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Overexpression of CsLEA11, a Y3SK2-type dehydrin gene from cucumber (Cucumis sativus), enhances tolerance to heat and cold in Escherichia coli

As the group II LEA (late embryogenesis abundant) proteins, dehydrins (DHNs) play an important role in plant growth and development, as well as in response to abiotic or biotic stress challenges. In this study, a DHN gene named CsLEA11 was identified and characterized from Cucumis sativus. Sequence analysis of CsLEA11 showed that it is a Y3SK2-type DHN protein rich in hydrophilic ...

Resource allocation for spectrum-leasing based CRN with delay-sensitive traffic

In this paper, cooperative resource allocation strategies are characterized for a spectrum-leasing based cognitive radio network (CRN), where the primary system leases the licensed band to the secondary system for a fraction of time in exchange for the secondary user (SU) acting as relay. Here, both amplify-and-forward (AF) and decode-and-forward (DF) relay protocols are ...

Architecture mode, sedimentary evolution and controlling factors of deepwater turbidity channels: A case study of the M Oilfield in West Africa

Turbidity channels have been considered as one of the important types of deepwater reservoir, and the study of their architecture plays a key role in efficient development of an oil field. To better understand the reservoir architecture of the lower Congo Basin M oilfield, semi-quantitative–quantitative study on turbidity channel depositional architecture patterns in the middle to ...

Local variance of atmospheric 14C concentrations around Fukushima Dai-ichi Nuclear Power Plant from 2010 to 2012

Radiocarbon (14C) has been measured in single tree ring samples collected from the southwest of the Fukushima Dai-ichi Nuclear Power Plant. Our data indicate south-westwards dispersion of radiocarbon and the highest 14C activity observed so far in the local environment during the 2011 accident. The abnormally high 14C activity in the late wood of 2011 ring may imply an unknown ...

The transotic approach for vestibular schwannoma: indications and results

conflict of interest. Author Yi Li declares that she has no conflict of interest. Author Xiaobo Ma declares that he has no conflict of interest. Author Qiang Liu declares that he has no conflict of interest

Effects of astaxanthin on antioxidant capacity of golden pompano (Trachinotus ovatus) in vivo and in vitro

Yun Wang 2 Qiang-qiang Liu 2 0 Institute of Aquatic Economic Animals, School of Life Science, Sun Yat-sen University , NO.135 at Xingang Xi Road, Haizhu District, Guangzhou 510275, Guangdong Province

Transcriptome analysis revealed the dynamic oil accumulation in Symplocos paniculata fruit

Background Symplocos paniculata, asiatic sweetleaf or sapphire berry, is a widespread shrub or small tree from Symplocaceae with high oil content and excellent fatty acid composition in fruit. It has been used as feedstocks for biodiesel and cooking oil production in China. Little transcriptome information is available on the regulatory molecular mechanism of oil accumulation at ...

Efficient biosynthesis of ethyl (R)-4-chloro-3-hydroxybutyrate using a stereoselective carbonyl reductase from Burkholderia gladioli

Background Ethyl (R)-4-chloro-3-hydroxybutyrate ((R)-CHBE) is a versatile chiral precursor for many pharmaceuticals. Although several biosynthesis strategies have been documented to convert ethyl 4-chloro-3-oxobutanoate (COBE) to (R)-CHBE, the catalytic efficiency and stereoselectivity are still too low to be scaled up for industrial applications. Due to the increasing demand of ...

Enantioselective cascade biocatalysis for deracemization of 2-hydroxy acids using a three-enzyme system

Background Enantiopure 2-hydroxy acids are key intermediates for the synthesis of pharmaceuticals and fine chemicals. We present an enantioselective cascade biocatalysis using recombinant microbial cells for deracemization of racemic 2-hydroxy acids that allows for efficient production of enantiopure 2-hydroxy acids. Results The method was realized by a single recombinant ...

Cholesterol metabolism and homeostasis in the brain

constant under normal physical conditions. However, a fraction of the pool is constantly LT 7 R C H D CHC3H3 Juan Zhang, Qiang Liu tc i a m s la m op ulu d c Enreti SI HO CHC3H3 ABC CHC3H3 B B B ... type C protein 2; PD, Parkinsons disease; ZN, zymostenol; ZY, zymosterol. COMPLIANCE WITH ETHICS GUIDELINES Juan Zhang and Qiang Liu declare that they have no conflict of interest. This article does

High-specificity synthesis of novel monomers by remodeled alcohol hydroxylase

Background Diols are important monomers for the production of plastics and polyurethanes, which are widely used in our daily life. The medium-chain diols with one hydroxyl group at its subterminal end are able to confer more flexibility upon the synthesized materials. But unfortunately, this type of diols has not been synthesized so far. The strong need for advanced materials ...

