PLOS ONE

http://journals.plos.org/plosone/

List of Papers (Total 204,408)

The effect of the staining technique on morphological and morphometric parameters of boar sperm

Sperm morphology and morphometry are important parameters in predicting fertility. Sperm are considered to be normal if the shape and size of the head, midpiece and tail fall within the classification for a given species. It is important to select the appropriate technique for staining the semen of a given species, because, as many authors have pointed out, some methods work well...

Contribution of ERMES subunits to mature peroxisome abundance

Eukaryotic organelles share different components and establish physical contacts to communicate throughout the cell. One of the best-recognized examples of such interplay is the metabolic cooperation and crosstalk between mitochondria and peroxisomes, both organelles being functionally and physically connected and linked to the endoplasmic reticulum (ER). In Saccharomyces...

HIV prevalence, spatial distribution and risk factors for HIV infection in the Kenyan fishing communities of Lake Victoria

Introduction Global efforts to end HIV by 2030 focus on reducing and eventually eliminating new infections in priority populations. Identifying these populations and characterizing their vulnerability factors helps in guiding investment of scarce HIV prevention resources to achieve maximum impact. We sought to establish HIV prevalence, spatial distribution and risk factors for...

The unmet needs of family members of patients with progressive neurological disease in the Czech Republic

Background Caring for patients with a progressive neurological disease (PND) causes stress that may impact on the state of health as well as the quality of life of the caring family. Objective The aim of the study was to explore the unmet needs of the family members of patients with PND in advanced stages. Methods Grounded theory (constructivist approach) was used to...

The NRF2 transcriptional target NQO1 has low mRNA levels in TP53-mutated endometrial carcinomas

Background NRF2 is a major transcription factor regulating the expression of antioxidative/detoxifying enzymes, involved in oncogenic processes and drug resistance. We aimed to identify molecular alterations associated with NRF2 activation in endometrial carcinoma (EC). Methods Ninety patients treated (2012–2017) for localized/locally advanced EC were included in this study...

An open-source software analysis package for Microspheres with Ratiometric Barcode Lanthanide Encoding (MRBLEs)

Multiplexed bioassays, in which multiple analytes of interest are probed in parallel within a single small volume, have greatly accelerated the pace of biological discovery. Bead-based multiplexed bioassays have many technical advantages, including near solution-phase kinetics, small sample volume requirements, many within-assay replicates to reduce measurement error, and, for...

Online repetitive transcranial magnetic stimulation during working memory in younger and older adults: A randomized within-subject comparison

Working memory is the ability to perform mental operations on information that is stored in a flexible, limited capacity buffer. The ability to manipulate information in working memory is central to many aspects of human cognition, but also declines with healthy aging. Given the profound importance of such working memory manipulation abilities, there is a concerted effort towards...

Statistical analysis of bitcoin during explosive behavior periods

This paper develops the ability of the normal inverse Gaussian distribution (NIG) to fit the returns of bitcoin (BTC). As the first cryptocurrency created, the behavior of this new asset is characterized by great volatility. The lack of a proper definition or classification under existing theory exacerbates this property in such a way that explosive periods followed by a rapid...

Changes in ankle joint alignment after proximal fibular osteotomy

The purpose of this study was to assess the effect of proximal fibular osteotomy (PFO) on ankle joint. 49 patients or 53 lower limbs were included and followed-up with a minimum of one year in the study prospectively. Patients were evaluated radiographically and clinically. The questionnaires of the American Knee Society Score (KSS), the Ankle-Hindfoot Scale of the American...

“I was hungry and you gave me food”: Religiosity and attitudes toward redistribution

Current literature presents conflicting findings concerning the effect of religiosity on attitudes towards redistribution. This paper attempts to reconcile these findings by arguing that the belief and social behavior dimensions of religiosity affect support for redistribution via different mechanisms, and that these effects are moderated by state welfare generosity. Using...

Using a soil bacterial species balance index to estimate potato crop productivity

The development of ‘molecular-omic’ tools and computing analysis platforms have greatly enhanced our ability to assess the impacts of agricultural practices and crop management protocols on soil microbial diversity. However, biotic factors are rarely factored into agricultural management models. Today it is possible to identify specific microbiomes and define biotic components...

Exon prediction based on multiscale products of a genomic-inspired multiscale bilateral filtering

Multiscale signal processing techniques such as wavelet filtering have proved to be particularly successful in predicting exon sequences. Traditional wavelet predictor is domain filtering, and enforces exon features by weighting nucleotide values with coefficients. Such a measure performs linear filtering and is not suitable for preserving the short coding exons and the exon...

