Proteinase-activated receptor-2: two potential inflammatory mediators of the gastrointestinal tract in Atlantic salmon

Journal of Inflammation, Oct 2008

Proteinase-activated receptor 2 (PAR-2), activated by trypsin and other serine proteinases, is a key initiator of inflammatory responses in the intestine of mammals. Atlantic salmon fed diets with standard qualities of soybean meal (SBM) show enteritis of the distal intestine as well as increased activity of trypsin in both luminal contents and wall tissue. Luminal trypsin activity may possibly be involved in immune related disorders of the intestine also in Atlantic salmon via activation of PAR 2. In the present study our aim was to investigate if PAR-2 play a role in SBM induced enteritis. We performed multiple alignments based on nucleic acid sequences of PAR-2 from various animals available from public databases, and designed primers for use in cloning of the Atlantic salmon PAR-2 transcript. We further cloned and characterized the full length sequence of Atlantic salmon PAR-2 and investigated the expression in both early and chronic stages of SBM induced enteropathy. Two full length versions of PAR-2 cDNA were identified and termed PAR-2a and PAR-2b. Expression of the two PAR-2 transcripts was detected in all 18 tissues examined, but most extensively in the intestine and gills. A significant up-regulation in the distal intestine was observed for the PAR-2a transcript after 1 day feeding diets containing SBM. After 3 weeks of feeding, PAR-2a was down-regulated compared to the fish fed control diets. These findings may indicate that PAR-2a participates in inflammatory responses in both the early and later stages of the SBM enteropathy. In the chronic stages of the enteropathy, down-regulation of PAR-2a may indicate a possible desensitization of the PAR-2a receptor. Expression of PAR-2b was not altered in the first 7 days of SBM feeding, but a significant up regulation was observed after 3 weeks, suggesting a putative role in chronic stages of SBM induced enteritis. The expression differences of the two PAR-2 transcripts in the feed trials may indicate that they have different roles in the SBM induced enteritis.

A PDF file should load here. If you do not see its contents the file may be temporarily unavailable at the journal website or you do not have a PDF plug-in installed and enabled in your browser.

Alternatively, you can download the file locally and open with any standalone PDF reader:

http://www.journal-inflammation.com/content/pdf/1476-9255-5-18.pdf

Proteinase-activated receptor-2: two potential inflammatory mediators of the gastrointestinal tract in Atlantic salmon

Journal of Inflammation Proteinase-activated receptor-2: two potential inflammatory mediators of the gastrointestinal tract in Atlantic salmon Jim Thorsen 0 Einar Lilleeng 0 Elin Christine Valen 0 shild Krogdahl 0 0 Address: Aquaculture Protein Centre, Basic Science and Aquatic Medicine, Norwegian School of Veterinary Science , Oslo , Norway Proteinase-activated receptor 2 (PAR-2), activated by trypsin and other serine proteinases, is a key initiator of inflammatory responses in the intestine of mammals. Atlantic salmon fed diets with standard qualities of soybean meal (SBM) show enteritis of the distal intestine as well as increased activity of trypsin in both luminal contents and wall tissue. Luminal trypsin activity may possibly be involved in immune related disorders of the intestine also in Atlantic salmon via activation of PAR 2. In the present study our aim was to investigate if PAR-2 play a role in SBM induced enteritis. We performed multiple alignments based on nucleic acid sequences of PAR-2 from various animals available from public databases, and designed primers for use in cloning of the Atlantic salmon PAR2 transcript. We further cloned and characterized the full length sequence of Atlantic salmon PAR2 and investigated the expression in both early and chronic stages of SBM induced enteropathy. Two full length versions of PAR-2 cDNA were identified and termed PAR-2a and PAR-2b. Expression of the two PAR-2 transcripts was detected in all 18 tissues examined, but most extensively in the intestine and gills. A significant up-regulation in the distal intestine was observed for the PAR-2a transcript after 1 day feeding diets containing SBM. After 3 weeks of feeding, PAR2a was down-regulated compared to the fish fed control diets. These findings may indicate that PAR-2a participates in inflammatory responses in both the early and later stages of the SBM enteropathy. In the chronic stages of the enteropathy, down-regulation of PAR-2a may indicate a possible desensitization of the PAR-2a receptor. Expression of PAR-2b was not altered in the first 7 days of SBM feeding, but a significant up regulation was observed after 3 weeks, suggesting a putative role in chronic stages of SBM induced enteritis. The expression differences of the two PAR-2 transcripts in the feed trials may indicate that they have different roles in the SBM induced enteritis. - Introduction By ingesting feed, the gastrointestinal tract (GI tract) is presented to food components and microorganisms carried with the feed exposing the organism to allergens and pathogens that can cause disease and hence affect animal welfare. The GI tract is one of a few major entry points for microorganisms and pathogens, and hence, animals have well developed physical and chemical barriers in combination with an effective mucosal immune system [1]. The mucosal and chemical barriers can be breached by microorganisms and pathogens, and once breached, circulating innate immune cells will form a second important basis for defence. The gut immune apparatus represents therefore a major element in the defence of an animal, and is considered the largest immunological organ in man [2]. A well functioning immune apparatus in the gastrointestinal tract is therefore of utmost importance for the function