Antimicrobial action of autologous platelet-rich plasma on MRSA-infected skin wounds in dogs
Antimicrobial action of autologous platelet-rich plasma on MRSA- infected skin wounds in dogs
Haithem A. farghali 0
naglaa A. AbdelKader 0
Huda o . AbuBakr 1
Samira H. Aljuaydi 1
Marwa S. Khattab 2
Rehab elhelw 3
Mahmoud elhariri 3
0 Department of Surgery, Anesthesiology and Radiology, Faculty of Veterinary Medicine, Cairo University , Giza, 12211 , Egypt
1 Department of Biochemistry and Chemistry of Nutrition, Faculty of Veterinary Medicine, Cairo University , Giza, 12211 , Egypt
2 Department of Pathology, Faculty of Veterinary Medicine, Cairo University , Giza, 12211 , Egypt
3 Department of Microbiology, Faculty of Veterinary Medicine, Cairo University , Giza, 12211 , Egypt
Effective antimicrobial preparations, other than antibiotics, are important for the treatment of potentially fatal drug-resistant infections. Methicillin-resistant Staphylococcus aureus (MRSA) is one of the leading causes of hospital-acquired and post- operative infections. Fortunately, the antimicrobial properties of platelet-rich plasma (PRP) against various microorganisms enable its potential use as an alternative to conventional antibiotics. The present work was designed to evaluate the hypothesized antimicrobial activity of PRP against MRSA infected skin wounds. Six adult male dogs were divided equally into control and pRp groups. Unilateral circular full-thickness skin wounds were created then a MRSA suspension was injected locally. Treatment started at 1st week post infection with subcutaneous infiltration of autologous activated PRP every week in the PRP group and with topical application of clindamycin cream twice daily in the control group. PRP decreased wound size and significantly increased wound contractility and re-epithelization, as confirmed by histopathological and immunohistochemical findings. Also PRP treated group showed significant decrease in ROS and redox imbalance with over expression of the TNF-α and VeGf A genes that indicate angiogenesis and maximum antibacterial activity after three weeks. In conclusion, CaCl2-activated PRP exhibited antimicrobial activity against MRSA infection, which improved the infected wound healing reepithelization and granulation tissue formation.
Published; xx xx xxxx
Wound infections with multidrug-resistant strains of pathogenic microorganisms are significant global health
problems. The economic loads and morbidity and mortality rates are immense and have attracted researchers to
find novel methods for infection management1,2
Skin has regenerative and protective capacities in addition to acting as a barrier between the body and the
outer environment3. A disturbance in the normal anatomic structure and functional integrity of the skin can be
described as a wound. Wound healing is a complex process that is mediated by molecular interactions associated
with recruitment of mesenchymal cells, proliferation, and regeneration of the extracellular matrix. The healing
process includes a pattern of events such as coagulation, inflammation, epithelialization, granulation tissue
formation and tissue remodelling4. Immediately after skin injury, platelet aggregation is induced to form a fibrin clot
that supports haemostasis and recruits several cell types to the wound5. These cellular activities are mediated by
cytokines and growth factors6. The inflammatory stage is induced by clotting and platelet degranulation and is
characterized by the release of serotonin, histamine and bioactive factors, which attract inflammatory cells such
as neutrophils, leukocytes, and macrophages to the wounded area4. During the healing process, reactive oxygen
species (ROS) are produced, resulting in peroxidation of the lipid components of the cell membrane and leading
to alterations in several antioxidant enzyme systems7. Excessive oxidative stress and imbalanced antioxidative
systems play important roles in the cellular senescence process8. Indeed, oxidative stress has been demonstrated
to be one of the most important causes of cellular senescence.
Platelet-rich plasma (PRP) treatment is an endogenously derived therapeutic technology that has great
importance in regenerative medicine due to its potential to accelerate and stimulate tissue healing9. PRP is an
autologous biological product obtained from the blood through a centrifugation process; this process yields a plasma
fraction with a platelet concentration higher than that in circulating blood10. The mechanism of action of PRP
depends on its content of functional platelets and growth factors, which can trigger cell activation and activate
related signalling pathways. Platelets secrete at least seven growth factors that are essential in the initiation of the
healing process, including the platelet-derived growth factor (PDGF) isomers PDGFaa, PDGFab and PDGFbb;
transforming growth factor (TGF)-b1, TGF-b2, vascular endothelial growth factor (VEGF), and epithelial growth
factor (EGF)11. These growth factors promote rapid increases in the numbers of undifferentiated mesenchymal
cells at the wound site during repair and healing. Therefore, PRP accelerates the regenerative process through the
high quantity of growth factors released by the platelets.
