Mechanistic Insights into the Efficacy of Sodium Bicarbonate Supplementation to Improve Athletic Performance
Siegler et al. Sports Medicine - Open
Mechanistic Insights into the Efficacy of Sodium Bicarbonate Supplementation to Improve Athletic Performance
Jason C. Siegler 0
Paul W. M. Marshall 0
David Bishop 1
Greg Shaw 3
Simon Green 0 2
0 School of Science and Health, Sport and Exercise Science, Western Sydney University , Locked Bag 1792, Penrith, NSW , Australia
1 Institute of Sport, Exercise and Active Living (ISEAL), Victoria University , Melbourne , Australia
2 School of Medicine, Western Sydney University , Sydney , Australia
3 Australian Institute of Sport , Canberra , Australia
A large proportion of empirical research and reviews investigating the ergogenic potential of sodium bicarbonate (NaHCO3) supplementation have focused predominately on performance outcomes and only speculate about underlying mechanisms responsible for any benefit. The aim of this review was to critically evaluate the influence of NaHCO3 supplementation on mechanisms associated with skeletal muscle fatigue as it translates directly to exercise performance. Mechanistic links between skeletal muscle fatigue, proton accumulation (or metabolic acidosis) and NaHCO3 supplementation have been identified to provide a more targeted, evidence-based approach to direct future research, as well as provide practitioners with a contemporary perspective on the potential applications and limitations of this supplement. The mechanisms identified have been broadly categorised under the sections 'Whole-body Metabolism', 'Muscle Physiology' and 'Motor Pathways', and when possible, the performance outcomes of these studies contextualized within an integrative framework of whole-body exercise where other factors such as task demand (e.g. large vs. small muscle groups), cardio-pulmonary and neural control mechanisms may outweigh any localised influence of NaHCO3. Finally, the 'Performance Applications' section provides further interpretation for the practitioner founded on the mechanistic evidence provided in this review and other relevant, applied NaHCO3 performance-related studies.
For over 40 years, researchers have explored the efficacy
of inducing alkalosis to enhance athletic performance.
Although many buffers have been studied (e.g. sodium
citrate, sodium phosphate, sodium lactate), evidence
supports sodium bicarbonate (NaHCO3) as the most
consistently effective agent for improving exercise
performance. Meta-analyses have reported that
supplementation can result in an approximately 2 to 3 %
improvement in a variety of performance measurements
(e.g. power, speed, work capacity, time to failure) during
both single and repeated bouts of high-intensity exercise
[1, 2]. Oral administration of NaHCO3 generally
increases blood buffering capacity (Fig. 1)  and is
believed to attenuate the increase in intramuscular acidity
© 2016 The Author(s). Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0
International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and
reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to
the Creative Commons license, and indicate if changes were made.
Fig. 1 Resting changes in blood bicarbonate [HCO3−] and pH after ingesting 0.2 and 0.3 g·kg−1 of sodium bicarbonate (NaHCO3) (reproduced
from Siegler et al.  with permission)
synonymous with high-intensity exercise and skeletal
muscle fatigue [1, 4], although the physiologic
mechanisms directly responsible for performance
augmentation in humans are unclear. Indeed, a large proportion
of empirical research and reviews investigating the
ergogenic potential of NaHCO3 focus predominately on
performance outcomes (e.g. time to task failure, cumulative
work accomplished, time trial performance) [1, 4, 5],
and only speculate about underlying mechanisms
responsible for attenuating skeletal muscle fatigue.
Mechanistic links between NaHCO3 supplementation,
skeletal muscle fatigue and athletic performance are
often underpinned by the belief that metabolic acidosis,
or a decline in muscle pH, contributes to skeletal muscle
fatigue through inhibition of various metabolic processes
and/or rates of contractile cycling [6–9]. However, it has
also been argued that acidosis has little influence on
maximal force production , and in some instances
may even facilitate maximal force and velocity of
shortening . The debate as to whether or not metabolic
acidosis, or indeed the intra- and extracellular
distribution of other relevant ions (e.g. Ca2+, K+, Cl−), has an
inhibiting or facilitating effect on contractile function
continues today and has been summarised in recent
reviews [12–14]. The primary aim of this review was to
fundamentally examine the influence of NaHCO3
supplementation on metabolic acidosis and mechanisms
associated with skeletal muscle fatigue and athletic
performance. Secondly, the mechanistic evidence
presented in this review has been contextualised in the
‘Performance Applications’ section to provide
contemporary, evidence-based recommendations for
practitioners and athletes.
After the ‘Historical Overview’ section, studies have been
broadly categorised under sub-headings, and when
possible, the performance outcomes of these studies
contextualized within an integrative framework of whole-body
exercise where other factors such as task demand (e.g.
large vs. small muscle groups), cardio-pulmonary and
neural control mechanisms may outweigh any localised
influence of NaHCO3. In many of these studies,
NaHCO3 is also used in conjunction with an acid (e.g.
ammonium chloride (NH4Cl)) to determine the wider
contribution of acid-base perturbation on skeletal
muscle fatigue; however, emphasis has been placed on
reviewing only the alkalosis condition in most
instances. This review does not address all
performancebased applications of NaHCO3 supplementation in the
literature, such as team sport performance [15–17],
skill sports [18–20], hydration [21, 22] or the efficacy
of NaHCO3 under hypoxic conditions [23–25].
Associations between NaHCO3, skeletal muscle fatigue
and exercise performance can be traced back to what is
believed to be the first paper published on the topic in
1931 from the Harvard Fatigue Laboratory . This
study was one of many in this era more broadly focused
on respiratory and metabolic buffering mechanisms
associated with what was termed ‘lactic acidosis’ .
Indeed, there was considerable interest in lactic acidosis
and fatigue during muscle contractions and exercise as
early as the 1900s and continuing through to the 1970s
. However, beyond a few subsequent investigations
[28–31], research on the ergogenic potential of NaHCO3
did not become prominent until the mid-1970s.
The first series of exercise performance studies to be
formally presented from one laboratory were from Jones
and colleagues starting in 1977 [32–35]. Collectively,
these were also the first studies on humans to
incorporate both an alkali (NaHCO3) and an acid (NH4Cl) to
explore the impact of altered acid-base status on markers
of cardiovascular, respiratory and metabolic function in
conjunction with exercise performance. In the first two
studies, cycling at 95 % of VO2max to volitional
exhaustion after NaHCO3 ingestion elicited between a 30 and
40 % performance improvement, respectively [32, 33],
whereas a following study requiring a 30-s maximal
effort at 100 rpm only resulted in a small but
nonsignificant improvement in maximal power . In the
fourth study, incorporating an incremental cycling
protocol (~16 W min−1 at 60 rpm to volitional
exhaustion), only the NH4Cl condition induced a performance
decrement whereas there was no difference between the
NaHCO3 and control conditions . The authors
accounted for the performance discrepancies by relating
exercise task and duration to a decline in muscle pH
and the concurrent effect on the rate of glycolysis.
Furthermore, they suggested that the beneficial effects of
enhanced H+ removal would be more advantageous
during the prolonged cycling tasks (e.g. >4 min) than during
the shorter, 30-s efforts . The discrepancy in
performance outcomes in these studies is also the first of
many examples where different exercise task demands
and NaHCO3 dosing protocols may have impacted upon
From the mid-1980s, performance-related NaHCO3
studies began to emerge prominently in the literature.
