3 Biotech

http://link.springer.com/journal/13205

List of Papers (Total 681)

Application of silicon nanoparticles in agriculture

The beneficial effects of silicon and its role for plants are well established; however, the advantages of silicon nanoparticles over its bulk material are an area that is less explored. Silicon nanoparticles have distinctive physiological characteristics that allow them to enter plants and influence plant metabolic activities. The mesoporous nature of silicon nanoparticles also...

Site-directed mutation of β-galactosidase from Aspergillus candidus to reduce galactose inhibition in lactose hydrolysis

β-Galactosidase is widely used for hydrolysis of whey lactose. However, galactose inhibition has acted as a major constraint on the catalytic process. Thus, it is sensible to improve upon this defect in β-galactosidase through protein modification. To reduce the galactose inhibition of Aspergillus candidus β-galactosidase (LACB), four amino acid positions were selected for...

Analysis of the biodegradation of synthetic testosterone and 17α-ethynylestradiol using the edible mushroom Lentinula edodes

The mycelium of Lentinula edodes produces enzymes which may degrade xenobiotics including steroid hormones. The aim of the study was to determine whether the mycelium from in vitro cultures of L. edodes are able to degrade endocrine disruptors such as testosterone and 17α-ethynylestradiol. To prove the possibility of xenobiotics degradation, cultures of L. edodes were cultivated...

Equilibrium modeling of selenium binding from aqueous solutions by Candida utilis ATCC 9950 yeasts

The study investigated the effectiveness of selenium binding from its salt solution by Candida utilis ATCC 9950 yeast biomass cultured on a medium prepared from the agro-food industry wastes, containing an available source of carbon and nitrogen. Selenium binding by C. utilis yeast strain after 48 h of culturing at 28 °C from aqueous solutions with the addition of 30 mg Se/L...

Complete genome sequence of Rhodothermaceae bacterium RA with cellulolytic and xylanolytic activities

Rhodothermaceae bacterium RA is a halo-thermophile isolated from a saline hot spring. Previously, the genome of this bacterium was sequenced using a HiSeq 2500 platform culminating in 91 contigs. In this report, we report on the resequencing of its complete genome using a PacBio RSII platform. The genome has a GC content of 68.3%, is 4,653,222 bp in size, and encodes 3711 genes...

Futuristic biosensors for cardiac health care: an artificial intelligence approach

Biosensor-based devices are pioneering in the modern biomedical applications and will be the future of cardiac health care. The coupling of artificial intelligence (AI) for cardiac monitoring-based biosensors for the point of care (POC) diagnostics is prominently reviewed here. This review deciphers the most significant machine-learning algorithms for the futuristic biosensors...

In silico analysis of the α-amylase family GH57: eventual subfamilies reflecting enzyme specificities

Glycoside hydrolases (GHs) have been classified in the CAZy database into 153 GH families. Currently, there might be four α-amylase families: the main family GH13, the family GH57 with related GH119 and, eventually, also GH126. The family GH57 was established in 1996 as the second and smaller α-amylase family. In addition to α-amylase, it contains 4-α-glucanotransferase, α-glucan...

Recent advances and future prospects of iron oxide nanoparticles in biomedicine and diagnostics

Superparamagnetic iron oxide nanoparticles (SPIONs) are considered as chemically inert materials and, therefore, being extensively applied in the areas of imaging, targeting, drug delivery and biosensors. Their unique properties such as low toxicity, biocompatibility, potent magnetic and catalytic behavior and superior role in multifunctional modalities have epitomized them as an...

Changes in antioxidant and biochemical activities in castor oil-coated Capsicum annuum L. during postharvest storage

This study, for the first time, evaluates the efficiency of castor oil when used as an external coating on Capsicum annuum L., to increase postharvest storage-life at 4 ± 1 °C. The castor oil-coated fruits were successfully stored for 36 days, while the non-coated fruits could only sustain for 18 days. Throughout the storage period (at 9-day intervals), different antioxidants and...

Sustainability assessment of biogas production from buffalo grass and dung: biogas purification and bio-fertilizer

Biomass from wetland aquatic grass and buffalo grass can be exploited for biogas production, because this substrate is plenteous and does not compete with food production. In this study, the grass substrate was physically pretreated by boiling with different retention time to increase its biodegradability and was examined in batch mode. Boiling pretreatment suggested that 100 °C...

Whole-genome resequencing and transcriptomic analysis of genes regulating anthocyanin biosynthesis in black rice plants

Anthocyanins are involved in many diverse functions in rice, but their benefits have yet to be clearly demonstrated. Our objective in this study was to identify anthocyanin-related genes in black rice plants. We identified anthocyanin-related genes in black rice plants using a combination of whole-genome resequencing, RNA-sequencing (RNA-seq), microarray experiments, and reverse...

