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Contrasting patterns of nucleotide polymorphism suggest different selective regimes within different parts of the PgiC1 gene in Festuca ovina L.

Background Phosphoglucose isomerase (PGI, EC 5.3.1.9) is an essential metabolic enzyme in all eukaryotes. An earlier study of the PgiC1 gene, which encodes cytosolic PGI in the grass Festuca ovina L., revealed a marked difference in the levels of nucleotide polymorphism between the 5’ and 3’ portions of the gene. Methods In the present study, we characterized the sequence ...

Rheology of rock salt for salt tectonics modeling

Numerical modeling of salt tectonics is a rapidly evolving field; however, the constitutive equations to model long-term rock salt rheology in nature still remain controversial. Firstly, we built a database about the strain rate versus the differential stress through collecting the data from salt creep experiments at a range of temperatures (20–200 °C) in laboratories. The aim is ...

Wogonin as a targeted therapeutic agent for EBV (+) lymphoma cells involved in LMP1/NF-κB/miR-155/PU.1 pathway

Background Wogonin is an encouraging choice for clinical use owing to its potent anti-tumor and anti-inflammatory effects with the high safety profile. However, wogonin for targeted therapy of lymphoma was not well addressed. In this study, we focused on its anticancer effect alongside with the underlying mechanisms for targeted therapy in EBV-positive lymphoma. This will ...

CLASP2 is involved in the EMT and early progression after transurethral resection of the bladder tumor

Background Cytoplasmic linker-associated protein 2 (CLASP2) belongs to a family of microtubule plus-end tracking proteins that localizes to the distal ends of microtubules and regulate microtubule dynamics. We speculated that it might be involved in the epithelial-mesenchymal transition (EMT) and progression of bladder cancer (BC). Methods Western blotting and RT-PCR were used to ...

Astaxanthin attenuates total body irradiation-induced hematopoietic system injury in mice via inhibition of oxidative stress and apoptosis

Background The hematopoietic system is especially sensitive to total body irradiation (TBI), and myelosuppression is one of the major effects of TBI. Astaxanthin (ATX) is a powerful natural anti-oxidant with low toxicity. In this study, the effect of ATX on hematopoietic system injury after TBI was investigated. Methods Flow cytometry was used to detect the proportion of ...

Copy number variants of ABCF1, IL17REL, and FCGR3A are associated with the risk of gout

., Y.Y., X.W., X.X., Y.P., S.J., Y.W., Y.C., and Y.L. contributed reagents/materials/analysis tools. J.W., Z.D., H.Z., F.Z., and L.J. wrote the paper. Zheng Dong, Yuan Li, Jingru Zhou, Shuai Jiang, Yi ... , and indicate if changes were made. Zheng Dong and Yuan Li have contributed equally. Electronic supplementary material The online version of this article (doi:10.1007/s13238-017-0401-y) contains

Improved Data Preprocessing Algorithm for Time-Domain Induced Polarization Method with Digital Notch Filter

Time-domain induced polarization (TDIP) measurement is seriously affected by power line interference and other field noise. Moreover, existing TDIP instruments generally output only the apparent chargeability, without providing complete secondary field information. To increase the robustness of TDIP method against interference and obtain more detailed secondary field information, ...

A statistical approach for identifying differential distributions in single-cell RNA-seq experiments

The ability to quantify cellular heterogeneity is a major advantage of single-cell technologies. However, statistical methods often treat cellular heterogeneity as a nuisance. We present a novel method to characterize differences in expression in the presence of distinct expression states within and among biological conditions. We demonstrate that this framework can detect ...

Gold nanoparticle integrated with nanostructured carbon and quantum dots: synthesis and optical properties

Multilayer graphene shell encapsulated gold nanoparticle—quantum dot hybrids were derived by combining wet-chemical, thermal, and covalent chemistry approaches. Uniformly patterned gold nanoparticles on a silicon substrate were obtained via gold film deposition in an electroless method followed by a thermal dewetting process. The resulting gold nanoparticles were further surface ...