PRP is also believed to exert antimicrobial action. Recently, studies have evaluated the clinical and in vitro
antibacterial activity of platelet lysate (PL) against various bacteria12. Until now, the components controlling
the antimicrobial activity of PL have not been fully understood. PL is a complex mixture of plasma components
whose influences have not yet been studied in detail.
Methicillin-resistant Staphylococcus aureus (MRSA) is resistant to methicillin and other β-lactam
antibiotics. This resistance is due not only to β-lactamase production but also to penicillin-binding protein (PBP2a)
expression. MRSA is resistant to methicillin, amoxicillin, oxacillin, penicillin and many other common
antibiotics. In some cases, vancomycin is the only option for treatment. MRSA clinical infections involving the skin
and soft tissues can cause severe problems. Burns and surgical wounds are commonly infected by MRSA; in
these cases, the production of toxins can give rise to toxic shock syndrome, leading to fever and, in some cases,
death. Infections caused by MRSA include pneumonia, mastitis, skin infections (impetigo, staphylococcal scalded
skin syndrome and cellulitis), osteomyelitis, endocarditis and bacteraemia13. Such infections are now known as
community-associated MRSA infections. Additionally, MRSA is a major cause of hospital-acquired infection of
surgical wounds14. MRSA impair wound healing through secretion of virulence factors, such as the extracellular
adherence protein Eap, which interfere with the proliferation and migration capacities of keratinocytes by altering
their morphology and adhesive properties15. Furthermore, the planktonic and biofilm MRSA produce soluble
products that have an adverse effect on the migration and viability of human fibroblasts which is crucial in wound
healing process by inducing apoptosis16.
Recently, PL released from PRP has been investigated for its possible antibacterial effects. The aim of this study
was to evaluate the antimicrobial effect of PRP against MRSA wound infections and to examine the acceleration
of wound contraction and epithelization after subcutaneous (S/C) autologous PRP infiltration.
Methods and Materials
Animals. Six adult male mongrel dogs aged 3–5 years and weighing 25–30 kgs were used in the present study.
The animals were kept in separate kennels under standard environmental conditions (23± 1 °C, 55 ± 5%
humidity and a 12 h light/dark cycle). The dogs were given free access to water and were given maintenance rations twice
daily. The present study was approved by the Institutional Animal Care and Use Committee of Cairo University
(IACUC) and was performed after receiving ethical approval (approval number: CU/II/F/75/18). The experiment
was performed in accordance with relevant guidelines and regulations. All surgeries were carried out under
general anaesthesia, and all efforts were made to decrease animal suffering and the number of animals used.
Bacterial strain. The MRSA strain used was isolated from a wound of a clinically affected dog. The
specimen was inoculated onto a blood agar base (Oxoid, Basingstoke, Hampshire, England) to which 5% sheep blood
was added, and then the suspected MRSA colonies were subcultured on mannitol salt agar (Oxoid, Basingstoke,
Hampshire, England) at 37 °C for 18 to 24 h aerobically. Bacterial colonies showing typical characteristics of S.
aureus colonies (beta haemolytic colonies on blood agar and colonies with golden yellow pigmentation on
mannitol salt agar) were subjected to subculture on basic medium. Gram staining was applied as well as biochemical
testing for catalase and coagulase17. The isolate was then subjected to PCR for detection of mecC gene.
Antimicrobial susceptibility testing.
Kirby-Bauer disc diffusion method
An antimicrobial susceptibility test was carried out according to the Clinical Laboratory Standards
Institute18 guidelines on Muller Hinton agar (Oxoid, Basingstoke, England). The growth suspension was
prepared in 0.5 ml of the same broth medium, and the turbidity was adjusted to match that of 0.5
McFarland standards to obtain approximately 1 × 106 colony-forming units (CFU) per ml. The bacteria were
evenly spread with a sterile swab on Mueller-Hinton agar plates. Antibiotic discs were placed on the plates,
which were then incubated for 24 h at 37 °C. The S. aureus isolate were tested for resistance to nine different
antibiotics: chloramphenicol (CHL) (30 µg/disk), clindamycin (CLI) (2 µg/disk), erythromycin (ERY)
(15 µg/disk), novobiocin (NV) (30 µg/disk), ofloxacin (OFX) (5 µg/disk), cefoxitin (FOX) (30 µg/disk),
oxacillin (OXA) (1 µg/disk), trimethoprim-sulphamethoxezole (SXT) (23.75 µg/disk) and vancomycin (VAN)
(30 µg/disk). The disks were purchased from (Oxoid Ltd, Hampshire, UK).