The production of UL16-binding protein 1 targeted pigs using CRISPR technology

Two sgRNAs were designed to target the region of exon 2 of the pULBP1 gene by microinjection. The co-injection of modified Cas9-D10A nickase with a pair of sgRNAs into the zygote’s cytoplasm easily and efficiently generated biallelic modification of the pULBP1 gene in one step. Five out of nine F0 generation piglets showed insertions or deletions in the targeting site of the...

Silicon bioavailability in exocarp of Cucumis sativus Linn.

Scanning electron microscopy (SEM) and electron probe micro-analyzer (EPMA) techniques have been used to detect the silicon bioavailability in the exocarp of warty cucumber surface. Warts appear at the time of anthesis and are remnant part of spines/trichomes which on further fruit maturation abscised from the exocarp. Results of EPMA and phytolith analysis clearly revealed that...

Modified microplate method for rapid and efficient estimation of siderophore produced by bacteria

In this study, siderophore production by various bacteria amongst the plant-growth-promoting rhizobacteria was quantified by a rapid and efficient method. In total, 23 siderophore-producing bacterial isolates/strains were taken to estimate their siderophore-producing ability by the standard method (chrome azurol sulphonate assay) as well as 96 well microplate method. Production...

Expression of STK11 gene and its promoter activity in MCF control and cancer cells

Serine/threonine kinase gene (STK11) is identified as tumor suppressor gene whose mutation can lead to Peutz–Jeghers syndrome (PJS). STK11 is emerging as a multifunctional protein, it activates 14 different AMP-activated protein kinase (AMPK) family members, important in the regulation of cell polarity, cell cycle arrest, energy and hemostasis. Present study was designed to...

The persistence and performance of phosphate-solubilizing Gluconacetobacter liquefaciens qzr14 in a cucumber soil

The persistence and performance of plant growth-promoting microorganisms (PGPMs) in soil are considered critical features for effectiveness, yet they are poorly understood. Here, we investigated the colonization and activity of a new PGPM, phosphate-solubilizing Gluconacetobacter liquefaciens qzr14, in a pot culture experiment using cucumber as test crop for 20 days. The number...

Allelic variation at high-molecular weight and low-molecular weight glutenin subunit genes in Moroccan bread wheat and durum wheat cultivars

Glutenin is a major protein fraction contributing to the functional properties of gluten and dough. The glutenin constitutes 30–40% of the protein in wheat flour and about half of that in gluten. It is essential to identify correct glutenin alleles and to improve wheat quality by selecting alleles that exert favorable effects. Moroccan wheat cultivars are unique in West Asia and...

Concurrent production and relative quantification of vasicinone from in vivo and in vitro plant parts of Malabar nut (Adhatoda vasica Nees)

The present study documents a simultaneous production and comparative assessment of extracted vasicinone from in vivo (leaves and stems) and in vitro (leaves, stems and calli) plant parts of Adhatoda vasica Nees, a well-known medicinal plant. High-performance thin layer chromatography (HPTLC) analysis of the above-mentioned plant parts, collected at their 60-day-old growth stage...

Characterization, drug loading and antibacterial activity of nanohydroxyapatite/polycaprolactone (nHA/PCL) electrospun membrane

Considering the important factor of bioactive nanohydoxyapatite (nHA) to enhance osteoconductivity or bone-bonding capacity, nHA was incorporated into an electrospun polycaprolactone (PCL) membrane using electrospinning techniques. The viscosity of the PCL and nHA/PCL with different concentrations of nHA was measured and the morphology of the electrospun membranes was compared...

Role of Cys73 in the thermostability of farnesyl diphosphate synthase from Geobacillus stearothermophilus

Farnesyl diphosphate synthase (FPPase) is an enzyme that catalyzes the condensation between one molecule of dimethylallyl diphosphate (DMAPP) and two molecules of isopentenyl diphosphate (IPP) to produce farnesyl diphosphate (FPP). FPP is an important precursor in the isoprenoid synthesis pathway. In this study, the crystal structure of FPPase from Geobacillus stearothermophilus...

Identification and characterization of the Chinese giant salamander (Andrias davidianus) miRNAs by deep sequencing and predication of their targets

MicroRNAs (miRNAs) are non-coding small RNA of approximately 22 nt in length. They are considered as key regulators for gene expression at the post-transcriptional level and play an important role in the regulation of many fundamental biological processes. Andrias davidianus, as one of the largest amphibian on earth, may represent the transitional type of animal from aquatic to...