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Controlled depolymerisation assessed by analytical ultracentrifugation of low molecular weight chitosan for use in archaeological conservation

, Nottingham NG7 2RD , UK 3 Stephen E. Harding The heterogeneity and molecular weight of a chitosan of low molecular weight (molar mass) and low degree of acetylation (0.1) for potential use as a consolidant

Characterisation of insulin analogues therapeutically available to patients

hydrodynamic aspects of the findings. Author Contributions Conceptualization: Gary G. Adams, Andrew Meal, Paul S. Morgan, M. Samil Kok, Stephen E. Harding. Data curation: Qushmua E. Alzahrani, Hanne Zobel ... , Ryan Lithgo, David T. M. Besong, Shahwar I. Jiwani, Simon Ballance, Stephen E. Harding, Richard B. Gillis. Formal analysis: Gary G. Adams, Andrew Meal, Paul S. Morgan, Hanne Zobel, Ryan Lithgo, M. Samil

Application of novel analytical ultracentrifuge analysis to solutions of fungal mannans

Polysaccharides, the most abundant biopolymers, are required for a host of activities in lower organisms, animals, and plants. Their solution characterization is challenging due to their complex shape, heterogeneity, and size. Here, recently developed data analysis approaches were applied for traditional sedimentation equilibrium and velocity methods in order to investigate the...

Hydrodynamic modelling of protein conformation in solution: ELLIPS and HYDRO

Jos Garca de la Torre 0 Stephen E. Harding 0 0 J. Garca de la Torre Departamento de Quimica Fisica, Universidad de Murcia , Regional Campus Mare Nostrum, 30071 Murcia, Spain 1 ) School of

MultiSig: a new high-precision approach to the analysis of complex biomolecular systems

MultiSig is a newly developed mode of analysis of sedimentation equilibrium (SE) experiments in the analytical ultracentrifuge, having the capability of taking advantage of the remarkable precision (~0.1 % of signal) of the principal optical (fringe) system employed, thus supplanting existing methods of analysis through reducing the ‘noise’ level of certain important parameter...

Activation of 2′ 5′-Oligoadenylate Synthetase by Stem Loops at the 5′-End of the West Nile Virus Genome

West Nile virus (WNV) has a positive sense RNA genome with conserved structural elements in the 5′ and 3′ -untranslated regions required for polyprotein production. Antiviral immunity to WNV is partially mediated through the production of a cluster of proteins known as the interferon stimulated genes (ISGs). The 2′ 5′-oligoadenylate synthetases (OAS) are key ISGs that help to...

Pectic polysaccharides from Biophytum petersianum Klotzsch, and their activation of macrophages and dendritic cells

The Malian medicinal plant Biophytum petersianum Klotzsch (Oxalidaceae) is used as a treatment against various types of illnesses related to the immune system, such as joint pains, inflammations, fever, malaria, and wounds. A pectic polysaccharide obtained from a hot water extract of the aerial parts of B. petersianum has previously been reported to consist of arabinogalactans...

A Multilaboratory Comparison of Calibration Accuracy and the Performance of External References in Analytical Ultracentrifugation

Analytical ultracentrifugation (AUC) is a first principles based method to determine absolute sedimentation coefficients and buoyant molar masses of macromolecules and their complexes, reporting on their size and shape in free solution. The purpose of this multi-laboratory study was to establish the precision and accuracy of basic data dimensions in AUC and validate previously...

Excavating Past Population Structures by Surname-Based Sampling: The Genetic Legacy of the Vikings in Northwest England

. Thomas 0 Stephen E. Harding Mark A. Jobling 2 0 Department of Biology, University College London , London, United Kingdom 1 Present address: Division of GI Surgery, University of Nottingham Medical School

Immunological and Structural Properties of a Pectic Polymer from Glinus Oppositifolius

The aim of this paper was to further elucidate the structure and the immunomodulating properties of the pectic polymer GOA2, previously isolated from Glinus oppositifolius. Enzymatic treatment of GOA2 by endo-α-d-(1→4)-polygalacturonase led to the isolation of three pectic subunits, GOA2-I, GOA2-II, and GOA2-III, in addition to oligogalacturonides. GOA2-I was shown to consist of...