Minimum Inhibitory Concentration
The MIC values of oxacillin and cefoxitin were determined by a broth microdilution method using
cation-adjusted Mueller-Hinton broth (Difco) and oxacilin and cefoxitin standard antibiotics (Sigma
Aldrich). The procedure and interpretation of the results were carried out according to the CLSI guidelines18
The laboratory breakpoints were as follow: oxacillin - resistant S. aureus: oxacillin MIC ≥4 μg/ml.
oxacillinsensitive S. aureus: oxacillin MIC ≤2 μg/ml. Cefoxitin -resistant S. aureus: cefoxitin MIC ≥8 μg/ml.
Cefoxitin - sensitive S. aureus: cefoxitin MIC ≤4 μg/ml.
Autologous pRp preparation. Whole-blood samples taken from the jugular vein of each dog were
prepared using the double-spin method, and activated by CaCl2 shortly before use as previously described19.
creation of full-thickness skin wounds, induction of MRSA wound infection and subdermal
application of pRp. Under general anaesthesia administered by injection, each dog was pre-medicated with
atropine sulphate (Atropine sulphate 1%, Adwia Co., Egypt) at a dose of 0.01 mg/kg BW given subcutaneously
and with xylazine HCl (Xyla-Ject 2%, Adwia Co., Egypt) at a dose of 1 mg/kg BW given intramuscularly. General
anaesthesia was induced using ketamine HCl (Sigma Tech., Egypt) at a dose of 10 mg/kg BW and was maintained
with ketamine HCl20. Under strict aseptic conditions, a unilateral circular full-thickness skin wound (3 cm in
diameter) was created on the thorax of each dog. Next, MRSA infection was induced with a density of 1.3 × 107
CFU/ml; 1 ml was applied for each cm2 of wound (Fig. 1). Treatment began one week after infection (considered
day 0 of the experiment) in both groups and continued for three successive weeks.
In the PRP treated group, the wounds were treated via S/C infiltration of 3 ml of autologous activated PRP
every week for three successive weeks. In the control group, the wounds were treated with clindamycin cream
(Pfizer, Egypt) twice daily21.
Wound fluid preparation. Wound fluid collection was performed at the clinical site with a standard
protocol previously described by Rayment et al.22. The wound fluid samples were used for assessment of lipid
peroxidation and glutathione reductase (GR) activity.
In vitro determination of PRP antibacterial activity by determination of the minimum
inhibitory concentration (Mic). The lowest concentration of an antimicrobial substance that inhibits the growth
of a microorganism is known as the MIC. The broth microdilution method was used for all PRP samples with
2-fold serial dilutions.
A suspension of MRSA was prepared in Mueller-Hinton broth (Oxoid, Basingstoke, England) and adjusted to
an optical density equal to 0.5 McFarland (1 × 108 CFU/ml).
After obtaining a concentration of 1 × 104 CFU/ml using appropriate dilutions, 20 µ l of each suspension was
inoculated into a 96-well microplate containing 180 µ l of a twofold serial dilution system (ranging from 1:2 to
1:2048) of PRP. Positive control wells were inoculated with MRSA in media alone, while negative control wells
contained Mueller-Hinton medium only. One positive control was established by incubating the MRSA
suspension in growth medium alone. The other positive control was used to confirm that CaCl2 did not have
antimicrobial activity, so this control was established by inoculating MRSA suspension into growth medium with CaCl2 at
a final concentration of 4.5 mM. The test was carried out in duplicate for both fresh PRP and CaCl2-activated PRP.
The results regarding the lowest dilution resulting in no visible bacterial growth were read after 24h of incubation
at 37 °C23. The MIC values are expressed as dilutions from the initial concentration.
Clinical evaluation. For clinical evaluation, digital photographs were taken of both control and PRP treated
wounds in the presence of a metal ruler near the wounds to measure wound contraction and epithelization at days
7, 14, and 21 after infection in comparison to day 0. Wound contraction of both control and PRP treated wounds
was calculated according to the following equations24:
1. Percent of the wound size at day (x) = Ax mm2/A0 mm2 × 100
2. Percent of the wound contraction (%) = 100 − percent wound size at day (x).
Wound epithelization was also calculated according to the following equation:
3. Percent epithelization (%) = Ae mm2/Ax mm2 × 100.
where A0 is the original wound area, Ax is the wound area at the time of imaging and Ae is size of epithelization
area on day (x)
Day (x) = at days 7, 14, and 21
Quantitation of bacterial load before and after treatment. The surface of each wound was flushed
with normal saline. Under strict aseptic conditions, biopsies were taken from the wounds in both groups before
treatment and then weekly until the third week of treatment. Then, the biopsies were weighed and directly placed
into 1 ml of normal saline for homogenization. A 0.5 ml aliquot of each homogenate was taken and placed in a
dilution test tube containing 4.5 ml of normal saline. After thorough mixing, 0.5 ml of the mixture was transferred
to form a ten-fold dilution series of 101- to 108-fold dilutions. A volume of 0.01 ml of each dilution was evenly
spread on oxacillin resistance screening agar base (ORSAB) (Oxoid Limited, England), a selective medium for
MRSA. The plates were incubated at 37°C for 24 h. The number of colonies per gram of tissue was calculated with
the following formula25:
4. CFU/g of tissue = C × D × 1/W × 0.01
where C is the total CFU, D is the dilution factor, 1 is the volume of normal saline in ml, W is the weight of the
tissue, and 0.01 is the volume of inoculums
Evaluation of oxidative and antioxidative stress biomarkers. The malondialdehyde (MDA)
concentration, an index of lipid peroxidation, was assessed as described by Ohkawa et al.26. GR is essential for the
glutathione redox cycle to maintain an adequate level of reduced glutathione, which serves as an antioxidant. GR
catalyses the reduction of oxidized glutathione in the presence of NADPH + H. The decrease in absorbance at
340 nm was measured27.
Quantitative real-time PCR (qPCR) evaluation of the tumour necrosis factor-alpha (TNF-α) and
vascular endothelial growth factor-alpha (VEGFA) genes. As described previously by AbuBakr et al.28,
total RNA isolated from skin biopsies of both groups related to day zero was subjected to real-time PCR (qPCR)
using 0.5 mM of each primer (TNF-α, VEGFA, and GAPDH as an internal control) (Table S1). The analysis
was performed using a Bio-Rad iCycler thermal cycler and a MyiQ real-time PCR detection system. Each assay
included triplicate samples for each tested cDNA and no-template negative control. The expression in each
sample relative to that in the control samples, which were skin biopsies taken at time zero, were calculated using the
equation 2−ΔΔCT 29.
Histopathological evaluation. Skin biopsy samples were collected from wound edges (3–4 mm) of both
control and PRP treated wounds 1 week before treatment and in the 1st, 2nd, and 3rd week after treatment. The
samples were fixed in 10% neutral formalin buffer. The fixed samples were then processed using the paraffin
embedding technique. Tissue sections 3–5 µ m thick were made using a microtome (Leica 2135, Germany) and
were subjected to routine haematoxylin and eosin staining and Masson’s trichrome (MTC) staining for
morphometric analysis of fibrous tissue. The wound sites were examined using a light microscope (Olympus XC, Tokyo,
Japan) and were photographed using a camera (Olympus, Tokyo, Japan). Lesion scoring for re-epithelization,
polymorphonuclear leukocyte (PMNL) infiltration, fibroblast numbers and newly formed blood vessel numbers
was performed in a blinded manner according to a semiquantitative scoring system with the following scores:
0 (absent), 1 (minimal), 2 (mild), 3 (moderate) and 4 (marked)19,30. In more detail, a score of zero indicated the
absence of epithelization, fibroblasts, newly formed blood vessels, and PMNL infiltration; a score of 1 indicated
increased thickness of the cut edges of the epithelium, the presence of few fibroblasts and newly formed blood
vessels, and minimal PMNL infiltration; a score of 2 indicated migration of epithelial cells, the presence of
moderate numbers of fibroblasts and newly formed blood vessels, and moderate PMNL infiltration; and a score of 3
indicated epithelial bridging of the incision, the presence of many fibroblasts and newly formed blood vessels, and
considerable PMNL infiltration. The epithelial thickness (micron) was measured in three fields/wound at 200X
using TS View software. Furthermore, a summary score for each animal based on wound examination was
performed to evaluate the degree of cellular and bacterial debris according to the following scale (absent (
Control (before infection)
First week after infection
Second week after infection
Third week after infection
PRP with PL
present < 33% (
), present 33–66% (
), intensively present (
) and the degree of stromal necrosis according to the
following scale; 1 (minimal) < 10%, 2 (mild) 10–25%, 3 (moderate) up to 50%, 4 (marked) up to 75%31,32.
