Cerebrospinal Fluid Biomarker and Brain Biopsy Findings in Idiopathic Normal Pressure Hydrocephalus

PLOS ONE, Mar 2014

Background The significance of amyloid precursor protein (APP) and neuroinflammation in idiopathic normal pressure hydrocephalus (iNPH) and Alzheimer's disease (AD) is unknown. Objective To investigate the role of soluble APP (sAPP) and amyloid beta (Aβ) isoforms, proinflammatory cytokines, and biomarkers of neuronal damage in the cerebrospinal fluid (CSF) in relation to brain biopsy Aβ and hyperphosphorylated tau (HPτ) findings. Methods The study population comprised 102 patients with possible NPH with cortical brain biopsies, ventricular and lumbar CSF samples, and DNA available. The final clinical diagnoses were: 53 iNPH (91% shunt-responders), 26 AD (10 mixed iNPH+AD), and 23 others. Biopsy samples were immunostained against Aβ and HPτ. CSF levels of AD-related biomarkers (Aβ42, p-tau, total tau), non-AD-related Aβ isoforms (Aβ38, Aβ40), sAPP isoforms (sAPPα, sAPPβ), proinflammatory cytokines (several interleukins (IL), interferon-gamma, monocyte chemoattractant protein-1, tumor necrosis factor-alpha) and biomarkers of neuronal damage (neurofilament light and myelin basic protein) were measured. All patients were genotyped for APOE. Results Lumbar CSF levels of sAPPα were lower (p<0.05) in patients with shunt-responsive iNPH compared to non-iNPH patients. sAPPβ showed a similar trend (p = 0.06). CSF sAPP isoform levels showed no association to Aβ or HPτ in the brain biopsy. Quantified Aβ load in the brain biopsy showed a negative correlation with CSF levels of Aβ42 in ventricular (r = −0.295, p = 0.003) and lumbar (r = −0.356, p = 0.01) samples, while the levels of Aβ38 and Aβ40 showed no correlation. CSF levels of proinflammatory cytokines and biomarkers of neuronal damage did not associate to the brain biopsy findings, diagnosis, or shunt response. Higher lumbar/ventricular CSF IL-8 ratios (p<0.001) were seen in lumbar samples collected after ventriculostomy compared to the samples collected before the procedure. Conclusions The role of sAPP isoforms in iNPH seems to be independent from the amyloid cascade. No neuroinflammatory background was observed in iNPH or AD.

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Cerebrospinal Fluid Biomarker and Brain Biopsy Findings in Idiopathic Normal Pressure Hydrocephalus

et al. (2014) Cerebrospinal Fluid Biomarker and Brain Biopsy Findings in Idiopathic Normal Pressure Hydrocephalus. PLoS ONE 9(3): e91974. doi:10.1371/journal.pone.0091974 Cerebrospinal Fluid Biomarker and Brain Biopsy Findings in Idiopathic Normal Pressure Hydrocephalus Okko T. Pyykko 0 Miikka Lumela 0 Jaana Rummukainen 0 Ossi Nerg 0 Toni T. Seppa la 0 Massimo S. Fiandaca, Georgetown University Medical Center, United States of America 0 1 Neurosurgery of NeuroCenter, Kuopio University Hospital , Kuopio , Finland , 2 Neurology of NeuroCenter, Kuopio University Hospital , Kuopio , Finland , 3 Department of Neurology, Institute of Clinical Medicine, University of Eastern Finland , Kuopio , Finland , 4 Department of Pathology, Kuopio University Hospital , Kuopio , Finland , 5 Department of Immunology , Genetics and Pathology , Uppsala University , Uppsala , Sweden , 6 Clinical Neurochemistry Laboratory, Department of Psychiatry and Neurochemistry, The Sahlgrenska Academy, University of Gothenburg , Gothenburg , Sweden Background: The significance of amyloid precursor protein (APP) and neuroinflammation in idiopathic normal pressure hydrocephalus (iNPH) and Alzheimer's disease (AD) is unknown. Objective: To investigate the role of soluble APP (sAPP) and amyloid beta (Ab) isoforms, proinflammatory cytokines, and biomarkers of neuronal damage in the cerebrospinal fluid (CSF) in relation to brain biopsy Ab and hyperphosphorylated tau (HPt) findings. Methods: The study population comprised 102 patients with possible NPH with cortical brain biopsies, ventricular and lumbar CSF samples, and DNA available. The final clinical diagnoses were: 53 iNPH (91% shunt-responders), 26 AD (10 mixed iNPH+AD), and 23 others. Biopsy samples were immunostained against Ab and HPt. CSF levels of AD-related biomarkers (Ab42, p-tau, total tau), non-AD-related