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Rapid and reliable diagnostic method to detect Zika virus by real-time fluorescence reverse transcription loop-mediated isothermal amplification

Qin Li Wei Wang Jin‑Zhou Wen Lei Zheng 0 Qian Wang 0 0 Laboratory Medicine Center, Nanfang Hospital, Southern Medical University , Guangzhou 510515, Guangdong Province , China 1 Department of Clinical

Modelling the Influence of Awareness Programs by Media on the Drinking Dynamics

, China Received 25 January 2014; Accepted 17 March 2014; Published 16 April 2014 Academic Editor: Yongli Song Copyright © 2014 Hai-Feng Huo and Qian Wang. This is an open access article distributed

Rosiglitazone sensitizes hepatocellular carcinoma cell lines to 5-fluorouracil antitumor activity through activation of the PPARγ signaling pathway

liver cancer. Author contribution Qian WANG designed research; Liang-qi CAO and Xiao-li WANG performed research; Ping XUE, Xing-yuan JIAO, He-ping PENG, Hai-wu LU, and Qiang ZHENG contributed new ... Hospital of Sun Yat-Sen University, Guangzhou 510080, ChinaLiang-qi Cao, Qian Wang, Xi-lin Chen, Xiao-hui Huang, Xin-hui Fu & Jing-song ChenDepartment of Ophthalmology, Guangzhou No.8 People's Hospital

Solutions of the boundary value problem for a 2nth-order nonlinear difference equation containing both advance and retardation

In this paper, we consider the boundary value problem for a 2nth-order nonlinear difference equation containing both advance and retardation. By using the critical point theory, some sufficient conditions of the existence of solutions of the boundary value problem are obtained. The proof is based on the linking theorem. An example is given to illustrate our results.

-Dimensional Duffin-Kemmer-Petiau Oscillator under a Magnetic Field in the Presence of a Minimal Length in the Noncommutative Space

Academic Editor: Sally Seidel Copyright © 2017 Bing-Qian Wang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution

Experimental Research on the Thermal Performance of Composite PCM Hollow Block Walls and Validation of Phase Transition Heat Transfer Models

A type of concrete hollow block with typical structure and a common phase change material (PCM) were adopted. The PCM was filled into the hollow blocks by which the multiform composite PCM hollow blocks were made. The temperature-changing hot chamber method was used to test the thermal performance of block walls. The enthalpy method and the effective heat capacity method were...

A New Method to Simulate Free Surface Flows for Viscoelastic Fluid

Free surface flows arise in a variety of engineering applications. To predict the dynamic characteristics of such problems, specific numerical methods are required to accurately capture the shape of free surface. This paper proposed a new method which combined the Arbitrary Lagrangian-Eulerian (ALE) technique with the Finite Volume Method (FVM) to simulate the time-dependent...

Spread of Carbapenemase-producing Enterobacteria in a Southwest Hospital in China

Background The rapid emergence and dissemination of carbapenem resistance in Enterobacteriaceae complicates the treatment of infections caused by these organisms. Methods We collected clinical isolates with meropenem inhibition zones of ≤ 22 mm from January 1, 2009, through December 31, 2010. We attempted to amplify the NDM-1 gene from these isolates and conducted the modified...

B cell subsets and dysfunction of regulatory B cells in IgG4-related diseases and primary Sjögren’s syndrome: the similarities and differences

Introduction IgG4-related disease (IgG4-RD) is a multisystem-involved autoimmune disease. Abnormally activated and differentiated B cells may play important roles. Regulatory B cells (Breg) are newly defined B cell subgroups with immunosuppressive functions. In this study, we investigated the differences of B cell subsets, the expressions of co-stimulatory molecules on B cells...

c-Fms-mediated differentiation and priming of monocyte lineage cells play a central role in autoimmune arthritis

Introduction Tyrosine kinases are key mediators of multiple signaling pathways implicated in rheumatoid arthritis (RA). We previously demonstrated that imatinib mesylate--a Food and Drug Administration (FDA)-approved, antineoplastic drug that potently inhibits the tyrosine kinases Abl, c-Kit, platelet-derived growth factor receptor (PDGFR), and c-Fms--ameliorates murine...

