David Calligaris wins ABC Best Paper Award

Analytical and Bioanalytical Chemistry, May 2016

Nicola Oberbeckmann-Winter

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David Calligaris wins ABC Best Paper Award

Anal Bioanal Chem David Calligaris wins ABC Best Paper Award Nicola Oberbeckmann-Winter 0 1 2 3 0 Who is David Calligaris? 1 Analytical and Bioanalytical Chemistry , Springer, Tiergartenstrasse 17, 69121 Heidelberg , Germany 2 Nicola Oberbeckmann-Winter 3 David Calligaris talks about his research , his motivations, and about himself to Nicola Oberbeckmann-Winter The ABC Best Paper Award 2015 for outstanding work published in Analytical and Bioanalytical Chemistry (ABC) goes to David Calligaris (35), who is author of the paper BProfiling of adrenocorticotropic hormone and arginine vasopressin in human pituitary gland and tumor thin tissue sections using droplet-based liquid-microjunction surface-samplingHPLC-ESI-MS-MS,^ which describes profiling analyses of pituitary hormones using a novel approach that allows spatially resolved sampling, HPLC separation, and mass spectrometric detection. This study demonstrates the usefulness of liquid chromatography coupled to mass spectrometry for the analysis of complex samples, such as surgical samples, within timelines that fit with near-real-time diagnosis. Accompanied by 1500 euros, the award is sponsored by Springer to honor exceptional young scientists and to stimulate their research careers. The article BProfiling of adrenocorticotropic hormone and arginine vasopressin in human pituitary gland and tumor thin tissue sections using droplet-based liquid-microjunction surface-sampling-HPLC-ESI-MS-MS^ by Vilmos Kertesz, David Calligaris, Daniel R. Feldman, Armen Changelian, Edward R. Laws, Sandro Santagata, Nathalie Y. R. Agar, and Gary J. Van Berkel is available online at http://link.springer.com/article/10.1007%2Fs00216-0158803-2 or can be requested as a pdf. - David Calligaris was born in 1980. He earned a B.S. degree in Biochemistry from the Victor Segalen Bordeaux 2 University (Bordeaux, France) in 2005, and an M.S. degree in the Structure and Function of Macromolecules and Life Processes from the Paul Sabatier Toulouse III University (Toulouse, France) in 2007. He received his Ph.D. in Human Pathology with a specialization in Oncology from the Aix-Marseille University (Marseille, France) in 2011 under the supervision of Daniel Lafitte. He is currently an Instructor in the Department of Neurosurgery at Brigham and Women’s Hospital—Harvard Medical School, under the direction of Professor Nathalie Y. R. Agar. He is developing mass spectrometry based approaches for the near-real-time intraoperative diagnosis of tumors. He is also performing drug development research using mass spectrometry based pharmacokinetics/pharmacodynamics approaches, and setting up a platform coupling microwell array and single-cell imaging for the identification of clinical markers of drug treatment response. What was your motivation to do research on mass spectrometry applied to near-real-time intraoperative diagnosis? Being able to characterize biological tissues with high sensitivity and specificity using mass spectrometry and provide more adequate tools to surgeons to delineate tumors and ultimately result in more precise surgery for patients motivated me to pursue my research in the Surgical Molecular Imaging Laboratory and continue to develop this technique to improve patient healthcare. Why do you think your work merits the ABC Best Paper Award? This work merits an award because it demonstrates that the droplet-based liquid-microjunction surface-samplingHPLC–ESI-MS–MS system developed at Oak Ridge National Laboratory has the capability to differentiate normal from tumor tissue in a shorter timeframe than traditional histopathology evaluation, and is based on the rapid analysis of peptides and proteins. So far, real-time or nearreal-time mass spectrometry work that could support surgical decisions has been based on the analysis of metabolites (<1500 Da). In addition, the use of liquid chromatography coupled to mass spectrometry for the multidimensional separation of complex samples (such as surgical samples) allows the rapid and simultaneous detection of multiple and established tumor markers such as proteins. How does the Award-winning work relate to your research? One of the main projects I work on is to implement mass spectrometry into the Advanced Multimodality Image Guided Operating (AMIGO) suite at Brigham and Women’s Hospital to perform real-time brain tumor diagnosis from surgical samples. The results of the award-winning article are closely related to my research b e c a u s e t h e y p r e s e n t a d r o p l e t - b a s e d l i q u i d microjunction surface-sampling-HPLC–ESI-MS–MS system that could be used for near-real-time tumor diagnosis during surgery. How would you explain your research to children? We test if instruments named mass spectrometers could help surgeons to detect tumors during surgery. What’s the trickiest problem you’ve had to overcome in your research? How did you solve it? The trickiest problem arises each time you acquire data from biological samples, namely, making sense of the data and bringing it into perspective. Being highly critical about these data is the only way to solve the problem. Where do you see your field headed and how do you see it influencing bioanalytical research? Mass spectrometry is becoming an essential tool in the field of clinical and biomedical research applications. The development of a robust workflow, including guidelines for the training of medical staff, protocols for sample preparation, and a powerful data analysis platform, will effectively bring mass spectrometry from the bench to the bedside. Which incident/discovery has proved most valuable for your own research? Which recent discovery might prove most valuable to the field of (bio)analytical research or beyond? I would have to say the introduction of all methods used for sample analysis into an operating theater. This includes mass spectrometry used for the analysis of airway gases from anesthetized patients in the 1980s (Ozanne et al. Anesthesiology 1981;55:62–70). The development of multiplexed ion beam imaging—a valuable method that allows the analysis of samples stained with metal-isotope-labeled antibodies for the simultaneous detection of and differentiation between multiple antigens in biological samples. What was the best advice you ever received? Always have a Plan B in case Plan A does not go well. Whom do you admire the most or who inspired you the most and why? I would have to say that all of the advisors I have had, Dr. Sandrine Uttenweiler, Dr. Daniel Lafitte, Dr. Pascal VerdierPinard, Professor Edwin de Pauw, and Professor Nathalie Agar, inspired me. They all supported me and gave me good advice to make the right decisions for the research projects I was/have been in charge of. What are your future plans? To continue contributing to mass spectrometry based cancer research efforts. What do you do in your spare time?


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Nicola Oberbeckmann-Winter. David Calligaris wins ABC Best Paper Award, Analytical and Bioanalytical Chemistry, 2016, 4823-4824, DOI: 10.1007/s00216-016-9530-z