Prognostic significance of Ki67 in Chinese women diagnosed with ER + /HER2 − breast cancers by the 2015 St. Gallen consensus classification

Background This study evaluated the distribution pattern of the Ki67-labeling index (LI) among patients at a Chinese breast cancer center, and analyzed its prognostic significance in the 2015 St Gallen consensus breast cancer classification, estrogen receptor-positive and human epidermal growth factor receptor 2-negative(ER + /HER2 − )subtype. Methods We classified 939 women with ...

The D Domain of LRRC4 anchors ERK1/2 in the cytoplasm and competitively inhibits MEK/ERK activation in glioma cells

Background As a well-characterized key player in various signal transduction networks, extracellular-signal-regulated kinase (ERK1/2) has been widely implicated in the development of many malignancies. We previously found that Leucine-rich repeat containing 4 (LRRC4) was a tumor suppressor and a negative regulator of the ERK/MAPK pathway in glioma tumorigenesis. However, the ...

First report of Rickettsia raoultii and R. slovaca in Melophagus ovinus, the sheep ked

Background Melophagus ovinus (Diptera: Hippoboscidae), a hematophagous ectoparasite, is mainly found in Europe, Northwestern Africa, and Asia. This wingless fly infests sheep, rabbits, and red foxes, and causes inflammation, wool loss and skin damage. Furthermore, this parasite has been shown to transmit diseases, and plays a role as a vector. Herein, we investigated the presence ...

Fine Mapping and Candidate Gene Analysis of the Leaf-Color Gene ygl-1 in Maize

A novel yellow-green leaf mutant yellow-green leaf-1 (ygl-1) was isolated in self-pollinated progenies from the cross of maize inbred lines Ye478 and Yuanwu02. The mutant spontaneously showed yellow-green character throughout the lifespan. Meanwhile, the mutant reduced contents of chlorophyll and Car, arrested chloroplast development and lowered the capacity of photosynthesis ...

A fixed inhaled nitrous oxide/oxygen mixture as an analgesic for adult cancer patients with breakthrough pain: study protocol for a randomized controlled trial

Background The management of breakthrough pain in cancer patients is always a challenge for medical professions. Occurring in 80% of cancer patients with advanced disease, breakthrough pain significantly decreases both patient’s and caregiver’s quality of life. The aim of this study is to assess the analgesic efficacy of a fixed inhaled nitrous oxide/oxygen mixture for adult cancer ...

Effects of a controlled-release fertilizer on yield, nutrient uptake, and fertilizer usage efficiency in early ripening rapeseed (Brassica napus L.)

necessary to determine the effects of the frequency and timing of CRF applications on rapeseed in the future. Compliance with ethics guidelines Chang TIAN, Xuan ZHOU, Qiang LIU, Jian-wei PENG, Wen-ming WANG

First detection of Candidatus Rickettsia barbariae in the flea Vermipsylla alakurt from north-western China

Background Vermipsylla is a genus of the family Vermipsyllidae within the order Siphonaptera of fleas. Vermipsylla alakurt is mainly distributed in alpine pastoral areas of Kazakhstan, Mongolia, China and Nepal, and infests sheep, yaks and horses, causing irritation, poor condition, anaemia and even death. However, to date, no rickettsial agents have been reported in V. alakurt. ...

Dimethyl Fumarate and Monomethyl Fumarate Promote Post-Ischemic Recovery in Mice

Oxidative stress plays an important role in cerebral ischemia–reperfusion injury. Dimethyl fumarate (DMF) and its primary metabolite monomethyl fumarate (MMF) are antioxidant agents that can activate the nuclear factor erythroid-2-related factor 2 (Nrf2)/heme oxygenase-1 (HO-1) pathway and induce the expression of antioxidant proteins. Here, we evaluated the impact of DMF and MMF ...

The Inhibitory Effects of Anacardic Acid on Hepatitis C Virus Life Cycle

Hepatitis C virus (HCV) is a small positive-sense single-stranded RNA virus that causes severe liver diseases. Current anti-HCV therapies involving direct-acting antivirals have significantly enhanced efficacy in comparison to traditional interferon and ribavirin combination. However, further improvement is needed to eradicate HCV. Anacardic acid (AnA) is a phytochemical compound ...