Chinese Registry of rheumatoid arthritis (CREDIT): II. prevalence and risk factors of major comorbidities in Chinese patients with rheumatoid arthritis

Rheumatoid arthritis patients are at higher risk of developing comorbidities. The main objective of this study was to evaluate the prevalence of major comorbidities in Chinese rheumatoid arthritis patients. We also aimed to identify factors associated with these comorbidities. Baseline demographic, clinical characteristics and comorbidity data from RA patients enrolled in the...

Uncovering packaging features of co-regulated modules based on human protein interaction and transcriptional regulatory networks

Background Network co-regulated modules are believed to have the functionality of packaging multiple biological entities, and can thus be assumed to coordinate many biological functions in their network neighbouring regions. Results Here, we weighted edges of a human protein interaction network and a transcriptional regulatory network to construct an integrated network, and...

Biodegradation of lignin and nicotine with white rot fungi for the delignification and detoxification of tobacco stalk

Background Tobacco stalk is one kind of abundant crop residues in China. The high lignification of tobacco stalk increases its reusing cost and the existing of nicotine will cause serious pollution. The biodegradation of lignocellulosic biomass has been demonstrated to be an environmental and economical approach for the utilization of plant stalk. Meanwhile, many nicotine...

Epithelial ovarian cancer stem-like cells expressing α-gal epitopes increase the immunogenicity of tumor associated antigens

Background As ovarian cancer stem cells (CSCs) are responsible for tumor initiation, invasion, metastasis, and chemo-resistance, new stratagems that selectively target ovarian CSCs are critically significant. Our previous work have demonstrated that ovarian cancer spheroid cells are tumorigenic and chemo-resistant, and have the properties of ovarian CSCs. Herein, we hypothesized...

The PI3K/Akt pathway upregulates Id1 and integrin α4 to enhance recruitment of human ovarian cancer endothelial progenitor cells

Background Endothelial progenitor cells (EPCs) contribute to tumor angiogenesis and growth. We aimed to determine whether inhibitors of differentiation 1 (Id1) were expressed in circulating EPCs of patients with ovarian cancer, whether Id1 could mediate EPCs mobilization and recruitment, and, if so, what underlying signaling pathway it used. Methods Circulating EPCs cultures were...

N-terminal and C-terminal heparin-binding domain polypeptides derived from fibronectin reduce adhesion and invasion of liver cancer cells

Background Fibronectin (FN) is known to be a large multifunction glycoprotein with binding sites for many substances, including N-terminal and C-terminal heparin-binding domains. We investigated the effects of highly purified rhFNHN29 and rhFNHC36 polypeptides originally cloned from the two heparin-binding domains on the adhesion and invasion of highly metastatic human...

Function of nuclear transport factor 2 and Ran in the 20E signal transduction pathway in the cotton bollworm, Helicoverpa armigera

’ contributions Hong-Juan He performed the study. Qian Wang carried out the protein interaction. Wei-Wei Zheng preformed the culture of the HaEpi cell and the RNAi. Jin-Xing Wang and Qi-Sheng Song participated in

Mechanism of nuclear factor of activated T-cells mediated FasL expression in corticosterone -treated mouse Leydig tumor cells

Background Fas and FasL is important mediators of apoptosis. We have previously reported that the stress levels of corticosterone (CORT, glucocorticoid in rat) increase expression of Fas/FasL and activate Fas/FasL signal pathway in rat Leydig cells, which consequently leads to apoptosis. Moreover, our another study showed that nuclear factor of activated T-cells (NFAT) may play a...

Identification and expression profile of a putative basement membrane protein gene in the midgut of Helicoverpa armigera

amount of isopropanol in PBS without RH-2485 was injected. Authors' contributions Jia-Lin Wang carried out immunoblotting. Xiao-Juan Jiang performed Northern blot. Qian Wang completed immunohistochemistry

Genetic linkage of hyperglycemia and dyslipidemia in an intercross between BALB/cJ and SM/J Apoe-deficient mouse strains

Background Individuals with dyslipidemia often develop type 2 diabetes, and diabetic patients often have dyslipidemia. It remains to be determined whether there are genetic connections between the 2 disorders. Methods A female F 2 cohort, generated from BALB/cJ (BALB) and SM/J (SM) Apoe-deficient (Apoe −/− ) strains, was started on a Western diet at 6 weeks of age and maintained...