Tick saliva and its role in pathogen transmission
Wiener klinische Wochenschrift
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Tick saliva and its role in pathogen transmission
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Patricia A. Nuttall
First Online: 06 May 2019
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Tick saliva is a complex mixture of peptidic and non-peptidic molecules that aid engorgement. The composition of tick saliva changes as feeding progresses and the tick counters the dynamic host response. Ixodid ticks such as Ixodes ricinus, the most important tick species in Europe, transmit numerous pathogens that cause debilitating diseases, e.g. Lyme borreliosis and tick-borne encephalitis. Tick-borne pathogens are transmitted in tick saliva during blood feeding; however, saliva is not simply a medium enabling pathogen transfer. Instead, tick-borne pathogens exploit saliva-induced modulation of host responses to promote their transmission and infection, so-called saliva-assisted transmission (SAT). Characterization of the saliva factors that facilitate SAT is an active area of current research. Besides providing new insights into how tick-borne pathogens survive in nature, the research is opening new avenues for vaccine development.
KeywordsIxodes ricinus Vaccine Saliva-assisted transmission Tick-borne encephalitis virus Borrelia burgdorferi
Ticks are arthropods related to spiders and scorpions. Nearly 900 tick species are recognized of which 702 are ixodid ticks (family, Ixodidae) while 193 are argasid ticks (family, Argasidae) and a single species exists in the Nuttalliellidae . All ticks go through a life cycle of egg, larva, nymph, and adult (female or male). Each postembryonic stage generally requires a blood meal before moulting to the next stage . During blood-feeding, ticks acquire infections they may subsequently transmit when feeding again. When this occurs, ticks act as vectors of potential pathogens of humans and other animals . In fact, ticks are believed to transmit the greatest variety of infectious agents of any blood-feeding vector. Notable diseases of humans caused by tick-borne pathogens include Lyme borreliosis, tick-borne encephalitis (TBE), tularemia, babesiosis, rickettsiosis, and human granulocytic anaplasmosis; however, tick-borne infectious agents (whether virus, bacterium, or protozoan) typically circulate in nature in a tick-vertebrate host-tick cycle without any apparent adverse effect on either the tick vector or the vertebrate host, be it reptile, bird, or mammal . Hence, humans are usually oblivious to their presence making them hard to detect unless expensive monitoring procedures are implemented.
Tick-borne infectious agents are transmitted in the saliva of ticks. Importantly, the saliva of infected ticks is not simply a watery medium carrying pathogens but a complex mixture of hundreds of different molecules (Table 1). These saliva constituents help ticks obtain their blood meal while maintaining homeostasis. Increasing evidence reveals that tick-borne infectious agents exploit the activity of saliva molecules to promote their transmission, so-called saliva-assisted transmission (SAT). This means of facilitated transmission occurs both when infected ticks feed on a susceptible vertebrate host and when uninfected ticks feed on an infected vertebrate host. Ticks may even facilitate pathogen transmission via exosomes, extracellular vesicles in tick saliva . In this review, the properties of tick saliva components and evidence of SAT are considered with particular focus on Ixodes ricinus (the wood or sheep tick), the most common tick species in northern and central Europe.
Composition of tick saliva
Excess water from host bloodmeal
Na+, Cl− (excess ions from host bloodmeal)
Adenosine, prostaglandins, endocannabinoids, microRNAs
Variegins, hyalomins, madanins
Chitinases, mucins, ixostatins, cystatins, defensins, glycine-rich, hyaluronidases, Kunitz-type proteins, lipocalins, metalloproteases
Immunoglobulins, haptoglobin, transferrin
May contain microRNA, peptides, proteins
Properties of tick saliva
Tick saliva is secreted from the relatively large and complex salivary glands of ticks during blood feeding. Besides water, saliva comprises a rich mixture of peptidic and non-peptidic molecules derived from the blood meal or synthesized by the salivary glands (Table 1). The volume and composition of saliva changes as feeding progresses, reflecting the uptake of blood (greatest during the final 24–48 h of engorgement) and dynamics of the host response. In large ixodid tick species, the total volume of saliva secreted during 10 days of engorgement may exceed 1 ml .
At least seven “functions” can be attributed to tick saliva: water balance, gasket and holdfast, control of host responses, dynamics, individuality, mate guarding, and SAT . Given that ixodid ticks increase their body mass 10-fold to 200-fold during engorgement, excretion of water and ions extracted from the blood meal is essential to maintain tick homoeostasis . Ensuring attachment is maintained during the long feeding period of ixodid ticks, and that nothing leaks out from the feeding site, is the role of the cement plug formed by polymerization of glycine-rich proteins secreted after initial attachment . The attachment process severs tissues including nerves, causing pain and provoking host hemostatic (vasoconstriction, blood platelet aggregation, and fibrin clot formation), inflammatory, and immune responses [9, 10]. Most of the constituents of tick saliva function to counter these host responses. Such bioactive tick saliva molecules include analgesic bradykinin inhibitors, antihemostatic prostaglandins, vasoconstriction modulators, platelet activation and aggregation inhibitors, anticoagulants, anti-inflammatory proteins, immunomodulators and wound healing inhibitors [11, 12, 13, 14, 15, 16]. As the host responses are dynamic (changing during the course of tick attachment, preparation of the feeding pool, and engorgement) so too is the saliva, changing in composition to meet the different needs of attachment, and slow and rapid feeding phases, and to counter the dynamic host responses [17, 18, 19]. Examination of single salivary glands of adult female I. ricinus confirmed expression of different clusters of genes at different times of feeding but also revealed differences between individual ticks . As feeding ticks often cluster together on a host, they have the potential to pool their molecular individuality, which may help them feed . Sharing of saliva resources has been demonstrated for male ticks as they “mate guard” their female mate . Although mate guarding is not an option for species such as I. ricinus that mate off the host, sharing saliva resources is a possibility for all tick species in SAT of tick-borne pathogens (Fig. 1).
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Major functions of tick saliva and their contribution to saliva-assisted transmission (SAT). Orange arrows indicate saliva functions contributing (solid line) or potentially contributing (dashed line) to SAT of tick-borne pathogens. Blue dashed line indicates potential for SAT to contribute to feeding success of infected ticks (modified from )
Saliva-assisted transmission (SAT)
The ability of saliva to enhance the infectivity of an arthropod-borne pathogen was first described for the sandfly, Lutzomyia longipalpis, and the protozoan parasite, Leishmania major . Follow-up of this discovery led to the finding that non-viremic transmission of a tick-borne virus between infected and uninfected ticks co-feeding on aviremic guinea pigs could be reproduced by inoculating the experimental guinea pigs with the virus mixed with an extract of uninfected tick salivary glands [23, 24]. The phenomenon was named saliva-activated transmission which was subsequently corrected to saliva-assisted transmission, defined as “the indirect promotion of arthropod-borne pathogen transmission via the actions of arthropod saliva molecules on the vertebrate host” . There is now extensive evidence that saliva of blood-feeding arthropods (mosquitoes, sandflies, ticks) affects the transmission of vector-borne viruses, bacteria, and protozoa in addition to simply acting as a transfer medium [12, 15, 26, 27, 28, 29, 30]. Considerable evidence relates to the immunomodulatory activities of tick saliva and, more recently, the dynamics of tick saliva-induced control of host responses (Fig. 1); however, tick cement may contribute to SAT (vide infra) as may the individuality of ticks each with its unique sialome .
This review focuses on pathogens transmitted by I. ricinus, the vector of numerous human pathogens, most notably TBE virus and certain species of the Borrelia burgdorferi sensu lato (s. l.) complex that cause Lyme borreliosis [31, 32]. Evidence of SAT is also considered involving the sympatric and parapatric species, I. persulcatus (found in parts of northern Europe and northern to north-eastern Asia), and the allopatric species of North America, I. scapularis [33, 34, 35, 36, 37].
Tick-borne encephalitis virus
At least 10 viruses (including subtypes and strains) transmitted by I. ricinus have been recorded in Europe . The most important of these as a human pathogen is TBE virus, a species belonging to the virus family, Flaviviridae . The TBE virus is distinguished into 3 subtypes: European, Siberian, and Far Eastern  and two additional subtypes, found in Siberia and the Himalayas, are proposed [41, 42]. In most of Europe, TBEV-Eu is the prevailing subtype, transmitted primarily by I. ricinus, and small rodents are the principal natural host . Despite ample evidence that tick saliva facilitates TBE virus transmission and infection, the mechanism and active saliva ingredients have not been identified for TBE virus or any other tick-borne virus (Table 2).
