EHD2 is a mechanotransducing ATPase localized in caveolae invaginations at the plasma membrane. EHD2 has recently been associated with several human cancers, however the significance of EHD2 transcript levels in cancer prognosis remains debated. Breast cancer is the most commonly occurring cancer in women and prognosis is variable depending on the subtypes. Triple negative breast...
) for their technical help. Author Contributions Conceptualization: Sophie Vacher, Ivan Bieche, Martine Perrot-Applanat. Data curation: Sophie Vacher, Martine Perrot-Applanat. Formal analysis: Sophie ... Vacher, Walid Chemlali, Didier Meseure. Funding acquisition: Sophie Vacher, Ivan Bieche, Martine Perrot-Applanat. Investigation: Sophie Vacher, Patrice Castagnet, Walid Chemlali. Methodology: Sophie
Endocrine therapies targeting estrogen signaling have significantly improved breast cancer (BC) patient survival, although 40% of ERα-positive BCs do not respond to those therapies. Aside from genomic signaling, estrogen triggers non-genomic pathways by forming a complex containing methylERα/Src/PI3K, a hallmark of aggressiveness and resistance to tamoxifen. We aimed to confirm...
Predicting the risk of liver metastasis can have important prognostic and therapeutic implications, given the availability of liver-directed therapy. Uveal melanoma has a striking predisposition for liver metastasis despite the absence of anatomical proximity. Understanding its biology may uncover factors promoting liver metastasis in other malignancies. We quantified gene...
, Sophie Vacher, Anais Boulai, Ivan Bièche & Céline CallensClinic bioinformatic Unit, Department of Biopathology, Curie Institute, PSL Research University, Paris, FranceVirginie BernardInstitut Curie ... • Google Scholar Search for Sophie Vacher in:PubMed • Google Scholar Search for Anais Boulai in:PubMed • Google Scholar Search for Virginie Bernard in:PubMed • Google Scholar Search for Sylvain Baulande
Objective: In patients with head and neck squamous cell carcinoma (HNSCC), cetuximab [a monoclonal antibody targeting epidermal growth factor receptor (EGFR)] has been shown to improve overall survival when combined with radiotherapy in the locally advanced setting or with chemotherapy in first-line recurrent and/or metastatic (R/M) setting, respectively. While biomarkers of...
Sophie Vacher in:PubMed • Google Scholar Search for Ivan Bièche in:PubMed • Google Scholar Search for Nathalie Marchand in:PubMed • Google Scholar Search for Yvan de Launoit in:PubMed • Google Scholar
To better define the role of FOXO1 and FOXO3 transcriptional factors in breast carcinogenesis, we performed a comparative study of their expression at both the RNA and protein levels in a series of human breast tumors. We used qRT-PCR assay to quantify mRNA expression and Reverse Phase Protein Arrays (RPPA) to quantify protein expression in 218 breast tumors from patients with...
Kindlin-1, − 2, and − 3 are the three members of the Kindlin family. They are best known as regulators of integrin functions, contributing to fundamental biological processes such as cell survival, adhesion and migration. Their deregulation leads to diverse pathologies including a broad range of cancers in which both, tumor-promoting and tumor-inhibiting functions have been...
François Lallemand Sophie Vacher Rosette Lidereau Ivan Bieche 0 Celine Callens 0 0 Equal contributors Service de Génétique, Unité de Pharmacogénomique, Institut Curie , 26 rue d'ulm, 75005 Paris
National Medical Research Center of Oncology, Moscow, RussiaSvetlana N. RubtsovaDepartment of Genetics, Institut Curie, Paris, FranceAnne Schnitzler, Sophie Vacher & Ivan BiècheTomsk National Research ... • Google Scholar Search for Anne Schnitzler in:Nature Research journals • PubMed • Google Scholar Search for Sophie Vacher in:Nature Research journals • PubMed • Google Scholar Search for Evgeny V. Denisov
The WW domain-containing oxidoreductase (WWOX) gene, frequently altered in breast cancer, encodes a tumor suppressor whose function is mediated through its interactions with cancer-related proteins, such as the pro-apoptotic protein p73α. To better understand the involvement of WWOX in breast tumorigenesis, we performed a yeast two-hybrid screen and co-immunoprecipitation assays...
Background:Vascular endothelial growth factor (VEGF) is a multifunctional cytokine that has important roles in angiogenesis. Our knowledge of the significance of VEGF isoforms in human cancer remains incomplete.Methods:Bioluminescence imaging and transcriptomic analysis were used to study the colonisation capacity of the human breast cancer cells MDA-MB-231 controlling or...
Background:The Arp2/3 complex is required for cell migration and invasion. The Arp2/3 complex and its activators, such as the WAVE complex, are deregulated in diverse cancers. Here we investigate the expression of Arpin, the Arp2/3 inhibitory protein that antagonises the WAVE complex.Methods:We used qRT–PCR and reverse phase protein arrays in a patient cohort with known clinical...
Background The present study focused on the prognostic roles of PIK3CA and PIK3R1 genes and additional PI3K pathway-associated genes in breast cancer. Methods The mutational and mRNA expression status of PIK3CA, PIK3R1 and AKT1, and expression status of other genes involved in the PI3K pathway (EGFR, PDK1, PTEN, AKT2, AKT3, GOLPH3, WEE1, P70S6K) were assessed in a series of 458...
Background The PI3K/AKT pathway plays a pivotal role in breast cancer development and maintenance. PIK3CA, encoding the PI3K catalytic subunit, is the oncogene exhibiting a high frequency of gain-of-function mutations leading to PI3K/AKT pathway activation in breast cancer. PIK3CA mutations have been observed in 30% to 40% of ERα-positive breast tumors. However the...
Introduction PIK3CA is the oncogene showing the highest frequency of gain-of-function mutations in breast cancer, but the prognostic value of PIK3CA mutation status is controversial. Methods We investigated the prognostic significance of PIK3CA mutation status in a series of 452 patients with unilateral invasive primary breast cancer and known long-term outcome (median follow-up...
Background Tumor endothelial transdifferentiation and VEGFR1/2 expression by cancer cells have been reported in glioblastoma but remain poorly documented for many other cancer types. Methods To characterize vasculature of patient-derived tumor xenografts (PDXs), largely used in preclinical anti-angiogenic assays, we designed here species-specific real-time quantitative RT-PCR...
Dailly, Institut Curie - Hôpital Rene Huguenin, St Cloud, FranceIvan Bieche, Sophie Vacher & Rana HatemINSERM UMR745, Sorbonne Paris Cité, 4 avenue de l’Observatoire, Paris, FranceIvan BiecheDépartement de ... Curie, 12 rue Lhomond, F-75005, Paris, FranceVirginie Dangles-Marie AuthorsSearch for Ivan Bieche in:PubMed • Google Scholar Search for Sophie Vacher in:PubMed • Google Scholar Search for David
Background IBC (Inflammatory Breast cancer) is a rare form of breast cancer with a particular phenotype. New molecular targets are needed to improve the treatment of this rapidly fatal disease. Given the role of NF-κB-related genes in cell proliferation, invasiveness, angiogenesis and inflammation, we postulated that they might be deregulated in IBC. Methods We measured the mRNA...