The increased interest may have been influenced by
earlier findings [32, 33], but was probably also related to the
ease of oral NaHCO3 administration and the short time
course required to acutely induce metabolic alkalosis
that provided the opportunity to conduct a wide variety
of performance-related studies within a relatively short
period of time [36–40]. Collectively, the studies during
this period that reported an ergogenic benefit of
NaHCO3 ingestion generally adhered to a maximal or
supramaximal (e.g. 125 % VO2max) exercise task lasting
between 1 and 7 min (Table 1) [41, 42]. Many of the
performance-based studies in this era also proposed a
similar study rationale; one based on previous equivocal
performance outcomes and/or on the attenuation of the
decline in muscle pH proposed to occur during high
rates of glycolytic flux after NaHCO3 ingestion [39, 40,
43–45]. Indeed, these rationales persist in many
published papers today [46–49], further illustrating that
beyond the effect on glycolysis, the other potential
physiologic mechanisms responsible for performance
augmentation after NaHCO3 supplementation have
received limited attention in the applied research domain.
From an energetic perspective, any physiological effects
of NaHCO3 supplementation that translate to an
increase in performance should be observed in the rates
and amounts of energy provision and/or the efficiency of
energy transduction to power and work. However, very
little research has investigated, or indeed attempted to
quantify, anaerobic and aerobic energy yields,
accumulated oxygen deficits or energetic efficiency during
conditions of metabolic alkalosis. Beyond a handful of
studies that have modelled whole-body VO2 kinetics
(e.g. VO2 fast and slow components [50–52]), most of
the research on NaHCO3 and metabolism has been
conducted on single muscle or isolated muscle groups and
their metabolic response to various task demands.
From studies conducted in the 1970s [32, 53–55], a
causal link between increased intramuscular lactate (La−)
concentrations and La− efflux, reduced proton (H+)
accumulation and improved muscular performance had been
posited to explain the performance improvements
observed following NaHCO3 supplementation . To further
clarify the influence of NaHCO3 on intramuscular [La−]
and La− efflux during intense exercise, Spriet and
colleagues minimised the limitations associated with in vivo
contracting muscle measurement (e.g. varying work rates
and metabolite accumulation) by applying continuous
supramaximal stimulation (5 V at 0.5 Hz) for 20 min to a
perfused gastrocnemius-plantaris-soleus (GPS) muscle
group . NaHCO3 increased the rate of La− efflux from
the muscle group, but did not attenuate the decline in
muscle tension as compared to control conditions .
Methodological limitations inherent to the perfusion and
Table 1 A summary of studies conducted in the mid 1980’s that reported an ergogenic effect of 0.3 g kg−1 sodium bicarbonate
(NaHCO3) supplementation on performance tasks lasting approximately −7 min
Costill et al. ‘84 
Gao et al. ‘86 
McKenzie et al. ‘86  VO2max: 3.83 ± 0.61 L min−1
University track athletes
Well-trained college swimmers 5, 100-yd freestyle swim
(VO2max: 4.3 ± 0.1 L min−1)
5, 1-min cycling bouts (125 % VO2max; 113.5 ± 12.4 s
5th bout to exhaustion)
Goldfinch et al. ‘88  Male athletes
Subject characteristics are represented to the level of detail provided in the original published studies. All values in the (%) improvement column were
significantly different from control (p < 0.05)
stimulation protocol only allowed the authors to
speculate as to whether or not the increased La− efflux was a
result of increased rates of glycogenolysis and/or
glycolysis, or due to an increased rate of La− release from the
Nearly 15 years later, Hollidge-Horvat and colleagues
used human muscle tissue samples obtained at different
steady-state exercise intensities (i.e. 30, 60 and 75 %
VO2max) to investigate the mechanisms responsible for
the increased La− efflux by then commonly observed
after NaHCO3 ingestion . These authors eloquently
detailed a complex, intensity-dependent response in key
metabolic enzymes and regulatory steps after NaHCO3
ingestion as compared to control conditions. Briefly, the
authors observed a similar increase in La− efflux to that
of previous studies after NaHCO3 ingestion, but only at
75 % VO2max . The authors speculated that the
increased [La−] observed in circulation may have been due
to a decrease in La− uptake by inactive tissue  or an
altered H+ gradient on the transporters (later confirmed
by others as an increase in monocarboxylate transporter
(MCT) activity ). The authors also documented an
increased degradation of muscle glycogen in the
NaHCO3 compared to the control trials . As
performance was not assessed in this study, whether or not
this increased reliance on muscle glycogen stores in the
NaHCO3 trial would have influenced performance at the
higher intensities remains unknown.
As the capacity to study exercising muscle
metabolism in vivo improved beyond the limitations of the
biopsy, others have subsequently expanded upon these
findings to include exercise performance measures.
Using phosphorus-31 magnetic resonance spectroscopy
(31P-MRS) and a constant-rate progressive wrist-flexion
exercise (1-s contraction/1-s relaxation) to volitional
exhaustion, Raymer and colleagues reported an
approximate 12 % increase in time to failure after
NaHCO3 supplementation and attributed the
performance effect to a delayed onset of intracellular acidosis
(evident at ~60 % peak power) in the alkalotic
condition . Furthermore, concomitant to the delayed
intracellular acidosis was a delay in the slow-to-rapid
increase in the [PCr]/[Pi] to power output relationship
(repeatedly observed in subsequent studies by this same
group [61, 62]). As metabolic perturbation caused by
acidosis had been associated with the inhibition of
glycolysis and glycogenolysis , increased
phosphocreatine (PCr) degradation and inorganic phosphate (Pi)
accumulation ([PCr]/[Pi]) within the myocyte , the
authors speculated that the delayed accumulation of
intracellular H+ induced by NaHCO3 was the primary
cause of the performance effect.
To briefly summarise, much of the research in this
area has been centred around muscle metabolism, from
the earlier observations on intramuscular [La−] and La−
efflux to the more recent focus on glycolytic
intermediates and high-energy phosphate kinetics (e.g. PCr).
NaHCO3 appears to effect the [PCr]/[Pi]-power
relationship, glycolytic intermediates and the intra- and
extracellular distribution of metabolites and other strong ions
(Fig. 2) [56, 57, 60, 65, 66]. Not surprisingly, this effect is
more pronounced during periods of prolonged, intense
contractile activity where a larger proportion of energy
provision is derived from classically defined anaerobic
pathways. Contextually, this effect may also partially
explain the ergogenic findings of many performance-based
studies on athletes that have imposed a high demand on
these pathways either with single or repeated bouts of
intense exercise [39, 67–70].
Inducing a state of alkalosis also affects the intra- and
extracellular balance of other strong ions such as Na+, K+
and Cl− , all of which contribute to the maintenance
of skeletal muscle contractile function . In general,
and irrespective of acid-base balance, fatiguing exercise
induces ion concentration changes between the intracellular,
interstitial and extracellular compartments. Although
ultimately dependent upon the level of contractile activity,
as well as diffusion and perfusion limitations, muscle K+
will tend to decrease whereas muscle Na+, Cl− and Ca2+
will tend to increase with intense exercise (H+ will
increase in both intra- and extracellular compartments)
. Collectively, changes in these ionic concentrations
have been demonstrated to decrease resting membrane
potential and impair sarcolemmal excitability . The
effect of NaHCO3 specifically on the distribution of these
ions between compartments, in particular the
NaHCO3−induced reduction in K+ efflux from the muscle, has been
the focus of other studies investigating the effect of
alkalosis on the interstitial and plasma concentrations of strong
ions and skeletal muscle fatigue [65, 66, 71].