Saliva-assisted transmission of human pathogens transmitted by Ixodes ricinus
TBE virus (Far Eastern subtype)
Dermacentor marginatus adult SGE day 5
“Adjuvant” activity enhancing transmission to ticks
TBE virus (European subtype)
I. ricinus female SGE day 5
Enhanced transmission from guinea pigs to ticks
TBE virus (European subtype)
Co-feeding infected and uninfected I. ricinus
[45, 46, 47]
TBE virus (European subtype)
I. ricinus female saliva day 6
Enhanced replication in murine spleen dendritic cells ex vivo
Louping ill virus
Co-feeding infected and uninfected I. ricinus
Borrelia burgdorferi s.s.
Co-feeding infected and uninfected I. ricinus
Borrelia burgdorferi s.s.; B. lusitaniae
I. ricinus nymph SGE day 2–3
Enhanced spirochete load in mice
I. ricinus female SGE day 5
Enhanced infection of mice and feeding ticks
Borrelia burgdorferi s.s.
I. ricinus female saliva/SGE day 6
Enhanced transmission from mice to ticks
Borrelia burgdorferi s.s.
Facilitates transmission from ticks to miceb
Borrelia burgdorferi s.s.
Facilitates needle infection of immune mice
Borrelia burgdorferi s.s.; Borrelia garnii
Salp15 Iscap and Iric-1
In vitro protection against complement-mediated killing
Borrelia burgdorferi s.s.
In vitro suppression of borrelia-induced keratinocyte inflammation
Borrelia burgdorferi s.s.
Facilitates transmission from ticks to mice and from mice to ticksd
Borrelia burgdorferi s.s.; B. garinii
In vitro reduction of complement-mediated killing
In vitro protection against complement-mediated killing
Borrelia burgdorferi s.s.
Promotes late stage feeding and thereby facilitates tick to host transmission
Borrelia burgdorferi s.s.
Increases level of skin infection following syringe inoculation
Borrelia burgdorferi s.s.
Facilitates transmission from mice to ticks
Borrelia burgdorferi s.s.
In vitro inhibition of OspA and OspC-induced B cell proliferation
Promotes infection of mice by impairing inflammasome formation
I. ricinus female SGE day 5
Accelerated proliferation in mice
tHRF tick histamine release factor
aSalivary gland extract (SGE) or saliva collected at specified day of feeding, or specific saliva protein; recombinant proteins Iscap from I. scapularis and Iric from I. ricinus. Most studies involving specific saliva proteins are with I. scapularis-derived recombinant proteins.
bDirect evidence of Salp15-assisted transmission of B. burgdorferi has only been reported for I. scapularis 
cTick salivary lectin pathway inhibitor
dDirect evidence of TSLPI-assisted transmission of B. burgdorferi has only be reported for I. scapularis 
eDerived from I. scapularis
fB cell inhibitory protein from I. ricinus
Indirect evidence of SAT of TBE virus was first provided by experimental studies designed to test whether non-viremic transmission of Thogoto virus could be reproduced with TBE virus . Uninfected I. ricinus nymphs became infected with TBE virus when co-feeding (in separate retaining chambers) with infected I. ricinus adult ticks on guinea pigs that did not develop a patent viremia . Similar observations were reported when infected and uninfected ticks fed together; at the time, this was referred to as transptialonic transmission . Of major significance was the demonstration of non-viremic transmission with natural host species of TBE virus and I. ricinus (Table 2). Wild-caught field mice (Apodemus flavicollis and A. agrarius), bank voles (Myodes glareolus), pine voles (Pitymys subterraneus), hedgehogs (Erinaceaus europaeus) and pheasants (Phasianus colchicus), all of which had no evidence of prior exposure to TBE virus (absence of specific neutralizing antibodies) were infested with TBE virus-infected I. ricinus adult females and uninfected I. ricinus nymphs (in separate chambers). Although field mice showed undetectable or comparatively low levels of virus infection, they produced the greatest yield of infected ticks. By contrast, pine voles produced high levels of viremia and relatively few infected ticks because the animals died . The study provided the first evidence that TBE virus transmission from infected to uninfected ticks occurs during co-feeding on natural host species and is independent of the development of viremia. Indeed, models of TBE virus survival based on estimates of the basic reproduction number (R0) indicated that TBE virus cannot survive in nature without non-viremic/co-feeding transmission .
Co-feeding transmission takes advantage of the fact that ticks show a typical negative binomial distribution on their hosts: at any one time, a small number of individual animals are heavily infested with ticks whereas the majority of the host population are uninfested or support low numbers of ticks . Moreover, ticks are gregarious feeders; for example, >90% immature I. ricinus feed together on the ears of rodents or around the bill of birds . Minimization of the distance between co-feeding infected and uninfected ticks, as a result of feeding aggregation, facilitates non-viremic transmission [67, 71, 72]. Co-feeding/non-viremic transmission may also aid survival of TBE virus in natural foci of infection by amplifying low levels of transovarial transmission of the virus from one tick generation to the next . Survival is also aided by the ability of hosts immune to TBE virus to support co-feeding/non-viremic transmission, although at reduced levels compared with transmission involving non-immune natural host species . Given that non-viremic transmission is facilitated by SAT, the natural history of TBE virus demonstrates the crucial role tick saliva molecules play in maintaining a major human pathogen.
Evidence that TBE virus transmission is enhanced by factors associated with the salivary glands of uninfected ticks was first indicated experimentally when tick-infested guinea pigs were inoculated with a mixture of TBE virus and salivary gland extract (SGE) derived from uninfected partially fed adult Dermacentor marginatus (Table 2). The SGE was considered to act as an adjuvant, increasing the number of Dermacentor spp. nymphs that became infected while feeding on the non-viremic guinea pigs [43, 73]. When guinea pigs infested with uninfected Rhipicephalus appendiculatus nymphs were inoculated with a mixture of TBE virus and SGE, there was a 3-fold to 5‑fold increase in the number of infected ticks from animals inoculated with TBE virus and SGE from partially fed I. ricinus ticks compared with virus alone or virus plus SGE from unfed ticks . This was the first clear evidence that TBE virus transmission is enhanced by factors associated with the salivary glands of feeding ticks, and that these factors may explain the efficient transmission of TBE virus between infected and uninfected co-feeding ticks in natural non-viremic transmission.
Attempts to identify the salivary gland factor(s) assisting TBE virus or any other tick-borne virus have so far been unsuccessful. A study with Thogoto virus demonstrated similar SAT dynamics for saliva and SGE collected from the same individual uninfected ticks at different days of feeding . Maximum activity was observed with saliva at day 6 of feeding and days 6–8 for SGE. The similar dynamics of SAT activity are a strong indication that the SAT factor(s) is synthesized in the salivary glands during feeding and secreted into the skin feeding site in tick saliva. Interestingly, SAT of tick-borne viruses has only been demonstrated with arthropod species that are competent vectors. Thus, SGE of I. ricinus does not promote SAT of Thogoto virus (for which I. ricinus is not a competent vector) although SAT occurs with TBE virus [44, 75, 76]. This implies that the mechanism underlying SAT differs for different vector-virus associations.
Further studies using Thogoto virus provided additional insights into the mechanism of SAT of tick-borne viruses. To examine whether the SAT factor has a direct or an indirect effect on Thogoto virus, the time interval was varied between virus inoculation and injection of SGE into the same skin site of experimental guinea pigs. Similar levels of SAT were observed when virus + SGE were inoculated together compared with an interval between SGE injection followed by virus inoculation of 72 h or less; when the interval was 96 h the level of SAT was halved. In the converse experiment, SAT levels were similar when the interval between virus inoculation followed by SGE injection was 0 h, 24 h, or 48 h but was significantly reduced at higher intervals . Moreover, SAT is a localized effect rather than a generalized response in the host: when virus + SGE were inoculated into different skin sites of experimental guinea pigs, SAT was not observed. In addition, in vitro and in vivo titrations of Thogoto virus mixed with SGE showed no effect on infectivity . These observations indicate that SAT (at least for Thogoto virus) results from the effect of saliva in the feeding site rather than from a direct effect on the virus and the beneficial effect, whatever it may be, lasts for several days. They also suggest some dynamic variance: virus and SAT factor do not need to be delivered to the feeding site simultaneously to be effective. This window of activity may be significant as tick-borne virus transmission occurs within 24 h of tick attachment, and possibly within the first hour of feeding [78, 79, 80]. The onset of feeding enhances virus replication in the salivary glands [67, 81, 82]. Once initiated, tick-borne virus transmission may continue throughout the feeding period . Hence, tick-borne viruses may experience a broad spectrum of saliva-mediated activities in the skin site of tick feeding although SAT activity has only been reported with saliva/SGE collected at least 4 days after commencement of feeding (Table 2). In addition, the cement plug may act as a bolus of infection for both TBE virus and B. burgdorferi s. l. Removal of attached infected ticks while leaving the cement cone embedded in the skin resulted in infection with TBE virus within 1 h of attachment and after 20–22 h of attachment in the case of B. burgdorferi s. l. and I. persulcatus .