The area percent of fibrous tissue stained with MTC at the wound sites was measured in three captured
photographs (200x) per slide from both groups using ImageJ analysis software.
immunohistochemical (iHc) staining. Alpha smooth muscle actin (α-SMA) was immunohistochemically
stained in the skin sections. The paraffin-embedded tissue was sectioned and mounted on positively charged slides
before deparaffinization using xylene and rehydration using descending grades of ethyl alcohol. According to the
manufacturer’s protocol, antigen retrieval was performed, and the slides were immunohistochemically stained using
α-SMA primary antibodies (Novus Biologicals, Europe) prepared in rabbits and an avidin-biotin peroxidase
complex kit (Dako North America, Inc.). Using Image J, the percent area of positive staining was determined.
Statistical analysis. Statistical analysis was performed with the statistical package SPSS, version 8.0 (SPSS
Inc., Chicago, IL, U.S.A.). Statistical analysis of the data was carried out using Student’s t test and Mann Whitney
test for lesion scores. The results are expressed as the mean± S.E.M. P values less than 0.05 were considered
Ethics approval and Consent for publication. This study was approved by the Animal Use and Care
Committee at Faculty of Veterinary Medicine, Cairo University, Egypt. This study was performed after receiving
ethical approval number: CU/II/F/75/18.
All authors have reviewed the manuscript and approved its submission for publication.
Bacteriological findings. Gram staining showed gram-positive cocci appearing in grape-like clusters, and
biochemical testing indicated positivity for catalase and coagulase. These colonies were considered S. aureus. The
isolated strain was mecC gene-positive.
The MRSA isolate used in this study was completely resistant to antibiotics such as penicillin, oxacillin,
erythromycin and ceftazidime and sensitive to the antibiotic vancomycin (Fig. S1). Resistance pattern was
The MIC results of oxacillin and cefoxitin were determined by a broth microdilution method revealed
oxacillin MIC >8 μg/ml and cefoxitin MIC >16 μg/ml, which confirming that the strain is MRSA.
While, the In vitro determination of PRP antibacterial activity by MIC showed values ranged from 1:4 to 1:64
(Fig. S2 and Table 1). CaCl2-activated PRP inhibited the growth of MRSA at a dilution of 1:4 in the sample taken
before conducting the experimental infection. The MIC against MRSA revealed a pattern of 4-fold increases; it
reached 1:16 after one week of treatment with PRP and continued increasing through the second week of
treatment to inhibit the growth of MRSA at 1:64 in the third week of treatment.
In contrast, the PRP in which platelets did not release biologically active components did not show any
Clinical findings. After one week of infection, the wound area (mm2) had reached 93.0 ± 4.4 in the control
group and 93.0 ± 1.7 in the PRP treated group. After 1 week of PRP treatment, the wound size was smaller in
the treated group than in the control group. The wound size at week 1 was 24.1± 1.6 mm2 in the control group
(Fig. 1) and 8.6 ± 0.7 mm2 in the PRP treated group (Fig. 2). At the 2nd week, the wound size in the control group
was 25.0 ± 10.6 mm2 (Fig. 1), while that in the PRP treated group was 2.2 ± 0.2mm2 (Fig. 1). At the 3rd week, the
wound size was 5.3 ± 2.9 mm2 (Fig. 2) in the control group and 0.5 ± 0.2 mm2 in the PRP treated group (Table 2).
In this context, a significant size reduction (P < 0.05) was found after 1 week of treatment (Fig. 2). The wound
contraction percentage was elevated (P < 0.05) in the PRP treated group compared to the control group at all
intervals, with a significant elevation at week 1 (Fig. 2). The re-epithelization rate percentage was significantly
increased in the PRP treated group at week 2 (Fig. 2).
Bacterial load assessment before and after treatment. The bacterial count from the wound biopsies
in the control group decreased from 2.86 × 107 at the beginning of the infection period to 1.03 × 102 in the third
week of the treatment period, while that in the PRP treated group decreased from 2.65 × 107 at the beginning of
the infection period to 2 × 101 in the third week of treatment (Table 3).
Biochemical findings of oxidative and antioxidative stress biomarkers in wound fluid. In the 1st
week, the concentration of MDA was significantly increased in the positive control group (14.3 ± 1.5) compared
to the PRP treated group. In addition, the MDA concentration was increased in the PRP treated group at the 2nd
week (46 ± 1.5); however, it was significantly lower in the PRP treated group than in the control group at the 3rd
week (Fig. 3a). The activity of GR was significantly higher in the positive control group than in the PRP treated
group throughout the entire experimental period (Fig. 3b).
