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Collecting near mature and immature orchid seeds for ex situ conservation: ‘in vitro collecting’ as a case study

Background Lack of phenological information and efficient collecting methods are considered impediments for orchid seed collecting. This leads to opportunistic collecting as part of general seed collecting schedules that may last few weeks especially in remote areas. The study explored the feasibility of collecting near mature and immature seeds to support conservation action...

Transcriptomic analysis reveals the gene expression profile that specifically responds to IBA during adventitious rooting in mung bean seedlings

Background Auxin plays a critical role in inducing adventitious rooting in many plants. Indole-3-butyric acid (IBA) is the most widely employed auxin for adventitious rooting. However, the molecular mechanisms by which auxin regulate the process of adventitious rooting are less well known. Results The RNA-Seq data analysis indicated that IBA treatment greatly increased the amount...

REG3A promotes the proliferation, migration, and invasion of gastric cancer cells

REG3A promotes the proliferation, migration, and invasion of gastric cancer cells Zhou-Feng Chen, Zhi-Ming Huang, Hai-Bo Xue, Xiu-Qing Lin, Ren-Ping Chen, Meng-Jun Chen, Rui-Fang Jin Department of

De Novo Characterization of the Mung Bean Transcriptome and Transcriptomic Analysis of Adventitious Rooting in Seedlings Using RNA-Seq

Adventitious rooting is the most important mechanism underlying vegetative propagation and an important strategy for plant propagation under environmental stress. The present study was conducted to obtain transcriptomic data and examine gene expression using RNA-Seq and bioinformatics analysis, thereby providing a foundation for understanding the molecular mechanisms controlling...

Fertility-sparing surgery for young patients with borderline ovarian tumors (BOTs): single institution experience

Authors' information Rui-fang Chen,Jun Li, Ting-ting Zhu, Hai-lin Yu, Xin Lu. Department of Gynecologic Oncology, the Obstetrics and Gynecology Hospital of Fudan University , Shanghai , China Background

A Simple Scoring System Predicting the Survival Time of Patients with Bone Metastases after RT

Objectives This study aimed to develop a scoring system to predict the survival time of patients with bone metastases after radiation therapy (RT). The scoring system can guide physicians to a better selection of appropriate treatment regimens. Materials and Methods The medical records of 125 patients with bone metastases treated with RT between January 2007 and September 2010...

Population structure of Haemonchus contortus from seven geographical regions in China, determined on the basis of microsatellite markers

Background Studying genetic variation within and among Haemonchus contortus populations can inform some aspects of this parasite’s population genetics and epidemiology. However, almost nothing is known about such variation in China. Methods Adult males of H. contortus (n = 184) representing seven distinct populations in China were collected, and genetic variation within and among...

Vitamin D Status among Young Children Aged 1–3 Years: A Cross-Sectional Study in Wuxi, China

Background The increasingly recognized importance of vitamin D has been discussed and vitamin D status among young children has attracted widespread attention in recent years. However, study on vitamin D status in young children aged 1–3 y is limited in China. Objective To evaluate the nutritional vitamin D status of young children aged 1–3 y in Wuxi, southeastern China. Methods...

Unplanned pregnancy after ultrasound-guided percutaneous microwave ablation of uterine fibroids: A follow-up study

Hospital, 28 Fuxing Road, Beijing 100853 China.Zhang Bing-song, Zhang Jing, Han Zhi-yu, Xu Chang-tao, Xu Rui-fang, Li Xiu-mei & Liu Hui AuthorsSearch for Zhang Bing-song in:Nature Research journals • PubMed ... Research journals • PubMed • Google ScholarSearch for Xu Rui-fang in:Nature Research journals • PubMed • Google ScholarSearch for Li Xiu-mei in:Nature Research journals • PubMed • Google ScholarSearch for

Comparison of the specificity of antibodies to VAR2CSA in Cameroonian multigravidae with and without placental malaria: a retrospective case–control study

Background Antibodies (Ab) to VAR2CSA prevent Plasmodium falciparum-infected erythrocytes from sequestrating in the placenta, i.e., prevent placental malaria (PM). The specificity of Ab to VAR2CSA associated with absence of PM is unknown. Accordingly, differences in the specificity of Ab to VAR2CSA were compared between multigravidae with and without PM who had Ab to VAR2CSA...