The SAT factor(s) promoting Thogoto virus transmission by its principal vector, R. appendiculatus, appears to be proteinaceous as activity was lost following protease treatment . It remains to be determined whether these features apply to SAT of other tick-borne viruses including TBE virus although it seems likely. Enhanced replication of TBE virus occurs in murine bone marrow dendritic cells treated with the feeding-induced saliva protein, sialostatin L2 from I. scapularis nymphs ; however, sialostatin L2 has not been characterized in the natural vector of TBE virus, I. ricinus. Enhanced virus replication and survival in dendritic cells treated with saliva of partially fed adult female I. ricinus is attributed to saliva-induced modulation of the pro-survival phosphatidylinositol 3 kinase (P13)/Akt signal transduction pathway by an as yet unknown mechanism [48, 86].
In contrast to tick-borne viruses, at least two SAT factors have been identified for mosquito-borne viruses. A saliva protein from Aedes aegypti, CLIPA3 protease, promotes replication and dissemination of dengue type 2 viruses in interferon knockout mice through cleavage of extracellular matrix proteins; resulting dermal liquefaction is thought to facilitate virus infection of skin immune cells . In a similar mouse-mosquito model, a 15 kD A. aegypti saliva protein, LTRIN, augmented pathogenesis of Zika virus by interfering with signalling through the lymphotoxin-β receptor . In cultures of human keratinocytes, a 34 kD A. aegypti saliva protein of unknown function enhanced dengue type 2 virus replication . Further studies are needed to determine whether this saliva protein promotes initial skin infection following mosquito-borne transmission of dengue virus. Progress in identifying SAT factors of mosquito-borne viruses will hopefully inspire research on the saliva molecules promoting transmission of TBE virus and other tick-borne viruses.
Borrelia burgdorferi sensu lato
The bacterial complex, Borrelia burgdorferi s. l., includes 18 species of which 3 species commonly infect humans causing Lyme borreliosis: B. burgdorferi sensu stricto (s.s.), B. afzelii, and B. garinii . As in the case of tick-borne viruses (vide supra), the first hint that saliva plays an enhancing role in the transmission of B. burgdorferi s. l. was in studies showing efficient non-systemic transmission of B. burgdorferi s.s. between infected nymphs and uninfected I. ricinus larvae co-feeding on uninfected laboratory mice . In subsequent studies, experimental mice were inoculated with a mixture of either B. burgdorferi s.s. (isolated from I. scapularis) or B. lusitaniae (isolated from I. ricinus) and SGE prepared from either uninfected I. scapularis or I. ricinus partially fed nymphs (Table 2). The SGE promoted infection of the mice; the effect was tick-spirochete species-specific . Similar results were observed using B. afzelii isolated from I. ricinus and SGE from partially fed adult female I. ricinus . Inoculation of mice with saliva mixed with B. burgdorferi s.s. (isolated from I. ricinus) showed the same enhancing effect as with SGE .
Studies on B. burgdorferi s. l. were the first to identify a tick-derived SAT factor, Salp15. This saliva protein was originally identified as a glycosylated 15 kD feeding-inducible protein (at a concentration of 1 µg/ml saliva) that inhibits CD4+ T cell activation, the first I. scapularis protein associated with the immunosuppressive activity of tick saliva . The Salp15 binds to the CD4 coreceptor of mammalian T cells, inhibiting subsequent receptor ligand-induced early cell signalling, which explains its immunosuppressive activity and specificity for CD4 T cells ; however, Salp15 also binds to the C‑type lectin receptor, dendritic cell-specific intercellular adhesion molecule-3-grabbing non-integrin (DC-SIGN, also known as CD209), inhibiting Toll-like receptor (TLR)-induced production of proinflammatory cytokines (including cytokines induced by B. burgdorferi) and dendritic cell-induced T cell activation . The activity of Salp15 appears long lasting, possibly due to Salp15-induced upregulation of CD73 (5′-ectonucleotidase) in regulatory T cells, which increases production of adenosine, a recognized immunosuppressant .
An initial indication that Salp15 might be involved in SAT was the selective enhancement of salp15 expression observed in I. scapularis nymphs infected with B. burgdorferi s.s. . Further studies showed that Salp15 protected B. burgdorferi s.s. from antibody-mediated killing by binding to the outer surface protein, OspC, of the spirochete. This lipoprotein is expressed when the spirochete infects the tick salivary glands and during the early stages of vertebrate host infection . Knockdown of salp15 in infected I. scapularis markedly reduced the spirochete load in mice on which the nymphs fed (levels of infection were similar in the salivary glands of engorged salp15-repressed and control nymphs). These observations were replicated using white-footed mice, Peromyscus leucopus (natural hosts of I. scapularis and B. burgdorferi s.s. in North America), even when the mice were immune to the spirochete. The gene knockdown experiments provide the first direct evidence that Salp15 promotes tick-borne transmission of B. burgdorferi . Protection of Salp15-immunized mice against I. scapularis-transmitted B. burgdorferi s.s. provides further supportive data .
Following the discovery of Salp15-assisted transmission of B. burgdorferi s.s. by I. scapularis, other Ixodes species were investigated. Orthologues of Salp15 were found in all B. burgdorferi s. l. vector species including 3 homologues in I. ricinus . One of these homologues (Salp15 Iric-1) shares 82% homology with the I. scapularis protein and is highly expressed at 3 days of feeding. Like Salp15 from I. scapularis, Salp15 Iric-1 binds to OspC. Mice antibody-positive for B. burgdorferi s.s. (strain N40) were more susceptible to infection with strain N40 preincubated with Salp15 Iric-1 compared with needle challenge with the untreated strain; however, Salp15 Iric-1 did not facilitate infection of B. afzelii-immune mice with B. afzelii .
Comparison of recombinant Salp15 derived from I. ricinus and I. scapularis revealed protection of serum-sensitive B. garinii and B. burgdorferi s.s. strains of intermediate sensitivity against complement-mediated killing by normal human serum. The effect was significantly stronger for Salp15 from I. ricinus compared with I. scapularis . Interestingly, Salp15 Iric-1 binding to B. burgdorferi s.s. OspC conferred protection against antibody-mediated killing whereas binding of Salp15 Iric-1 to OspC from B. garinii and B. afzelii was not protective even though binding affinities were similar . The I. ricinus Salp15 also shows an antialarmin effect on human primary keratinocytes in vitro, suppressing inflammation induced by B. burgdorferi s.s. infection .
The differential effects of Salp15 in relation to complement and antibody-mediated killing may help explain spirochete-vector species specificity . If these effects of Salp15 (and possibly other saliva proteins) occur when ticks feed on a natural host or a human, the ensuing inhibition of cutaneous innate immunity (including suppression of immune cell recruitment) will most likely promote B. burgdorferi transmission and infection of the host. Note the effects will also help the infected tick by suppressing the undesirable (from the tick’s point of view) host responses to infection by the pathogen.
