Dynamic Infrared Thermography of Nanoheaters Embedded in Skin-Equivalent Phantoms

Journal of Nanomaterials, Jan 2018

Nanoheaters are promising tools for localized photothermal therapy (PTT) of malignant cells. The anisotropic AuNPs present tunable surface plasmon resonances (SPR) with ideal NIR optical response to be applied as theranostic agents. To this purpose, nanoparticles with branches are suitable because of the electromagnetic field concentrated at their vertices. We standardized a protocol to synthesize multibranched gold nanoparticles (MB-AuNPs) by the seed-growth method and found a size-seed dependence tunability on the hierarchy of branching. Once the optical response is evaluated, we tested the temporal stability as nanoheaters of the MB-AuNPs immersed in skin-equivalent phantoms by dynamic infrared thermography (DIRT). The most suited sample presents a concentration of  MB-AuNPs/mL showing good thermal stability with = 4.5°C, during 3 cycles of 10 min at 785 nm laser irradiation with power of 0.15 W. According to these results, the MB-AuNPs are suitable nanoheaters to be tested for PTT in more complex models.

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Dynamic Infrared Thermography of Nanoheaters Embedded in Skin-Equivalent Phantoms

Hindawi Journal of Nanomaterials Volume 2018 Dynamic Infrared Thermography of Nanoheaters Embedded in Skin-Equivalent Phantoms K. A. López-Varela 2 N. Cayetano-Castro 1 E. S. Kolosovas-Machuca 0 F. J. González F. S. Chiwo 0 J. L. Rodríguez-López 2 Leszek A. Dobrzan´ski 0 Coordinacio ́n para la Innovacio ́n y la Aplicacio ́n de la Ciencia y la Tecnolog ́ıa, Universidad Auto ́noma de San Luis Potos ́ı , San Luis Potos ́ı, SLP , Mexico 1 Nanoscience, Micro and Nanotechnology Center, National Polytechnic Institute , Av. Luis Enrique Erro s/n, Zacatenco, 07738 Mexico City , Mexico 2 Divisio ́n de Materiales Avanzados, Instituto Potosino de Investigacio ́n Cient ́ıfica y Tecnolo ́gica , A.C., Camino Presa San Jose ́ 2055, Lomas 4 Secc., 78216 San Luis Potos ́ı, SLP , Mexico Nanoheaters are promising tools for localized photothermal therapy (PTT) of malignant cells. The anisotropic AuNPs present tunable surface plasmon resonances (SPR) with ideal NIR optical response to be applied as theranostic agents. To this purpose, nanoparticles with branches are suitable because of the electromagnetic field concentrated at their vertices. We standardized a protocol to synthesize multibranched gold nanoparticles (MB-AuNPs) by the seed-growth method and found a size-seed dependence tunability on the hierarchy of branching. Once the optical response is evaluated, we tested the temporal stability as nanoheaters of the MB-AuNPs immersed in skin-equivalent phantoms by dynamic infrared thermography (DIRT). The most suited sample presents a concentration of 5.2 × 108 MB-AuNPs/mL showing good thermal stability with Δ = 4.5∘C, during 3 cycles of 10 min at 785 nm laser irradiation with power of 0.15 W. According to these results, the MB-AuNPs are suitable nanoheaters to be tested for PTT in more complex models. 1. Introduction The last twenty years’ research reports on nanostructured materials clearly indicate their potential to develop new technologies for different specialized areas. The extensive investigation work in shape controlled synthesis of metal nanoparticles (NPs) has allowed the achievement of structures with complicated geometric forms and the use of nontoxic chemicals for their synthesis. Gold plays a particular and special role in this area of size and shape controlled synthesis, and because of their properties, those NPs are studied for technological applications in fields such as renewable energies, catalysis, medicine, and photonics. Because of the optical and low-reactive properties of gold nanoparticles (AuNPs), they have been considered for medical applications as theranostic agents, which means that they can be simultaneously used for drug delivery [ 1 ], medical imaging [ 2–5 ], localized photothermal therapy (PTT) [6], and biological sensing [ 7, 8 ]. Nowadays, for some specific theranostic applications AuNPs are designed with anisotropic shapes, characteristic that tunes their surface plasmon resonance (SPR) from the visible to the near (NIR) and middle infrared (Mid-IR) regions of the spectra, also presenting local concentration of electromagnetic fields on the vertices [ 9 ]. The most common anisotropic AuNPs studied and used have been the gold nanorods (AuNRs) and stellated or spiky AuNPs, here