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The structure differences of Japanese encephalitis virus SA14 and SA14-14-2 E proteins elucidate the virulence attenuation mechanism

(SSRF) and X10SA (SLS) for assistance during data collection. This work was supported by China, Grant No. 2014ZX09304316-003 and Grant No. 2015DFA30690), and MaxPlanck-Gesellschaft. Xinyu Liu, Xin Zhao ... Commons license, and indicate if changes were made. Xinyu Liu and Xin Zhao have contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org

An Algorithm for the Mixed Transportation Network Design Problem

This paper proposes an optimization algorithm, the dimension-down iterative algorithm (DDIA), for solving a mixed transportation network design problem (MNDP), which is generally expressed as a mathematical programming with equilibrium constraint (MPEC). The upper level of the MNDP aims to optimize the network performance via both the expansion of the existing links and the...

In Situ SEM Nanomanipulation and Nanomechanical/Electrical Characterization

quality of the papers. Yang Lu Yajing Shen Xinyu Liu Mohd Ridzuan Bin Ahmad Yan Chen

Change in the immune function of porcine iliac artery endothelial cells infected with porcine circovirus type 2 and its inhibition on monocyte derived dendritic cells maturation

assistance with flow cytometry analysis. Author Contributions Data curation: Ning Yang, Jinzeng Qiao, Shiyu Liu, Xinyu Liu. Formal analysis: Ning Yang, Shiyu Liu. Funding acquisition: Shuanghai Zhou

Novel SINS Initial Alignment Method under Large Misalignment Angles and Uncertain Noise Based on Nonlinear Filter

For the SINS initial alignment problem under large misalignment angles and uncertain noise, two novel nonlinear filters, referred to as transformed unscented quadrature Kalman filter (TUQKF) and robust transformed unscented quadrature Kalman filter (RTUQKF), are proposed in this paper, respectively. The TUQKF sets new deterministic sigma points to address the nonlocal sampling...

Visualizing Micro-anatomical Structures of the Posterior Cornea with Micro-optical Coherence Tomography

Research Council Singapore (NMRC/CBRG/0036/2013) and NTU-AIT-MUV program in advanced biomedical imaging (NAM/15005). Author information Author notes Si Chen and Xinyu Liu contributed equally to this work ... . AffiliationsSchool of Electrical and Electronic Engineering, Nanyang Technological University, Singapore, 639798, SingaporeSi Chen, Xinyu Liu, Nanshuo Wang, Xianghong Wang, Qiaozhou Xiong, En Bo, Xiaojun Yu, Shufen

Microstructures and microwave dielectric properties of (Ba1−x Sr x )4(Sm0.4Nd0.6)28/3Ti18O54 solid solutions

(Ba1−x Sr x )4(Sm0.4Nd0.6)28/3Ti18O54 (x = 0.02, 0.04, 0.06, 0.08, 0.1) solid solutions were prepared by the conventional solid-state reaction process. It was found that (Ba1−x Sr x )4(Sm0.4Nd0.6)28/3Ti18O54 ceramics are fully composed of BaSm2Ti4O12 and BaNd2Ti5O14 phases for all the compositions. The increasing x value (0.02 ≤ x ≤ 0.1) in ((Ba1−x Sr x )4(Sm0.4Nd0.6)28/3Ti18O54...

Altered expression profiles of microRNA families during de-etiolation of maize and rice leaves

Background MicroRNAs (miRNAs) are highly conserved small non-coding RNAs that play important regulatory roles in plants. Although many miRNA families are sequentially and functionally conserved across plant kingdoms (Dezulian et al. in Genome Biol 13, 2005), they still differ in many aspects such as family size, average length, genomic loci etc. (Unver et al. in Int J Plant...

Identifying Multi-Dimensional Co-Clusters in Tensors Based on Hyperplane Detection in Singular Vector Spaces

Co-clustering, often called biclustering for two-dimensional data, has found many applications, such as gene expression data analysis and text mining. Nowadays, a variety of multi-dimensional arrays (tensors) frequently occur in data analysis tasks, and co-clustering techniques play a key role in dealing with such datasets. Co-clusters represent coherent patterns and exhibit...