Characterization of Salp15-assisted transmission of B. burgdorferi demonstrates the importance of classical (antibody-dependent) and alternative (antibody-independent) complement pathways in vertebrate host control of the spirochete; however, Ixodes vector species also interfere with the lectin (antibody-independent) complement cascade [58, 59]. The lectin pathway is activated when the pattern recognition molecules of the lectin pathway, ficolins and collectins (including mannan-binding lectin), bind to highly glycosylated pathogen-associated molecular patterns on the surface of pathogens . Tick salivary lectin pathway inhibitor (TSLPI) is a feeding-induced 8 kD saliva protein first identified in I. scapularis and initially designated P8 . Unlike Salp15, TSPLI does not bind to B. burgdorferi but instead interacts with the lectin pathway pattern recognition molecules, inhibiting complement activation and reducing complement-mediated lysis of B. burgdorferi s. l. . In vitro, TSLPI impairs complement-mediated chemotaxis and phagocytosis of B. burgdorferi s. l. by neutrophils. Heat-inactivated TSLPI antiserum substantially reduces the complement inhibitory activity of I. scapularis SGE in vitro, indicating that TSLPI is a dominant complement inhibitor in tick saliva. Heat-inactivated TSLPI antiserum also reduces the lectin pathway inhibitor activity of I. scapularis SGE, showing that native TSLPI is a major inhibitor of tick-borne B. burgdorferi-mediated complement activation through the lectin pathway. Compared with uninfected ticks, infection of I. scapularis nymphs with B. burgdorferi s.s. results in significantly higher TSLPI mRNA levels in salivary glands 24 h after tick attachment . The timing neatly coincides with tick-borne delivery of Borrelia into the feeding site. Knockdown of TSLPI in nymphs reduced the Borrelia load in infected ticks fed on uninfected mice for 72 h. Transmission of B. burgdorferi s.s. to uninfected mice by TSLPI knockdown nymphs resulted in a significantly lower spirochete load in skin 7 days postinfestation and reduced dissemination at 21 days. Similar effects were observed when infected nymphs were fed on mice which had been passively administered TSLPI antiserum . Acquisition of B. burgdorferi s.s. by I. scapularis larvae was impaired on infected TSLPI-immune mice and the Borrelia load was reduced in nymphs moulted from the larvae compared with the controls. Results of the TSLPI knockdown experiments provide direct evidence of TSLI-assisted transmission of B. burgdorferi s.s. by I. scapularis supported by abrogation of the effect in TSLPI-immune mice. As killing of Borrelia by the lectin complement pathway occurs in the absence of B. burgdorferi antibodies, the protective effect of TSLPI is most likely a critical factor in tick-borne B. burgdorferi infection of naïve humans.
Bioinformatic analysis of Ixodes species indicates TSLPI comprises a family of saliva proteins . An orthologue from I. ricinus, upregulated during feeding, inhibits the lectin complement pathway and protects B. burgdorferi s.s. and B. garinii from complement-mediated killing in vitro. Studies of the role of I. ricinus TSLPI in tick-borne transmission of B. burgdorferi have not been reported to date. Additionally, numerous inhibitors of the alternative complement pathway have been recognized in Ixodes ticks (including I. ricinus), the first of which was named Ixodes scapularis anti-complement (ISAC). The ISAC-related proteins (designated the IxAC family) target properdin, a positive activator of the alternative complement pathway . The family includes Salp20 (I. scapularis salivary protein 20) which provides partial protection of complement sensitive B. garinii against lysis by normal human serum in vitro . Although the IxAC family may aid complement-sensitive B. burgdorferi strains during the early stages of skin invasion in mammals, their role in tick-borne borrelia transmission has not been explored.
Histamine is an important tick deterrent . Not surprisingly, ticks have evolved saliva molecules that control host-derived histamine ; however, what is surprising is that certain tick species deliberately release histamine into the tick feeding site . The tick histamine release factor (tHRF) of I. scapularis appears to aid the late (rapid) phase of feeding and also to promote B. burgdorferi s.s. transmission . Expression of this feeding-induced 24 kD protein is enhanced in B. burgdorferi infected I. scapularis nymphs, knockdown of tHRF-impaired feeding and borrelia transmission. As tHRF is critical for I. scapularis feeding irrespective of B. burgdorferi infection, and preferentially expressed 48–72 h post-tick attachment (while B. burgdorferi transmission begins 36–48 h post-tick attachment), it seems likely that the effect on borrelia transmission is due to the ability of tHRF to promote engorgement rather than to a specific interaction as with Salp15 (vide supra). Indeed, it is thought that tHRF promotes engorgement by enhancing blood flow into the tick feeding site during the final 24 h of feeding when ~50% of the blood meal is taken up. Conceivably, the vasodilatory effect of histamine may aid dissemination of borrelia injected by the tick into the feeding site . As yet, an I. ricinus orthologue of I. scapularis tHRF has not been characterized.
Evidence that the cysteine protease, sialostatin L2, is a SAT factor was first observed following syringe inoculation of I. scapularis sialostatins and B. burgdorferi s.s. into the skin of mice. In the presence of sialostatin L2, the number of spirochetes in the skin increased almost 6‑fold, 4 days post-inoculation, whereas sialostatin L had no effect . Sialostatin L2 does not bind directly to spirochetes and appears not to affect B. burgdorferi s.s. growth in culture . In borrelia-infected murine dendritic cells, sialostatin L2 inhibited borrelia-induced chemokine production and Toll-like receptor signalling pathways [85, 104]. Thus, sialostatin L2 may assist B. burgdorferi by suppressing the pathogen-induced inflammatory response. An orthologue of sialostatin L2 has not been reported for I. ricinus although a putative orthologue has been recorded in I. persulcatus .
Most SAT factors are considered in relation to pathogen transmission from infected ticks to a susceptible vertebrate host ; however, some saliva proteins appear to aid in the acquisition of the pathogen by uninfected feeding ticks. One such protein is Salp25D, an antioxidant protein identified in both the salivary glands and midgut of I. scapularis . Knockdown of salivary gland Salp25D dramatically reduces acquisition of B. burgdorferi s.s. by feeding I. scapularis nymphs and larvae but does not affect spirochete transmission from infected nymphs to uninfected mice. Ticks failed to acquire B. burgdorferi when fed on mice immunized against Salp25D. In vitro, oxygen radicals produced by activated neutrophils reduce the viability of B. burgdorferi, whereas in the presence of recombinant Salp25D or adult I. scapularis saliva, viability was unaffected. Thus, it appears that Salp25D assists in transmission of B. burgdorferi from infected mice to uninfected ticks by protecting borrelia from the toxic products of neutrophils activated by tick feeding.
An 18 kD B cell inhibitory protein (BIP) identified in the salivary glands of I. ricinus, inhibits B lymphocyte proliferation induced by B. burgdorferi outer surface proteins, OspA and OspC . As these lipoproteins play essential roles in B. burgdorferi infection of the tick midgut and in tick-borne transmission to a vertebrate host, respectively, BIP may act as a localized SAT transmission factor facilitating both tick-borne transmission and tick acquisition of the spirochete.
A number of other saliva proteins, mainly from I. scapularis, affect B. burgdorferi transmission. These include subolesin [107, 108] and calreticulin . Their effect appears to result from their critical role in tick physiological processes rather than acting as SAT factors per se. Histone H4 isolated from I. ricinus SGE has a dissociating effect on human primary fibroblast and antimicrobial properties although it does not affect B. burgdorferi s.s. . These characteristics suggest that histone H4 plays a role in formation of the tick feeding pool within the skin and thereby may aid borrelia transmission.
Other human pathogens transmitted by Ixodes ricinus
Louping ill virus, a close relative of TBE virus, causes disease in sheep and red grouse (Lagopus scotica) in the UK, and is an occupational health risk for veterinarians, game keepers, and farm workers . The discovery that mountain hares (Lepus timidus) support non-viremic transmission of louping ill virus (even when the hares have antibodies to the virus), identified the reservoir host of this virus . This has led to large-scale culling of mountain hares on Scottish moorland managed for red grouse, which has given rise to considerable controversy .
The intracellular bacterium, Anaplasma phagocytophilum, has long been known as the etiological agent of tick-borne fever affecting ruminants and was only recognized relatively recently as the pathogen causing human granulocytic ehrlichiosis . Although studies have shown that sialostatin L2 from I. scapularis plays a role in transmission of A. phagocytophilum, the role of I. ricinus saliva proteins in A. phagocytophilum transmission, including orthologues of sialostatin L2, has not been reported (Table 2).
Bartonella spp. are linked with an increasing number of human diseases of which the most common are the multiple clinical symptoms associated with cat scratch disease, ocular infections, and endocarditis caused by B. henselae. Cat fleas are the principal vector of B. henselae; however, I. ricinus is also a vector of this intracellular bacterium although the epidemiological significance of tick-borne transmission is unknown . A serine protease inhibitor from I. ricinus (IrSPI) affects B. henselae salivary gland infection and tick feeding success . The role of IrSPI in transmission of B. henselae has not been determined.
The etiological agent of tularemia, Francisella tularensis, is an intracellular bacterium endemic in European rodent populations and hares (Lepus europaeus), and transmitted directly by contact and inhalation, and by ticks . Mice injected with a mixture of the live vaccine strain of F. tularensis and SGE supported accelerated proliferation of the bacterium in skin and other target organs (Table 2). As Th1-dependent cell-mediated immunity is critical for protection against infection with F. tularensis, the observed SGE-induced polarization to a Th2 cytokine profile most likely benefited the tularemic bacteria .