termed multibranched gold nanoparticles (MB-AuNPs). The fact that NIR absorbance of MB-AuNPs fits the so-called therapeutic window present in the human tissue (700–1200 nm) [ 10 ], a spectral region where maximum penetration of light occurs due to the minimal absorption of blood components, such as hemoglobin and water [ 11 ], is important for these applications. With the goal to apply these gold nanoheaters on the PTT of cancer (localized heating for killing cancer cells) [ 12 ], we have to understand their thermal response under controlled conditions. Thermal response has been evaluated by dynamic thermography technique, as infrared imaging tool for realtime recording of the temperature increases (Δ ). Since the evaluation of nanosystems in real tissue samples presents many out-of-control factors, such as the complexity in the formation of protein corona on the nanoparticles and their interaction with the physiological cellular environment [ 13, 14 ], and besides the lack of the reproducibility of results due to the difficulty in finding identical specimens, we decided the use of gel phantoms as skin-equivalent model [ 12, 15– 17 ] which emulates the dielectric properties of the human body surface. Previous reports had evaluated the thermal response of AuNRs [ 18 ] in agar phantoms with intralipid (fat emulsion) as scattered agent [ 19 ] and suggested to be effective for tumor irradiation of 10 mm depth from the illuminated tissue surface. We use for the accurate analysis of MBAuNPs nanoheaters an optimal agar phantom model, with permittivity close to that of the human skin in the 60 GHz band [ 20 ]. The skin-equivalent phantom uses polyethylene powder (PEP) to decrease the real and imaginary parts of the permittivity. In this report we use the organic molecule HEPES, 2-[4(2-hydroxyethyl)-1-piperazinyl]ethanesulfonic acid, as shape directing agent. HEPES is one of the good buffers used in cell culture [ 21 ] and is also used as reducing and shape directing agent [ 22, 23 ] in the synthesis of nanoparticles by wet-chemical [ 24–26 ] and hydrothermal methods [27]. The shape promoted by HEPES in nanoparticle formation is mostly multibranched, varying from few to several peaks. Previously reported seeded-growth synthesis protocol, in which the HEPES act as directing agent and hydroxylamine (NH2OH⋅HCl) [ 28–30 ], is used as reducing agent [ 31 ] over the seeds surface; the hydroxylamine has the role of avoiding new nucleation events during the peak growth stage. It has been also reported that hydroxylamine influences or promotes the growth of stellated gold nanoparticles [ 32 ]. Thus, we start from this method that involves two molecules, both with capping and direct-shaping properties. The function of the HEPES and hydroxylamine during the growing stage of peaks has been determined by our experimental design on concentrations of the reactants. The seed-growth method allows obtaining good monodispersity in the final product shape and size. We also had found a size-seed dependence tunability on the hierarchy of branching. In this work, we evaluated the optical response of MBAuNPs by UV-Vis spectroscopy, and their morphology by electronic microscopy (SEM and HRTEM). Particle density was determined by inducted coupled plasma spectrometry (ICP), and the temporal stability as nanoheaters was tested in skin-equivalent phantoms by dynamic infrared thermography (DIRT). 2. Materials and Methods Deionized water was used for all experiments. The reagents used for NPs synthesis were obtained from Sigma-Aldrich: HAuCl4⋅3H2O, HEPES, NH2OH⋅HCl, and sodium citrate. For the preparation of skin-equivalent-phantom, polyethylene powder (PEP) was obtained from Baker, Agar from Sigma-Aldrich, and TX-151 from Oil Center, and all reagents were used as received without further treatment or purification. All glassware was cleaned with aqua regia (HCl : HNO3, 3 : 1) and rinsed with plenty of deionized water. The samples were characterized with UV-Vis absorption spectroscopy, acquired on a Cary 60 UV-Vis-NIR spectrophotometer at room temperature. ICP elemental quantification was performed on Variant 730-ES spectrometer. The electron micrographs were acquired in a transmission electron microscope JEOL 2100, and scanning electron micrographs in a FEIHelios Nanolab. The X-ray diffractograms were obtained in a Bruker DX-8 system at CuK source of wavelength 1.54056 A˚. The dynamic thermographs were taken at a distance of 0.3 m from the skin-equivalent phantom [ 15, 33 ], using a high-resolution infrared camera with thermal sensitivity better than 40 mK and a 480×360 focal plane array of VOx microbolometers (FLIR T600, FLIR Systems Inc., Wilsonville, OR). The phantoms were evaluated with a 785 nm laser diode with a spot size of 3.5 mm and optical power of 0.15 W. Temperature measurements of the skin-like phantom were taken with and without MB-AuNPs. Data was analyzed with FLIR-IR Research software. 