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42 papers found.
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Optically Active Plasmonic Metasurfaces based on the Hybridization of In-Plane Coupling and Out-of-Plane Coupling

Plasmonic metasurfaces have attracted much attention in recent years owing to many promising prospects of applications such as polarization switching, local electric field enhancement (FE), near-perfect absorption, sensing, slow-light devices, and nanoantennas. However, many problems in these applications, like only gigahertz switching speeds of electro-optical switches, low...

High-throughput sequencing of microbial community diversity in soil, grapes, leaves, grape juice and wine of grapevine from China

: Yun Wu, Jie Xue. Validation: Ge Tian, Li-ye Wang. Visualization: Wan Zou, Wen-rui Ma. Writing ± original draft: Yu-jie Wei. Writing ± review & editing: Yu-jie Wei. 14 / 17 15 / 17 16 / 17 1

Selfish routing equilibrium in stochastic traffic network: A probability-dominant description

: Wenyi Zhang. Funding acquisition: Wei Guan. Methodology: Wenyi Zhang, Wei Guan, Rui Ma. Project administration: Zhengbing He. Supervision: Zhengbing He. Writing ± original draft: Wenyi Zhang

Improvement of the catalytic efficiency of a hyperthermophilic xylanase from Bispora sp. MEY-1

, Xiangming Xie, Huiying Luo. Software: Xiaoyu Wang. Supervision: Bin Yao, Xiangming Xie, Huiying Luo. Validation: Shuai You. Writing ± original draft: Xiaoyu Wang. Writing ± review & editing: Rui Ma

Numerical Study of an Efficient Solar Absorber Consisting of Metal Nanoparticles

We propose and theoretically investigate an efficient solar light absorber based on a multilayer structure consisting of tungsten nanoparticle layers and SiO2 layers. According to our calculation, average absorbance over 94% is achieved in the wavelength range between 400 and 2500 nm for the proposed absorber. The excellent performance of the absorber can be attributed to the...

Ultra-narrow Band Perfect Absorber and Its Application as Plasmonic Sensor in the Visible Region

We propose and numerically investigate a perfect ultra-narrowband absorber with an absorption bandwidth of only 1.82 nm and an absorption efficiency exceeding 95% in the visible region. We demonstrate that the perfect ultra-narrowband absorption is ascribed to the coupling effect induced by localized surface plasmon resonance. The influence of structural dimensions on the optical...

Role of N-linked glycosylation in the enzymatic properties of a thermophilic GH 10 xylanase from Aspergillus fumigatus expressed in Pichia pastoris

N-Glycosylation is a posttranslational modification commonly occurred in fungi and plays roles in a variety of enzyme functions. In this study, a xylanase (Af-XYNA) of glycoside hydrolase (GH) family 10 from Aspergillus fumigatus harboring three potential N-glycosylation sites (N87, N124 and N335) was heterologously produced in Pichia pastoris. The N-glycosylated Af-XYNA (WT...

Infrared Perfect Ultra-narrow Band Absorber as Plasmonic Sensor

We propose and numerically investigate a novel perfect ultra-narrow band absorber based on a metal-dielectric-metal-dielectric-metal periodic structure working at near-infrared region, which consists of a dielectric layer sandwiched by a metallic nanobar array and a thin gold film over a dielectric layer supported by a metallic film. The absorption efficiency and ultra-narrow...

Characteristics Peripheral Blood IgG and IgM Heavy Chain Complementarity Determining Region 3 Repertoire before and after Immunization with Recombinant HBV Vaccine

Immunization with recombinant HBV vaccine induces specific immune responses in human causing B lymphocytes to produce protective HBsAb, and to form memory B lymphocytes, thereby facilitating HBV immunity in the body. However, B lymphocytes heterogeneity and characteristics are not fully elucidated. In this study, we conducted high-throughput sequencing of BCR heavy chain CDR3...

Engineering a highly active thermophilic β-glucosidase to enhance its pH stability and saccharification performance

Background β-Glucosidase is an important member of the biomass-degrading enzyme system, and plays vital roles in enzymatic saccharification for biofuels production. Candidates with high activity and great stability over high temperature and varied pHs are always preferred in industrial practice. To achieve cost-effective biomass conversion, exploring natural enzymes, developing...