Assessing winter oilseed rape freeze injury based on Chinese HJ remote sensing data

helped improve the original version of the manuscript. Compliance with ethics guidelines Bao SHE, Jing-feng HUANG, Rui-fang GUO, Hong-bin WANG, and Jing WANG declare that they have no conflict of

Identification of host proteins interacting with the integrin-like A domain of Toxoplasma gondii micronemal protein MIC2 by yeast-two-hybrid screening

Background Toxoplasma gondii is an obligate intracellular protozoan, causing the important zoonosis toxoplasmosis. This parasite utilizes a unique form of locomotion called gliding motility to find and invade host cells. The micronemal adhesin MIC2 plays critical roles in these processes by binding to substrates and host cell receptors using its extracellular adhesive domains...

Analysis of the complete Fischoederius elongatus (Paramphistomidae, Trematoda) mitochondrial genome

Background Fischoederius elongates is an important trematode of Paramphistomes in ruminants. Animals infected with F. elongates often don’t show obvious symptoms, so it is easy to be ignored. However it can cause severe economic losses to the breeding industry. Knowledge of the mitochondrial genome of F. elongates can be used for phylogenetic and epidemiological studies. Findings...

Molecular Design, Structural Analysis and Antifungal Activity of Derivatives of Peptide CGA-N46

Chromogranin A (CGA)-N46, a derived peptide of human chromogranin A, has antifungal activity. To further research the active domain of CGA-N46, a series of derivatives were designed by successively deleting amino acid from both terminus of CGA-N46, and the amino acid sequence of each derivative was analyzed by bioinformatic software. Based on the predicted physicochemical...

Fibulin-4 is associated with tumor progression and a poor prognosis in ovarian carcinomas

Background Fibulin-4, a member of the fibulin family of extracellular glycoproteins, is implicated in the progressions of some cancers. However, no information has been available to date regarding the function of fibulin-4 in ovarian carcinoma progression. Methods In this study, fibulin-4 mRNA and protein expression in normal ovarian tissue, ovarian tumor, high invasive subclones...

Two-component dipolar Bose-Einstein condensate in concentrically coupled annular traps

Republic of ChinaXiao-Fei Zhang, Rui-Fang Dong, Hong Chang & Shou-Gang ZhangBeijing National Laboratory for Condensed Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing 100190 ... journals • PubMed • Google ScholarSearch for Lin Wen in:Nature Research journals • PubMed • Google ScholarSearch for Peng Zhang in:Nature Research journals • PubMed • Google ScholarSearch for Rui-Fang Dong

Mitochondrial genome of Hypoderaeum conoideum – comparison with selected trematodes

Background Hypoderaeum conoideum is a neglected but important trematode. The life cycle of this parasite is complex: snails serve as the first intermediate hosts: bivalves, fishes or tadpoles serve as the second intermediate hosts, and poultry (such as chickens and ducks) act as definitive hosts. In recent years, H. conoideum has caused significant economic losses to the poultry...

Optimization of the Expression Conditions of CGA-N46 in Bacillus subtilis DB1342(p-3N46) by Response Surface Methodology

CGA-N46 is a small antifungal-derived peptide and consists of the 31st–76th amino acids of the N-terminus of human chromogranin A. Polycistronic expression of recombinant CGA-N46 in Bacillus subtilis DB1342 was used to improve its production, but the yield of CGA-N46 was still low. In the present study, response surface methodology (RSM) was used to optimize culture medium...

S-1-Based versus Capecitabine-Based Preoperative Chemoradiotherapy in the Treatment of Locally Advanced Rectal Cancer: A Matched-Pair Analysis

Objective The aim of this paper was to compare the efficacy and safety of S-1-based and capecitabine-based preoperative chemoradiotherapy regimens in patients with locally advanced rectal cancer through a retrospective matched-pair analysis. Materials and methods Between Jan 2010 and Mar 2014, 24 patients with locally advanced rectal cancer who received preoperative radiotherapy...