Gene expression findings. TNF-α gene expression findings. By the 3rd week, the expression of the TNF-α
gene had significantly decreased by 1.8-fold in the PRP treated group, whereas it had decreased by 37-fold in the
positive control group but still significantly higher than PRP group relative to day zero; however, in the 1st week,
TNF-α expression was significantly higher in the PRP treated group than in the control group (Fig. 4).
VEGFA gene expression findings. The expression of the VEGF gene was significantly higher in the PRP
treated group (13.2, 28.2, 85) fold respectively than in the control group throughout the experimental period (Fig. 5).
Histopathological findings. After 1 week of induction and concurrent infection, the skin wounds
exhibited necrotic tissue and bacterial aggregation together with increased epithelial thickness at the cut edges, many
PMNLs, a moderate degree of fibroblast infiltration and the presence of angiogenesis (Fig. 6a,b). Stromal necrosis,
C + ve
C + ve
C + ve
I- Wound Size (mm2)
and bacterial debris recorded no significant difference between the control (2.5± 0.5; 3.5 ± 0.5) and PRP wound
(2.5 ± 0.5; 3 ± 0) before treatment initiation. After the 1st week of treatment, the epithelium had begun to migrate
beneath the scab in the control and PRP treated wounds. PMNLs were still observed in both wounds, but the
fibroblasts were more prominent in the PRP treated wounds than in the control wounds. A moderate degree of
angiogenesis was observed in the control and PRP treated wounds (Fig. 6c,d). At the first week, there were no
significant differences in the scored variables between the groups. The epithelial thickness wasn’t significantly
different between control (84.22 ± 9.29) and PRP treated wound (94.1 ± 16.89). The necrosis and bacterial debris
scored 2.5 ± 0.5 and 2.5 ± 0.5 in PRP wound which was comparable to control wound (2.5 ± 0.5, 3 ± 0) at 1st week.
After the 2nd week of treatment, there was remarkable improvement in the PRP treated wounds. The
epithelium had bridged the wound area with almost complete regeneration in the PRP treated wounds; in
contrast, the control wounds showed epithelial migration without bridging of the wound area. PMNLs were almost
absent in the PRP treated wounds compared to the control wounds, which still showed PMNL infiltration. The
re-epithelization and PMNL scores were significantly higher in PRP treated wounds than in control wounds. The
epithelial thickness significantly increased in PRP treated wound (193.69± 20.38) compared to control wound
(67 ± 9.26) at this time. In addition, fibrous connective tissue with collagen fibres was well formed in the PRP
treated wounds compared to the control wounds (Fig. 6e,f ). The necrosis and bacterial debris were not
significantly different between the control group (2 ± 1, 2.5 ± 0.5) and PRP group (2 ± 0, 1 ± 0).
At the 3rd week of treatment, the epithelium was completely regenerated in both the PRP treated wounds and
control wounds, but it was hyperplastic in the control wounds. The degree of re-epithelization in PRP treated
wounds was also significantly greater than that in control wounds. Mature fibrous connective tissue was observed
filling the PRP treated wounds beneath the epithelium but was less often observed in the control wounds (Fig. 6g,h).
At the 3rd week of treatment, the epithelium was completely regenerated in both the PRP treated wounds and control
wounds, but it was hyperplastic in the control wounds. The epithelial thickness significantly increased in the control
group (156.73 ± 13.7) compared to PRP treated wound (112.2 ± 9.66). However, the degree of re-epithelization in
PRP treated wounds was significantly greater than that in control wounds. Mature fibrous connective tissue was
observed filling the PRP treated wounds beneath the epithelium but was less often observed in the control wounds
(Fig. 6g,h). The degree of necrosis and bacterial debris recorded a significant difference (P< 0.034) between control
(2.5 ± 0.5, 2.5 ± 0.5) and PRP group (1 ± 0, 1 ± 0) as there were still area of necrosis in control group.
Using MTC stain, collagen was labelled at the wound site. Collagen staining was poor after the 1st week of
treatment (Fig. 7a,b). The presence of collagen increased with time, however, and it was more prominent in PRP
treated wounds than in control wounds at the 2nd week of treatment (Fig. 7c,d). The collagen fibres were well
organized and had a parallel orientation in PRP treated wounds (Fig. 7e,f). The area percent of collagen positive
staining was significantly higher in the PRP treated group than in the control group at all time points (Fig. 8).