and wellbeing of all animals. SBM induced enteritis Standard qualities of soybean meal (SBM), the most important and cheapest protein rich feedstuff on the world market, can only be used at limited levels in salmonid diets because it challenges the gut immune systems and fish health. Salmon, when fed diets with standard qualities of SBM develop an inflammation like condition (enteritis) in the distal intestine characterized by inflammatory infiltrate in the intestinal mucosa, atrophy of primary and secondary mucosal folds and decrease of epithelial vacuolization [3-5]. A previous study investigating the development of the enteritis using a 33% SBM feed showed minor changes in intestinal histology in some of the samples after two days of feeding [3]. After 7 days of feeding SBM the fish displayed all the signs present in the fully developed condition including increased width and marked reduction in height of simple mucosal folds as well as increased cell infiltration of the lamina propria [3]. Further, upon feeding SBM for 1421 days the enteritis was fully developed in all the fish examined [3]. The SBM induced enteritis may also be a key factor in the decrease in growth as well as nutrient digestibility and absorption observed at higher inclusion levels [6-9] and has been suggested to have a negative effect on disease resistance [10]. Salmonids fed diets with standard qualities of SBM show elevated activity of trypsin-like enzymes [11,12] suggesting that trypsin might be involved in the development of SBM induced enteritis. Further, studies with partly fractionated soybean extracts have shown that the feed substances participating in the enteropathy in salmon are soluble in alcohol [13,14]. Later studies has suggested that saponins, present in the soy alcohol extract, is a compound that may cause some, but not all, of the intestinal alterations seen in Atlantic salmon fed soybean meal [15]. Saponins are known to increase permeability of intestinal tissue and thereby increase exposure to immune stimulants [16]. But still, more than 18 years after the SBM induced enteritis was reported, the causative molecular agents present in SBM responsible for the pathogenesis have not been identified. PAR-2 receptors and inflammation Several studies in mammalian species have shown that activation of cell surface receptors termed proteinase-activated receptors (PARs) are key activators of inflammatory responses in a wide range of tissues including the gastrointestinal tract (GI) and airways [17-23]. These cell surface receptors are G-protein coupled and belongs to a family of seven transmembrane receptors that can be activated by serine proteinases, such as trypsin. So far, four proteinaseactivated receptors have been cloned and studied in man [24-27], but to our knowledge no proteinase-activated receptors have been studied in teleost fish. Activation of PAR-2 (proteinase-activated receptor 2) in mammals is achieved by proteolytic cleavage of an extracellular peptide sequence hereby exposing an N-terminal tethered ligand domain that binds to and activates the receptor [28,29]. Upon activation of the PAR-2 receptor in mammals, the receptor is internalized and targeted to lysosomes for degradation [30,31]. To resensitize the cells from the irreversible receptor cleavage new receptors are mobilized by large Golgi stores as well as synthesis of new receptors [30]. The PAR-2 receptor has been detected in various diverse tissues such as brain, eye, airway, heart, GI tract, pancreas, kidney, liver, prostate, skin and in cells such as epithelial cells, endothelial cells, as well as in immune cells such as T-cells, neutrophils, mast cells and eosinophils [32,33]. Some of the PAR-2 mediated effects on leukocytes involve leukocyte rolling and adhesion [34] as well as leukocyte migration in vivo [35]. Activation of PAR-2 by serine proteinases have been shown, in vitro, to stimulate bone marrow progenitor cells to develop into dendritic cells [36]. Hence, serine proteinases might participate in adaptive immune responses in vivo. Activation of PAR-2 by trypsin in luminal colonocytes in mice affect the permeability and hence could play an important role in pathogenesis of different mammalian gastrointestinal disorders [22]. In humans, elevated colonic luminal serine proteinase activity of irritable bowels syndrome (IBS) patients has been suggested to involve PAR-2 activation and mediate epithelial barrier dysfunction and pathogenesis of IBS [37]. Mechanisms of immune responses in fish, whether stimulated by dietary components or pathogens are not well described. A better understanding of these responses is expected to lead the way to develop healthier and more productive diets and found the basis for improvements in disease prevention and treatments. The reported importance of PAR-2 activation in mammalian inflammatory diseases motivated the cloning, sequencing and expression analysis of the PAR-2 transcript in Atlantic salmon fed SBM diets. The data presented indicates a possible role of PAR-2 as a mediator of inflammatory responses in the distal intestine of Atlantic salmon. Materials and methods Experimental animals In order to study the impact of diets containing SBM on the mRNA expression of PAR-2, samples from both a Short term trial Farmed Atlantic salmon (Salmobreed strain), weighing 214 g (average) on the termination of the experiment, were kept in fibreglass tanks (1 1 0.6 m, water depth 4050 cm) containing running seawater (salinity 3234 g L-1) under 24 h light conditions. The water temperature was between 810C during the experimental period. During the experiment the fish were fed either a fishmeal (FM) diet or a diet containing 46% SBM (Table 1) for 1, 3 or 7 days. Prior to the feeding trial the fish were allocated in the fibreglass tanks and fed the fish meal diet for 27 days. Further details on formulation, chemical composition and production of the diets are given in [38]. The experiment was done at AKVAFORSK, The Institute of Aquaculture Research in Sunndalsra (Norway). Long term trial Farmed Atlantic salmon with an initial weight of approximately 176 g were distributed into fibreglass tanks (1 1 0.6 m, water depth 4050 cm) containing running sea water (5.6C). The fish were fed either a FM diet or a diet containing 30% SBM for 3 weeks (Table 1). Before the start of the trial the salmon were fed a commercial diet (Skretting AS, Stavanger, Norway). Further details on formulation and chemical composition of the diets as well as fish and rearing conditions are given in [12]. The experiment was done at AKVAFORSK, The Institute of Aquaculture Research in Sunndalsra (Norway). 