2.1. Synthesis of Multibranched Gold Nanoparticles (MBAuNPs) Using a Seed-Growth Method. The 16 and 18 gold nanospheres (AuNSs) used as seeds were prepared by a reverse-modified Turkevich method [34] by citrate reduction of gold salt. Shortly, in a three-neck round-bottom flask with a condenser mounted, a citrate solution was brought to boiling by a heating mantle for 5 mins; then a gold solution of initial concentration of 25 mM was added, and after a light red ruby color appeared the system was left to react for half hour. For the synthesis of 16 nm AuNSs, the total concentration of sodium citrate and gold salt was 1.8 mM and 0.16 mM, respectively; and for 18 nm AuNSs were 0.75 mM and 0.25 mM. After the reaction was completed, the seeds solution was left to cool down at ambient temperature and used without modifications. For the MB-AuNPs synthesis we used three concentrations of HEPES buffer [25, 50, 75 mM] as solution. Briefly, under slowly stirring within an ice bath (∼4∘C) to 12.5 mL of HEPES 50 L of AuNSs (16 or 18 nm) was added, as well as 30, 60, or 120 L of hydroxylamine [0.1 M], and finally the dropwise addition of gold salt solution with rate of 0.375 mL/min to a f inal concentration of 0.07 or 0.13 mM. For the reaction to be complete it is necessary to leave the sample to rest 8 h in the fridge. The samples were washed three times by centrifugation and a drop of the concentrated colloid was deposited on an aluminum pin for SEM and on a lacey carbon copper grid for HRTEM. For UV-Vis measurements, the samples were analyzed as synthesized. The samples for ICP quantification were centrifuged and the NPs concentrated was dissolved in aqua regia. 2.2. Fabrication of Skin-Equivalent Phantom. For the fabrication of the control phantom a previously reported protocol was used, and the reagents concentration was maintained [ 20 ]. The procedure is as follows: a Buchner flask with deionized water and agar was heated until ∼80∘C under slow magnetic stirring and vacuum. Once the agar was dissolved and the boiling point was reached, the vacuum and heating were turned of f, the TX-151 was added and mixed gently with a stirring rod until its complete incorporation, and then the PEP was added to decrease the real and imaginary parts of the permittivity. After complete PEP incorporation the vacuum was turned on for a moment to avoid bubbles in the final phantom. Finally, the mix was placed into a petri box (ø = 55 mm) and left to solidify at ambient temperature. When phantoms with nanoparticles were prepared, a 1x concentrated of prewashed MB-AuNPs colloid was added and completely incorporated before TX-151 addition. For comparison, other phantoms with AuNSs were prepared, at the same NPs concentration of MB-AuNPs. 3. Results 3.1. Multibranched Gold Nanoparticles (MB-AuNPs). In order to prepare monodisperse MB-AuNPs, presynthesized gold single-crystalline and multitwinned nanoparticles are used as seeds for the growth of peaks [ 28, 29 ], which grow along preferential crystallographic directions of the metallic core [35]. According to previous reports, the capping agents [ 36 ] have preferential adsorption by specific crystalline faces of the metal seeds [ 37 ]; and this promotes an accelerated growth rate along specific crystallographic directions [ 7, 32, 38–40 ]. The AuNSs, obtained by the reverse Turkevich method, were monodispersed in size (see Figures 1 and SI-1); by ICP spectroscopy was determined the concentration of 2.39 × 1011 and 3.66 × 1011 NPs/mL for 16 and 18 nm AuNSs, respectively. We will see later that the structural characteristics of the AuNSs will influence the growth process of peaks; as can be seen in Figure 1(a), the 16-AuNSs present more symmetrical shape and monocrystalline structure, being different from 18AuNSs samples (Figure 1(a)) that show multitwinned structure and nonspherical multifaceted shapes. During the stage of peaks formation, the promotion of second-order branching (Figure 3) is up to the seed structure [ 41 ] and the stabilizer agent protection [ 7 ] at the nanoparticle surface, observed in the MB-AuNPs