Ab isoforms (Ab38, Ab40), sAPP isoforms (sAPPa, sAPPb), proinflammatory cytokines (several interleukins (IL), interferon-gamma, monocyte chemoattractant protein-1, tumor necrosis factor-alpha) and biomarkers of neuronal damage (neurofilament light and myelin basic protein) were measured. All patients were genotyped for APOE. Results: Lumbar CSF levels of sAPPa were lower (p,0.05) in patients with shunt-responsive iNPH compared to non-iNPH patients. sAPPb showed a similar trend (p = 0.06). CSF sAPP isoform levels showed no association to Ab or HPt in the brain biopsy. Quantified Ab load in the brain biopsy showed a negative correlation with CSF levels of Ab42 in ventricular (r = 20.295, p = 0.003) and lumbar (r = 20.356, p = 0.01) samples, while the levels of Ab38 and Ab40 showed no correlation. CSF levels of proinflammatory cytokines and biomarkers of neuronal damage did not associate to the brain biopsy findings, diagnosis, or shunt response. Higher lumbar/ventricular CSF IL-8 ratios (p,0.001) were seen in lumbar samples collected after ventriculostomy compared to the samples collected before the procedure. Conclusions: The role of sAPP isoforms in iNPH seems to be independent from the amyloid cascade. No neuroinflammatory background was observed in iNPH or AD. - Funding: This study was funded by the Health Research Council of the Academy of Finland, The Finnish Medical Foundation, EVO/VTR grants from the Kuopio University Hospital 5252614, 5772708, the strategic funding from the University of Eastern Finland, and Finnish Cultural Foundations North Savo Regional Fund. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Competing Interests: The authors have declared that no competing interests exist. Idiopathic normal pressure hydrocephalus (iNPH) is a progressive neurodegenerative disorder of unknown etiology in the elderly presenting with gait disorder, cognitive impairment, and urinary incontinence, with enlarged ventricles of the brain but normal or slightly elevated cerebrospinal fluid (CSF) pressure [1,2]. Currently there is no pathological hallmark for iNPH [3]. Studies suggesting some potential genetic background of iNPH have been published [4,5]. The present treatment of choice in iNPH is CSF diversion with an implanted shunt that relieves or even reverses the symptoms. Various procedures to evaluate CSF dynamics in patients with possible iNPH are used to identify those who could benefit from CSF shunting. These include the CSF tap test, external lumbar drainage test, infusion tests, and intraventricular or intracranial pressure (ICP) monitoring [69]. The most frequent differential diagnoses of iNPH are atypical Alzheimers disease (AD) and vascular dementia [8,10]. AD is characterized by the hallmark lesions of amyloid-b (Ab) plaques and neurofibrillary tangles composed of hyperphosphorylated tau (HPt) in the brain of patients with amnestic cognitive decline [1113]. The amyloid cascade hypothesis states that Ab starts to accumulate decades before the clinical manifestations of AD [14,15]. In vivo, Ab can be detected directly with brain biopsy [8,16], or indirectly by observing low levels of Ab in CSF [17]. Fibrillar Ab can also be evaluated by positron emission tomography (PET) utilizing e.g. the 11C-labeled Pittsburgh compound B [18] or [18F]flutemetamol [19]. Although common pathways for iNPH and AD have been proposed [20], the findings in genetic [21] and Ab studies [8] suggest differences in etiologies of the two diseases. Ab and HPt in the CSF may help to differentiate iNPH and AD patient groups or detect comorbid AD in iNPH [17]. In addition, these biomarkers have shown a potency to predict response to shunt in iNPH [22,23]. Ab originates from a cell membrane-spanning protein, amyloid precursor protein (APP), which has diverse roles in normal neuronal function [24]. Soluble APP alpha (sAPPa) and beta (sAPPb) result from the cleavage of APP by a- and b-secretases, respectively. Low CSF levels of sAPP isoforms have been reported in post-stroke patients and iNPH compared to AD and normal healthy controls [2528]. In addition, sAPPa has shown a marked prognostic value for cognitive performance following shunt surgery [27], and subsequent increase