwhile many  socio-economic risk factors and health conditions factors affect accessibility and utilization of health services in rural areas. Inequity of health service in China needs to  be estimated and reduced. Andersen behavioral model is useful to assess the association of health service utilization with...