In terms of exercise performance, however, only
Sostaric and colleagues have demonstrated an improvement
after NaHCO3 supplementation . By incorporating a
small muscle mass, and therefore inducing a larger
reduction in muscle K+ than commonly observed during
whole-body exercise , the authors hypothesised that
NaHCO3 would attenuate the augmented K+ efflux from
the small muscle mass and therefore better preserve
muscle function . Subsequently, they observed an
increase in total exercise time of greater than 2 min
(~25 % improvement) compared to a placebo during a
constant rate, progressive concentric forearm flexion
exercise task to volitional exhaustion . NaHCO3 also
improved the regulation of circulating K+ and handling
of Cl− (but not Na+), which the authors proposed to
Fig. 2 Overview of the mechanisms associated with sodium bicarbonate supplementation (NaHCO3) and whole-body metabolism, muscle physiology
and motor pathways. Definitions for the following abbreviations are provided for mH+ (muscle protons), mLa− (muscle lactate), MCT (monocarboxylate
transporters), bLa− (blood lactate) and [PCr]/[Pi] (ratio of phosphocreatine to inorganic phosphate)
have better preserved membrane excitability and
therefore contributed to the performance improvement .
NaHCO3 appears to delay the rise in muscle interstitial
K+ that occurs during intense contractile activity and,
via this effect, may attenuate the decline in muscle
membrane excitability and the extent of K+-induced
inactivation of myocytes during such activity [11, 66]. As
previously stated, this effect may have contributed to not
only the performance improvement observed by Sostaric
and colleagues but also the earlier findings of Raymer as
both used a similar exercise performance task [60, 65].
However, these studies also illustrate the relevance of
muscle mass and task demand when determining the
efficacy of NaHCO3 as an ergogenic supplement. Issues
such as agonist/antagonist muscle actions, synergistic
contraction of larger muscle groups and recruitment
order (e.g. Hennemen’s size principle)  during
whole-body exercise may mitigate the performance
effects observed in smaller, isolated muscles. As an
example, the trend of NaHCO3 improving incremental
exercise to volitional exhaustion in smaller muscle
groups [60, 65] is less consistent when large muscle
groups (e.g. cycling) or whole-body exercise is
incorporated within a similar task [32, 33, 35, 74, 75] (Table 2).
Influence of Fibre-Type
Although earlier work had illustrated a relationship
between muscle pH and skeletal muscle force and/or muscle
shortening velocity [76–78], the first to show an
independent effect of acidosis on fast- and slow-twitch fibres
appears to be Metzger and Moss in 1987 . Using single
fibres of rat soleus (SOL) and vastus lateralis (VL)
muscles, previously characterised as predominately type I
(SOL) or IIa and b (VL), respectively [80, 81], maximal
isometric tension and maximum shortening velocity was
assessed as a function of pH. Although initial declines in
pH induced a concomitant reduction in tension and
shortening velocity in both SOL and VL, as pH decreased
below 6.2, the relative loss in tension was greater in VL
. The first to study the effect of NaHCO3 on
fibrespecific force production was Lindinger and colleagues in
1990 . These authors examined the effects of NaHCO3
on SOL and white gastrocnemius (WG) muscle tension at
Table 2 A summary of the results of studies investigating the influence of 0.3 g kg−1 sodium bicarbonate (NaHCO3)
supplementation on whole-body and isolated muscle incremental exercise to volitional exhaustion
Sostaric et al. ‘05 
Isolated (concentric finger flexion)
Poulus et al. ‘74 
Jones et al. ‘77 
Sutton et al. ‘81 
Kowalchuk et al. ‘84 
Housh et al. ‘91 
Increased 16 W min−1 at 60 rpm
Continuous (2-min stages) physical work capacity test
(PWCFT) starting at 60 W to volitional exhaustion
~12 % improvement
All values represented as having an improvement (%) were significantly different from control or placebo (p < 0.05)
rest and during 5 min of intense stimulation .
Although NaHCO3 differentially altered the ionic
composition (specifically K+, Cl− and Mg2+) and the rate of
La− efflux from the muscle fibres, it had no effect on
muscle tension in either muscle .
In 2007, Broch-Lips and colleagues stimulated the
SOL and extensor digitorum longus (EDL), another
muscle characterised as predominately type IIa, under
normal or alkalotic conditions. In addition to the
isometric force measurement, the authors also estimated
rate of force development (RFD) as the maximal
numerical slope value of the force development during
contraction . Although NaHCO3 induced increases in
pH from 7.4 to 7.6, neither maximum force nor RFD
was affected in either muscle . However, more recent
evidence using a fatiguing, mechanically controlled
stretch-shortening work cycle model has shown that
NaHCO3 administration may improve RFD .
Avoiding the limited degree of shortening that occurs during
isometric contractions , Higgins and colleagues
subjected both SOL and EDL mouse muscles to repeated
length changes matched to the expected stride frequency
of the mice during normal locomotion (10 % strain with
a cycle frequency for EDL of 8 Hz and SOL of 5 Hz) and
demonstrated an approximate 7 % improvement in force
production during shortening in the EDL compared to a
3 % improvement in the SOL after NaHCO3 incubation.
To date, the fibre-type dependent findings related to
contractile properties have not been replicated in the
exercising human given the methodological constraints
addressed earlier of isolating fibre-type contributions to
whole-body locomotion , quantifying properties of
diffusion and other factors associated with
intramuscular blood supply [85, 86]. However, collectively, this
body of research suggests that NaHCO3 may affect the
metabolic properties associated with contractile
shortening velocity and that this effect appears more
pronounced in fast-twitch fibres (Fig. 2). The effect on
rates of contractile shortening but not maximal force
may also explain the limited efficacy of NaHCO3
observed in performance-based studies only measuring
maximal voluntary force [87, 88].
Interestingly, although shortening velocity of slow-twitch
fibres after acute NaHCO3 administration does not
appear to be improved when compared to fast-twitch
fibres, chronic ingestion coupled with high-intensity
training may improve the oxidative capacity of these
fibres [89–91]. After 8 weeks (three times per week) of
chronic NaHCO3 ingestion coupled with high-intensity
(between 140 and 170 % lactate threshold (LT))
intermittent training, Edge and colleagues reported a significant
improvement in both LT (~11 %) and performance
(~25 %) (time to failure) in moderately trained females
when compared to a control group matched for total
work (kJ). The authors speculated that as H+
accumulation has been shown to impair oxidative capacity and
various components of cellular respiration , training
in an alkalotic state may have attenuated the impairment
and allowed for prolonged cellular respiration to occur
during training .
Continuing this line of investigation, two subsequent
studies by the same group used relatively similar
NaHCO3 dosing and high-intensity training protocols
on rats to explore fibre-type dependent protein
expression associated with H+ removal during rest and
exercise [90, 91]. In the first study, the authors
observed a differential effect of alkalosis on MCT-4
expression in the SOL but not EDL when compared to
placebo and control conditions . Additionally,
citrate synthase concentration was higher in the alkalotic
condition (23 vs. 16 %) after training in the SOL but
not EDL. These findings led the authors to conclude
that chronic NaHCO3 ingestion was influencing
protein expression associated with slow but not
fasttwitch fibres. The final study in the series appeared to
further confirm this observation, with the authors
reporting an increase in mitochondrial respiration in
the SOL as compared to the EDL after training in an
induced alkalotic state .
The evidence that chronic use of NaHCO3 coupled
with appropriate training may lead to aerobic
adaptations associated with improved mitochondrial efficiency
in slow-twitch fibres is intriguing , and future work
may clarify whether this is indeed a viable option for
improving aerobic efficiency. Additionally, we are unaware
of any studies that have investigated the efficacy of
introducing chronic NaHCO3 supplementation on
mechanisms associated with muscle force production or rapid
force-generating capacity. Given the preliminary
evidence that NaHCO3 may exhibit independent effects on
fibre-specific mechanisms associated with improved
contractile function both acutely and chronically, future
study in this area is warranted.