synthesized from 18 nm nanospheres (18 MBAuNPs), with 0.13 mM of gold salt. Also, we observed that the growth mechanism of the MB-AuNPs is influenced by the chemical characteristics of the reagents present in the growth solution and by its molar ratio with respect to gold ions. Therefore, both hydroxylamine and HEPES are dual agents, with reducing and shape capabilities. Manipulation of the role played by each reactive is made through experimental conditions such as temperature (e.g., to slow the HEPES reducing action) and concentration of hydroxylamine (using the necessary to reduce the gold ions). Within the molar rates used in this work, the hydroxylamine governs the reaction kinetics and the f inal shape of the peaks and the HEPES modulate the peaks growth. A simple way to know if the HEPES has been or not contributing as reducing agent is by means of UV-Vis spectroscopy (see Figure SI-2), since the nitrogen species from degraded HEPES can be detected by its absorption at ∼346 nm [ 22 ]. It is important to mention that, in the reaction where no hydroxylamine is used, the color changes of the colloid take place slowly, evidencing that HEPES is a slow reductor. Increasing the concentration of HEPES (from 25 to 50 mM), the growth of narrow peaks is promoted while the cores remain small. Then the addition of hydroxylamine is necessary to modulate the kinetics without excess of HEPES and will help to obtain better definition on the peaks and a monodisperse size and shape sample (see Figure SI-3). From the mechanism reduction of Au3+ by hydroxylamine proposed by Minati et al. [ 42 ], the molar ratio of Au3+ : NH2OH (1 : 3) was used to synthesize the sample with 16 nm nanospheres (16 MB-AuNPs), in order to avoid the intervention of HEPES reducing action (see Figure SI-2). We observed that, when hydroxylamine is present and increases its molar rate, the change of colors gets faster indicating the necessary use of a mild reducing agent (hydroxylamine) simultaneously with a directing shape agent (HEPES) for better anisotropic growth. We also have determined that an excess of hydroxylamine promotes the second-order branching in 18 MB-AuNPs, which was synthesized with molar rate of Au3+ : NH2OH of 1 : 6 (see Figure SI-4). It had been reported that the size of the NPs used as seeds inf luences the shape of f inal products [ 30, 43 ]. In our results we observed that from 16-AuNSs, MB-AuNPs of 80 nm of diameter can be obtained, synthesized with 0.07 mM of gold salt (16 MB-AuNPs, see Figure SI-5), and the colloid presents a maximum absorbance at 728 nm (Figure 2). On the other hand, dendritic growth is only perceptible in 18 MB-AuNPs (Figure 3), even if the concentration of gold ions is increased in the samples synthesized from 16-AuNSs (see Figure SI-6). The increase in gold ions leads to bigger cores and smaller length of peaks, due to an addition of gold atoms between peaks for minimization of energy, instead of the growth of longer peaks [ 44, 45 ]. At this stage we have shown that both sets of samples (16 and 18 MB-AuNPs) present good optical response for being used as nanoheaters. The next criteria for choosing a sample is the critical size for being used in an in vitro or in vivo environment. Thus, we decided the use of 16 MBAuNPs for being tested as nanoheaters; however, the optical response of 18 MB-AuNPs in the 830 nm (Figure 3) is suitable for applications in nanostructured system for SERS [ 46 ]. 3.2. Thermal Response of MB-AuNPs. Infrared (IR) imaging is based on the fact that any object at a temperature above absolute zero (−273∘C) will emit IR radiation, even if only weakly. T he human body has a low thermal emittance, radiating in a wavelength range that starts at around 3 m and peaks in the vicinity of 10 m and trails off from this point into the extreme IR and, negligibly, beyond it. The emissivity of human skin has a constant value between wavelengths of 2 and 14 m of 0.98 ± 0.01 for black skin and 0.97 ± 0.02 for white skin [ 47 ]; thus, human skin has a known and almost invariant emissivity in this wavelength region that makes IR imaging an ideal procedure to evaluate surface temperature of the human body [ 48, 49 ]. Because of the above arguments, the skin-equivalent phantoms with 0.62±0.01 reported emittance [17] is a well suited model for IR thermal evaluation. The thermal response of 16-AuNSs and 16 MB-AuNPs was evaluated inside the skin-equivalent phantoms by dynamic thermography. The samples were irradiated at 0.15 W power laser by 3 cycles of 