of ventricular sAPP-levels has been noted in shunt-responders [28]. Abnormal levels of proinflammatory cytokines, such as interleukins (IL), interferon-gamma (IFN-c), monocyte chemoattractant protein-1 (MCP-1), and tumor necrosis factor-alpha (TNF-a), in CSF have been noted in various diseases of the nervous system, including AD [29]. In iNPH, several proinflammatory cytokines have been studied, but none of them has proven to be useful in diagnostics [30]. Lower levels of IL-1b in NPH compared to AD was reported in a single paper [31], while increased levels of IL-4 and IL-10 were reported in patients with NPH compared to healthy individuals in another study, but no significant difference was seen between NPH and other dementias [32]. No differences were found between NPH and AD or healthy controls in studies comparing the levels of IL-8, IL-10, IL-12 (p40 and p70), IFN-c, and transforming growth factor-b1 (TGF- b1) [28,33]. However, iNPH patients did show increased levels of MCP-1 compared to healthy individuals [28]. Prior to treatment, higher TNF-a concentrations (and subsequent normalization after shunting) in CSF were observed in NPH patients compared to healthy controls in a single study [34]; however, these results did not replicate in a more recent study with solely idiopathic NPH patients [35]. Elevated levels of neurofilament light protein (NFL) in the CSF, indicating neuronal death and axonal loss, have been found in iNPH and secondary NPH in several studies [28,3639]. Increased levels of myelin basic protein (MBP) in the CSF is a well-established biomarker for demyelination and myelin damage in the central nervous system [40]. Furthermore, elevated levels of MBP have been reported in NPH [41]. To our knowledge, studies assessing the association between proinflammatory cytokines and biomarkers of neuronal damage in CSF, and cortical brain biopsy have not been published to date. Objectives The objectives of the current study were: 1. to determine the levels of AD-related biomarkers (Ab42, ptau, total tau), non-AD-related Ab isoforms (Ab38, Ab40), sAPP isoforms (sAPPa, sAPPb), proinflammatory cytokines (IL 1b, 2, 4, 5, 8, 10, 12p70, and 13, IFN-c, MCP-1, TNF-a) and biomarkers of neuronal damage (NFL, MBP) in lumbar and ventricular CSF, and how they correlate, 2. study the relationship between the CSF biomarkers and cortical brain biopsy, 3. assess the diagnostic and prognostic value of the CSF biomarkers in iNPH and AD. Ethics statement This study was approved by the Kuopio University Hospital (KUH) Research Ethical Committee, The Finnish National Supervisory Authority for Welfare and Health, and The Finnish Ministry of Social Affairs and Health. All participants or their proxies gave a written, informed consent prior to participation in the study. If the clinician suspected dementia to significantly affect the capacity of the patient to consent, a next of kin, caretakers or guardians consented on the behalf of participants. When a consent was obtained from a participants proxy, the patients own opinion was inquired and considered, and no patients were recruited against their will. Kuopio NPH Registry and Protocol Neurosurgery of KUH solely provides full-time acute and elective neurosurgical services for the KUH catchment population in Middle and Eastern Finland. In addition, the KUH area contains four central hospitals with neurological units and catchment areas of their own [21]. Patients fulfilling the following criteria were further assessed in KUH Neurosurgery as possible NPH patients: (1) primary evaluation and examination by a neurologist indicating NPH; (2) one to three symptoms suggestive of NPH (gait disorder, cognitive impairment, urinary incontinence); and (3) NPH related brain imaging findings (enlarged ventricles (Evans index.0.3) together with obliterated cortical sulci). The diagnostic workup protocol of KUH Neurosurgery for possible NPH included a clinical examination, CT or MRI scan, and 24 h intraventricular ICP monitoring together with a frontal cortical brain biopsy. Kuopio NPH Registry (www.uef.fi/nph) consists of all evaluated possible NPH patients from the KUH catchment population since 1993 [21]. The ICP criteria for the shunt treatment in iNPH patients were (1) a basal ICP pressure between 10 and 20 mmHg continuously, or (2) the presence of A-waves or more than 30% B-waves during the 24 h monitoring, when basal pressure was between 5 and 10 mmHg [21]. Study population Altogether 102 patients, 51 men and 51 women, with a median age of 74.6 years (range 4787 years) from the Kuopio NPH Registry with cortical brain biopsy, APOE genotype, and a ventricular CSF sample available were included in the study (Table 1). 