Revisiting overexpression of a heterologous β-glucosidase in Trichoderma reesei: fusion expression of the Neosartorya fischeri Bgl3A to cbh1 enhances the overall as well as individual cellulase activities

Background The filamentous fungus Trichoderma reesei has the capacity to secret large amounts of cellulase and is widely used in a variety of industries. However, the T. reesei cellulase is weak in β-glucosidase activity, which results in accumulation of cellobiose inhibiting the endo- and exo-cellulases. By expressing an exogenous β-glucosidase gene, the recombinant T. reesei...

Increased hepatic Th2 and Treg subsets are associated with biliary fibrosis in different strains of mice caused by Clonorchis sinensis

Previous studies showed that CD4+T cells responses might be involved in the process of biliary fibrosis. However, the underlying mechanism resulting in biliary fibrosis caused by Clonorchis sinensis remains not yet fully elucidated. The objectives of the present study were to investigate the different profiles of hepatic CD4+T cell subsets (Th1, Th2, Th17 and Treg cells) and...

Identification of the C-Terminal GH5 Domain from CbCel9B/Man5A as the First Glycoside Hydrolase with Thermal Activation Property from a Multimodular Bifunctional Enzyme

Caldicellulosiruptor bescii encodes at least six unique multimodular glycoside hydrolases crucial for plant cell wall polysaccharides degradation, with each having two catalytic domains separated by two to three carbohydrate binding modules. Among the six enzymes, three have one N- or C-terminal GH5 domain with identical amino acid sequences. Despite a few reports on some of...

Short-term assessment of BCR repertoires of SLE patients after high dose glucocorticoid therapy with high-throughput sequencing

We analyze and assess BCR repertoires of SLE patients before and after high dose glucocorticoid therapy to address two fundamental questions: (1) After the treatment, how the BCR repertoire of SLE patient change on the clone level? (2) How to screen putative autoantibody clone set from BCR repertoire of SLE patients? The PBMCs of two SLE patients (P1 and P2) at different time...

An economic and low-carbon day-ahead Pareto-optimal scheduling for wind farm integrated power systems with demand response

) and the source are credited. Rui MA received the B.Sc. degree in Electrical Engineering, the M.Sc. degree in Control Theory and Contol Enginnering, and the Ph.D. degree in Electrical Engineering from

A Novel GH7 Endo-β-1,4-Glucanase from Neosartorya fischeri P1 with Good Thermostability, Broad Substrate Specificity and Potential Application in the Brewing Industry

An endo-β-1,4-glucanase gene, cel7A, was cloned from the thermophilic cellulase-producing fungus Neosartorya fischeri P1 and expressed in Pichia pastoris. The 1,410-bp full-length gene encodes a polypeptide of 469 amino acids consisting of a putative signal peptide at residues 1–20, a catalytic domain of glycoside hydrolase family 7 (GH7), a short Thr/Ser-rich linker and a family...

Improvement of the thermostability and catalytic efficiency of a highly active β-glucanase from Talaromyces leycettanus JCM12802 by optimizing residual charge–charge interactions

Background β-Glucanase is one of the most extensively used biocatalysts in biofuel, food and animal feed industries. However, the poor thermostability and low catalytic efficiency of most reported β-glucanases limit their applications. Currently, two strategies are used to overcome these bottlenecks, i.e., mining for novel enzymes from extremophiles and engineering existing...

Role of estrogen in hepatocellular carcinoma: is inflammation the key?

Hepatocellular carcinoma (HCC) is one of the most common malignancies worldwide and accounts for the third-leading cause of cancer-related deaths. Over the past decades, advances have been made in the field of surgery, but effective treatment of HCC is lacking. Due to a marked male predominance in morbidity and mortality in HCC patients, it has long been considered that sex...

Genes associated with thermosensitive genic male sterility in rice identified by comparative expression profiling

Background Thermosensitive genic male sterile (TGMS) lines and photoperiod-sensitive genic male sterile (PGMS) lines have been successfully used in hybridization to improve rice yields. However, the molecular mechanisms underlying male sterility transitions in most PGMS/TGMS rice lines are unclear. In the recently developed TGMS-Co27 line, the male sterility is based on co...