Just as sandfly saliva-leishmania was the forerunner to discoveries of the role of tick saliva in tick-borne pathogen transmission (vide supra), so vaccine development using sandfly saliva proteins to control leishmaniasis is pioneering a new approach to controlling arthropod-borne pathogens [116, 117]. For mosquito-borne pathogens, this has developed as far as a phase 1 clinical trial in humans of a vaccine comprising 4 mosquito-derived saliva antigens . Protection against lethal tick-borne challenge with TBE virus by immunization of mice with a tick cement protein, demonstrates the potential of this approach for controlling tick-borne pathogens . Tick saliva proteins that facilitate pathogen transmission have been identified as candidates for development of anti-tick vaccines [30, 120]. An alternative strategy is to target tick proteins that have a significant physiological role. For example, salivary gland aquaporins provide water transmembrane channels crucial for water homeostasis during blood feeding. Survival of I. ricinus larvae fed on rabbits immunized with a vaccine comprising recombinant I. ricinus aquaporin was significantly reduced . For leishmania vaccine development, the most promising results involve a dual pathogen plus vector vaccine design . Clearly there is much to do in the development of new, effective and efficient vaccines for controlling ticks and important major tick-borne infections. At least it is now realized that “saliva is mightier than the needle”—future vaccine development needs to include challenge with infected ticks and not the typical needle and syringe inoculation of the challenge pathogen [122, 123, 124].
Conflict of interest
P.A. Nuttall declares that she has no competing interests.
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Guglielmone AA, Robbins RG, Apanaskevich DA, et al. The Argasidae, Ixodidae and Nuttalliellidae (Acari: Ixodida) of the world: a list of valid species names. Zootaxa. 2010;2528:1–28.CrossRefGoogle Scholar
Apanaskevich DA, Oliver JH. Life cycles and natural history of ticks. In: Sonenshine DE, Roe RM, editors. Biology of ticks. 2nd ed. Oxford: Oxford University Press; 2013. pp. 59–73.Google Scholar
Kahl O. Hard ticks as vectors—some basic issues. Wien Klin Wochenschr. 2018;130:479–83.PubMedCrossRefGoogle Scholar
Rizzoli A, Silaghi C, Obiegala A, et al. Ixodes ricinus and its transmitted pathogens in urban and peri-urban areas in Europe: new hazards and relevance for public health. Front Public Health. 2014;2:1–26. https://doi.org/10.3389/fpubh.2014.00251/abstract.CrossRefGoogle Scholar
Hackenberg M, Kotsyfakis M. Exosome-mediated pathogen transmission by arthropod vectors. Trends Parasitol. 2018;34:549–52.PubMedCrossRefGoogle Scholar
Kaufman RW. Ticks: physiological aspects with implications for pathogen transmission. Ticks Tick Borne Dis. 2010;1:11–22.CrossRefGoogle Scholar
Nuttall PA. Wonders of tick saliva. Ticks Tick Borne Dis. 2019;10:470–81. https://doi.org/10.1016/j.ttbdis.2018.11.005.CrossRefPubMedGoogle Scholar
Suppan J, Engel B, Marchetti-Deschmann M, Nürnberger S. Tick attachment cement—reviewing the mysteries of a biological skin plug system. Biol Rev Camb Philos Soc. 2018;93:1056–76.PubMedCrossRefGoogle Scholar
Richter D, Matuschka F, Spielman A, Mahadevan L. How ticks get under your skin: insertion mechanics of the feeding apparatus of Ixodes ricinus ticks. Proc R Soc, B, Biol Sci. 2013; https://doi.org/10.1098/rspb.2013.1758.CrossRefGoogle Scholar
Ribeiro JMC. Role of saliva in blood-feeding by arthropods. Annu Rev Entomol. 1987;32:463–78. https://doi.org/10.1146/annurev.en.32.010187.002335.CrossRefPubMedGoogle Scholar
Francischetti IMB. Platelet aggregation inhibitors from hematophagous animals. Toxicon. 2010;56:1130–44.PubMedCrossRefGoogle Scholar
Fontaine A, Diouf I, Bakkali N, et al. Implication of haematophagous arthropod salivary proteins in host-vector interactions. Parasit Vectors. 2011;4(1):187.PubMedPubMedCentralCrossRefGoogle Scholar
Chmelar J, Calvo E, Pedra JHF, Francischetti IMB, Kotsyfakis M. Tick salivary secretion as a source of antihemostatics. J Proteomics. 2012;75:3842–54. http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=3383439&tool=pmcentrez&rendertype=abstract.PubMedPubMedCentralCrossRefGoogle Scholar
Kotál J, Langhansová H, Lieskovská J, et al. Modulation of host immunity by tick saliva. J Proteomics. 2015;128:58–68. http://www.sciencedirect.com/science/article/pii/S1874391915300610.PubMedPubMedCentralCrossRefGoogle Scholar
Šimo L, Kazimirova M, Richardson J, Bonnet SI. The essential role of tick salivary glands and saliva in tick feeding and pathogen transmission. Front Cell Infect Microbiol. 2017;7:281. https://doi.org/10.3389/fcimb.2017.00281/full.CrossRefPubMedPubMedCentralGoogle Scholar
Wikel SK. Tick-host-pathogen systems immunobiology: an interactive trio. Front Biosci. 2018;23:265–83. https://www.bioscience.org/2018/v23/af/4590/fulltext.php?bframe=PDF.CrossRefGoogle Scholar
Mudenda L, Pierlé SA, Turse JE, et al. Proteomics informed by transcriptomics identifies novel secreted proteins in Dermacentor andersoni saliva. Int J Parasitol. 2014;44:1029–37.PubMedCrossRefGoogle Scholar
De Castro MH, De Klerk D, Pienaar R, Rees DJG, Mans BJ. Sialotranscriptomics of Rhipicephalus zambeziensis reveals intricate expression profiles of secretory proteins and suggests tight temporal transcriptional regulation during blood-feeding. Parasit Vectors. 2017;10(1):1–20.CrossRefGoogle Scholar
Karim S, Ribeiro JMC. An insight into the sialome of the Lone Star tick, Amblyomma americanum, with a glimpse on its time dependent gene expression. PLoS ONE. 2015;10:e131292. https://doi.org/10.1371/journal.pone.0131292.CrossRefPubMedPubMedCentralGoogle Scholar
Perner J, Kropáčková S, Kopáček P, Ribeiro JMC. Sialome diversity of ticks revealed by RNAseq of single tick salivary glands. Plos Negl Trop Dis. 2018;12:e6410. https://doi.org/10.1371/journal.pntd.0006410.CrossRefPubMedPubMedCentralGoogle Scholar
Wang H, Paesen GC, Nuttall PA, Barbour AG. Male ticks help their mates to feed. Nature. 1998;391:753–4.PubMedCrossRefGoogle Scholar
Titus RG, Ribeiro JM. Salivary gland lysates from the sand fly Lutzomyia longipalpis enhance Leishmania infectivity. Science. 1988;239:1306–8.PubMedCrossRefGoogle Scholar
Jones LD, Davies CR, Steele GM, Nuttall PA. A novel mode of arbovirus transmission involving a nonviremic host. Science. 1987;237:775–7.PubMedCrossRefGoogle Scholar
Jones LD, Hodgson E, Nuttall PA. Enhancement of virus transmission by tick salivary glands. J Gen Virol. 1989;70:1895–8.PubMedCrossRefGoogle Scholar
Nuttall PA, Labuda M. Saliva-assisted transmission of tick-borne pathogens. In: Ticks: biology, disease and control. Cambridge: Cambridge University Press; 2008. pp. 205–19.CrossRefGoogle Scholar
Nuttall PA, Labuda M. Tick-host interactions: saliva-activated transmission. Parasitology. 2004;129:S177–89.PubMedCrossRefGoogle Scholar
Abdeladhim M, Kamhawi S, Valenzuela JG. What’s behind a sand fly bite? The profound effect of sand fly saliva on host hemostasis, inflammation and immunity. Infect Genet Evol. 2014;28:691–703. https://doi.org/10.1016/j.meegid.2014.07.028.CrossRefPubMedPubMedCentralGoogle Scholar