794.6a 111 87 7 aNorsECO (Egersund Sildeoljefabrikk AS, Egersund, Norway). bDeno-Soy F, soybean meal with hull that is hexane extracted and toasted (Denofa, Fredrikstad, Norway). cSkretting Australia (Cambridge, TAS, Australia). dExtruded soybean meal, Skretting Australia (Cambridge, TAS, Australia). Collection of tissue samples Fish were randomly selected, anesthetized in tricaine methansulphate (MS222), weighed, measured and killed with a sharp blow to the head followed by abdominal evisceration. The intestines were cleaned of all fatty tissue and intestinal content prior to collection of samples. The intestinal regions were defined as follows: the pyloric intestine (PI) included the intestine with the caeca; the mid intestine (MI) included the intestine between the most distal pyloric caecum and the appearance of transverse folds of the luminal surface and the increase in intestinal diameter; the distal intestine (DI) included the intestine between the distal end of the MI and anus. For characterization of PAR-2a and PAR-2b mRNA expression in various tissues 300 mg of the following tissues were sampled from one fish fed a FM based diet: oesophagus, stomach, pancreas, PI, MI, DI, liver, head kidney, kidney, heart, spleen, thymus, brain, eye, gill, gonads, muscle and skin. All samples except for pancreas were stored in RNAlater (Ambion Inc.) at -20C until RNA isolation. Pancreas was collected as follows: approximately 300 mg of pancreatic tissue, i.e. diffuse pancreas embedded in the fatty tissue surrounding the pyloric caeca, was gently scraped off with a spatula and immediately snap frozen in liquid nitrogen then transferred to ten times the volume of RNAlater-ICE (Ambion, Inc.) and stored at -80C until RNA isolation. For quantification of PAR-2 mRNA expression approximately 300 mg of the DI from the short-term and the long-term trial was collected and stored on RNAlater (Ambion Inc.) at -20C until RNA isolation. The following diets and points of time were collected from the shortterm trial: from the FM group (control fish) 2 fish were collected on day 1, 3 and 7 respectively (6 in total); from the SBM group 6 fish were collected at day 1, 3 and 7 respectively (6 fish per day). Nine DI samples were collected from the FM and SBM group respectively in the long-term trial. Total RNA extraction Total RNA was isolated from oesophagus, stomach, pancreas, PI, MI, DI, liver, head kidney, kidney, heart, spleen, thymus, brain, eye, gill, gonads, and muscle using Trizol (Invitrogen Ltd, Paisley, UK) according to the manufacturer's protocol. A modified protocol was used for pancreas with three times the volume of reagents. First strand cDNA synthesis cDNA was generated from five microgram of total RNA using PowerScript Reverse Transcriptase (BD Biosciences, Franklin Lakes, NJ, USA) according to the manufacturer's protocol, primed with a mixture of oligo dT (25 ng/l) and random hexamer primers (2.5 ng/l). Cloning and sequencing of PAR-2 mRNA sequences Multiple DNA sequence alignments was performed from Homo sapiens [GenBank:NM_005242], Danio rerio [GenBank:XM_678622], Hippoglossus hippoglossus [GenBank:EB034068], Oncorhynchus mykiss [GenBank:BX861951] and Xenopus laevis [GenBank:BX850546] PAR-2 sequences using the publicly available web browser based bl2seq (Blast 2 Sequences, [39]). From these alignments we manually designed one degenerated (PAR-2R_Deg) and one regular PCR primer (PAR-2F) based on the identification of potential nucleic acid conservation of PAR-2 sequences. All PCR products amplified with Advantage 2 PCR enzyme mix (Clontech, Takara Bio Inc, Shiga, Japan) were used in a post-amplification procedure with addition of 2 U of Taq polymerase (Biotools, B&M Labs, Madrid, Spain) in 1 PCR buffer (Biotools) for 15 min at 72C before use in TOPO TA cloning (TOPO TA Cloning Kit; Invitrogen, Carlsbad, CA, USA). The PAR-2 primers and cDNA generated from the distal intestine were used in PCR amplification using Advantage 2 PCR enzyme mix (Clontech) in a total reaction volume of 25 l with the following cycling parameters: 35 cycles of 94C for 30 s, 60C for 30 s, and 72C for 30 s. A positive PCR product of 149 bp was cloned using TOPO TA Cloning kit (Invitrogen) according to the manufacturers' instructions. From the cloning, five clones were selected and grown for 16 h at 37C in Luria-Bertani media containing 50 g/ml ampicilin. Plasmid DNA was isolated (E.Z.N.A plasmid miniprep kit I, OMEGA BioTek, Inc, GA, USA) and sent for sequencing (GATC Biotech, Konstanz, Germany). From the PAR-2 sequence obtained we manually designed specific PCR primers unique for each PAR-2 versions for use in 5' and 3' RACE (rapid amplification of cDNA ends). mRNA was isolated from total RNA following the manufacturers instructions (MicroPoly(A)Purist Kit, Ambion, Austin, TX, USA), and approximately 1 g of was used for reverse transcription using SMART RACE cDNA amplification Kit (Clontech). The PCR reactions were performed using the Advantage 2 PCR enzyme mix (Clontech) with the following touchdown PCR setup; 3 min at 94C followed by: (30 s at 94C, 3 min at 72C) five cycles, (30 s at 94C, 30 s at 70C, 3 min at 72C) five cycles, (30 s at 94C, 30 s at 68C, 3 min at 72C) 32 cycles. From each transformation, 8 clones were selected