Ultimately, determining the mechanisms by which
NaHCO3 supplementation can improve athletic
performance may require a more integrative understanding
of other systems inherently involved during exercise, and
more specifically the progression of skeletal muscle
fatigue. The motor pathways, or more explicitly the
neuromuscular system, are intimately involved in maintaining
skeletal muscle function during fatiguing exercise. This
complex integrative network is often overlooked in the
NaHCO3 literature as a possible contributor to
wholebody exercise performance. Although partially due to
the limited number of studies observing the influence of
NaHCO3 on the motor pathways, studies in this section
have been broadly categorised as they relate to motor
unit recruitment and feedback mechanisms associated
with metabolite accumulation in the periphery that have
been directly linked to central drive.
As addressed in the ‘Whole-body Metabolism’ section,
fluctuations in strong ions (e.g. K+, Na+, Cl−) have also
been linked with maintaining resting membrane
potential and sarcolemmal excitability . However, few
studies have explored whether inducing pre-exercise
alkalosis via NaHCO3 ingestion influences these
mechanisms as they relate to excitation-contraction coupling
and ultimately muscle force generating capacity [92–95].
Hunter and colleagues investigated the effects of
NaHCO3 ingestion on muscle fibre conduction velocity
after fatiguing exercise (i.e. 50 min of cycling at 105 %
lactate threshold) . Using a previously developed
technique , these authors observed an attenuation in
the decline in muscle fibre conduction velocity after
cycling in an alkalotic state . This improvement,
however, did not translate into an improvement in maximal
knee extension force.
We have also demonstrated that NaHCO3 has no
effect on maximal force production . However, we have
observed an improvement in the ability to rapidly
generate force (RFD), a functional measure related to both
rapid motor unit recruitment and contractile shortening
velocity . In this study, subjects performed an
intermittent (30 s:30 s work-to-rest ratio) cycling protocol at
120 % of peak power until volitional exhaustion, where
maximal voluntary contractions (MVC) were performed
immediately after each 30-s work cycle. Although RFD
and maximal force declined in both NaHCO3 and
control trials, RFD was better maintained throughout the
exercise under alkalotic conditions . The
mechanisms responsible for the differential effect on RFD but
not maximal force after NaHCO3 ingestion requires
further investigation, as RFD is influenced by both
peripheral (e.g. excitation-contraction coupling) and central
(e.g. central drive) factors .
Mechanisms related to central drive such as cortical
output, spinal reflexes and muscle afferents have also
been shown to be influenced by the localised changes in
muscle metabolite concentrations (e.g. H+) associated
with exercise [99–101]. The complex interaction
between these mechanisms as they contribute to skeletal
muscle fatigue has been previously reviewed . Briefly,
they are often categorised as supraspinal (e.g. output of
descending motor cortical and corticospinal pathways to
the motoneurons) and sub-spinal (e.g. muscle spindles,
Golgi tendon organs, small diameter group III/IV
afferents) mechanisms  and their relative contribution to
skeletal muscle fatigue associated with the intensity of
the exercise task. The degree to which H+ accumulation
in the periphery affects central mechanisms, particularly
during intense exercise, has been related specifically to
the central projection of the mechanically and
chemically sensitive small diameter group III/IV afferents
located within the skeletal muscle [102, 103]. It is believed
that the exercise-induced actions of these afferent fibres
inhibit the excitability and net output of corticospinal
cells in the central nervous system, as well as effect
alpha motor neuron excitability [104–107].
Very few investigations have used NaHCO3, or indeed
other methods, to manipulate pH and to explore the
effects of alkalosis on afferent or other neural pathways
contributing to skeletal muscle fatigue [94, 95]. Based on
the assumption that H+ accumulation leads to a decline
in central drive to the muscle, Matsuura and colleagues
measured surface electromyography (sEMG) activity in
the vastus lateralis to determine whether motor unit
recruitment strategies during repeated high-intensity
cycling (i.e. repeated 10-s efforts at a resistive load (N) of
0.075 BM 9.81−1) would be influenced by NaHCO3
ingestion . Neither root mean square or mean power
frequency was influenced by pH, with both NaHCO3
and control conditions reflecting similar recruitment
profiles throughout the test . A follow-up study by
the same group suggested that NaHCO3 again had no
effect on gross sEMG activity or perceived sense of effort
after 2 min of cycling at 105–110 % of a pre-determined
maximal workload . However, sEMG signals lack
sensitivity to detect changes in the number and
discharge rate of active motor units that would indicate
increased central motor output [108, 109] and therefore
may not be sensitive enough to accurately determine
whether or not altering pH may affect descending
A more recent body of literature suggests a
relationship between increasing levels of intramuscular
metabolite concentrations analogous with high rates of skeletal
muscle contraction, and greater group III/IV afferent
firing in humans [110–112], which would ultimately act as
a protective mechanism against peripheral fatigue by
limiting descending central drive . Using an
isometric knee extension model in combination with ischemia
to enhance the firing frequency of group III/IV afferents
[114–116], we have observed voluntary activation, a
gross estimate of central drive, to be better preserved
under NaHCO3 conditions . The improved
activation during alkalosis, however, was not commensurate
with changes in net muscle excitation or rapid and
maximal force output which declined equally in both
NaHCO3 and control conditions . We speculated
that the divergence might have been a result of the
extreme conditions of peripheral fatigue induced by our
exercise protocol (2-min MVC of the quadriceps
followed by 2 min of ischemia and ending with a 1-min
ischemic MVC) . The findings may also reflect the
relatively minor role afferent feedback may play in
these situations of extreme peripheral fatigue, where
other factors separate to group III and IV afferents may
have contributed to the down-regulation of descending
The use of stimulation (e.g. nerve, muscle, transcranial
magnetic stimulation) to explore the potential link
between NaHCO3 and central and peripheral mechanisms
associated with skeletal muscle fatigue may provide
further insight toward the ergogenic potential of this
supplement. Moreover, our findings that alkalosis may affect
central mechanisms associated with early rate of torque
development warrants further investigation, as the ability
to rapidly recruit muscle may be influenced by the
contractile properties of the fibre (e.g. type I or II) (Fig. 2)
. As evidence suggests that acute NaHCO3 ingestion
may have a preferential effect on type II muscle fibres ,
further study comparing different combinations of
exercise task demands and muscle groups may also clarify the
effect of NaHCO3 on rates of torque development and
muscle recruitment in humans.
Competition and Training Recommendations
Appropriately identifying whether NaHCO3 ingestion
may influence the underlying mechanisms associated
with whole-body metabolism, muscle physiology and/or
motor pathways within the context of a particular
athletic endeavour or training stimulus is an important
initial step when considering the use of this supplement.
For example, in the context of athletic performance,
delayed onset of H+ accumulation may be relevant to
certain time frames within an event that demand rapid
transitions between steady-state and higher intensity
efforts (e.g. beginning a ‘long-finish’ earlier in a 1500-m
race). In this scenario, NaHCO3 supplementation may
be appropriate based on the evidence indicating an
attenuation of H+ accumulation during exercise intensities
transitioning between aerobic and anaerobic pathways
[60, 65, 89]. Another example, and specifically related
to the evidence surrounding RFD and fast-twitch fibres
[83, 97], would be to improve the propulsive force at
the start of an explosive movement (e.g. the initiation
of a pedal stroke). Considerations for the potential
benefit of NaHCO3 in these unique situations would
require extensive consultation within an athlete’s
support network, as well as a systematic integration of the
supplement to accurately determine any efficacious
response. Although often difficult to rigorously assess in
the elite environment, any marginal improvement in
performance gained by using NaHCO3 for these
specific purposes in competition may provide alternative
uses for this supplement that extend beyond current
recommendations [1, 5].
Although there have been few studies investigating the
efficacy of implementing a chronic NaHCO3
supplementation strategy on training outcomes [89, 119], given the
recent evidence in animal models exhibiting enhanced
oxidative/mitochondrial adaptations in slow-twitch
fibres after chronic supplementation [90, 91], further work
in this area may establish whether incorporating
NaHCO3 into an aerobic training paradigm is warranted.
