Investigation of serum oxidative stress levels in patients with nasal polyps

Dicle Tıp Dergisi / Dicle Med J (2018) 45 (1) : 1 - 7 Original Article / Özgün Araştırma Investigation of serum oxidative stress levels in patients with nasal polyps Adnan Ekinci1 1 Hitit University, Faculty of Medicine, Otorhinolaryngology Department, Çorum, Turkey ORCID: 0000-0001-7725-8024 Geliş: 25.09.2017 Revizyon: 13.12.2017 Kabul Tarihi: 11.01.2018 Abstract Objective: To compare serum total antioxidant status (TAS), total oxidant status (TOS), and paraoxonase (PON1) levels between nasal polyp patients and heal thy subjects. Method: In the present study, The nasal polyp group includes 26 males an 22 females, a total of 48 patients (mean age 37.11±12.07 years), while the control group included 22 males and 24 females, a total of 46 healthy subjects (mean age 36.91±13.03) years. Groups were compared in terms of serum TAS, TOS and PON1 levels. Results: The mean TAS level was 1.248 mmol/L in the control group, it was 1.176 mmol/L in the patient group (p=0.044). The mean TOS level was 3.443 mmol/L in the control group, and it was 6.720 mmol/L in the patient group (p=006). Nasal polyp and control groups did not show any significant difference for PON1 (301.15 U/L vs. 295.75 U/L,p>0.05). Conclusion: Serum TOS levels were significantly higher, and TAS levels were significantly lower in patients with nasal polyposis compared it the control group. There was no significant difference between the patients with nasal polypand the control group in terms of the PON1 values. Keywords: Nasal Polyp, Total Oxidant Status, Total Antioxidant Status, Paraoxonase. DOI: 10.5798/dicletip.407237 Yazışma Adresi / Correspondence: Adnan Ekinci, Üçtutlar Mh, Ahçılar 16,Sokak, Ada Aparmant B Blok no:15 19000, Çorum, Turkey e-mail: 1 Ekinci A. Nazal Polip’li Hastalarda Serum Oksidatif Stres Düzeylerinin Araştırılması Özet Amaç: Nazal polip hastaları ile sağlıklı bireyler arasında serum total antioksidan status (TAS), total oksidan status (TOS) ve paraoksonaz (PON1) düzeylerini karşılaştırmak. Yöntemler: Çalışmamızda nazal polip grubunda 26 erkek, 22 kadın, toplam 48 hasta (ortalama yaş 37.11 ± 12.07 yıl), kontrol grubunda 22 erkek ve 24 kadın olmak üzere toplam 46 sağlıklı birey (ortalama yaş 36.91 ± 13.03) dahil edildi. Gruplar serum TAS, TOS ve PON1 düzeyleri açısından karşılaştırıldı. Bulgular: Ortalama TAS düzeyi kontrol grubunda 1.248 mmol / L, hasta grubunda 1.176 mmol / L idi (p = 0.044). Kontrol grubunda ortalama TOS düzeyi 3.443 mmol / L, hasta grubunda 6.720 mmol / L bulundu (p = 006). nazal polip ve kontrol grupları PON1 düzeyleri bakımından anlamlı bir fark görülmedi. (301.15 U / L' vs295.75 U / L, p> 0.05). Sonuç: Serum TOS düzeyleri, kontrol grubuna göre nazal polipozisli hastalarda anlamlı olarak daha yüksek ve TAS düzeyi anlamlı olarak düşük olarak tespit edildi. Nazal polip ve kontrol grubu arasında PON1 değerleri açısından anlamlı fark tespit edilemedi. Anahtar kelimeler: Nazal Polip, Total Oksidan Durumu, Total Antioksidan Durum, Paraoksonaz INTRODUCTION In recent years, a number of studies focused on the oxidant/antioxidant molecules due to their roles in diseases. The concentration of each oxidant molecule in the plasma can be determined alone, but the oxidant molecules can interact with each other. Because of this interaction of antioxidant molecules, a new method, total oxidant status (TOS) measurement, was developed by Erel and its usage became widespread recently.7,8. Similarly, measurement of the total antioxidant status (TAS) gives more valuable information than the measurement of the levels of antioxidants separately. Therefore, measurement of TAS, which gives the total antioxidant status, became more common than the measurement of individual antioxidants7. Paraoxonase (PON1), which works as a strong antioxidant, is a high density lipoprotein (HDL) associated hydroxylase that can hydroxylase paraxone which is a potent inhibitor of cholinesterases 9,10. In this study, we aimed to determine TAS, TOS and PON1 levels in serum in order to determine oxidative stress levels in nasal polyposis patients. We want to contribute to the Nasal polyp is a benign disease characterized by the growth of the nasal mucosa to the nasal cavity due to various causes and they are the most common causes of nasal masses1. Allergic, infectious, mechanical, immunological and biochemical factors have been considered to play a role in the etiopathogenesis2.Nasal polyp tissue contains high amounts of eosinophils, neutrophils, macrophages, lymphocytes and myofibroblasts, which lead to overproduction of reactive oxygen species (ROS). Epithelial cell damage occurs due to high amount of those inflammatory cells, particularly eosinophils, and the products of those cells, including ROS35. There is a balance between oxidant and antioxidant molecules in the body. In case this balance is changed in favor of oxidants, oxidative stress, and hence free radical damage occurs6. Free oxygen plays a role in the pathogenesis of many diseases such as radicals, diabetes, atherosclerosis, Bechet's disease, tumors, rheumatological and dermatological diseases 4. 2 Dicle Tıp Dergisi / Dicle Med J (2018) 45 (1) : 1 - 7 literature because of the limited number of studies on this issue. METHODS The present study was carried out between January 15 and July 01, 2016 in Hitit University, otolaryngology department. Gaziosmanpaşa University Clinical Research Ethics Committee approved to work. (Decree No. 15, KAEK190).A total of 48 patients diagnosed with nasal polyps, and 46 healthy subjects were included in the study. There were 26 were males and 22 were females with a mean age of 38.1 years in the nasal polyp group. The control group consisted of 46 subjects, 22 males and 24 females, with a mean age of 36.9 years. All participants provided their written informed consents for participating in the study. The diagnosis of the patients was made by direct and endoscopic examinations. The nasal polyp patients with atherosclerosis, malignant tumor, hypertension, chronic inflammatory diseases, diabetes mellitus and nasal disorders such as antrochoanal polyp, inverted papilloma were excluded. The subjects in the control group did not have any systemic diseases, bronchial asthma or nasal disorders including chronic sinusitis, nasal polyps and allergy. None of the patients or controls were smokers or consumed alcohol. Serum samples were collected from patients with nasal polyps and control group before any medical or surgical treatment was applied. Ten milliliters of venous blood sample was drawn into vacuumed test tubes between 8:00 and 10:00 AM to measure TAS, TOS and PON1. After waiting for 30-45 minutes, blood was centrifuged at 4000 RPM for 10 minutes, serum was separated, and stored in an Eppendorf tube at suitable cold. Biochemical Measurements: TAS and TOS measurements developed by Erel were used for measurements.7,9 Determination of TOS was performed by a full automatic kit developed by Erel (RelAssay Diagnosti (...truncated)