10 mins and recorded with an IR camera, and the thermographs shown at Figure 4 were extracted at 555 s using the FLIR-RIR software, which allows the analysis of single points, line profiles, and averages of circle/rectangular areas. The plot in Figure 4(a) shows data from a line profile analyzed at different times, and it clearly can be observed that not only the incident laser area has been heated but also there is a radial propagation by diffusion of the generated heat; thus there is energy transmitted to the adjacent regions due to the immersed NPs. This effect is also observed in the control phantom analysis, but over a smaller area and with more uniform temperature as can be seen in Figure SI-7. Figure 4(b) reports the information on how fast the system absorbs the energy, achieving constant temperature at early irradiation times (60 s). This was also performed for evidence the continuity of the thermal response of immersed 16 MBAuNPs in the phantom as a function of time. The plot in Figure 5 corresponds to a dynamic thermal analysis, and from it we can appreciate the difference in the temperature reached by the three phantoms: the largest 21.0 20.5 20.0 0 s 7 s 313 s Line Profile (Li1) 555 s 600 s 780 s 0 El1 Ar1 200 Laser On Laser Off 0 200 Control 16-AuNSs 16 -"-AuNPs increase of localized temperature is measured in the sample prepared with 16 MB-AuNPs, compared to the phantom with 16-AuNSs and the one without the addition of NPs, used as a control. A red-shift of the LSPR is present for anisotropic MBAuNPs compared with spheres (see Figure 1), and a substantial increase in the heating efficiency with respect the sphere is expected. In our results, the maximum temperature increment in 16 MB-AuNPs phantom was achieved in 60 s and maintained during all the laser irradiation. This temperature increment is Δ = 4.5∘C and when the light source is turned off, a fast decay is observed. The comparison between the AuNSs and MB-AuNPs at the same concentration shows the evident increase of thermal response due to the anisotropic shape of MB-AuNPs and the localized SPR’s synergy, because the MB-AuNPs absorbs closer to the wavelength of laser irradiation. In the case of the phantom with 16-AuNSs, the increment in temperature can be described for the excitation of hot-spots created in the interaction among spherical NPs [ 50, 51 ]. The fact that there were not physical changes observed in the phantoms under laser irradiation, as leaking or deformation, even after the three cycles evaluation is important to mention. 4. Discussion We have shown that the control on the width, the length, the number of peaks, and the degree of dendritic growth gives rise to an easy tuning of the plasmon resonance spectra [ 6 ] and their corresponding higher transduction of light into heat, and therefore gold nanoheaters are good candidates for IR absorption for efficient photothermal therapy of malignant cancer cells. The structural characteristics of the AuNSs influenced the degree of ramification of the nanoparticle tips, resulting in second-order branches for MB-AuNPs synthesized from 18AuNSs, which present crystalline defects promoting directed anisotropy. From 16-AuNSs we could obtain monodisperse samples. After analyzing the impact of HEPES and hydroxylamine on the final tips shape, we estimated that the optimal molar ratio of hydroxylamine : Au ions is 3 : 1, where this amount is enough to avoid the intervention of HEPES in the reduction. Even if an excess of hydroxylamine may result in a better definition of tips, it can also cause the agglomeration of MB-AuNPs, due to the resulting high ionic force of the solution, that could not be used for clinical applications, and the same argument is valid for higher concentration of HEPES (75 mM). On the other side, the increase on the molar concentration of Au ions (and necessary hydroxylamine) leads to bigger cores and smaller length of tips; thus it shifts the SPR to lower wavelengths making them not practical for PTT. Regarding the dynamic thermography results for 16 MBAuNPs, we found that not only the incident laser area is being heated, but also there is a radial propagation of the released heat, which means there is transmission of energy around the adjacent region of the spot due to the immersed NPs. Therefore, the addition of MB-AuNPs results in the production of more efficient centers for scattering and reemission of energy. The temperature increment achieved by the nanoheaters in this work (Δ ∼ 5∘C) is good enough for effective and minimally harmful gold nanoparticles based photothermal therapy techniques [ 52 ]. This temperature increment from multibranched gold nanoparticles immersed in a phantom gel (as a model approach for the real environment in bioapplications) satisfies biomedical requirement for the treatment of superficial diseases (mycoses, fungal infections, and many different types of skin cancer) [ 53 ]. 