Embryology of two mycoheterotrophic orchid species, Gastrodia elata and Gastrodia nantoensis: ovule and embryo development

Background Gastrodia elata, a famous herbal medicine, has been received great attention on its treatments of headache, vertigo and epilepsy. Gastrodia nantoensis is a newly described species from central Taiwan with potential medicinal value. Gastrodia species are fully mycoheterotrophic orchids, and the courses of their seed development are more rapid as compared to the ...

Antimicrobial activity and stability of the d-amino acid substituted derivatives of antimicrobial peptide polybia-MPI

Antimicrobial peptide has the potential to be developed as new kind of antimicrobial agents with novel action mechanism. However, the susceptibility to protease is a drawback for potential peptides to be clinical used. d-amino acid substitution can be one way to increase the proteolytic stability of peptides. In the present study, we synthesized the d-lysines substituted analog ...

Comparison of the clinical effectiveness of 3D and 2D imaging systems for laparoscopic radical cystectomy with pelvic lymph node dissection

Objective To compare the clinical effectiveness of three-dimensional (3D) and two-dimensional (2D) laparoscopic imaging systems for radical cystectomy (RC) with pelvic lymph node dissection.

Identification and characterization of wheat drought-responsive MYB transcription factors involved in the regulation of cuticle biosynthesis

A plant cuticle forms a hydrophobic layer covering plant organs, and plays an important role in plant development and protection from environmental stresses. We examined epicuticular structure, composition, and a MYB-based regulatory network in two Australian wheat cultivars, RAC875 and Kukri, with contrasting cuticle appearance (glaucousness) and drought tolerance. Metabolomics ...

De novo assembly of transcriptome from next-generation sequencing data

(Nos. 31571310 and 31271409). COMPLIANCE WITH ETHICS GUIDELINES The authors Xuan Li, Yimeng Kong, Qiong-Yi Zhao, Yuan-Yuan Li and Pei Hao declare they have no conflict of interests. This article does

Effect of Post-Infiltration Soil Aeration at Different Growth Stages on Growth and Fruit Quality of Drip-Irrigated Potted Tomato Plants (Solanum lycopersicum)

Soil hydraulic principles suggest that post-infiltration hypoxic conditions would be induced in the plant root-zone for drip-irrigated tomato production in small pots filled with natural soil. No previous study specifically examined the response of tomato plants (Solanum lycopersicum) at different growth stages to low soil aeration under these conditions. A 2 × 6 factorial ...

Replication of the Shrimp Virus WSSV Depends on Glutamate-Driven Anaplerosis

Infection with the white spot syndrome virus (WSSV) induces a metabolic shift in shrimp that resembles the “Warburg effect” in mammalian cells. This effect is triggered via activation of the PI3K-Akt-mTOR pathway, and it is usually accompanied by the activation of other metabolic pathways that provide energy and direct the flow of carbon and nitrogen. Here we show that unlike the ...

Spin transport in undoped InGaAs/AlGaAs multiple quantum well studied via spin photocurrent excited by circularly polarized light

The spin diffusion and drift at different excitation wavelengths and different temperatures have been studied in undoped InGaAs/AlGaAs multiple quantum well (MQW). The spin polarization was created by optical spin orientation using circularly polarized light, and the reciprocal spin Hall effect was employed to measure the spin polarization current. We measured the ratio of the spin ...

Does Wrist Arthrodesis With Structural Iliac Crest Bone Graft After Wide Resection of Distal Radius Giant Cell Tumor Result in Satisfactory Function and Local Control?

Background Many techniques have been described for reconstruction after distal radius resection for giant cell tumor with none being clearly superior. The favored technique at our institution is total wrist fusion with autogenous nonvascularized structural iliac crest bone graft because it is structurally robust, avoids the complications associated with obtaining autologous fibula ...

Molecular Cloning and Functional Analysis of UV RESISTANCE LOCUS 8 (PeUVR8) from Populus euphratica

Ultraviolet-B (UV-B; 280–315 nm) light, which is an integral part of the solar radiation reaching the surface of the Earth, induces a broad range of physiological responses in plants. The UV RESISTANCE LOCUS 8 (UVR8) protein is the first and only light photoreceptor characterized to date that is specific for UV-B light and it regulates various aspects of plant growth and ...