63 patients were diagnosed with iNPH according to the protocol above, and were shunted with ventriculoperitoneral shunt (PS Medical medium pressure or adjustable valve). The clinical response to shunt was evaluated at 23 months after surgery, and any subjective or objective improvement in patient gait, memory or urinary continence was graded as a positive shunt response. Clinical AD was diagnosed according to a protocol described earlier [8,10,21] in 26 patients (including 10 patients with initial/ primary diagnosis of iNPH) in a median follow-up time of 2.3 years (range 0.26.2 years). Age (years) (median (range)) Leading symptom, n (%) Immunoreactivity (n (%)) APOE-e4 carriers (n (%)) Follow-up time (years) (median (range)) Possible NPH (n = 102) Final clinical diagnosis of iNPH (n = 53) Shunt responder Shunt nonresponder Mixed iNPH + AD No diagnosis of iNPH (n = 39) Abreviations: APOE, apolipoprotein E gene; CSF, cerebrospinal fluid; NPH, normal pressure hydrocephalus; iNPH, idiopathic NPH; AD, Alzheimers disease; Ab, amyloid beta protein; HPt, hyperphosphorylated tau protein. doi:10.1371/journal.pone.0091974.t001 Immunohistochemistry and histological evaluation Biopsy samples representing frontal cortex and subcortical white matter were stained with hematoxylin-eosin and immunostained with monoclonal antibodies directed to Ab (6F/3D) and HPt (AT8) as described earlier in detail [8,21]. Positive Ab immunostain was further quantified and reported as ratio of area covered by Ab to total area of cortex in the biopsy as described earlier [17]. APOE genotyping DNA was extracted from venous blood using commercial kit according to manufacturers protocol (Illustra Blood GenomicPrep Mini Spin Kit, GE Healthcare, Little Chalfont, UK). A standard PCR method was used in the APOE genotyping [21,42]. CSF samples and biomarker analyses The ventricular CSF samples were collected immediately after the placement of intraventricular catheter (first 1 mL discarded) in the ICP measurement procedure. In addition, a lumbar CSF sample was available in 49 patients. The lumbar samples were obtained through a lumbar puncture prior to the ICP measurement protocol (12 patients) or 2448 hours after introducing the ventricular catheter (37 patients). Levels of AD biomarkers (Ab42, p-tau, total tau) were measured from the CSF samples using commercial ELISA kits (Innotest bamyloid142, Innotest Phosphotau(181P), Innotest Tau-Ag, Innogenetics, Ghent, Belgium) according to the manufacturers protocol at validated laboratory in Neurology (www.uef.fi/neuro), University of Eastern Finland, Kuopio, Finland as described earlier [17]. Ab isoforms (Ab38, Ab40), sAPP isoforms (sAPPa, sAPPb), and the proinflammatory cytokines (IL 1b, 2, 4, 5, 8, 10, 12p70, and 13, IFN-c, MCP-1, TNF-a) were analyzed utilizing commercially available multiplexed assays (Meso Scale Discovery, Gaithersburg, MD, USA) [28,43], and NFL and MBP concentrations were measured using commercial ELISA kits (NF-Light, UmanDiagnostics, Umea, Sweden, and ACTIVE MBP, Diagnostic Systems Laboratories, Webster, TX, USA, respectively). All analyses were performed according to the manufacturers protocols by boardcertified laboratory technicians at the Clinical Neurochemistry Laboratory, Sahlgrenska University Hospital, Molndal. Each set of biomarker measurements was performed on one day, using one batch of reagents. All clinical, immunohistochemical, and laboratory analyses were performed blinded to the result information of each other. Statistical analyses Nonparametric KruskalWallis H and MannWhitney U tests were used for comparing CSF levels of measured biomarkers between different groups, and the Wilcoxon signed-rank test for comparisons of two related