Immunohistochemical findings. At the 1st week after infection, angioblasts and few fibroblasts stained
brown for α-SMA in the control wounds, whereas angioblasts and many fibroblasts stained brown for α-SMA in
the PRP treated wounds (Fig. 9a,b). The percentage of positively stained cells was significantly increased in PRP
treated wounds (6.649 ± 0.13) compared to control wounds (2.618 ± 0.12).
identification of 7 antimicrobial peptides from human platelets: fibrinopeptide A, fibrinopeptide B, thymosin
β-4, platelet basic protein, connective tissue-activating peptide 3, RANTES and platelet factor. In vitro assays have
revealed the antimicrobial activity of these peptides against E. coli, S. aureus, Candida albicans and Cryptococcus
neoformans. In addition, the release of platelet α-granules in high concentrations after platelet aggregation
contributes to PL antimicrobial action. Platelet α-granules contain not only growth factors and antimicrobial peptides
but also catecholamines, serotonin, osteonectin, von Willebrand factor, proaccelerin, and other substances40,41.
In this study, the concentrations of MDA and the activity of GR were significantly increased in the control
group compared to the PRP treated group. The increases in MDA concentrations and subsequent elevations in
GR activity occurred to compensate for the depletion of GSH storage in the positive control group treated with
traditional ointment, indicating ROS accumulation and redox imbalance.
Our results are consistent with those of Jia et al.11, who stated that the effects of PRP against UV-related
photoaging alter the oxidant enzymatic system by inducing ROS through peroxidation of lipid components of the
cell membrane. In addition, those authors proved that PRP preserves mouse dermal fibroblasts (MDFs) by
reducing the accumulation of free radicals through enhancement of the intracellular activity of antioxidant enzymes,
especially GPx. Tohidnezhad et al.42 reported an ameliorative effect of PRP mediated by activation of antioxidant
response elements in the Nrf3-ARE pathway in a tenocyte culture model. Furthermore, PRP has been
demonstrated to exert a protective effect in ischaemia by reducing myeloperoxidase, MDA, and nitric oxide
concentrations and increasing SOD activity43.
Numerous studies have addressed the ability of different exogenous compounds, such as PRP, to induce
endogenous protection mechanisms by decreasing oxidative stress damage via ubiquitous growth factors44. These
growth factors have antioxidative effects; for example, IGF decreases oxidative stress and repairs damaged DNA
and RNA45. Additionally, IGF, HGF, and PDGF contribute to resistance against oxidative stress by activating the
With regards to a direct antioxidant effect, PRP acts as an apoptosis regulatory messenger as it reduces the
expression of ASK-1, a typical member of the mitogen-activated protein kinase kinase kinase (MAPKKK) family
and the critical component in ROS-induced apoptosis43,48.
In the present study, the expression of the TNF-α gene significantly increased in the 1st week and gradually
decreased by the 3rd week in the PRP treated group, in contrast to the case in the control group. In addition,
VEGFA gene expression was significantly increased in the PRP treated group compared to the control group.
Based on the abovementioned results, PRP treatment is a tool that enables plentiful growth factor application
at wound sites to stimulate the production of extracellular matrix materials and collagen with smaller amounts of
plasma than conventional treatments. Previous studies4,49 have quantified growth factor, cytokine, and chemokine
secretion after calcium activation of platelets. These studies revealed high concentrations of TGF, EGF, PDGF,
VEGF, IGF and FGF, which were induced along with pro- and anti-inflammatory cytokines such as IL-4, IL-8,
IL-13, IL-17, TNF-α and INF-α. Additionally, Nurden et al.50 confirmed that PRP increases growth factor levels
by 30–40% and subsequently accelerates wound healing.
Pro-inflammatory cytokines play important roles in controlling the oxidative damage that occurs with chronic
conditions such as diabetic ulcers, enhancing short-term wound recovery and reducing inflammation and pain at
the site of injury51,52. VEGF is considered a major regulator of vascular permeability, angiogenesis,
lymphangiogenesis, proliferation, morphogenesis, migration, and survival of endothelial cells in physiological and
pathological processes53. During wound healing, VEGF may exert paracrine action on TGF-β to promote the generation
of new blood vessels at the site of the wound54. Additionally, the findings of Hui et al.53 support a synergistic effect
of TGF-β and VEGF-α in inducing vascular regeneration under conditions of sufficient oxygen and nutrients for
repair of multiple microthermal damage areas.
Recently, VEGF application has been confirmed to accelerate wound repair in both experimental and clinical
studies by elevating angiogenesis, epithelialization and granulation in both mouse and human patients55 and by
enhancing tissue perfusion of skin flaps in dogs56.