Pingen M, Schmid MA, Harris E, McKimmie CS. Mosquito biting modulates skin response to virus infection. Trends Parasitol. 2017;33:645–57. https://doi.org/10.1016/j.pt.2017.04.003.CrossRefPubMedGoogle Scholar
Bernard Q, Jaulhac B, Boulanger N. Smuggling across the border: how arthropod-borne pathogens evade and exploit the host defense system of the skin. J Invest Dermatol. 2013;134:1211–9.PubMedCrossRefGoogle Scholar
Liu XY, Bonnet SI. Hard tick factors implicated in pathogen transmission. Plos Negl Trop Dis. 2014;8:e2566.PubMedPubMedCentralCrossRefGoogle Scholar
Lindquist L, Vapalahti O. Tick-borne encephalitis. Lancet. 2008;371:1861–71.PubMedCrossRefGoogle Scholar
Radolf JD, Caimano MJ, Stevenson B, Hu LT. Of ticks, mice and men: understanding the dual-host lifestyle of Lyme disease spirochaetes. Nat Rev Microbiol. 2012;10:87–99.PubMedPubMedCentralCrossRefGoogle Scholar
Medlock JM, Hansford KM, Bormane A, et al. Driving forces for changes in geographical distribution of Ixodes ricinus ticks in Europe. Parasit Vectors. 2013;6:1.PubMedPubMedCentralCrossRefGoogle Scholar
Eisen RJ, Eisen L, Beard CB. County-scale distribution of Ixodes scapularis and Ixodes pacificus (Acari: Ixodidae) in the continental United States. J Med Entomol. 2016;53(2):349–86.PubMedPubMedCentralCrossRefGoogle Scholar
Jaenson TGT, Värv K, Fröjdman I, et al. First evidence of established populations of the taiga tick Ixodes persulcatus (Acari: Ixodidae) in Sweden. Parasit Vectors. 2016;9:377. https://doi.org/10.1186/s13071-016-1658-3.CrossRefPubMedPubMedCentralGoogle Scholar
Korenberg EI, Kovalevskii YV, Gorelova NB, Nefedova VV. Comparative analysis of the roles of Ixodes persulcatus and I. trianguliceps ticks in natural foci of ixodid tick-borne borrelioses in the Middle Urals, Russia. Ticks Tick Borne Dis. 2015;6:316–21.PubMedCrossRefGoogle Scholar
Leighton PA, Koffi JK, Pelcat Y, Lindsay LR, Ogden NH. Predicting the speed of tick invasion: an empirical model of range expansion for the Lyme disease vector Ixodes scapularis in Canada. J Appl Ecol. 2012;49:457–64.CrossRefGoogle Scholar
Hubálek Z, Rudolf I. Tick-borne viruses in Europe. Parasitol Res. 2012;111:9–36.PubMedCrossRefGoogle Scholar
Simmonds P, Becher P, Bukh J, et al. ICTV virus taxonomy profile: flaviviridae. J Gen Virol. 2017;98:2–3.PubMedPubMedCentralCrossRefGoogle Scholar
Ecker M, Allison SL, Meixner T, Heinz FX. Sequence analysis and genetic classification of tick-borne encephalitis viruses from Europe and Asia. J Gen Virol. 1999;80:179–85. https://doi.org/10.1099/0022-1317-80-1-179.CrossRefPubMedGoogle Scholar
Kovalev SY, Mukhacheva TA. Reconsidering the classification of tick-borne encephalitis virus within the Siberian subtype gives new insights into its evolutionary history. Infect Genet Evol. 2017;55:159–65.PubMedCrossRefGoogle Scholar
Dai X, Shang G, Lu S, Yang J, Xu J. A new subtype of eastern tick-borne encephalitis virus discovered in Qinghai-Tibet Plateau, China article. Emerg Microbes Infect. 2018;7:74. https://doi.org/10.1038/s41426-018-0081-6.CrossRefPubMedPubMedCentralGoogle Scholar
Alekseev AN, Chunikhin SP, Rukhkian MI, Stefutkina LF. The possible role of the salivary gland substrate in ixodid ticks as an adjuvant enhancing arbovirus transmission. Parazitologiia. 1991;1:28–31.Google Scholar
Labuda M, Jones LD, Williams T, Nuttall PA. Enhancement of tick-borne encephalitis virus transmission by tick salivary gland extracts. Med Vet Entomol. 1993;7:193–6.PubMedCrossRefGoogle Scholar
Labuda M, Jones LD, Williams T, Danielova V, Nuttall PA. Efficient transmission of tick-borne encephalitis virus between cofeeding ticks. J Med Entomol. 1993;30:295–9.PubMedCrossRefGoogle Scholar
Labuda M, Nuttall PA, Kožuch O, Elečková E, Williams T, Žuffová E, et al. Non-viraemic transmission of tick-borne encephalitis virus: a mechanism for arbovirus survival in nature. Experientia. 1993;49:802–5.PubMedCrossRefGoogle Scholar
Labuda M, Danielova V, Jones LD, Nuttall PA. Amplification of tick-borne encephalitis virus infection during co-feeding of ticks. Med Vet Entomol. 1993;7:339–42.PubMedCrossRefGoogle Scholar
Fialová A, Cimburek Z, Iezzi G, Kopecký J. Ixodes ricinus tick saliva modulates tick-borne encephalitis virus infection of dendritic cells. Microbes Infect. 2010;12:580–5.PubMedCrossRefGoogle Scholar
Jones LD, Gaunt M, Hails RS, et al. Transmission of louping ill virus between infected and uninfected ticks co-feeding on mountain hares. Med Vet Entomol. 1997;11(2):172–6.PubMedCrossRefGoogle Scholar
Gern L, Rais O. Efficient transmission of Borrelia burgdorferi between cofeeding Ixodes ricinus ticks (Acari: Ixodidae). J Med Entomol. 1996;33:189–92.PubMedCrossRefGoogle Scholar
Zeidner NS, Schneider BS, Nuncio MS, Gern L, Piesman J. Coinoculation of Borrelia spp. with tick salivary gland lysate enhances spirochete load in mice and Is tick species–specific. J Parasitol. 2002;88:1276–8. https://doi.org/10.1645/0022-3395%282002%29088%5B1276%3ACOBSWT%5D2.0.CO%3B2.CrossRefPubMedGoogle Scholar
Pechová J, Štěpánová G, Kovář L, Kopecký J. Tick salivary gland extract-activated transmission of Borrelia afzelii spirochaetes. Folia Parasitol (Praha). 2002;49:153–9.CrossRefGoogle Scholar
Horká H, Černá-Kýčková K, Skallová A, Kopecký J. Tick saliva affects both proliferation and distribution of Borrelia burgdorferi spirochetes in mouse organs and increases transmission of spirochetes to ticks. Int J Med Microbiol. 2009;299:373–80.PubMedCrossRefGoogle Scholar
Ramamoorthi N, Narasimhan S, Pal U, et al. The Lyme disease agent exploits a tick protein to infect the mammalian host. Nature. 2005;436:573–7.PubMedPubMedCentralCrossRefGoogle Scholar
Hovius JW, Schuijt TJ, de Groot KA, et al. Preferential protection of Borrelia burgdorferi sensu stricto by a Salp15 homologue in Ixodes ricinus saliva. J Infect Dis. 2008;198:1189–97.PubMedPubMedCentralCrossRefGoogle Scholar
Schuijt TJ, Hovius JWR, Van Burgel ND, Ramamoorthi N, Fikrig E, Van Dam AP. The tick salivary protein Salp15 inhibits the killing of serum-sensitive Borrelia burgdorferi sensu lato isolates. Infect Immun. 2008;76(7):2888–94.PubMedPubMedCentralCrossRefGoogle Scholar
Marchal C, Schramm F, Kern A, et al. Antialarmin effect of tick saliva during the transmission of Lyme disease. Infect Immun. 2011;79(2):774–85.PubMedCrossRefGoogle Scholar
Schuijt TJ, Coumou J, Narasimhan S, et al. A tick mannose-binding lectin inhibitor interferes with the vertebrate complement cascade to enhance transmission of the Lyme disease agent. Cell Host Microbe. 2011;10(2):136–46.PubMedPubMedCentralCrossRefGoogle Scholar
Wagemakers A, Coumou J, Schuijt TJ, et al. An Ixodes ricinus tick salivary lectin pathway inhibitor protects Borrelia burgdorferi sensu lato from human complement. Vector Borne Zoonotic Dis. 2016;16(4):223–8. https://doi.org/10.1089/vbz.2015.1901.CrossRefPubMedGoogle Scholar
Tyson K, Elkins C, Patterson H, Fikrig E, De Silva A. Biochemical and functional characterization of Salp20, an Ixodes scapularis tick salivary protein that inhibits the complement pathway. Insect Mol Biol. 2007;16(4):469–79.PubMedCrossRefGoogle Scholar
