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Search: authors:"Oliver Fleck"

4 papers found.
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A mutated dph3 gene causes sensitivity of Schizosaccharomyces pombe cells to cytotoxic agents

Dph3 is involved in diphthamide modification of the eukaryotic translation elongation factor eEF2 and in Elongator-mediated modifications of tRNAs, where a 5-methoxycarbonyl-methyl moiety is added to wobble uridines. Lack of such modifications affects protein synthesis due to inaccurate translation of mRNAs at ribosomes. We have discovered that integration of markers at the msh3...

DNA repair

. Oliver Fleck and Olaf Nielsen AA AA XPB TFIH RPA XPD PCNA RFC,RPA LigaseI G =O C Non-homologous end joining (NHEJ) Pol Pold /e Base excision repair (BER) G APendo G Pol In some eukaryotes

Identification of two mismatch-binding activities in protein extracts of Schizosaccharomyces pombe

We have performed band-shift assays to identify mismatch-binding proteins In cell extracts of Schizosaccharomyces pombe. By testing heteroduplex DNA containing either a T/G or a C/C mismatch, two distinct band shifts were produced in the gels. A low mobility complex was observed with the T/G substrate, while a high mobility complex was present with C/C. Further analysis of the...