Memórias do Instituto Oswaldo Cruz, Feb 2019
In the liver of one Macacus rhesus injected by BEAUREPAIRE ARAGÂO with blood of one benign case of yellow fever and in which he described (1) symptoms and typical lesions similar to those obtained by STOKES, BAUER and HUDSON in M. rhesus injected with the African virus, it has been found (2) changes in the nuclei analogous to those refered to in herpes zoster, symptomatic herpes, varicella and virus III infection of rabbits, and described either under the name of "acidophilic intranuclear inclusions" (LIPSCHÜTZ, GOODPASTURE), either as "oxychromatic degeneration" (LAUDA and LUGER). Similar changes inthe nuclei of liver cells were afterwards found by me in 13 M. rhesus and 2 M. cynomolgus injected with the Brazilian virus of yellow fever recovered independently by BEAUREPAIRE ARAGÂO (3) and A. MARQUES DA CUNHA and J. MUNIZ (4) from two benign cases of yellow fever; one of these monkeys was directly injected with the blood from the patient; two were injected with Aedes aegypti after having fed upon the blood of yellow fever patients or experimentally infected M. rhesus; the others were monkeys used in an unbroken series of passages of the virus through M. rhesus, and perhaps this accounts for notable differences found in the histopathology of the liver from case to case, as under natural conditions the virus never passes directly from man to man. The degree in which the oxychromatic degeneration occurs is in no way dependent upon the degree in which fatty and necrobiotic changes are found; in one instance it was the only change present in the liver, being there particularly intense. The acidophilic intranuclear inclusions (oxychromatic degeneration) were not present in several M. rhesus not inoculated and dying of tuberculosis or obscure causes; notwithstanding in these livers, fatty infiltration and degeneration associated to necrobiotic changes were found. Some stages ("butterfly" and "entamoeba-like" intranuclear figures, s. coloured Plate figs. g, and h) are rather frequent and by its characteristic shape facilitate the recognition of oxychromatic degeneration in sections made by a rapid technique (small pieces of liver fixed in folmaldehyde heated to 60ºC-thirty minutes, frozen sections, hematoxylin, eosin, absolute alcohol, phenol-xylol-creosote, xylol, balsam) having no peculiar importance besides this. Since the fatty degeneration and infiltration, necrosis and necrobiosis of the cells present considerable variations in degree from case to case in an unbroken series of passages of the virus through M. rhesus, lacking in two animals the liver of which was able to reproduce typical symptoms and lesions in the following passage, the acidophilic intranuclear inclusions (oxychromatic degeneration), as a rule, were found with much more regularity...
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C. Magarinos Torres. Alterações nucleares das cellulas do figado nas infecções de Macacus Rhesus e M. Cynomolgus pelo virus da febre amarella, Memórias do Instituto Oswaldo Cruz, 55-61, DOI: 10.1590/S0074-02761928000400003