Dai J, Narasimhan S, Zhang L, Liu L, Wang P, Fikrig E. Tick histamine release factor is critical for Ixodes scapularis engorgement and transmission of the Lyme disease agent. Plos Pathog. 2010;6(11):e1001205. https://doi.org/10.1371/journal.ppat.1001205.CrossRefPubMedPubMedCentralGoogle Scholar
Kotsyfakis M, Horka H, Salat J, Andersen JF. The crystal structures of two salivary cystatins from the tick Ixodes scapularis and the effect of these inhibitors on the establishment of Borrelia burgdorferi infection in a murine model. Mol Microbiol. 2010;77(2):456–70.PubMedPubMedCentralCrossRefGoogle Scholar
Narasimhan S, Sukumaran B, Bozdogan U, et al. A tick antioxidant facilitates the Lyme disease agent’s successful migration from the mammalian host to the arthropod vector. Cell Host Microbe. 2007;2(1):7–18.PubMedPubMedCentralCrossRefGoogle Scholar
Hannier S, Liversidge J, Sternberg JM, Bowman AS. Characterization of the B‑cell inhibitory protein factor in Ixodes ricinus tick saliva: a potential role in enhanced Borrelia burgdoferi transmission. Immunology. 2004;113(3):401–8.PubMedPubMedCentralCrossRefGoogle Scholar
Wang X, Shaw DK, Sakhon OS, et al. The tick protein sialostatin L2 binds to annexin A2 and inhibits NLRC4-mediated inflammasome activation. Infect Immun. 2016;84(6):1796–805.PubMedPubMedCentralCrossRefGoogle Scholar
Kročová Z, Macela A, Hernychová L, Kroča M, Pechová J, Kopecký J. Tick salivary gland extract accelerates proliferation of Francisella tularensis in the host. J Parasitol. 2003;89(1):81–90. https://doi.org/10.1645/0022-3395%282003%29089%5B0014%3ATSGEAP%5D2.0.CO%3B2.CrossRefGoogle Scholar
Alekseev AN, Chunikin SP. The experimental transmission of the tick-borne encephalitis virus by ixodid ticks (the mechanisms, time periods, species and sex differences). Parazitologiia. 1990;24:177–85.PubMedGoogle Scholar
Hartemink NA, Randolph SE, Davis SA, Heesterbeek JAP. The basic reproduction number for complex disease systems: defining R(0) for tick-borne infections. Am Nat. 2008;171(18462128):743–54.PubMedCrossRefGoogle Scholar
Randolph SE, Miklisová D, Lysy J, Rogers DJ, Labuda M. Incidence from coincidence: patterns of tick infestations on rodents facilitate transmission of tick-borne encephalitis virus. Parasitology. 1999;118:177–86.PubMedCrossRefGoogle Scholar
Randolph SE, Gern L, Nuttall PA. Co-feeding ticks: epidemiological significance for tick-borne pathogen transmission. Parasitol Today (Regul Ed). 1996;12:472–9.CrossRefGoogle Scholar
Labuda M, Kozuch O, Zuffová E, Elecková E, Hails RS, Nuttall PA. Tick-borne encephalitis virus transmission between ticks cofeeding on specific immune natural rodent hosts. Virology. 1997;235:138–43.PubMedCrossRefGoogle Scholar
Labuda M, Austyn JM, Zuffova E, et al. Importance of localized skin infection in tick-borne encephalitis virus transmission. Virology. 1996;219:357–66.PubMedCrossRefGoogle Scholar
Alekseev AN. Ecology of tick-borne encephaltis virus: part of Ixodidae ticks males in its circulation. Ecol Parasitol. 1992;1:48–58.Google Scholar
Jones LD, Kaufman WR, Nuttall PA. Modification of the skin feeding site by tick saliva mediates virus transmission. Experientia. 1992;48:779–82.PubMedCrossRefGoogle Scholar
Jones LD, Hodgson E, Williams T, Higgs S, Nuttall PA. Saliva activated transmission (SAT) of Thogoto virus: relationship with vector potential of different haematophagous arthropods. Med Vet Entomol. 1992;6:261–5.PubMedCrossRefGoogle Scholar
Jones LD, Matthewson M, Nuttall PA. Saliva-activated transmission (SAT) of Thogoto virus: dynamics of SAT factor activity in the salivary glands of Rhipicephalus appendiculatus, Amblyomma variegatum, and Boophilus microplus ticks. Exp Appl Acarol. 1992;13:241–8.PubMedCrossRefGoogle Scholar
Jones LD, Nuttall PA. Non-viraemic transmission of Thogoto virus: influence of time and distance. Trans R Soc Trop Med Hyg. 1989;83:712–4.PubMedCrossRefGoogle Scholar
Kaufman WR, Nuttall PA. Amblyomma variegatum (Acari: Ixodidae): mechanism and control of arbovirus secretion in tick saliva. Exp Parasitol. 1996;82:316–23.PubMedCrossRefGoogle Scholar
Rehacek J. Development of animal viruses and rickettsiae in ticks and mites. Annu Rev Entomol. 1965;10:1–24.CrossRefGoogle Scholar
Ebel GD, Kramer LD. Duration of tick attachment required for transmission of Powassan virus by deer ticks. Am J Trop Med Hyg. 2004;71:268–71.PubMedCrossRefGoogle Scholar
Slovák M, Kazimírová M, Siebenstichová M, et al. Survival dynamics of tick-borne encephalitis virus in Ixodes ricinus ticks. Ticks Tick Borne Dis. 2014;5:962–9.PubMedCrossRefGoogle Scholar
Kaufman WR, Nuttall PA. Rhipicephalus appendiculatus</i> (Acari: Ixodidae): dynamics of thogoto virus infection in female ticks during feeding on Guinea pigs. Exp Parasitol. 2003;104:20–5.PubMedCrossRefGoogle Scholar
Alekseev AN, Burenkova LA, Vasilieva IS, Dubinina HV, Chunikhin SP. Preliminary studies on virus and spirochete accumulation in the cement plug of ixodid ticks. Exp Appl Acarol. 1996;20:713–23. https://doi.org/10.1007/BF00051556.CrossRefPubMedGoogle Scholar
Jones LD, Hodgson E, Nuttall PA. Characterization of tick salivary gland factor(s) that enhance Thogoto virus transmission. Arch Virol. 1990;(Suppl 1):227–234.Google Scholar
Lieskovská J, Páleníková J, Širmarová J, et al. Tick salivary cystatin sialostatin L2 suppresses IFN responses in mouse dendritic cells. Parasite Immunol. 2015;37:70–8.PubMedCrossRefGoogle Scholar
Lieskovská J, Páleníková J, Langhansová H, Chmelař J, Kopecký J. Saliva of Ixodes ricinus enhances TBE virus replication in dendritic cells by modulation of pro-survival PI3K/Akt pathway. Virology. 2018;514:98–105.PubMedCrossRefGoogle Scholar
Conway MJ, Watson AM, Colpitts TM, et al. Mosquito saliva serine protease enhances dissemination of dengue virus into the mammalian host. J Virol. 2014;88:164–75. https://doi.org/10.1128/JVI.02235-13.CrossRefPubMedPubMedCentralGoogle Scholar
Jin L, Guo X, Shen C, et al. Salivary factor LTRIN from Aedes aegypti facilitates the transmission of Zika virus by interfering with the lymphotoxin-β receptor. Nat Immunol. 2018;19:342–53.PubMedCrossRefGoogle Scholar
Surasombatpattana P, Ekchariyawat P, Hamel R, et al. Aedes aegypti saliva contains a prominent 34-kDa protein that strongly enhances dengue virus replication in human keratinocytes. J Invest Dermatol. 2014;134:281–4.PubMedCrossRefGoogle Scholar
Rudenko N, Golovchenko M, Grubhoffer L, Oliver JH. Updates on Borrelia burgdorferi sensu lato complex with respect to public health. Ticks Tick Borne Dis. 2011;2:123–8. https://doi.org/10.1016/j.ttbdis.2011.04.002.CrossRefPubMedPubMedCentralGoogle Scholar
Anguita J, Ramamoorthi N, Hovius JW, et al. Salp15, an Ixodes scapularis salivary protein, inhibits CD4+ T cell activation. Immunity. 2002;16:849–59.PubMedCrossRefGoogle Scholar
Garg R, Juncadella IJ, Ramamoorthi N, et al. Cutting edge: CD4 is the receptor for the tick saliva immunosuppressor, Salp15. J Immunol. 2006;177:6579–83.PubMedPubMedCentralCrossRefGoogle Scholar
