BMC Research Notes

http://link.springer.com/journal/13104

List of Papers (Total 6,424)

Multiple immunofluorescence assay identifies upregulation of Active β-catenin in prostate cancer

ObjectivesTo apply a systems pathology-based approach to the quantification of nuclear Active β-catenin and human leukocyte antigen class I, and assess the biomarker involvement in a cohort of prostate tumor patients.ResultsThe systems pathology approach applied allows a precise quantification of the marker expression in the different cell compartments as well as the...

Applying quality improvement methods to neglected conditions: development of the South Asia Burn Registry (SABR)

ObjectiveSouth Asia has the highest mortality rate from burns in the world. Application of quality improvement methods to burn care can help identify health system gaps. Our overall aim is to introduce a sustainable hospital-based burn registry for resource-constrained settings to assess health outcomes of burn injury patients presenting to dedicated burn injury centers in South...

Early initiation of breastfeeding and colostrum feeding among mothers of children aged less than 24 months in Debre Tabor, northwest Ethiopia: a cross-sectional study

ObjectiveTo assess early initiation of breastfeeding and colostrum feeding practice among mothers of children aged less than 24 months in Debre Tabor.ResultTwo hundred ninety-seven (297) mothers of children aged less than 24 months participated which made the response rate of 98.1%. Among a total of 297 participants, early initiation of breastfeeding was practiced by 76.8% of...

Construction of a kiwifruit yeast two-hybrid cDNA library to identify host targets of the Pseudomonas syringae pv. actinidiae effector AvrPto5

ObjectiveBacterial canker is a destructive disease of kiwifruit caused by the Gram-negative bacterium Pseudomonas syringae pv. actinidiae (Psa). To understand the disease-causing mechanism of Psa, a kiwifruit yeast two-hybrid cDNA library was constructed to identify putative host targets of the Psa Type Three Secreted Effector AvrPto5.ResultsIn this study, we used the Mate...

Multivariate analysis of genomic variables, effective population size, and mutation rate

ObjectiveThe relationship between genomic variables (genome size, gene number, intron size, and intron number) and evolutionary forces has two implications. First, they help to unravel the mechanism underlying genome evolution. Second, they provide a solution to the debate over discrepancy between genome size variation and organismal complexity. Previously, a clear correlation...

High dose gabapentin does not alter tumor growth in mice but reduces arginase activity and increases superoxide dismutase, IL-6 and MCP-1 levels in Ehrlich ascites

ObjectivesThe purpose of this study was to evaluate the effect of gabapentin on Ehrlich tumor growth in Swiss mice, a highly aggressive and inflammatory tumor model. Mice were grouped into sets of 5 animals and treated from days 2 to 8 with gabapentin 30 mg/kg body weight (G30) or 100 mg/kg body weight (G100), or normal sterile saline (control).ResultsThe mice were euthanized on...

Influence of sexual maturation status on the relationship between body adiposity indicators and age: a cross-sectional study

ObjectivesTo determine the influence of sexual maturation status on adiposity indicators of children and adolescents.Results2412 individuals participated, 1285 (47.4%) males and 1408 (52.6%) females. There was moderate to weak correlation between age and adiposity indicators for both sexes. By analyzing the relationship between age and body fat indexes adjusted for Sexual...

Health professionals’ willingness to pay and associated factors for human papilloma virus vaccination to prevent cervical cancer at College of Medicine and Health Sciences University of Gondar, Northwest Ethiopia

ObjectivePreferences of health professionals’ for human papilloma virus vaccines was measured by monetary value through willingness to pay (WTP) approach that could help policy makers set priorities among alternative cervical cancer prevention methods in poor countries. The objective of this study was to assess the female health professionals’ willingness to accept and pay, and...

Textbooks authors, publishers, formats and costs in higher education

ObjectivesThere is little empirical data reported on retail prices of college textbooks beyond self-reported surveys and no published datasets. Textbooks, as an ancillary cost, can contribute to the overall rising cost of education which can impact upon students’ ability to succeed in Higher Education. This study sought to understand more about costs of college textbooks by...

Willingness to pay for community-based health insurance and associated factors among rural households of Bugna District, Northeast Ethiopia

ObjectiveCommunity based health insurance schemes are becoming recognized as powerful method to achieve universal health coverage and reducing the financial catastrophic shock of the community. Therefore, this study aimed to assess willingness to pay for community-based health insurance and associated factors among rural households of Bugna District, Ethiopia.ResultsA total of...

Satisfaction with job and family life, and association with smoking and alcohol drinking behaviors among young men in Malawi: analysis from a multiple indicator survey

ObjectiveThe objective of the present study was to investigate if satisfaction with job and family life has any connection with smoking and alcohol drinking behavior among young men in Malawi.ResultsResults of multivariable logistic regression analysis indicate that compared to men who were unemployed, those who were dissatisfied were 0.90 times less likely to be non-smokers [OR...

Myeloid differentiation primary response protein 88 (MyD88)-deficient dendritic cells exhibit a skewed cytokine response to BCG

ObjectiveMacrophages and dendritic cells (DCs) play key role in the recognition of mycobacterial infection and mounting of antimycobacterial immunity. In case of macrophages, recognition of BCG and other mycobacteria has been attributed predominantly to MyD88-dependent singling. Interestingly, in previous study with bone marrow-derived DCs, we have shown that BCG promotes the...