Furthermore, with research also supporting a potential
ergogenic effect on RFD , particularly in fast-twitch
fibres , applying a loading strategy during specific
training and adaptation blocks (e.g. explosive power)
may also have merit. In practice, however, incorporating
a training strategy inclusive of a regular 0.3 g·kg−1
NaHCO3 load would require a systematic administration
and monitored approach. Although we are unaware of
any documented long-term adverse effects of chronic
loading in either sporting [89, 120, 121] or clinical
contexts [122–124], given the relatively high sodium content
(e.g. a 70-kg athlete would consume ~6 g), continually
monitoring both blood acid-base and strong ions (e.g.
Na+) would allow any irregularities from the ingestion
regimen to be detected and ultimately corrected for the
safety of the athlete(s). Additionally, and similar to other
contemporary debates such as training in a low glycogen
state , without further study potentially elucidating
any maladaptation from consecutive training blocks after
NaHCO3 ingestion, it would be prudent to consider
intermittently incorporating this strategy and only when
certain training outcomes are desired (e.g. maximising
the number of repeated efforts at top speed or high rates
of force output).
Equally important to the performance and training
strategies, however, may be the ingestion protocol used to
systematically introduce NaHCO3. Research is only now
highlighting what practitioners, coaches and athletes
have been aware of for some time, and that is the
inherent variability in individual responsiveness to this
supplement [126–130]. In part, the variability in many
papers may eventuate from the wide range of exercise
tasks and methodological applications, as well as
heterogeneity between participant phenotypes. However, it
may also result from the widespread assumption that
applying mean data on time-to-peak buffering response
rates after NaHCO3 ingestion prior to commencing
exercise is not influencing the outcome of many
performance tasks [128, 129]. Amongst others [131, 132], we
have profiled the ingestion time course of various doses
of NaHCO3 at rest in order to determine peak response
rates (Fig. 1) . Collectively, and variation in ingestion
protocols notwithstanding (e.g. capsules, bolus loads or
dispersed over time), these studies illustrate around
40 min of variation between subjects’ peak in blood
buffering capacity [3, 131, 132]. Indeed, the large degree of
inter-individual variability profiled in Fig. 1 has been
recently observed again but under greater temporal
resolution and in a larger study cohort . Although we
have shown that this variation may not be important for
recreationally trained individuals , we are unaware
of any studies that have documented the variability in
elite athlete populations.
Finally, the origin of the 0.2 to 0.3 g·kg−1 doses in the
sport performance field is somewhat ambiguous.
Although oral ingestion of between 25 to 40 g of NaHCO3
has been documented as early as the 1920s and early
1930s in the clinical literature [124, 134, 135], this range
is believed to have come from the work of Poulus and
colleagues, who in 1974 published the first exercise
performance paper using a physiochemical rationale for
NaHCO3 dose selection . Citing earlier clinical work
[122, 136], these authors determined the amount of
NaHCO3 to be administered intravenously using the
NaHCO3 ¼ subject body weight ðkgÞ
where base deficit (BD) represented the excess of fixed
acid in the blood measured after an incremental cycling
test on a previous visit to the lab and compared to a
standard (pH of 7.4 and a PCO2 of 40 mmHg) . The
0.3 represented a mean weighting factor derived from
the equilibrating time course of NaHCO3 throughout
blood and the extravascular spaces [122, 123]. Of note,
no subsequent studies have included BD in the equation
for determining dose. This, coupled with the variation in
individual weighting factors reported in the original
study (0.25 to 0.38) , warrants further work to
determine whether or not individualising dose
concentrations is necessary, and whether issues related to HCO3−
and [H+] rates of increase and clearance are impacting
upon the efficacy of NaHCO3 as a supplement .
The dosing papers most frequently cited in the sport
performance literature date back to the late 1980s and
early 1990s. These studies looked at a range of
NaHCO3 doses (0.1 to 0.5 g·kg−1) and the relationship
between performance and gastrointestinal (GI)
disturbance, respectively [137, 138]. Collectively, these
studies showed that performance could be improved
when the acute dose ingested ranged between 0.2 and
0.3 g·kg−1, but anything greater was not beneficial and
only induced severe side effects. For the past 30 years,
this dose range has been widely accepted as best
practice. Yet, the small subject numbers (n < 9) coupled
with their non-elite training status (e.g. ‘participating
in athletic events’) of the original research should
warrant further inquiry . This, of course, also
assumes that 0.2 or 0.3 g·kg−1 are the optimal
physiologic doses for maximising blood buffering
In light of the practical issues identified in this
section, we recommend monitoring at an individual level
the time-to-peak rise (and decay ) in [HCO3−] at
doses between 0.2 and 0.3 g·kg−1 and to tailor the
commencement of exercise accordingly . An
individualised loading strategy will also require systematically
incorporating dose distribution and other planned
nutritional interventions, documenting subjective
feedback from the athlete (e.g. gastrointestinal distress
) and monitoring any physiological changes (e.g.
acute weight gain from plasma volume expansion )
observed after ingestion.
We have attempted to provide a comprehensive overview
of the mechanistic links between skeletal muscle fatigue,
metabolic acidosis and NaHCO3 supplementation. The
synergistic associations presented within the ‘Whole-body
Metabolism’, ‘Muscle Physiology’ and ‘Motor Pathways’
sections provide evidence that an integrative perspective is
required to truly understand and optimise the use and
application of this supplement. By consolidating the
mechanistic evidence into one text, we aimed to provide new
insights and possibly direct future research in the field of
NaHCO3 supplementation. Finally, we hope that this
review provides further evidence base to expand and refine
the use of this supplement to improve athletic
performance beyond traditional methods of application.
JS is responsible for the conceptual framework and design, literature review,
manuscript preparation, critical revision and approval of the review. PM, DB
and GS contributed to the manuscript preparation, critical revision and
approval of the review. SG contributed to the literature review, manuscript
preparation, critical revision and approval of the review.
1. Carr AJ , Hopkins WG , Gore CJ . Effects of acute alkalosis and acidosis on performance: a meta-analysis . Sports Med . 2011 ; 41 : 801 - 14 .
2. Peart DJ , Siegler JC , Vince RV . Practical recommendations for coaches and athletes: a meta-analysis of sodium bicarbonate use for athletic performance . J Strength Cond Res . 2012 ; 26 : 1975 - 83 .
3. Siegler JC , Midgley AW , Polman RCJ , Lever R. Effects of various sodium bicarbonate loading protocols on the time-dependent extracellular buffering profile . J Strength Cond Res . 2010 ; 24 : 2551 - 7 .
4. Mcnaughton LR , Siegler J , Midgley A. Ergogenic effects of sodium bicarbonate . Curr Sports Med Rep . 2008 ; 7 : 230 - 6 .
5. Mcnaughton LR , Gough L , Deb S , Bentley D , Sparks SA . Recent developments in the use of sodium bicarbonate as an ergogenic aid . Curr Sports Med Rep . 2016 ; 15 : 233 - 44 .
6. Spriet LL , Matsos CG , Peters SJ , Heigenhauser GJ , Jones NL . Effects of acidosis on rat muscle metabolism and performance during heavy exercise . Am J Physiol . 1985 ; 248 : C337 - 47 .
7. Spriet L , Lindinger M , Mckelvie R , Heigenhauser G , Jones N. Muscle glycogenolysis and H+ concentration during maximal intermittent cycling . J Appl Physiol . 1989 ; 66 : 8 - 13 .
8. Knuth ST , Dave H , Peters JR , Fitts RH . Low cell pH depresses peak power in rat skeletal muscle fibres at both 30 degrees C and 15 degrees C: implications for muscle fatigue . J Physiol . 2006 ; 575 : 887 - 99 .
