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The ATPase motor of the Chd1 chromatin remodeler stimulates DNA unwrapping from the nucleosome

Chromatin remodelers are ATP-dependent motors that reorganize DNA packaging by disrupting canonical histone–DNA contacts within the nucleosome. Here, we show that the Chd1 chromatin remodeler stimulates DNA unwrapping from the edge of the nucleosome in a nucleotide-dependent and DNA sequence-sensitive fashion. Nucleosome binding, monitored by stopped flow, was complex and...

Temperature-dependent Crystallization of MoS2 Nanoflakes on Graphene Nanosheets for Electrocatalysis

This work primarily studies the crystallization condition of molybdenum disulfide (MoS2) in MoS2/graphene hybrids by a temperature-varying hydrothermal method from 150 to 240 °C. Flower-like MoS2 nanoflakes were successfully grown on graphene nanosheets and characterized to understand the temperature-dependent crystallization process and the electrochemical performance. The...

The Chd1 chromatin remodeler can sense both entry and exit sides of the nucleosome

Chromatin remodelers are essential for establishing and maintaining the placement of nucleosomes along genomic DNA. Yet how chromatin remodelers recognize and respond to distinct chromatin environments surrounding nucleosomes is poorly understood. Here, we use Lac repressor as a tool to probe how a DNA-bound factor influences action of the Chd1 remodeler. We show that Chd1...

Revealing editing and SNPs of microRNAs in colon tissues by analyzing high-throughput sequencing profiles of small RNAs

Background Editing and mutations in microRNAs (miRNAs) can change the stability of pre-miRNAs and/or complementarities between miRNAs and their targets. Small RNA (sRNA) high-throughput sequencing (HTS) profiles contain miRNAs that are originated from mutated DNAs or are edited during their biogenesis procedures. It is largely unknown whether miRNAs are edited in colon tissues...

Efficient display of active lipase LipB52 with a Pichia pastoris cell surface display system and comparison with the LipB52 displayed on Saccharomyces cerevisiae cell surface

Anchor System . Biotechnol Prog 2006 , 22 : 989 - 993 . 11. Ren Ren , Jiang Zhengbing , Liu Meiyun , Tao Xingyi , Ma Yushu, Wei Dongzhi : Display of adenoregulin with a novel Pichia pastoris cell surface