Enhanced energy storage properties of Bi0.5Li0.5TiO3 modified Sr0.1Bi0.45Na0.45TiO3 based ceramics

Lead-free (1−x)Sr0.1Bi0.45Na0.45TiO3−xBi0.5Li0.5TiO3 (x = 0−0.4) ceramics were successfully prepared by a solid-state reaction technique. The effects of amount of Bi0.5Li0.5TiO3 on structure and electrical properties were examined. The X-ray diffraction (XRD) analysis revealed that all the investigated specimens have a perovskite structure. An obvious change in microstructure...

Modeling of Mechanical Stress Exerted by Cholesterol Crystallization on Atherosclerotic Plaques

Plaque rupture is the critical cause of cardiovascular thrombosis, but the detailed mechanisms are not fully understood. Recent studies have found abundant cholesterol crystals in ruptured plaques, and it has been proposed that the rapid expansion of cholesterol crystals in a limited space during crystallization may contribute to plaque rupture. To evaluate the effect of...

Glycoside Hydrolase MoGls2 Controls Asexual/Sexual Development, Cell Wall Integrity and Infectious Growth in the Rice Blast Fungus

N-linked glycosylation is a way of glycosylation for newly synthesized protein, which plays a key role in the maturation and transport of proteins. Glycoside hydrolases (GHs) are essential in this process, and are involved in processing of N-linked glycoproteins or degradation of carbohydrate structures. Here, we identified and characterized MoGls2 in Magnaporthe oryzae, which is...

Determination of sample size for a multi-class classifier based on single-nucleotide polymorphisms: a volume under the surface approach

Background Data on single-nucleotide polymorphisms (SNPs) have been found to be useful in predicting phenotypes ranging from an individual’s class membership to his/her risk of developing a disease. In multi-class classification scenarios, clinical samples are often limited due to cost constraints, making it necessary to determine the sample size needed to build an accurate...

High Level Expression and Purification of Recombinant Proteins from Escherichia coli with AK-TAG

Adenylate kinase (AK) from Escherichia coli was used as both solubility and affinity tag for recombinant protein production. When fused to the N-terminus of a target protein, an AK fusion protein could be expressed in soluble form and purified to near homogeneity in a single step from Blue-Sepherose via affinity elution with micromolar concentration of P1, P5- di (adenosine—5...

Evaluation of a Micro-Optical Coherence Tomography for the Corneal Endothelium in an Animal Model

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Proteases and Protease Inhibitors of Urinary Extracellular Vesicles in Diabetic Nephropathy

Diabetic nephropathy (DN) is one of the major complications of diabetes mellitus (DM), leads to chronic kidney disease (CKD), and, ultimately, is the main cause for end-stage kidney disease (ESKD). Beyond urinary albumin, no reliable biomarkers are available for accurate early diagnostics. Urinary extracellular vesicles (UEVs) have recently emerged as an interesting source of...

Immunohistochemical Study of Expression of Sohlh1 and Sohlh2 in Normal Adult Human Tissues

The expression pattern of Sohlh1 (spermatogenesis and oogenesis specific basic helix-loop-helix 1) and Sohlh2 in mice has been reported in previous studies. Sohlh1 and Sohlh2 are specifically expressed in spermatogonia, prespermatogonia in male mice and oocytes of primordial and primary follicles in female mice. In this report, we studied the expression pattern of Sohlh1 and...

MoMyb1 is required for asexual development and tissue-specific infection in the rice blast fungus Magnaporthe oryzae

Background The Myb super-family of proteins contain a group of functionally diverse transcriptional activators found in plant, animal and fungus. Myb proteins are involved in cell proliferation, differentiation and apoptosis, and have crucial roles in telomeres. The purpose of this study was to characterize the biological function of Myb1 protein in the rice blast fungus...

Association between Lipid Ratios and Insulin Resistance in a Chinese Population

Aim To explore the association of lipid ratios and triglyceride (TG) with insulin resistance (IR) in a Chinese population. We also provide the clinical utility of lipid ratios to identify men and women with IR. Methods This cross-sectional study included 614 men and 1055 women without diabetes. Insulin resistance was defined by homeostatic model assessment of IR > 2.69. Lipid...