5. Conclusions A synthesis method that uses nonaggressive chemicals for obtaining multibranched gold nanoparticles and secondary growth branching by tuning the seed size is presented. Analysis of the UV-Vis spectra and SEM micrographs allows us to understand the role of each reactant in the final shape of the nanoparticles. The HEPES molecule mainly grow tips from seeds with a random order; and the hydroxylamine can act as a directing shape agent, but this role only follows after its reduction role has been f inished in the reaction. A faster depletion of the reactants (by higher concentration of hydroxylamine) during the grow reaction promotes the growth of larger tips with second-order branches. We have determined that the synthesized multibranched gold nanoparticles increase the temperature in a localized area irradiated with a 785 nm laser. The temperature increment recorded was Δ = 4.5∘C. The 16 MB-AuNPs embedded in the phantom gel is a system that rapidly absorbs the proper incident energy, achieving a plateau of constant temperature within 60 s which is recovered during at least three different cycles. This evaluation indicates MB-AuNPs as an interesting system for being tested as nanoheater in biological models. Furthermore, the evaluation of their photothermal behavior in cultured cells is necessary for their direct application in medicine. We also propose to carry on the test with an irradiance lower than the ANSI regulation. Due to the experimental conditions reached in our phantoms, we expect that the transduction of light into thermal energy will be more effective, due to the limitation of MB-AuNPs reshaping. Conflicts of Interest Acknowledgments The authors declare that they have no conflicts of interest. The authors acknowledge Ph.D. CONACYT scholarship number 375630 and institutional support from IPICYT. The authors acknowledge Dra. Gladys Judith Labrada-Delgado for SEM and Dr. Hector Gabriel Silva-Pereyra for HRTEM analysis, as well as the infrastructure of the National Laboratory for Nanoscience and Nanotechnology (LINAN) at IPICYT. This work has been supported by CONACYTMexico Grants 106437 and 216315. F. J. Gonza´lez would like to acknowledge support from Project 32 of “Centro Mexicano de Innovacio´n en Energ´ıa Solar” and by the National Laboratory Program from CONACYT through the Terahertz Science and Technology National Lab (LANCYTT). Supplementary Materials Figure SI-1. Histograms of diameters and frequency distribution data of populations of (a) 200 AuNSs for 16-AuNSs and (b) 100 for 18-AuNSs. Figure SI-2. The increase of the molar concentration of HEPES promotes strong anisotropic formation of the MB-AuNPs. Figure SI-3. Scanning electron microscopy micrograph of 16 MB-AuNPs; the monodispersity and nonagglomeration of the NPs can be observed. Figure SI-4. Diagram of the concentrations of reactants used for the samples analyzed. Figure SI-5. Histograms of diameters and frequency distribution data of populations of 50 16 MB-AuNPs. Figure SI-6. SEM micrographs of MBAuNPs synthesized in a medium of HEPES [50 mM], with a molar ratio Au3+/NH2OH of 1 : 3, with (a) 16-AuNSs and (b)(c) 18-AuNSs. Figure SI-7. Thermography of skin-equivalent phantom used as control. (Supplementary Materials) Journal of Volume 20183 Hindawi www.hindawi.com Hindawi Hindawi www.hindawi.com International Journal of Polymer Science Advances in Physical Chemistry Condensed Matter Physics Advances in Chemistry Hindawi www.hindawi.com International Journal of Analytical Chemistry Hindawi www.hindawi.com Journal of Chemistry Hindawi www.hindawi.com International Journal of Biomaterials Advances in High Energy Physics Journal of Nanotechnology Hindawi www.hindawi.com Hindawi www.hindawi.com Hindawi Corrosion Hindawi www.hindawi.com Journal of [1] T. Lajunen , L. 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K. A. López-Varela, N. Cayetano-Castro, E. S. Kolosovas-Machuca, F. J. González, F. S. Chiwo, J. L. Rodríguez-López. Dynamic Infrared Thermography of Nanoheaters Embedded in Skin-Equivalent Phantoms, Journal of Nanomaterials, 2018, DOI: 10.1155/2018/3847348