Unassigned diversity of planktonic foraminifera from environmental sequencing revealed as known but neglected species

Most research on extant planktonic foraminifera has been directed towards larger species (>0.150 mm) which can be easily manipulated, counted and yield enough calcite for geochemical analyses. This has drawn attention towards the macroperforate clade and created an impression of their numerical and ecological dominance. Drawing such conclusions from the study of such “giants” is...

Resilience as a predictive factor towards a healthy adjustment to grief after the loss of a child to cancer

Introduction Grief among bereaved parents is known to cause psychological distress and physical illness, but knowledge concerning factors that can contribute to health promotion after bereavement is scarce. Childhood cancer remains the most common non-accidental cause of death among children in Norway. The aim of the present study was to explore if resilience factors among cancer...

Impacts of event-specific air quality improvements on total hospital admissions and reduced systemic inflammation in COPD patients

There is limited evidence linking the impacts of reduced air pollution on hospital admissions. The potential biological mechanisms are still not completely understood. This study examined the effects of mitigated ambient pollution on hospital admissions and inflammatory biomarker levels in chronic obstructive pulmonary disease (COPD) COPD patients. Daily hospital admissions were...

Emotion analysis in children through facial emissivity of infrared thermal imaging

Physiological signals may be used as objective markers to identify emotions, which play relevant roles in social and daily life. To measure these signals, the use of contact-free techniques, such as Infrared Thermal Imaging (IRTI), is indispensable to individuals who have sensory sensitivity. The goal of this study is to propose an experimental design to analyze five emotions...

A cross-sectional survey on patient safety culture in secondary hospitals of Northeast China

Objectives This study aims to investigate patient safety culture in secondary hospitals of Heilongjiang, Northeast China, and explore the implications of patient safety culture and practices through the perspectives of various healthcare workers. Methods A cross-sectional survey using the Safety Attitude Questionnaire (SAQ) was conducted to ascertain the status of patient safety...

Comparative histopathologic and viral immunohistochemical studies on CeMV infection among Western Mediterranean, Northeast-Central, and Southwestern Atlantic cetaceans

Cetacean morbillivirus (CeMV) is a major natural cause of morbidity and mortality in cetaceans worldwide and results in epidemic and endemic fatalities. The pathogenesis of CeMV has not been fully elucidated, and questions remain regarding tissue tropism and the mechanisms of immunosuppression. We compared the histopathologic and viral immunohistochemical features in molecularly...

Reliance on Facebook for news and its influence on political engagement

This paper examines the link between reliance on Facebook for news, political knowledge, and political engagement in the Philippines. We tested five hypotheses using data gathered from an online survey of 978 Filipinos conducted from February 1 to March 31, 2016. Findings support the hypothesis that those who rely less on social media as a news source exhibit higher levels of...

Quantifying pine processionary moth defoliation in a pine-oak mixed forest using unmanned aerial systems and multispectral imagery

Pine processionary moth (PPM) feeds on conifer foliage and periodically result in outbreaks leading to large scale defoliation, causing decreased tree growth, vitality and tree reproduction capacity. Multispectral high-resolution imagery acquired from a UAS platform was successfully used to assess pest tree damage at the tree level in a pine-oak mixed forest. We generated point...

Rumination in bereaved parents: Psychometric evaluation of the Swedish version of the Utrecht Grief Rumination Scale (UGRS)

Background Bereaved parents may be at higher risk to develop persistent, severe and disabling grief, termed prolonged grief. Grief rumination, repetitive thinking about the causes and consequences of the loss, is a malleable cognitive process that maintains prolonged grief. Grief rumination can be measured with the Utrecht Grief Rumination Scale (UGRS). The present study aimed to...

Exploring the bacteriome in anthropophilic ticks: To investigate the vectors for diagnosis

Objective The aim of this study was to characterize the bacterial microbiome of hard ticks with affinity to bite humans in La Rioja (North of Spain). Methods A total of 88 adult ticks (22 Rhipicephalus sanguineus sensu lato, 27 Haemaphysalis punctata, 30 Dermacentor marginatus and 9 Ixodes ricinus) and 120 I. ricinus nymphs (CRETAV collection, La Rioja, Spain), representing the...

The effectiveness of colchicine combined with mitomycin C to prolong bleb function in trabeculectomy in rabbits

Purpose To investigate the potential of colchicine to improve bleb function after trabeculectomy. Methods To find the maximum usable colchicine concentration, an ocular irritation study was performed with the Draize test at concentrations of 0.001%, 0.01% and 0.1%. Additionally, the synergistic effect of topical colchicine instillation and MMC application to surgical site was...

Validation of the French version of the LEIPAD in community-dwelling people aged 80 years and above

Background Few studies have addressed health-related quality of life in community-dwelling individuals aged 80 years and above and very few self-assessment quality of life questionnaires have been formally validated in these populations. This study aimed to validate a French version of the LEIPAD, a self-administered questionnaire assessing the health-related quality of life of...