samples. To define the correlation between different proinflammatory cytokines, Pearson correlation with Bonferroni correction (k = 55) was applied. Patients with tauopathy but no amyloid (n = 4) were excluded from the analyses of different biomarkers in relation to biopsy findings, and iNPH patients with co-morbid AD (n = 10) and iNPH patients with negative shunt-response (n = 5) from the analyses comparing true iNPH patients to non-iNPH patients. Some cytokines were below the lower limit of detection of the assays and graded as zero concentration in statistical analyses. One patient had an insufficient CSF sample for the analysis of sAPPs, and another for the analysis of Ab isoforms Ab38 and Ab40. IBM SPSS Statistics for Mac (version 19.0.0.2, IBM, Armonk, NY, USA) was used in the statistical analyses. The level of significance was set at p,0.05. Possible NPH (n = 102) Final clinical diagnosis of iNPH (n = 53) Shunt nonresponder Mixed iNPH + AD No diagnosis of iNPH (n = 39) Abreviations: CSF, cerebrospinal fluid; Ab42, amyloid beta 142; p-tau 181, tau phosphorylated at threonine 181; sAPP, soluable amyloid precursor protein; IL-8, interleukin 8; MCP-1, monocyte chemoattractant protein-1; TNF-a, tumor necrosis factor-alpha; NFL, neurofilament light protein; MBP, myelin basic protein. *Mean (SD) CSF concentrations in ng/L. doi:10.1371/journal.pone.0091974.t002 Ab and sAPP isoforms Lumbar CSF levels of sAPPa were significantly lower (p,0.05) in patients with iNPH and a positive shunt reponse compared to non-iNPH patients, while sAPPb showed a similar tendency (p = 0.06, Table 2, Figure 1B and 1D). However, no such association was seen for sAPP isoforms in ventricular CSF samples (p = 0.370.47, Table 2, Figure 1A and 1C). In iNPH patients, a tendency towards lower levels of sAPPa and sAPPb were observed (p = 0.230.49) in shunt-responders compared to nonresponders in ventricular and lumbar CSF (Table 2). Ventricular CSF levels of Ab42 differed (p = 0.003) between different brain biopsy groups (Table 3, Figure 2C). Patients with positive Ab and HPt immunoreactivity in the cortical brain biopsy showed significantly lower CSF levels of Ab42 compared to the Ab positive and HPt negative group (post hoc p = 0.008), and to the Ab and HPt negative group (post hoc p = 0.005, Table 3, Figure 2C). Similar associations were seen in lumbar samples (data not shown). Quantified Ab load showed a negative correlation with the levels of CSF Ab42 in ventricular (Pearsons r = 20.295, p = 0.003) and lumbar (Pearsons r = 20.356, p = 0.01) samples (Figure 3). However, the CSF levels of other Ab isoforms (Ab38, Ab40) and sAPP isoforms (sAPPa, sAPPb) did not correlate (p = 0.590.95) with the presence of Ab or HPt in the biopsy (Table 3, Figure 2AB and 2DE). Furthermore, there was no correlation between the levels of sAPP isoforms in the CSF and Ab load in cortical brain biopsy (p = 0.840.92). There were no statistically significant differences between the levels of CSF Ab or tau biomarkers in shunt-responding and nonresponding iNPH patients. Proinflammatory cytokines Several cytokines were present at concentrations below the lower limit of detection of the assay (Table S1). All tested proinflammatory cytokines showed positive correlations between each other in ventricular (Table S1) and lumbar (Table S2) CSF samples. All ventricular and lumbar CSF IL-8 samples and all but one ventricular CSF MCP-1 sample were above the lower limit of detection, and the two cytokines and TNF-a were chosen for further analyses from the proinflammatory cytokines measured. Lumbar CSF samples showed higher IL-8 levels compared to ventricular samples (p,0.001). Moreover, lumbar/ventricular CSF IL-8 ratios (p,0.001) were significantly higher in samples collected after the ventriculostomy and ICP measurement compared to those collected before the procedure (Figure 4). The levels of tested proinflammatory cytokines in the CSF showed no association to the presence of Ab or HPt in brain Biomarkers of neuronal damage There was no significant relation of CSF NFL or MBP levels to the brain biopsy findings or to the diagnosis of iNPH or AD. In iNPH, a tendency (p = 0.05) towards lower ventricular CSF NFL values was seen in