and grown for 16 h at 37C in Luria-Bertani media containing 50 g/ml ampicilin, plasmids were isolated (E.Z.N.A plasmid miniprep Kit I) and sequenced (GATC Biotech). The sequence chromatograms were imported to the free software ContigExpress (Vector NTI Advance 10, Invitrogen), trimmed for vector and RACE primer sequences and assembled into contigs. Quantitative real-time RT-PCR Total RNA was extracted from DI as previously described. Prior to reverse transcription, total RNA from all samples were subjected to DNase treatment using a TURBO DNAfree kit (Ambion) in accordance with the manufacturer's recommendations. First strand cDNA synthesis was performed with 0.8 g total RNA from each sample using Superscript III (Invitrogen) and Oligo(dT)20 primers (Invitrogen) in accordance with the manufacturer's instructions. Real-time RT-PCR primers for the two PAR-2 transcripts were designed based on the full-length sequence using the free available software FastPCR [40]. Real-time RT-PCR primers for the housekeeping genes were designed using Primer3 software [41]. PCR reactions were performed in a total volume of 10 l using the LightCycler FastStart DNA MasterPLUS SYBR GREEN I kit (Roche Diagnostics) using 4.5 l PCR-grade water, 0.5 l of each PCR primer (10 M), 2.5 l (6.25 ng) cDNA template and 2 l master mix. The following program was used: Denaturation (10 min at 95C), amplification and quantification program repeated 40 times (10 sec. at 95C, 15 sec. at the appropriate annealing temperature for the gene specific primers (Table 2) and 10 sec. at 72C with a single fluorescence measurement), melting curve program (60C to 99C with a heating rate of 0.1C/sec.) and cooling program down to 40C. For determination of the crossing point (CP) the "second derivative maximum method" measuring maximum increase rate of newly synthesized DNA per cycle was used on the basis of the LightCycler software 4.0 (Roche Diagnostics). To confirm amplification specificity the PCR products from each primer pair were subjected to melting curve analysis and manual inspection of PCR products after each run by agarose gel electrophoresis. Relative quantification analyzes The relative expression ratio of target mRNAs was calculated using the LightCycler software 4.0 (Roche Diagnostics) with calibrator-normalized relative quantification and PCR efficiency correction based on a linear regression fit. RNA from tissues of a fish from the FM group was used as calibrator. Four reference genes; Elongation factor 1 alpha, Glyceraldehyde-3-phosphate dehydrogenase, 18S RNA and -actin (Table 2) were analyzed for stability of expression in the samples intended for relative quantification analyses using the geNorm software [42]. Relative standard curves were generated on the basis of cDNA pooled from 2 samples from each diet and sample time, diluted in 5-fold or 10-fold dilution steps to cover the expected detection range of the target and housekeeping genes. Gene name Accession number Primer name AF321836 AF321836 AF012125 AF012126 BU693999 BU693999 AJ427629 AJ427629 PAR-2F ATCTACATGGCCAACCTGGC PAR-2R_Deg CAGTACAYGTTCCCGTAGAAGAA PAR-2a_RACE_F1 GTCCGACCTGCTCTTTGTCATCTGGA PAR-2a_RACE_R1 TGGACTCCCCTGAAGATTGCCTACCAC PAR-2b_RACE_F1 CTGGACACCTCTGAAGATCGCCTACCAC PAR-2b_RACE_R1 GCCCACCAGGACTTTACACAGCCT PAR-2a_RT_F1 GCGCTACTGTGCCATCGTCAA PAR-2a_RT_R1 TGGTCATCAGCCAGACCCCCA PAR-2b_RT_F1 ACGCTACTGGGGTGTGGCCCA PAR-2b_RT_R1 TGGTGGTGAGCCAGATGAAGG SS-EF1-alpha F1 GTGCTGTGCTTATCGTTGCT SS-EF1-alpha R1 GGCTCTGTGGAGTCCATCTT SS beta-aktin F1 CAAAGCCAACAGGGAGAAGATGA SS beta-aktin R1 ACCGGAGTCCATGACGATAC GAPDH F1 AAGTGAAGCAGGAGGGTGGAA GAPDH R1 CAGCCTCACCCCATTTGATG SS-18SrRNA F1 TACAGTGAAACTGCGAATGG SS-18SrRNA R1 GCATGGGTTTTGGGTCTG PAR-2: Proteinase-activated receptor-2, ELF-1 : Elongation factor 1 alpha, GAPDH: Glyceraldehyde-3-phosphate dehydrogenase. * PCR product size after RACE amplification Phylogenetic analyzes To examine the evolutionary relationship of the cloned Atlantic salmon sequences we aligned them with published PAR-2 sequences from a set of animal species using MEGA 4 [43], and used Jalview [44] to visualize the aligned sequences. The following sequences were used; human [GenBank:NM_001992, GenBank:NM_005242, GenBank:NM_004101, GenBank:NM_003950], dog [GenBank:XM_546059, GenBank:XM_546057, GenBank:XM_844773, GenBank:XM_541962] mouse [GenBank:NM_010169, GenBank:NM_007974, GenBank:NM_010170, GenBank:NM_007975], rat [GenBank:NM_012950, GenBank:NM_053897, GenBank:NM_053313, GenBank:NM_053808], zebrafish [GenBank:XM_694943], frog [GenBank:NM_001085783, GenBank:NM_001086070]. In MEGA 4 we produced a cladogram using Neighbor-joining with the following settings; bootstrap = 10000 seed = 38877, complete deletion for gaps/missing data, Poisson correction for amino acid substitution and uniform rates among sites. Statistics The Shapiro-Wilk W test was used to test conformity with the normal distribution. Student's t test was used to compare the relative expression of the respective genes between diets in the three week feed trial. To correct for multi comparisons of means, analysis of variance was followed by Tukey's Honestly Significant Difference (HSD) test. All results are presented as mean values with bars representing SEM. All tests were carried out two-tailed, with a significance level of 5%. The statistical analyses were performed using JMP 5.0.1 software package (SAS Institute Inc. Cary, NC, USA). Results and discussion Full-length cloning of PAR-2 transcripts Using cloning and sequencing we identified two fulllength PAR-2 mRNA transcripts termed PAR-2a [GenBank:FJ184031] and PAR-2b [GenBank:FJ184032] respectively, with 78% nucleic acid identity to each other in the deduced open reading frame (ORF). The large difference between the two transcripts indicate that they are likely to be derived from two genes, probably caused by divergence of the duplicated genome of Atlantic salmon [45]. Several expressed genes have previously been shown