9. Debold EP , Beck SE , Warshaw DM . Effect of low pH on single skeletal muscle myosin mechanics and kinetics . Am J Physiol Cell Physiol . 2008 ; 295 : C173 - 9 .
10. Westerblad H , Allen DG. Changes of intracellular pH due to repetitive stimulation of single fibres from mouse skeletal muscle . J Physiol . 1992 ; 449 : 49 - 71 .
11. Overgaard K , Højfeldt GW , Nielsen OB . Effects of acidification and increased extracellular potassium on dynamic muscle contractions in isolated rat muscles . J Physiol . 2010 ; 588 : 5065 - 76 .
12. Kent-Braun JA , Fitts RH , Christie A. Skeletal muscle fatigue . Compr Physiol . 2011 ; 2 : 997 - 1044 .
13. Cairns SP , Lindinger MI . Do multiple ionic interactions contribute to skeletal muscle fatigue ? J Physiol . 2008 ; 586 : 4039 - 54 .
14. Debold EP . Recent insights into the molecular basis of muscular fatigue . Med Sci Sports Exerc . 2012 ; 44 : 1440 - 52 .
15. Bishop D , Claudius B. Effects of induced metabolic alkalosis on prolonged intermittent-sprint performance . Med Sci Sports Exerc . 2005 ; 37 : 759 - 67 .
16. Cameron SL , Mclay-Cooke RT , Brown RC , Gray AR , Fairbairn KA . Increased blood pH but not performance with sodium bicarbonate supplementation in elite rugby union players . Int J Sport Nutr Exerc Metab . 2010 ; 20 : 307 - 21 .
17. Price M , Moss P , Rance S. Effects of sodium bicarbonate ingestion on prolonged intermittent exercise . Med Sci Sports Exerc . 2003 ; 35 : 1303 - 8 .
18. Artioli GG , Gualano B , Coelho DF , Benatti FB , Gailey AW , Lancha AH . Does sodium-bicarbonate ingestion improve simulated judo performance? Int J Sport Nutr Exerc Metab . 2007 ; 17 : 206 - 17 .
19. Siegler JC , Hirscher K. Sodium bicarbonate ingestion and boxing performance . J Strength Cond Res . 2010 ; 24 : 103 - 8 .
20. Wu C-L , Shih M-C , Yang C-C , Huang M-H , Chang C-K. Sodium bicarbonate supplementation prevents skilled tennis performance decline after a simulated match . J Int Soc Sports Nutr . 2010 ; 7 : 33 .
21. Kupcis PD , Slater GJ , Pruscino CL , Kemp JG . Influence of sodium bicarbonate on performance and hydration in lightweight rowing . Int J Sports Physiol Perform . 2012 ; 7 : 11 - 8 .
22. Mitchell TH , Abraham G , Wing S , Magder SA , Cosio MG , Deschamps A , et al. Intravenous bicarbonate and sodium chloride both prolong endurance during intense cycle ergometer exercise . Am J Med Sci . 1990 ; 300 : 88 - 97 .
23. Gonzalez NC , Zamagni M , Clancy RL . Effect of alkalosis on maximum oxygen uptake in rats acclimated to simulated altitude . J Appl Physiol . 1991 ; 71 : 1050 - 6 .
24. Kozak-Collins K , Burke ER , Schoene RB . Sodium bicarbonate ingestion does not improve performance in women cyclists . Med Sci Sports Exerc . 1994 ; 26 : 1510 - 5 .
25. Flinn S , Herbert K , Graham K , Siegler JC . Differential effect of metabolic alkalosis and hypoxia on high-intensity cycling performance . J Strength Cond Res . 2014 ; 28 : 2852 - 8 .
26. Dennig H , Talbott JH , Edwards HT , Dill DB . Effect of acidosis and alkalosis upon capacity for work . J Clin Invest . 1931 ; 9 : 601 - 13 .
27. Needham DM . Machina Carnis. Cambridge : Cambridge University Press ; 2009 .
28. Asmussen E , Dobeln WV , Nielsen M. Blood lactate and oxygen debt after exhaustive work at different oxygen tensions . Acta Physiol Scand . 1948 ; 15 : 57 - 62 .
29. Johnson WR , Black DH . Comparison of effects of certain blood alkalinizers and glucose upon competitive endurance performance . J Appl Physiol . 1953 ; 5 : 577 - 8 .
30. Margaria R , Aghemo P , Sassi G. Effect of alkalosis on performance and lactate formation in supramaximal exercise . Int Z Angew Physiol . 1971 ; 29 : 215 - 23 .
31. Simmons RW , Hardt AB . The effect of alkali ingestion on the performance of trained swimmers . J Sports Med Phys Fitness . 1973 ; 13 : 159 - 63 .
32. Jones NL , Sutton JR , Taylor R , Toews CJ . Effect of pH on cardiorespiratory and metabolic responses to exercise . J Appl Physiol Respir Environ Exerc Physiol . 1977 ; 43 : 959 - 64 .
33. Sutton JR , Jones NL , Toews CJ . Effect of pH on muscle glycolysis during exercise . Clin Sci . 1981 ; 61 : 331 - 8 .
34. McCartney N , Heigenhauser GJ , Jones NL . Effects of pH on maximal power output and fatigue during short-term dynamic exercise . J Appl Physiol Respir Environ Exerc Physiol . 1983 ; 55 : 225 - 9 .
35. Kowalchuk JM , Heigenhauser GJ , Jones NL . Effect of pH on metabolic and cardiorespiratory responses during progressive exercise . J Appl Physiol Respir Environ Exerc Physiol . 1984 ; 57 : 1558 - 63 .
36. Wilkes D , Gledhill N , Smyth R. Effect of acute induced metabolic alkalosis on 800-m racing time . Med Sci Sports Exerc . 1983 ; 15 : 277 - 80 .
37. Gao JP , Costill DL , Horswill CA , Park SH. Sodium bicarbonate ingestion improves performance in interval swimming . Eur J Appl Physiol Occup Physiol . 1988 ; 58 : 171 - 4 .
38. McKenzie DC , Coutts KD , Stirling DR , Hoeben HH , Kuzara G . Maximal work production following two levels of artificially induced metabolic alkalosis . J Sports Sci . 1986 ; 4 : 35 - 8 .
39. Goldfinch J , Mc Naughton L , Davies P. Induced metabolic alkalosis and its effects on 400-m racing time . Eur J Appl Physiol Occup Physiol . 1988 ; 57 : 45 - 8 .
40. Costill DL , Verstappen F , Kuipers H , Janssen E , Fink W. Acid-base balance during repeated bouts of exercise: influence of HCO3 . Int J Sports Med . 1984 ; 5 : 228 - 31 .
41. Linderman JK , Gosselink KL . The effects of sodium bicarbonate ingestion on exercise performance . Sports Med . 1994 ; 18 : 75 - 80 .
42. Matson LG , Tran ZV . Effects of sodium bicarbonate ingestion on anaerobic performance: a meta-analytic review . Int J Sport Nutr . 1993 ; 3 : 2 - 28 .
43. George KP , Maclaren DPM . The effect of induced alkalosis and acidosis on endurance running at an intensity corresponding to 4 mM blood lactate . Ergonomics . 1988 ; 31 ( 11 ): 1639 - 45 .
44. Brien DM , McKenzie DC . The effect of induced alkalosis and acidosis on plasma lactate and work output in elite oarsmen . Eur J Appl Physiol Occup Physiol . 1989 ; 58 : 797 - 802 .
45. Lavender G , Bird SR . Effect of sodium bicarbonate ingestion upon repeated sprints . Br J Sports Med . 1989 ; 23 : 41 - 5 .