shunt-responders (Table 2). This is the first study to explore Ab and sAPP isoforms, proinflammatory cytokines, and biomarkers of neuronal damage in the CSF in conjunction with cortical brain biopsy. The major finding in the current study was the demonstration of the independent role of sAPPa in iNPH, which is not explained by cortical Ab pathology. In iNPH, decreased levels of sAPP isoforms in lumbar CSF have been reported in previous studies [2628]. As predicted, the level of lumbar CSF sAPPa was lower, whereas sAPPb showed a similar trend in patients with shunt-responsive iNPH compared to noniNPH patients in our patient cohort. Interestingly, in ventricular samples no association with iNPH was noted, which could be explained by the ventriculostomy procedure as an invasive sample collection method or by concentration of proteins in lumbar CSF. However, the reason for unaffected ventricular levels of sAPP isoforms are obscure as the ventricular CSF could be expected to reflect the periventricular metabolism better than lumbar CSF. The pathobiological role of sAPP isoforms in iNPH seems to be unconnected to the amyloidogenic pathway as there was no correlation between sAPP isoform levels in the CSF and Ab load in the cortical brain biopsy. The reason for the lowering of sAPP isoform levels in untreated iNPH remains unclear. However, as the levels are restored upon successful shunt treatment it has been hypothesized that the lowering may reflect metabolic impairment in brain tissue affected by iNPH [28]. In any case, our data suggest that the observed sAPP isoform level changes are independent of Ab pathology or are so early in the cascade that they do not reflect current tissue pathology. As expected, CSF levels of Ab42 showed a negative association and correlation to Ab load in the brain, as published earlier [17], and as suggested by amyloid-imaging studies [44]. In contrast, no such association was seen between the CSF levels of Ab38 or Ab40 with positive Ab or HPt immunoreactivity in the cortical brain biopsy. Our findings support the non-amyloidogenic role of Ab38 and Ab40 in the living human brain. As predicted, AD patients had lower Ab42 and higher p-tau levels in the CSF compared to non-AD patients, although the differences did not reach statistical significance. One explanation to this is that non-AD patients show similar CSF Ab42 and p-tau findings as biomarkers of comorbid AD tissue pathology without clinical dementia of Alzheimers type. As no pathological hallmark lesions have been identified in iNPH [3], the role of Ab and tau in iNPH remain elusive. However, there are patients with mixed pathologies i.e., patients with AD-related pathology and later dementia but still initial objective response for shunt surgery [10]. Interestingly, patients with iNPH + AD had lowest levels of CSF Ab42, and highest frequency of APOE-e4 carriers. Eighty percent (8/10) of these patients showed a favourable response to shunt treatment. In contrast to two previous studies [22,23], we found no prognostic potential in the levels of CSF Ab42 or tau in shunted iNPH patients. The differences in the results may be explained by the different sample collection time (most the samples in the current study were collected after ventriculostomy). It should also be noted that cited studies included fewer patients. In iNPH, reports of abnormal levels of proinflammatory cytokines (IL-1b, IL-4, IL-10, MCP-1, TNF-a) in the CSF has been published [28,31,32,34], while contradictory findings in studies comparing proinflammatory cytokines (IL-8, IL-10, IL-12 (p40 and p70), IFN-c, TNF-a, TGF- b1) have also been reported [28,33,35]. In the current study,we made an attempt to measure a wide panel of different proinflammatory cytokines from ventricular and lumbar CSF, and positive correlations between cytokines were seen. However, we also noted that most of the cytokines are present in CSF at concentrations that are close to or below the lower limit of detection of the assay. In fact, these low concentrations, which are technically challenging to measure, may explain some of the varying results in the published literature. Here, we focused on the cytokines that could be robustly quantified in