to be duplicated in Atlantic salmon [46-49], but little is known about what fraction of the reported duplicated genes are functional. Deduced amino acid similarities of the two PAR-2 receptors were compared to other species by performed multiple alignments with known PAR-2 sequences from Homo sapiens and Danio rerio (Figure 1). To visualize the phylogenetic sequence relations we produced a cladogram using Neighbour joining with BLOSUM62 matrix (Figure 2). From the alignments and the cladogram, both deduced Atlantic salmon PAR-2 sequences show similarity to other known PAR-2 sequences. However, the PAR2a sequence show more similarity to Danio rerio PAR-2 sequence than to the alternative Atlantic salmon PAR-2b sequence, which may indicate PAR-2a as the putative ancestral gene. We also observed considerable differences in the first 145 and 200229 aa (amino acids) of the two deduced Atlantic salmon PAR-2 proteins. These regions of the protein represent the putative N-terminal domain and extra cellular loop 2 region, both being essential in the activation of this receptor in mammals. Both deduced Atlantic salmon PAR-2 protein sequences have a serine proteinase cleavage site in the N-terminal part of the protein (Figure 1) in a comparable position of the serine proteinase site in the human PAR-2 protein. Expression studies of PAR-2 transcripts Thorough testing of the PCR primer pairs with diluted plasmid templates for both PAR-2 genes in real-time RTPCR experiments showed high specificity for the two PAR2 transcripts (data not shown). Expression of both PAR-2 transcripts was seen in all the tissues examined, with about 10100 fold higher expression in gills, pyloric-, mid-, and distal intestine (Figure 3). Our findings are similar to reports of high expression of PAR-2 in the colon and small intestine of man [25,50]. In man, PAR-2 has been demonstrated to mediate infiltration of leukocytes as well as hyperreactivity in allergic inflammation of the airway [23]. Hence, the high expression of the PAR-2 receptor transcripts observed in the gills of Atlantic salmon could point to analogous functions in the respiratory organ of fishes. Transcription level for PAR-2a showed a rapid and significant up-regulation at day 1 in fish fed diet with SBM whereas no expression differences was seen at day 3 or 7 (Figure 4). For PAR-2b there was no significant expression change during the first 7 days in fish fed diets containing SBM (Figure 4). Histopathological changes in the distal intestine of the fish fed the SBM diets showed similar features as previously described in another study [3]. No histopathological changes were observed after one day of feeding SBM, and only minor changes in some fish were seen at day three (results not shown). However, after 7 days most fish displayed the features of SBM induced enteritis (results not shown). Most fish fed SBM diet for 21 aFMniugdluthirpuelme1anlig(nHmse)n[tGseonfBdaendku:NceMd_P0A0R52-242a]mviinsouaaliczieddseuqsiunegnJcaelvsiefrwo m[44A]tlantic salmon (Ss), zebrafish (Dr) [GenBank:XM_694943] Multiple alignments of deduced PAR-2 amino acid sequences from Atlantic salmon (Ss), zebrafish (Dr) [GenBank:XM_694943] and human (Hs) [GenBank:NM_005242] visualized using Jalview 44[44]. The following percentage amino acid identity between the compared sequences is indicated with blue color, <40% identity has no color, >40% is light blue, >60% is medium dark blue, and >80% is dark blue. itFAaoirgrcislu)1a,rdmeo4go2(ruPasAmeR(s-M1h,ouPwsAminRgu-s2tch,uePluArse)R,l-ar3taitoan(RdshaPtiptAuosRfn-4Ao)rtvleagnitciucss),alzmebornaf(iSshal m(Doasnaiolare,rinio)readndleftrtoegrs()X,ehnuompauns l(aHeovims)opsraoptieinnsa)s,ed-oagct(ivCaatneids fraemceil-pA cladogram showing the relationship of Atlantic salmon (Salmo salar, in red letters), human (Homo sapiens), dog (Canis familiaris), mouse (Mus musculus), rat (Rattus norvegicus), zebrafish (Danio rerio) and frog (Xenopus laevis) proteinase-activated receptor 14 (PAR-1, PAR-2, PAR-3 and PAR-4). The cladogram was created in MEGA 4 [43] using Neighbor-joining (BLOSUM62 matrix). days displayed fully developed enteritis after histological investigations as been reported previously [12]. Interestingly, at three weeks feeding a diet containing SBM, a significant down-regulation was seen for PAR-2a, and a significant up-regulation was detected for PAR-2b compared to fish fed the control diet. Even though many duplicated genes in Atlantic salmon might be classified as pseudogenes, the observed response for the two PAR-2 genes may suggest involvement of both genes in the SBM induced enteritis. Overexpression of PAR-2 has been RFeiglautrivee3expression of PAR-2a (A) and PAR-2b (B) respectively Relative expression of PAR-2a (A) and PAR-2b (B) respectively. Expression levels are relative to muscle tissue samples. The following tissues were examined; ST: stomach, GI: gills, LI: liver, ES: esophagus, PI: pyloric caeca, MI: mid intestine, DI: distal intestine, HK: head-kidney, KI: kidney, SK: skin, MU: muscle, GO: gonads, PA: pancreas, EY: eye, BR: brain, TH: thymus, SP: spleen, HE: heart. RFeiglautrivee4mRNA expression of PAR-2 in the distal intestine of Atlantic salmon Relative mRNA expression of PAR-2 in the distal intestine of Atlantic salmon. The gene expression was normalized to both elongation factor 1 and -actin, and an average normalized ratio for each individual was calculated. The x-axis represents days after introduction to SBM and the y-axis represents the normalized ratio. Relative expression of PAR-2a (A) and PAR-2b (B) in fish fed fishmeal (FM) (n = 6) day 0 or a diet with inclusion of soybean meal (SBM) at day 1 (n = 6), at day 3 (n = 6) and at day 7 (n = 6) days. Relative expression of PAR-2a (C) and PAR-2b (D) of fish fed FM diet and diet with inclusion of SBM after 3 weeks of feeding. Error bars indicate S.E.M (standard error of the mean). Different lower case letters denote significant differences (P < 0.05) between