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Analyzing and minimizing PCR amplification bias in Illumina sequencing libraries

Despite the ever-increasing output of Illumina sequencing data, loci with extreme base compositions are often under-represented or absent. To evaluate sources of base-composition bias, we traced genomic sequences ranging from 6% to 90% GC through the process by quantitative PCR. We identified PCR during library preparation as a principal source of bias and optimized the...

Bostrycin inhibits proliferation of human lung carcinoma A549 cells via downregulation of the PI3K/Akt pathway

Background Bostrycin is a novel compound isolated from marine fungi that inhibits proliferation of many cancer cells. However, the inhibitory effect of bostrycin on lung cancers has not been reported. This study is to investigate the inhibitory effects and mechanism of bostrycin on human lung cancer cells in vitro. Methods We used MTT assay, flow cytometry, microarray, real time...

Autoimmune rheumatic diseases increase dementia risk in middle-aged patients: A nationwide cohort study

Chen, Jau-Jiuan Sheu, Yi-Hsuan Chen. Formal analysis: Wei-Sheng Chen, Jau-Jiuan Sheu. Investigation: Jin-Hua Chen. Supervision: Chi-Ching Chang. Writing ± original draft: Tzu-Min Lin, Yi-Hsuan Chen ... overall risk of dementia. The underlying mechanism of these results is not fully understood and warrants further research. Author Contributions Conceptualization: Tzu-Min Lin. Data curation: Wei-Sheng

The Epidermal Growth Factor-like Domain of CD93 Is a Potent Angiogenic Factor

Human CD93, an epidermal growth factor (EGF)-like domain containing transmembrane protein, is predominantly expressed in the vascular endothelium. Studies have shown that AA4, the homolog of CD93 in mice, may mediate cell migration and angiogenesis in endothelial cells. Soluble CD93 has been detected in the plasma of healthy individuals. However, the role of soluble CD93 in the...

Galectin-8 Promotes Cytoskeletal Rearrangement in Trabecular Meshwork Cells through Activation of Rho Signaling

Purpose The trabecular meshwork (TM) cell-matrix interactions and factors that influence Rho signaling in TM cells are thought to play a pivotal role in the regulation of aqueous outflow. The current study was designed to evaluate the role of a carbohydrate-binding protein, galectin-8 (Gal8), in TM cell adhesion and Rho signaling. Methods Normal human TM cells were assayed for...

Galectin-8 Ameliorates Murine Autoimmune Ocular Pathology and Promotes a Regulatory T Cell Response

Galectins have emerged as potent immunoregulatory agents that control chronic inflammation through distinct mechanisms. Here, we report that treatment with Galectin-8 (Gal-8), a tandem-repeat member of the galectin family, reduces retinal pathology and prevents photoreceptor cell damage in a murine model of experimental autoimmune uveitis. Gal-8 treatment increased the number of...

Effects of annual influenza vaccination on morbidity and mortality in patients with Systemic Lupus Erythematosus: A Nationwide Cohort Study

Wei-Sheng Chen in:Nature Research journals • PubMed • Google ScholarSearch for Yi-Hsuan Chen in:Nature Research journals • PubMed • Google ScholarSearch for Jin-Hua Chen in:Nature Research journals ... to be published. Wei-Sheng Chen contributed to the analysis of data, revision of the article critically for crucial intellectual content, and final approval of the version to be published. Yi-Hsuan