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Oil body biogenesis and biotechnology in legume seeds

230036 , People's Republic of China 1 & Ray J. Rose 2 Communicated by Dr. Neal Stewart 3 School of Environmental and Life Sciences, The University of Newcastle , Callaghan, NSW 2308 , Australia The seeds

RNA processing body (P-body) dynamics in mesophyll protoplasts re-initiating cell division

be mobile in the cytoplasm and show actin-based motility which Ray J Rose facilitates their post-transcriptional role and partitioning to daughter cells. Dedifferentiation; P-bodies; Protoplast

Transcriptional regulation of early embryo development in the model legume Medicago truncatula

Key message Spatial and temporal expression of co-expressed transcription factors provide a framework to investigate the integrated control of embryo size, vascularisation, meristem development and onset of seed filling in early embryogenesis of Medicago truncatula. Abstract Cultivated legumes account for more than a quarter of primary crop production worldwide. The protein- and...

An Unusual Abscisic Acid and Gibberellic Acid Synergism Increases Somatic Embryogenesis, Facilitates Its Genetic Analysis and Improves Transformation in Medicago truncatula

. Rose 0 1 Josep Bassaganya-Riera, Virginia Tech, United States of America 0 Current address: National Institute for Biotechnology and Genetic Engineering , Faisalabad , Pakistan 1 School of Environmental

The 2HA line of Medicago truncatula has characteristics of an epigenetic mutant that is weakly ethylene insensitive

Background The Medicago truncatula 2HA seed line is highly embryogenic while the parental line Jemalong rarely produces embryos. The 2HA line was developed from one of the rare Jemalong regenerates and this method for obtaining a highly regenerable genotype in M. truncatula is readily reproducible suggesting an epigenetic mechanism. Microarray transcriptomic analysis showed down...

Evaluation of Reference Genes for Quantitative Real-Time PCR in Oil Palm Elite Planting Materials Propagated by Tissue Culture

. The MPOB Director-General supported publication of the manuscript and did not influence the content. Competing Interests: Ray J. Rose is employed by the University of Newcastle, Australia. Abdul Munir

Characterisation of the legume SERK-NIK gene superfamily including splice variants: Implications for development and defence

Background SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE (SERK) genes are part of the regulation of diverse signalling events in plants. Current evidence shows SERK proteins function both in developmental and defence signalling pathways, which occur in response to both peptide and steroid ligands. SERKs are generally present as small gene families in plants, with five SERK genes in...

Expression of the SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE1 (SERK1) gene is associated with developmental change in the life cycle of the model legume Medicago truncatula

Kim E. Nolan 0 Sergey Kurdyukov 0 Ray J. Rose 0 0 Australian Research Council Centre of Excellence for Integrative Legume Research, School of Environmental and Life Sciences, University of Newcastle

Root meristems in Medicago truncatula tissue culture arise from vascular-derived procambial-like cells in a process regulated by ethylene

Leaf explants of Medicago truncatula were used to investigate the origins of auxin-induced root formation. On the application of auxin there is some callus formation (not the massive amount that occurs in response to auxin plus cytokinin) and roots appear shortly after the first visible callus. Histological examination reveals morphologically distinctive sheets of callus cells...