the means. observed in biopsies from IBD (inflammatory bowels disease) patients and PAR-2 could play an important role in the development of colonic inflammation in man [20]. A pro-inflammatory role for PAR-2 was first shown in the colon of mice where acute PAR-2 activation led to an increase in epithelial permeability and bacterial translocation [17]. Patients with ulcerative colitis treated with a tryptase inhibitor shown relieved symptoms or remission of the disease [51], suggesting involvement of PAR-2 and tryptase in the gastrointestinal disease in these patients. Luminal proteinases has been demonstrated to regulate colonic paracellular permeability in mice, and that bacterial flora influences the degranulation of mucosal mast cells [22]. Further, it was suggested that the increased expression of PAR-2 observed in colonocytes is influenced by increased luminal proteinase activity rather than the release of proteinases such as tryptase [22]. Increased luminal trypsin-like activity in the distal intestine of Atlantic salmon fed diets with SBM [11,12] may suggest a similar mode of PAR-2 activation by serine proteases in fish. In mouse colon and small intestine a higher expression of PAR-2 has been reported in the surface epithelial cells lining the upper two thirds of the villi compared to cells located in the crypt region [50]. An increased proliferative compartment length as well as lower mucosal fold height have been reported in fish fed SBM feed for three weeks or more [52]. As a consequence, the observed reduction of PAR-2a expression after 3 weeks on a SBM diet may be caused by a reduced number of proliferated cells expressing PAR-2 towards the tip of the villi compared to the control fish. If the two PAR-2 transcripts in Atlantic salmon are not expressed equally in the same cell populations, the increased expression of PAR-2b in fish fed SBM feed for 3 weeks might be caused by the number of proliferated cells or the reported leukocyte infiltrate in fish fed diets containing SBM. The expression profile of the two transcripts in different cell populations of the intestine needs to be further investigated. Recent studies have shown that inflammation of the gut disrupts the normal microbiota and dramatically boost colonization of pathogenic bacteria in man [53,54]. An inflamed gut is therefore an open invitation to several species of pathogenic bacteria further promoting the inflammation and a factor in causing disease. The microbiota in Atlantic salmon changes upon exposure to SBM and a more diverse population of adherent bacteria has been reported after 3 weeks feeding a diet containing SBM [52]. The change of PAR-2a expression detected after one day of exposure to feed containing SBM suggest PAR-2 receptor activation and could therefore be responsible for an initiation of inflammation. Such an inflammation in concert with the new feed components could allow colonization of new bacteria and ultimately changing the normal microbiota. It is not known however if the microbiota of Atlantic salmon fed feed containing SBM is altered as early as one day after feeding, and a putative involvement of bacteria in the early phases of the development of enteritis merits further investigation. The putative role PAR-2 seems to have in intestinal inflammation in fishes makes it a potential important marker for enteritis. Soybean meal appears as a promising tool for studies of PAR-2, intestinal inflammation responses as well as intestinal cell populations in Atlantic salmon. Conclusion In our study we have demonstrated that Atlantic salmon have putative duplicated gene versions of the PAR-2 receptor. Both transcripts are highly expressed in the gastrointestinal tract and the gills. In Atlantic salmon fed inclusion levels of SBM the expression of the two PAR-2 transcripts is altered. We have shown that PAR-2a has a significant change of expression after one day of feeding diets containing SBM, but that the expression is significantly decreased in a three week feeding trial. The expression of PAR-2b did not show altered expression in the first seven days of feeding but a significant increase in expression is observed after three weeks. The altered expression of the two PAR-2 transcripts in the gut of fish fed diets containing SBM suggests that PAR-2 may have an important role in inflammation in fishes and other lower vertebrates. The identification of the PAR-2 genes in Atlantic salmon, a known initiator of inflammation in the gut of mammals, opens up for future studies to further shed light on molecular causes of the SBM induced enteritis observed in salmonids. Competing interests The authors declare that they have no competing interests. Authors' contributions JT planned the experiments, conducted the primer design, cloning of PAR-2 sequences, performed multiple alignment and phylogenetic analysis, participated in the realtime RT-PCR and drafted the manuscript. ECV isolated RNA, performed real-time RT-PCR and participated in the sampling. EL contributed in the real-time RT-PCR, in the sampling and in the drafting of the manuscript. K participated in the planning of the experiments, drafting of the manuscript and contributed to the intellectual content. 1. Dommett R , Zilbauer M , George JT , Bajaj-Elliott M : Innate immune defence in the human gastrointestinal tract . Mol Immunol 2005 , 42 : 903 - 912 . 2. Brandtzaeg P , Halstensen TS , Kett K , Krajci P , Kvale D , Rognum TO , Scott H , Sollid LM : Immunobiology and Immunopathology of Human Gut Mucosa - Humoral Immunity and Intraepithelial Lymphocytes . Gastroenterology 1989 , 97 : 1562 - 1584 . 3. Baeverfjord G , Krogdahl A : Development and regression of soybean meal induced enteritis in Atlantic salmon, Salmo salar L, distal intestine: A comparison with the intestines of fasted fish . J Fish Dis 1996 , 19 : 375 - 387 . 4. Ingh TSGA van den, Krogdahl A : Negative Effects of Antinutritional Factors from Soya Beans in Salmonids . Tijdschr Diergeneeskd 1990 , 115 : 935 - 938 . 