46. Duncan MJ , Weldon A , Price MJ . The effect of sodium bicarbonate ingestion on back squat and bench press exercise to failure . J Strength Cond Res . 2014 ; 28 : 1358 - 66 .
47. Egger F , Meyer T , Such U , Hecksteden A. Effects of sodium bicarbonate on high-intensity endurance performance in cyclists: a double-blind, randomized cross-over trial . PLoS ONE . 2014 ; 9 : e114729 - 15 .
48. Krustrup P , Ermidis G , Mohr M. Sodium bicarbonate intake improves highintensity intermittent exercise performance in trained young men . J Int Soc Sports Nutr . 2015 ; 1 - 7 .
49. Hobson RM , Harris RC , Martin D , Smith P , Macklin B , Elliott-Sale KJ , et al. Effect of sodium bicarbonate supplementation on 2000-m rowing performance . Int J Sports Physiol Perform . 2014 ; 9 : 139 - 44 .
50. Berger NJA , Mcnaughton LR , Keatley S , Wilkerson DP , Jones AM . Sodium bicarbonate ingestion alters the slow but not the fast phase of VO2 kinetics . Med Sci Sports Exerc . 2006 ; 38 : 1909 - 17 .
51. Zoladz JA , Szkutnik Z , Krzysztof D , Majerczak J , Korzeniewski B. Preexercise metabolic alkalosis induced via bicarbonate ingestion accelerates VO2 kinetics at the onset of a high-power-output exercise in humans . J Appl Physiol . 2004 ; 98 : 895 - 904 .
52. Kolkhorst FW , Rezende RS , Levy SS , Buono MJ . Effects of sodium bicarbonate on VO2 kinetics during heavy exercise . Med Sci Sports Exerc . 2004 ; 36 : 1895 - 9 .
53. Hermansen L , Osnes JB . Blood and muscle pH after maximal exercise in man . J Appl Physiol . 1972 ; 32 : 304 - 8 .
54. Mainwood GW , Worsley-Brown P. The effects of extracellular pH and buffer concentration on the efflux of lactate from frog sartorius muscle . J Physiol . 1975 ; 250 : 1 - 22 .
55. Hirche HJ , Hombach V , Langohr HD , Wacker U , Busse J. Lactic acid permeation rate in working gastrocnemii of dogs during metabolic alkalosis and acidosis . Pflugers Arch Eur J Physiol . 1975 ; 356 : 209 - 22 .
56. Spriet LL , Lindinger MI , Heigenhauser GJ , Jones NL . Effects of alkalosis on skeletal muscle metabolism and performance during exercise . Am J Physiol . 1986 ; 251 : R833 - 9 .
57. Hollidge-Horvat MG , Parolin ML , Wong D , Jones NL , Heigenhauser GJ . Effect of induced metabolic alkalosis on human skeletal muscle metabolism during exercise . Am J Physiol Endocrinol Metab . 2000 ; 278 : E316 - 29 .
58. Granier PL , Dubouchaud H , Mercier BM , Mercier JG , Ahmaidi S , Préfaut CG. Effect of NaHCO3 on lactate kinetics in forearm muscles during leg exercise in man . Med Sci Sports Exerc . 1996 ; 28 : 692 - 7 .
59. Bishop D , Edge J , Thomas C , Mercier J. High-intensity exercise acutely decreases the membrane content of MCT1 and MCT4 and buffer capacity in human skeletal muscle . J Appl Physiol . 2006 ; 102 : 616 - 21 .
60. Raymer GH , Marsh GD , Kowalchuk JM , Thompson RT . Metabolic effects of induced alkalosis during progressive forearm exercise to fatigue . J Appl Physiol . 2004 ; 96 : 2050 - 6 .
61. Forbes SC , Raymer GH , Kowalchuk JM , Marsh GD . NaHCO3-induced alkalosis reduces the phosphocreatine slow component during heavy-intensity forearm exercise . J Appl Physiol . 2005 ; 99 : 1668 - 75 .
62. Churchward-Venne TA , Kowalchuk JM , Marsh GD . Effects of ammonium chloride ingestion on phosphocreatine metabolism during moderate- and heavy-intensity plantar-flexion exercise . Eur J Appl Physiol . 2009 ; 108 : 1189 - 200 .
63. Spriet LL , Soderlund K , Bergstrom M , Hultman E. Skeletal muscle glycogenolysis, glycolysis, and pH during electrical stimulation in men . J Appl Physiol . 1987 ; 62 : 616 - 21 .
64. Spriet LL , Lindinger MI , Mckelvie RS , Heigenhauser GJ , Jones NL . Muscle glycogenolysis and H+ concentration during maximal intermittent cycling . J Appl Physiol . 1989 ; 66 : 8 - 13 .
65. Sostaric SM , Skinner TLSL , Brown MJ , Sangkabutra T , Medved I , Medley T , et al. Alkalosis increases muscle K+ release, but lowers plasma [K+] and delays fatigue during dynamic forearm exercise . J Physiol . 2005 ; 570 : 185 - 205 .
66. Street D , Nielsen JJ , Bangsbo J , Juel C. Metabolic alkalosis reduces exerciseinduced acidosis and potassium accumulation in human skeletal muscle interstitium . J Physiol . 2005 ; 566 : 481 - 9 .
67. Bouissou P , Defer G , Guezennec CY , Estrade PY , Serrurier B. Metabolic and blood catecholamine responses to exercise during alkalosis . Med Sci Sports Exerc . 1988 ; 20 : 228 - 32 .
68. Bird SR , Wiles J , Robbins J. The effect of sodium bicarbonate ingestion on 1500-m racing time . J Sports Sci . 1995 ; 13 : 399 - 403 .
69. van Montfoort MCE , van Dieren L , Hopkins WG , Shearman JP . Effects of ingestion of bicarbonate, citrate, lactate, and chloride on sprint running . Med Sci Sports Exerc . 2004 ; 36 : 1239 - 43 .
70. Lindh A , Peyrebrune M , Ingham S , Bailey D , Folland J. Sodium bicarbonate improves swimming performance . Int J Sports Med . 2008 ; 29 : 519 - 23 .
71. Broch-Lips M , Overgaard K , Praetorius HA , Nielsen OB . Effects of extracellular HCO3 on fatigue, pHi, and K+ efflux in rat skeletal muscles . J Appl Physiol . 2007 ; 103 : 494 - 503 .
72. Sejersted OM , Sjøgaard G. Dynamics and consequences of potassium shifts in skeletal muscle and heart during exercise . Physiol Rev . 2000 ; 80 : 1411 - 81 .
73. Houtman CJ , Heerschap A , Zwarts MJ , Stegeman DF. pH heterogeneity in tibial anterior muscle during isometric activity studied by 31P-NMR spectroscopy . J Appl Physiol . 2001 ; 91 : 191 - 200 .
74. Poulus AJ , Docter HJ , Westra HG . Acid-base balance and subjective feelings of fatigue during physical exercise . Eur J Appl Physiol Occup Physiol . 1974 ; 33 : 207 - 13 .
75. Housh TJ , de Vries HA , Johnson GO , Evans SA , McDowell S. The effect of ammonium chloride and sodium bicarbonate ingestion on the physical working capacity at the fatigue threshold . Eur J Appl Physiol Occup Physiol . 1991 ; 62 : 189 - 92 .
76. Fabiato A , Fabiato F. Effects of pH on the myofilaments and the sarcoplasmic reticulum of skinned cells from cardiace and skeletal muscles . J Physiol . 1978 ; 276 : 233 - 55 .
77. Edman KA , Mattiazzi AR . Effects of fatigue and altered pH on isometric force and velocity of shortening at zero load in frog muscle fibres . J Muscle Res Cell Motil . 1981 ; 2 : 321 - 34 .