at least a subset of samples, i.e., IL-8. No association of proinflammatory cytokines in the CSF with the diagnosis of iNPH or AD or the presence of Ab or HPt in brain biopsy was seen. In patients with iNPH, proinflammatory cytokines did not show prognostic value in shunt surgery. Consequently, our data suggest the role of neuroinflammation in iNPH and AD to be of little importance. Instead, an inflammatory response was seen in lumbar CSF samples collected after the ventriculostomy and ICP measurement. In addition, increased CSF tau and p-tau levels were observed in lumbar samples obtained after ventriculostomy as reported in earlier studies [17,39]. In consequence, levels of biomarkers in post-ventriculostomy lumbar CSF may not reflect the true values of the biomarkers in these patients. Previous studies have reported increased levels of CSF NFL in NPH [28,3639]. In our cohort, NFL showed higher lumbar CSF levels in patients with shunt-responsive iNPH compared to noniNPH patients (Table 2), but not to a significant degree. Interestingly, iNPH patients with positive shunt response showed a tendency towards lower NFL levels in ventricular CSF compared to shunt-nonresponsive iNPH patients. As NFL reflects subcortical axonal damage, perhaps high NFL could represent more severe and less recovering injury in the hydrocephalic brain. The strengths of the current study include: a large NPH cohort evaluated by cortical brain biopsy, utilization of ventricular and lumbar CSF samples in the analyses, a wide panel of tested CSF biomarkers, and evaluation of clinical outcome and other dementing disorders in the follow-up. The limitations of this study included: a limited number of patients with no shunt response, systematic assessment of shunt response only at 23 months, dichotomised shunt response scale, lack of validated objective outcome measures, and lumbar CSF sample available only in half of the cases. It is obvious that at least in prospective research setting shunted patients should be followed-up for a significantly longer time, and validated outcome measures should be utilized. Five patients with diagnostic findings suggesting iNPH did not respond to shunt possibly due to comorbidities or misdiagnosis, and thus these patients were excluded from the comparisons of (true) iNPH patients and non-iNPH patients in addition to the iNPH patients who were diagnosed with comorbid AD in the follow-up. More basic science and clinical studies evaluating the biology and potential role as diagnostic and prognostic biomarker of sAPPa and b are needed in the future. To conclude, the role of sAPP isoforms in iNPH seems to be unconnected to the Ab cascade pathway, but rather may be explained by a metabolic failure or ischemia in the brain. No elevations in the levels of proinflammatory cytokines in the CSF were observed in the different diagnostic groups. Consequently, neuroinflammation in iNPH and AD require further study. None of the tested CSF biomarkes showed a potency to discriminate between iNPH and non-iNPH patients or shunt-responders and nonresponders in iNPH in clinical setting. Supporting Information Acknowledgments The authors wish to acknowledge Marita Voutilanen, RN, for maintenance of the Kuopio NPH Registry. Author Contributions Conceived and designed the experiments: IA HS JEJ MH VL. Performed the experiments: OTP ML JR ON TTS S-KH AMK IA LP SS HZ VL. Analyzed the data: OTP ML JR ON TTS S-KH AMK IA LP MH VL. Contributed reagents/materials/analysis tools: IA HS JEJ MH HZ VL. Wrote the paper: OTP VL. Critical revision of the manuscript for important intellectual content: OTP ML JR ON TTS S-KH AMK IA LP SS HS JEJ MH HZ VL. Statistical analysis: OTP ML VL. Obtained funding: HS MH HZ VL. Study supervision: HS MH VL. 1. 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Okko T. Pyykkö, Miikka Lumela, Jaana Rummukainen, Ossi Nerg, Toni T. Seppälä, Sanna-Kaisa Herukka, Anne M. Koivisto, Irina Alafuzoff, Lakshman Puli, Sakari Savolainen, Hilkka Soininen, Juha E. Jääskeläinen, Mikko Hiltunen, Henrik Zetterberg, Ville Leinonen. Cerebrospinal Fluid Biomarker and Brain Biopsy Findings in Idiopathic Normal Pressure Hydrocephalus, PLOS ONE, 2014, DOI: 10.1371/journal.pone.0091974