5. Ingh TSGA van den, Krogdahl A , Olli JJ , Hendriks HGCJ , Koninkx JGJF : Effects of Soybean-Containing Diets on the Proximal and Distal Intestine in Atlantic Salmon (Salmo-Salar) - A Morphological-Study . Aquaculture 1991 , 94 : 297 - 305 . 6. Krogdahl A , Bakke-McKellep AM , Baeverfjord G : Effects of graded levels of standard soybean meal on intestinal structure, mucosal enzyme activities, and pancreatic response in Atlantic salmon ( Salmo salar L.). Aquacult Nutr 2003 , 9 : 361 - 371 . 7. Nordrum S , Bakke-McKellep AM , Krogdahl A , Buddington RK : Effects of soybean meal and salinity on intestinal transport of nutrients in Atlantic salmon (Salmo salar L.) and rainbow trout (Oncorhynchus mykiss) . Comp Biochem Phys B 2000 , 125 : 317 - 335 . 8. Olli JJ , Krogdahl A , Vandeningh TSGA , Brattas LE : Nutritive-Value of 4 Soybean Products in Diets for Atlantic Salmon (SalmoSalar , L). Acta Agr Scand A-An 1994 , 44 : 50 - 60 . 9. Olli JJ , Krogdahl A : Alcohol soluble components of soybeans seem to reduce digestibility in fish-meal-based diets for Atlantic salmon , Salmo salar L. Aquac Res 1995 , 26 : 831 - 835 . 10. Krogdahl A , Bakke-McKellep AM , Roed KH , Baeverfjord G : Feeding Atlantic salmon Salmo salar L. soybean products: effects on disease resistance (furunculosis), and lysozyme and IgM levels in the intestinal mucosa . Aquacult Nutr 2000 , 6 : 77 - 84 . 11. Lilleeng E , Froystad MK , Ostby GC , Valen EC , Krogdahl A : Effects of diets containing soybean meal on trypsin mRNA expression and activity in Atlantic salmon (Salmo salar L) . Comp Biochem Phys A 2007 , 147 : 25 - 36 . 12. Refstie S , Glencross B , Landsverk T , Sorensen M , Lilleeng E , Hawkins W , Krogdahl A : Digestive function and intestinal integrity in Atlantic salmon (Salmo salar) fed kernel meals and protein concentrates made from yellow or narrow-leafed lupins . Aquaculture 2006 , 261 : 1382 - 1395 . 13. Ingh TSGA van den, Olli JJ , Krogdahl A : Alcohol-soluble components in soybeans cause morphological changes in the distal intestine of Atlantic salmon , Salmo salar L. J Fish Dis 1996 , 19 : 47 - 53 . 14. Bureau DP , Harris AM , Cho CY : The effects of purified alcohol extracts from soy products on feed intake and growth of chinook salmon (Oncorhynchus tshawytscha) and rainbow trout (Oncorhynchus mykiss) . Aquaculture 1998 , 161 : 27 - 43 . 15. Knudsen D , Uran P , Arnous A , Koppe W , Frokiaer H : Saponin-containing subfractions of soybean molasses induce enteritis in the distal intestine of Atlantic salmon . J Agric Food Chem 2007 , 55 : 2261 - 2267 . 16. Johnson IT , Gee JM , Price K , Curl C , Fenwick GR : Influence of Saponins on Gut Permeability and Active Nutrient Transport Invitro . J Nutr 1986 , 116 : 2270 - 2277 . 17. Cenac N , Coelho AM , Nguyen C , Compton S , ndrade-Gordon P , MacNaughton WK , Wallace JL , Hollenberg MD , Bunnett NW , Garcia-Villar R , Bueno L , Vergnolle N : Induction of intestinal inflammation in mouse by activation of proteinase-activated receptor-2 . Am J Pathol 2002 , 161 : 1903 - 1915 . 18. Cenac N , Garcia-Villar R , Ferrier L , Larauche M , Vergnolle N , Bunnett NW , Coelho AM , Fioramonti J , Bueno L : Proteinase-activated receptor-2-induced colonic inflammation in mice: Possible involvement of afferent neurons, nitric oxide, and paracellular permeability . J Immunol 2003 , 170 : 4296 - 4300 . 19. Fiorucci S , Mencarelli A , Palazzetti B , Distrutti E , Vergnolle N , Hollenberg MD , Wallace JL , Morelli A , Cirino G : Proteinase-activated receptor 2 is an anti-inflammatory signal for colonic lamina propria lymphocytes in a mouse model of colitis . Proc Natl Acad Sci USA 2001 , 98 : 13936 - 13941 . 20. Kim JA , Choi SC , Yun KJ , Kim DK , Han MK , Seo GS , Yeom JJ , Kim TH , Nah YH , Lee YM : Expression of protease-activated receptor 2 in ulcerative colitis . Inflamm Bowel Dis 2003 , 9 : 224 - 229 . 21. Nystedt S , Ramakrishnan V , Sundelin J : The proteinase-activated receptor 2 is induced by inflammatory mediators in human endothelial cells - Comparison with the thrombin receptor . J Biol Chem 1996 , 271 : 14910 - 14915 . 22. Roka R , Demaude J , Cenac N , Ferrier L , Salvador-Cartier C , GarciaVillar R , Fioramonti J , Bueno L : Colonic luminal proteases activate colonocyte proteinase-activated receptor-2 and regulate paracellular permeability in mice . Neurogastroenterol Motil 2007 , 19 : 57 - 65 . 23. Schmidlin F , Amadesi S , Dabbagh K , Lewis DE , Knott P , Bunnett NW , Gater PR , Geppetti P , Bertrand C , Stevens ME : Protease-activated receptor 2 mediates eosinophil infiltration and hyperreactivity in allergic inflammation of the airway . J Immunol 2002 , 169 : 5315 - 5321 . 24. Ishihara H , Connolly AJ , Zeng DW , Kahn ML , Zheng YW , Timmons C , Tram T , Coughlin SR : Protease-activated receptor 3 is a second thrombin receptor in humans . Nature 1997 , 386 : 502 - 506 . 25. Nystedt S , Emilsson K , Larsson AK , Strombeck B , Sundelin J : Molecular-Cloning and Functional Expression of the Gene Encoding the Human Proteinase-Activated Receptor-2 . Eur J Biochem 1995 , 232 : 84 - 89 . 26. Vu TKH , Hung DT , Wheaton VI , Coughlin SR : Molecular-Cloning of A Functional Thrombin Receptor Reveals A Novel Proteolytic Mechanism of Receptor Activation . Cell 1991 , 64 : 1057 - 1068 . 27. Xu WF , Andersen H , Whitmore TE , Presnell SR , Yee DP , Ching A , Gilbert T , Davie EW , Foster DC : Cloning and characterization of human protease-activated receptor 4 . Proc Natl Acad Sci USA 1998 , 95 : 6642 - 6646 . 28. Nystedt S , Emilsson IE , Wahlestedt C , Sundelin J : Molecular-Cloning of A Potential Proteinase Activated Receptor . Proc Natl Acad Sci USA 1994 , 91 : 9208 - 9212 . 29. Nystedt S , Larsson AK , Aberg H , Sundelin J : The Mouse Proteinase-Activated Receptor-2 Cdna and Gene - Molecular-Cloning and Functional Expression . J Biol Chem 1995 , 270 : 5950 - 5955 . 