In the current study, PRP treated infected wounds exhibited accelerated healing rates compared to untreated
infected wounds. Wound size was decreased and wound contractility was increased significantly at the 1st week
in the PRP treated group. PRP treatment significantly accelerated re-epithelization by the 2nd week and promoted
fibrous tissue formation. These findings are similar to those of previous studies showing that all tested forms of
PRP significantly enhanced the mean histopathological scores of epithelialization, inflammation, and fibrosis57.
It has been suggested that PRP reduces excessive early- and late-phase inflammation during wound healing. PRP
may also act together with macrophages to enhance tissue healing and regeneration58.
Although the inflammatory response at the site of a wound is a crucial factor in wound healing because it
recruits leukocytes that release cytokines and inflammatory mediators, excessive and persistent inflammation
may result in the chronic presence of unhealed wounds59. The contamination of wounds with bacteria, especially
those resistant to antibiotics, delays the healing process by causing ongoing inflammation60. PRP treatment of
infected wounds has exhibited promising results, lowering the inflammation scores in MRSA-infected wounds59.
Likewise, in our study, PMNL was reduced in PRP treated wounds, suggesting the regression of inflammation.
Clinically, there were greater wound contraction percentages (P < 0.05) in PRP treated wounds than in control
wounds at all time points, with significant increases at week 1. These findings were confirmed by the
histopathological findings. α-SMA is expressed constantly in pericytes and/or smooth muscle cells of newly formed small
vessels in wounds. It is also expressed in fibroblasts from the 6th to the 15th day after wounding. Fibroblasts with
α-SMA expression are known as myofibroblasts. These cells develop gradually from granulation tissue fibroblasts
and for a short time express a marker of smooth muscle differentiation61. The actin cytoskeleton is subjected
to extensive remodelling during myofibroblast differentiation62. Myofibroblasts produce type I collagen during
tissue repair and are also responsible for collagen remodelling62. In the present study, PRP treatment increased
the expression of α-SMA in fibroblasts infiltrating skin wounds compared to the control treatment. Likewise,
previous studies have demonstrated that PRP addition increases the expression of α-SMA protein and results
in marked contraction in a collagen gel model. PRP also stimulates proliferation and dermal fibroblast
differentiation into myofibroblasts, which promotes wound contraction and healing63. In addition, corneas given
topical PRP treatment exhibit higher proportions of α-SMA-positive myofibroblasts than control corneas. Notably,
myofibroblast accumulation occurs due to modulation of the TGF-β pathway64. Therefore, in a matter of time
PRP have accelerated fibrosis and neovascularization which is crucial in wound contraction and healing. On the
other side, PRP could also have counteracted the negative impact of MRSA infection on the migration of
fibroblasts leading to enhanced fibroblast migration.
The enhanced healing of PRP treated wounds may be partially attributable to enhanced neovascularization
in the wound beds65. Neovascularization is an interaction between cells and angiogenic growth factors. VEGF
plays a key role in endothelial cell proliferation and migration, leading to capillary sprouting or angiogenesis.
In addition, basic FGF and PDGF chemoattract smooth muscle cells and cause smooth muscle cell growth and
vessel enlargement66. In the current study, α-SMA-stained blood vessels were clearer in PRP treated wounds than
in control wounds, suggesting enhanced angiogenesis.
We suggest that a new alternative should be presented given both the antimicrobial activity of PL and the
healing effect of PL on infected wounds, especially MRSA-infected wounds. Antibiotic resistance is an
increasing problem, and such a treatment is crucial in addressing MRSA infection-related delayed healing. In vitro and
in vivo data suggest that this approach may accelerate the healing process; however, further studies are required
to clarify the antimicrobial effects of PL.
In conclusion, PRP accelerated the healing of infected skin wounds because it exerted antibacterial activity and
rapidly reduced inflammation, which allowed rapid re-epithelization and granulation tissue formation. The
antibacterial activity of PRP was proven in vitro by MIC analysis and in vivo by quantitation of the bacterial loads in
wound biopsies. So, it can augment antibiotic therapy.
All data generated or analyzed during this study are included in this published article [and its supplementary
The authors declare that there are no sources of funding to be acknowledged.
N.A. has performed the clinical evaluation of induced skin wounds, H.O.A. and S.H.A. have prepared the PRP and
wound fluid and have performed the biochemical and molecular evaluation of induced skin wound, M.S.K. has
performed the pathological, R.E. and M.E. have prepared bacteria strain and have performed bacteriological
studies and H.F. has supervised and reviewed this study. All authors have contributed in writing this article.
Competing Interests: The authors declare no competing interests.
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Additional information Supplementary information accompanies this paper at https://doi.org/10.1038/s41598-019-48657-5.