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A genome-wide dsRNA library screen for Drosophila genes that regulate the GBP/phospholipase C signaling axis that links inflammation to aging

ObjectiveInvertebrates are productive models for understanding how inflammation, metabolism and aging are intertwined. We have deployed a dsRNA library screen to search for genes in Drosophila melanogaster—and hence identify human orthologs—that encode participants in a G-protein coupled, Ca2+-signaling pathway that regulates inflammation, metabolism and lifespan.ResultsWe...

Inositol Pyrophosphate Profiling of Two HCT116 Cell Lines Uncovers Variation in InsP8 Levels

The HCT116 cell line, which has a pseudo-diploid karotype, is a popular model in the fields of cancer cell biology, intestinal immunity, and inflammation. In the current study, we describe two batches of diverged HCT116 cells, which we designate as HCT116NIH and HCT116UCL. Using both gel electrophoresis and HPLC, we show that HCT116UCL cells contain 6-fold higher levels of InsP8...

Retraction: A High-Throughput Screening-Compatible Strategy for the Identification of Inositol Pyrophosphate Kinase Inhibitors

. Stashko Henning J. Jessen Kenneth H. Pearce Stephen V. Frye Stephen B. Shears OPEN ACCESS The authors of the above paper retract this article due to concerns about the integrity of the data and the

A High-Throughput Screening-Compatible Strategy for the Identification of Inositol Pyrophosphate Kinase Inhibitors

Pharmacological tools—‘chemical probes’—that intervene in cell signaling cascades are important for complementing genetically-based experimental approaches. Probe development frequently begins with a high-throughput screen (HTS) of a chemical library. Herein, we describe the design, validation, and implementation of the first HTS-compatible strategy against any inositol phosphate...

Human Genome-Wide RNAi Screen Identifies an Essential Role for Inositol Pyrophosphates in Type-I Interferon Response

M. Riley Richa Tyagi Pradeep D. Uchil John D. York Solomon H. Snyder Adolfo Garca- Sastre Barry V. L. Potter Rongtuan Lin Stephen B. Shears Ramnik J. Xavier Manoj N. Krishnan Karen L. Mossman