30. Bohm SK , Khitin LM , Grady EF , Aponte G , Payan DG , Bunnett NW : Mechanisms of desensitization and resensitization of proteinase-activated receptor-2 . J Biol Chem 1996 , 271 : 22003 - 22016 . 31. Jacob C , Cottrell GS , Gehringer D , Schmidlin F , Grady EF , Bunnett NW: c-Cbl mediates ubiquitination, degradation, and downregulation of human protease-activated receptor 2 . J Biol Chem 2005 , 280 : 16076 - 16087 . 32. Macfarlane SR , Seatter MJ , Kanke T , Hunter GD , Plevin R : Proteinase-activated receptors. Pharmacol Rev 2001 , 53 : 245 - 282 . 33. Steinhoff M , Buddenkotte J , Shpacovitch V , Rattenholl A , Moormann C , Vergnolle N , Luger TA , Hollenberg MD : Proteinase-activated receptors: Transducers of proteinase-mediated signaling in inflammation and immune response . Endocr Rev 2005 , 26 : 1 - 43 . 34. Lindner JR , Kahn ML , Coughlin SR , Sambrano GR , Schauble E , Bernstein D , Foy D , Hafezi-Moghadam A , Ley K : Delayed onset of inflammation in protease-activated receptor-2-deficient mice . J Immunol 2000 , 165 : 6504 - 6510 . 35. Vergnolle N : Proteinase-activated receptor-2-activating peptides induce leukocyte rolling, adhesion, and extravasation in vivo . J Immunol 1999 , 163 : 5064 - 5069 . 36. Fields RC , Schoenecker JG , Hart JP , Hoffman MR , Pizzo SV , Lawson JH : Protease-activated receptor-2 signaling triggers dendritic cell development . Am J Pathol 2003 , 162 : 1817 - 1822 . 37. Gecse K , Roka R , Ferrier L , Leveque M , Eutamene H , Cartier C , itBelgnaoui A , Rosztoczy A , Izbeki F , Fioramonti J , Wittmann T , Bueno L : Increased faecal serine protease activity in diarrhoeic IBS patients: a colonic lumenal factor impairing colonic permeability and sensitivity . Gut 2008 , 57 : 591 - 598 . 38. Kraugerud OF , Penn M , Storebakken T , Refstie S , Krogdahl A , Svihus B : Nutrient digestibilities and gut function in Atlantic salmon (Salmo salar) fed diets with cellulose or non-starch polysaccharides from soy . Aquaculture 2007 , 273 : 96 - 107 . 39. Tatusova TA , Madden TL : BLAST 2 SEQUENCES, a new tool for comparing protein and nucleotide sequences . FEMS Microbiol Lett 1999 , 174 : 247 - 250 . 40. FastPCR [http://www.biocenter.helsinki.fi/bi/programs/fast pcr.htm] 41. Rozen S , Skaletsky HJ : Primer3 on the WWW for general users and for biologist programmers . In Methods Mol Biol Volume 132. Edited by: Krawetz S, Misener S. Totowa , NJ: Humana Press ; 2000 : 365 - 386 . 42. Vandesompele J , De Preter K , Pattyn F , Poppe B , Van Roy N , De Paepe A , Speleman F : Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes . Genome Biol 2002 , 3 : RESEARCH0034 . 43. Tamura K , Dudley J , Nei M , Kumar S : MEGA4: Molecular evolutionary genetics analysis (MEGA) software version 4 .0. Mol Biol Evol 2007 , 24 : 1596 - 1599 . 44. Clamp M , Cuff J , Searle SM , Barton GJ : The Jalview Java alignment editor . Bioinformatics 2004 , 20 : 426 - 427 . 45. Allendorf FW , Thorgaard GH : Tetraploidy and the evolution of Salmonid fishes . In Evolutionary genetics of fishes Edited by: Turner BJ. New York : Plenum Publishing Corporation ; 1984 : 1 - 53 . 46. Haugland O , Mercy IS , Romoren K , Torgersen J , Evensen O : Differential expression profiles and gene structure of two tumor necrosis factor-alpha variants in Atlantic salmon ( Salmo salar L.). Mol Immunol 2007 , 44 : 1652 - 1663 . 47. Ng SHS , Artieri CG , Bosdet IE , Chiu R , Danzmann RG , Davidson WS , Ferguson MM , Fjell CD , Hoyheim B , Jones SJM , de Jong PJ , Koop BF , Krzywinski MI , Lubieniecki K , Marra MA , Mitchell LA , Mathewson C , Osoegawa K , Parisotto SE , Phillips RB , Rise ML , von Schalburg KR , Schein JE , Shin HS , Siddiqui A , Thorsen J , Wye N , Yang G , Zhu BL : A physical map of the genome of Atlantic salmon, Salmo salar . Genomics 2005 , 86 : 396 - 404 . 48. Thorsen J , Zhu BL , Frengen E , Osoegawa K , de Jong PJ , Koop BF , Davidson WS , Hyheim B : A highly redundant BAC library of Atlantic salmon (Salmo salar): an important tool for salmon projects . BMC Genomics 2005 , 6 :. 49. Thorsen J , Hoyheim B , Koppang EO : Isolation of the Atlantic salmon tyrosinase gene family reveals heterogenous transcripts in a leukocyte cell line . Pigment Cell Res 2006 , 19 : 327 - 336 . 50. Bohm SK , Kong WY , Bromme D , Smeekens SP , Anderson DC , Connolly A , Kahn M , Nelken NA , Coughlin SR , Payan DG , Bunnett NW : Molecular cloning, expression and potential functions of the human proteinase-activated receptor-2 . Biochem J 1996 , 314 : 1009 - 1016 . 51. Tremaine WJ , Brzezinski A , Katz JA , Wolf DC , Fleming TJ , Mordenti J , Strenkoski-Nix LC , Kurth MC : Treatment of mildly to moderately active ulcerative colitis with a tryptase inhibitor (APC 2059): an open-label pilot study . Aliment Pharmacol Ther 2002 , 16 : 407 - 413 . 52. Bakke-McKellep AM , Penn MH , Salas PM , Refstie S , Sperstad S , Landsverk T , Ringo E , Krogdahl A : Effects of dietary soyabean meal, inulin and oxytetracycline on intestinal microbiota and epithelial cell stress, apoptosis and proliferation in the teleost Atlantic salmon ( Salmo salar L.). Br J Nutr 2007 , 97 : 699 - 713 . 53. Lupp C , Robertson ML , Wickham ME , Sekirov I , Champion OL , Gaynor EC , Finlay BB : Host-mediated inflammation disrupts the intestinal microbiota and promotes the Overgrowth of Enterobacteriaceae . Cell Host & Microbe 2007 , 2 : 119 - 129 . 54. Stecher B , Robbiani R , Walker AW , Westendorf AM , Barthel M , Kremer M , Chaffron S , Macpherson AJ , Buer J , Parkhill J , Dougan G , von Mering C , Hardt WD : Salmonella enterica serovar typhimurium exploits inflammation to compete with the intestinal microbiota . Plos Biology 2007 , 5 : 2177 - 2189 .


This is a preview of a remote PDF: http://www.journal-inflammation.com/content/pdf/1476-9255-5-18.pdf

Jim Thorsen, Einar Lilleeng, Elin Valen, Åshild Krogdahl. Proteinase-activated receptor-2: two potential inflammatory mediators of the gastrointestinal tract in Atlantic salmon, Journal of Inflammation, 2008, 18, DOI: 10.1186/1476-9255-5-18