Prevalence and distribution of adhesins and the expression of fibronectin-binding protein (FnbA and FnbB) among Staphylococcus aureus isolates from Shahrekord Hospitals

ObjectiveOne of the most important causes of nosocomial infections is Staphylococcus aureus. The aim of this study was to determine the frequency of these genes and the rate of expression of these genes during nasal colonization among the personnel of Kashani and Hajar hospitals.ResultsIn this Analytical-descriptive study, 240 nasal swab specimens were collected from personnel of...

Glycemic control and correlates in a group of sub Saharan type 1 diabetes adolescents

ObjectivesThis study aims to describe the prevalence of glycemic control and related factors in a population of Sub-Saharan African T1D patients. We carried out a cross-sectional study including children and adolescents from seven different centers of the Changing Diabetes in Children (CDiC) program. All children enrolled in the program where recruited after parental consent...

Factors associated with diabetic foot among type 2 diabetes in Northern area of Saudi Arabia: a descriptive study

ObjectiveFoot complications are considered to be a devastating consequence of type 2 diabetes mellitus (T2DM), posing a major medical and economic burden. A prospective study was conducted at researchers’ area “Northern area of Saudi Arabia” to determine the factors associated with diabetic foot (DF) among T2DM patients. Identifying the extent of this problem and the associated...

Prevalence and molecular characterization of Salmonella enterica serovar Typhimurium from ice and beverages in Jakarta, Indonesia

ObjectiveThe presence of Salmonella enterica serovar Typhimurium becomes a concern in relation to the safety of drinking water and ice. We detected and enumerated the bacteria from ice and beverages collected from several areas in Jakarta. Most Probable Number (MPN) and multiplex PCR method were used. Three sets of primers were used rfbJ, fliC, and fljB. Two Multiplex PCR’s were...

Differential expression of RNA exosome subunits in the amphibian Lithobates catesbeianus during reproductive and non-reproductive periods

ObjectiveThe RNA exosome is an evolutionarily conserved 3′–5′ exoribonucleolytic protein complex involved in processing and degradation of different classes of nuclear and cytoplasmic RNAs, and, therefore, important for the posttranscriptional control of gene expression. Despite the extensive in vivo functional studies and the structural data on the RNA exosome, few studies have...

Analysis of reciprocating system real-time torque variation during root canal preparation

ObjectiveThis study monitored real-time torque variation of the WaveOne Gold (WOG) and Reciproc Blue (RB) during root canal preparation of mandibular molars. Thirty-six mandibular molars were prepared with WOG Primary 25.07 (WOGP, n = 36) and the RB R25 25.08 (RBR25, n = 36) for the mesial canals, whereas WOG Large 45.05 (WOGL, n = 18) and RB R40 40.06 (RBR40, n = 18) for the...

Level and factors associated with preference of institutional delivery among pregnant woman in Debre-tabor town, North West Ethiopia, 2017: a community based cross sectional study

ObjectiveMaternal mortality rates in Ethiopia remain highest in the world. Information with respect to factors that influence preference of institutional delivery among pregnant women are of relevance for designing intervention programs to reduce these deaths. This study aimed to determine level and factors affecting preference of institutional delivery among pregnant woman in...

A case series of venous thromboembolic disease in a semi-urban setting in Cameroon

ObjectiveOur goal was to study the clinical characteristics, risk factors and outcome of patients admitted for venous thromboembolism (VTE) in the medical unit of the Buea Regional Hospital, Cameroon between 1st July 2016 and 30th November 2017.ResultsTwenty-two patients were admitted for VTE. There were 12 (54.4%) men. The mean age was 54.9 ± 13.9 years (range: 31–77 years). The...

The effect of the health transformation plan on cesarean section in Iran: a systematic review of the literature

ObjectiveCesarean section (CS) is one of the most common surgical procedures in the world. In developed and developing countries, CS has grown significantly over the past decades. The Iranian Ministry of Health and Medical Education has developed a health transformation plan (HTP) in order to reduce CS rate and promote vaginal delivery. This study was conducted with the aim of...

Prevalence of dual contraceptive use and associated factors among HIV positive women at University of Gondar Hospital, Northwest Ethiopia

ObjectiveDual contraceptive is the use of male condom besides any modern contraceptive. It reduces parent to child transmission of HIV and other sexually transmitted infections between partners. The aim of this study was to investigate the prevalence and associated factors of dual contraceptive use among HIV positive women at University of Gondar Hospital, North West Ethiopia...

Healthcare workers knowledge and diagnostic practices: a need for dengue and chikungunya training in Moshi Municipality, Kilimanjaro Tanzania

ObjectiveDengue and chikungunya virus diseases are becoming an increasingly important global health threats and are continuously expanding their geographical range. The study aims to investigate knowledge and diagnostic practice of dengue and chikungunya fever among healthcare workers in Moshi Municipality.ResultsMost of healthcare workers heard of chikungunya and dengue 146 (71...

Simultaneous extraction of mRNA and microRNA from whole blood stabilized in tempus tubes

ObjectiveStudies of mRNA and miRNA expression profiling increasingly use stabilized whole blood. Commercial RNA extraction kits do not provide information about the simultaneous recovery of both mRNA and miRNA. This study evaluated yield, quality, integrity and representation of mRNA and miRNA from whole blood stabilized in Tempus tubes using three RNA extraction kits; two filter...

Study of biofilm formation and antibiotic resistance pattern of gram-negative Bacilli among the clinical isolates at BPKIHS, Dharan

ObjectivesGram-negative bacilli are the common causative agents for community-acquired, nosocomial and opportunistic infections. The recent upsurge of biofilm, as well as beta-lactamases producing strains, have synergistically led to the extensive dissemination of multi-drug resistant gram-negative bacilli. This study was carried out with an intention to detect the biofilm...