Hovius JW, de Jong MAWP, den Dunnen J, et al. Salp15 binding to DC-SIGN inhibits cytokine expression by impairing both nucleosome remodeling and mRNA stabilization. Plos Pathog. 2008;4:e31.PubMedPubMedCentralCrossRefGoogle Scholar
Tomás-Cortázar J, Martín-Ruiz I, Barriales D, et al. The immunosuppressive effect of the tick protein, Salp15, is long-lasting and persists in a murine model of hematopoietic transplant. Sci Rep. 2017;7:1–11.CrossRefGoogle Scholar
Schwan TG, Piesman J, Golde WT, Dolan MC, Rosa PA. Induction of an outer surface protein on Borrelia burgdorferi during tick feeding. Proc Natl Acad Sci USA. 1995;92:2909–13.PubMedPubMedCentralCrossRefGoogle Scholar
Dai J, Wang P, Adusumilli S, et al. Antibodies against a tick protein, Salp15, protect mice from the Lyme disease agent. Cell Host Microbe. 2009;6:482–92. https://doi.org/10.1016/j.chom.2009.10.006.CrossRefPubMedPubMedCentralGoogle Scholar
Hovius JWR, Ramamoorthi N, van’t Veer C, et al. Identification of Salp15 homologues in Ixodes ricinus ticks. Vector Borne Zoonotic Dis. 2007; 7(3)296–303. https://doi.org/10.1089/vbz.2006.0624.CrossRefPubMedGoogle Scholar
Kjaer TR, Le LTM, Pedersen JS, et al. Structural insights into the initiating complex of the lectin pathway of complement activation. Structure. 2015;23(2):342–51.PubMedCrossRefGoogle Scholar
Schuijt TJ, Narasimhan S, Daffre S, et al. Identification and characterization of Ixodes scapularis antigens that elicit tick immunity using yeast surface display. PLoS ONE. 2011;6(1):e15926.PubMedPubMedCentralCrossRefGoogle Scholar
Couvreur B, Beaufays J, Charon C, et al. Variability and action mechanism of a family of anticomplement proteins in ixodes ricinus. PLoS ONE. 2008;3(1):e1400.PubMedPubMedCentralCrossRefGoogle Scholar
Karasuyama H, Tabakawa Y, Ohta T, Wada T, Yoshikawa S. Crucial role for basophils in acquired protective immunity to tick infestation. Front Physiol. 2018;9:1769.PubMedPubMedCentralCrossRefGoogle Scholar
Mans BJ. Tick histamine-binding proteins and related lipocalins: potential as therapeutic agents. Curr Opin Investig Drugs. 2005;6(11):1131–5.PubMedGoogle Scholar
Mulenga A, Macaluso KR, Simser JA, Azad AF. The American dog tick, Dermacentor variabilis, encodes a functional histamine release factor homolog. Insect Biochem Mol Biol. 2003;33(9):911–9.PubMedCrossRefGoogle Scholar
Lieskovská J, Páleníková J, Langhansová H, et al. Tick sialostatins L and L2 differentially influence dendritic cell responses to Borrelia spirochetes. Parasit Vectors. 2015;8(1):275.PubMedPubMedCentralCrossRefGoogle Scholar
Rangel CK, Parizi LF, Sabadin GA, et al. Molecular and structural characterization of novel cystatins from the taiga tick Ixodes persulcatus. Ticks Tick Borne Dis. 2017;8(3):432–41. https://doi.org/10.1016/j.ttbdis.2017.01.007.CrossRefPubMedGoogle Scholar
Anderson JM, Valenzuela JG. Spit-acular entry: borrelia gets help from a tick salivary protein to move from the mammalian host to the arthropod vector. Cell Host Microbe. 2007;2(1):3–4.PubMedCrossRefGoogle Scholar
Bensaci M, Bhattacharya D, Clark R, Hu LT. Oral vaccination with vaccinia virus expressing the tick antigen subolesin inhibits tick feeding and transmission of Borrelia burgdorferi. Vaccine. 2012;30(42):6040–6.PubMedPubMedCentralCrossRefGoogle Scholar
Artigas-Jerónimo S, Villar M, Cabezas-Cruz A, et al. Functional evolution of Subolesin/Akirin. Front Physiol. 2018;9:1–17. https://doi.org/10.3389/fphys.2018.01612/full.CrossRefGoogle Scholar
Cotté V, Sabatier L, Schnell G, et al. Differential expression of Ixodes ricinus salivary gland proteins in the presence of the Borrelia burgdorferi sensu lato complex. J Proteomics. 2014;96:29–43.PubMedCrossRefGoogle Scholar
Boeuf A, Schnell G, Bernard Q, et al. Dissociating effect of salivary gland extract from Ixodes ricinus on human fibroblasts: potential impact on Borrelia transmission. Ticks Tick Borne Dis. 2018;10(2):433–41. https://doi.org/10.1016/j.ttbdis.2018.12.005.CrossRefPubMedGoogle Scholar
Gilbert L. Louping ill virus in the UK: a review of the hosts, transmission and ecological consequences of control. Exp Appl Acarol. 2016;68(3):363–74.PubMedCrossRefGoogle Scholar
Watson A, Wilson JD. Seven decades of mountain hare counts show severe declines where high-yield recreational game bird hunting is practised. J Appl Ecol. 2018;55(6):2663–72. https://doi.org/10.1111/1365-2664.13235.CrossRefGoogle Scholar
Stuen S, Granquist EG, Silaghi C. Anaplasma phagocytophilum—a widespread multi-host pathogen with highly adaptive strategies. Front Cell Infect Microbiol. 2013;3:31.PubMedPubMedCentralCrossRefGoogle Scholar
Cotté V, Bonnet S, Le Rhun D, et al. Transmission of Bartonella henselae by Ixodes ricinus. Emerg Infect Dis. 2008;14(7):1074–80.PubMedPubMedCentralCrossRefGoogle Scholar
Tomaso H, Otto P, Peters M, et al. Francisella tularensis and other bacteria in hares and ticks in North Rhine-Westphalia (Germany). Ticks Tick Borne Dis. 2018;9(2):325–9.PubMedCrossRefGoogle Scholar
Reed S, Coler RN, Mondal D, Kamhawi S, Valenzuela JG. Leishmania vaccine development: exploiting the host–vector–parasite interface. Expert Rev Vaccines. 2016;15(1):81–90. https://doi.org/10.1586/14760584.2016.1105135.CrossRefPubMedGoogle Scholar
Cecílio P, Pérez-Cabezas B, Fernández L, et al. Pre-clinical antigenicity studies of an innovative multivalent vaccine for human visceral leishmaniasis. Plos Negl Trop Dis. 2017;11(11):1–26.CrossRefGoogle Scholar
Study in Healthy Volunteers to Evaluate the Safety and Immunogenicity of AGS-v, a Universal Mosquito-Borne Disease Vaccine—Full Text View—ClinicalTrials.gov. Available at: https://clinicaltrials.gov/ct2/show/NCT03055000
Labuda M, Trimnell AR, Ličková M, et al. An antivector vaccine protects against a lethal vector-borne pathogen. PLoS Pathog. 2006;2(4):e27.PubMedPubMedCentralCrossRefGoogle Scholar
Wikel S. Ticks and tick-borne pathogens at the cutaneous interface: host defenses, tick countermeasures, and a suitable environment for pathogen establishment. Front Microbiol. 2013;4:337.PubMedPubMedCentralCrossRefGoogle Scholar
Contreras M, de la Fuente J. Control of infestations by Ixodes ricinus tick larvae in rabbits vaccinated with aquaporin recombinant antigens. Vaccine. 2017;35(9):1323–8.PubMedCrossRefGoogle Scholar
Hagman KE, Yang X, Wikel SK, et al. Decorin-binding protein A (DbpA) of Borrelia burgdorferi is not protective when immunized mice are challenged via tick infestation and correlates with the lack of DbpA expression by B. burgdorferi in ticks. Infect Immun. 2000;68(8):4759–64.PubMedPubMedCentralCrossRefGoogle Scholar
Pretorius A, van Kleef M, Collins NE, et al. A heterologous prime/boost immunisation strategy protects against virulent Ehrlichia ruminantium Welgevonden needle challenge but not against tick challenge. Vaccine. 2008;26(34):4363–71.PubMedCrossRefGoogle Scholar
Kovár L. Tick saliva in anti-tick immunity and pathogen transmission. Folia Microbiol. 2004;49(3):327–36.CrossRefGoogle Scholar
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Authors and Affiliations
Patricia A. Nuttall12Email author1.Department of ZoologyUniversity of OxfordOxfordUK2.Centre for Ecology & HydrologyWallingfordUK