78. Robertson SP , Glenn W , Kerrick L. The effects of pH on Ca2+-activated force in frog skeletal muscle fibers . Pflugers Arch Eur J Physiol . 1979 ; 380 : 41 - 5 .
79. Metzger JM , Moss RL . Greater hydrogen ion-induced depression of tension and velocity in skinned single fibres of rat fast than slow muscles . J Physiol . 1987 ; 393 : 727 - 42 .
80. Baldwin KM , Klinkerfuss GH , Terjung RL , Molé PA , Holloszy JO . Respiratory capacity of white, red, and intermediate muscle: adaptative response to exercise . Am J Physiol . 1972 ; 222 : 373 - 8 .
81. Ariano MA , Edgerton VR , Armstrong RB . Hindlimb muscle fiber populations of five mammals . J Histochem Cytochem . 1973 ; 21 : 51 - 5 .
82. Lindinger MI , Heigenhauser GJ , Spriet LL . Effects of alkalosis on muscle ions at rest and with intense exercise . Can J Physiol Pharmacol . 1990 ; 68 : 820 - 9 .
83. Higgins MF , Tallis J , Price MJ , James RS . The effects of elevated levels of sodium bicarbonate (NaHCO3) on the acute power output and time to fatigue of maximally stimulated mouse soleus and EDL muscles . Eur J Appl Physiol . 2012 ; 113 : 1331 - 41 .
84. Inbar O , Rotstein A , Jacobs I , Kaiser P , Dlin R , Dotan R. The effects of alkaline treatment on short‐term maximal exercise . J Sports Sci . 1983 ; 1 : 95 - 104 .
85. Zhang SJ , Bruton JD , Katz A , Westerblad H. Limited oxygen diffusion accelerates fatigue development in mouse skeletal muscle . J Physiol . 2006 ; 572 : 551 - 9 .
86. Benadé AJ , Heisler N. Comparison of efflux rates of hydrogen and lactate ions from isolated muscles in vitro . Respir Physiol . 1978 ; 32 : 369 - 80 .
87. Webster MJ , Webster MN , Crawford RE , Gladden LB . Effect of sodium bicarbonate ingestion on exhaustive resistance exercise performance . Med Sci Sports Exerc . 1993 ; 25 : 960 - 5 .
88. Portington KJ , Pascoe DD , Webster MJ , Anderson LH , Rutland RR , Gladden LB . Effect of induced alkalosis on exhaustive leg press performance . Med Sci Sports Exerc . 1998 ; 30 : 523 - 8 .
89. Edge J , Bishop D , Goodman C. Effects of chronic NaHCO3 ingestion during interval training on changes to muscle buffer capacity, metabolism, and short-term endurance performance . J Appl Physiol . 2006 ; 101 : 918 - 25 .
90. Thomas C , Bishop D , Moore-Morris T , Mercier J. Effects of high-intensity training on MCT1, MCT4, and NBC expressions in rat skeletal muscles: influence of chronic metabolic alkalosis . Am J Physiol Endocrinol Metab . 2007 ; 293 : E916 - 22 .
91. Bishop DJ , Thomas C , Moore-Morris T , Tonkonogi M , Sahlin K , Mercier J. Sodium bicarbonate ingestion prior to training improves mitochondrial adaptations in rats . Am J Physiol Endocrinol Metab . 2010 ; 299 : E225 - 33 .
92. Bouissou P , Estrade PY , Goubel F , Guezennec CY , Serrurier B. Surface EMG power spectrum and intramuscular pH in human vastus lateralis muscle during dynamic exercise . J Appl Physiol . 1989 ; 67 : 1245 - 9 .
93. Hunter AM , De Vito G , Bolger C , Mullany H , Galloway SDR . The effect of induced alkalosis and submaximal cycling on neuromuscular response during sustained isometric contraction . J Sports Sci . 2009 ; 27 : 1261 - 9 .
94. Matsuura R , Arimitsu T , Kimura T , Yunoki T , Yano T. Effect of oral administration of sodium bicarbonate on surface EMG activity during repeated cycling sprints . Eur J Appl Physiol . 2007 ; 101 : 409 - 17 .
95. Yamanaka R , Yunoki T , Arimitsu T , Lian C-S , Yano T. Effects of sodium bicarbonate ingestion on EMG, effort sense and ventilatory response during intense exercise and subsequent active recovery . Eur J Appl Physiol . 2010 ; 111 : 851 - 8 .
96. Lowery M , Nolan P , O'Malley M. Electromyogram median frequency, spectral compression and muscle fibre conduction velocity during sustained sub-maximal contraction of the brachioradialis muscle . J Electromyogr Kinesiol . 2002 ; 12 : 111 - 8 .
97. Siegler JC , Marshall PWM , Raftry S , Brooks C , Dowswell B , Romero R , et al. The differential effect of metabolic alkalosis on maximum force and rate of force development during repeated, high-intensity cycling . J Appl Physiol . 2013 ; 115 : 1634 - 40 .
98. Aagaard P , Simonsen EB , Andersen JL , Magnusson P , Dyhre-Poulsen P. Increased rate of force development and neural drive of human skeletal muscle following resistance training . J Appl Physiol . 2002 ; 93 : 1318 - 26 .
99. Gandevia SC . Spinal and supraspinal factors in human muscle fatigue . Physiol Rev . 2001 ; 81 : 1725 - 89 .
100. Amann M. Central and peripheral fatigue . Med Sci Sports Exerc . 2011 ; 43 : 2039 - 45 .
101. Beekley MD . Carbon dioxide alters the Hoffmann reflex independent of hydrogen ions . Int J Neurosci . 2014 ; 124 : 518 - 23 .
102. Kniffki KD , Schomburg ED , Steffens H. Synaptic responses of lumbar alphamotoneurones to chemical algesic stimulation of skeletal muscle in spinal cats . Brain Res . 1979 ; 160 : 549 - 52 .
103. Rotto DM , Kaufman MP . Effect of metabolic products of muscular contraction on discharge of group III and IV afferents . J Appl Physiol . 1988 ; 64 : 2306 - 13 .
104. Taylor JL , Butler JE , Gandevia SC . Changes in muscle afferents, motoneurons and motor drive during muscle fatigue . Eur J Appl Physiol . 2000 ; 83 : 106 - 15 .
105. Rossi A , Mazzocchio R , Decchi B. Effect of chemically activated fine muscle afferents on spinal recurrent inhibition in humans . Clin Neurophysiol . 2003 ; 114 : 279 - 87 .
106. Fischer M , Schäfer SS . Effects of changes in pH on the afferent impulse activity of isolated cat muscle spindles . Brain Res . 2005 ; 1043 : 163 - 78 .
107. Søgaard K , Gandevia SC , Todd G , Petersen NT , Taylor JL . The effect of sustained low-intensity contractions on supraspinal fatigue in human elbow flexor muscles . J Physiol . 2006 ; 573 : 511 - 23 .
108. Adam A , De Luca CJ . Firing rates of motor units in human vastus lateralis muscle during fatiguing isometric contractions . J Appl Physiol . 2005 ; 99 : 268 - 80 .
109. Dideriksen JL , Enoka RM , Farina D. Neuromuscular adjustments that constrain submaximal EMG amplitude at task failure of sustained isometric contractions . J Appl Physiol . 2011 ; 111 : 485 - 94 .
110. Light AR , Hughen RW , Zhang J , Rainier J , Liu Z , Lee J. Dorsal root ganglion neurons innervating skeletal muscle respond to physiological combinations of protons, ATP, and lactate mediated by ASIC, P2X, and TRPV1 . J Neurophysiol . 2008 ; 100 : 1184 - 201 .
111. Jankowski MP , Rau KK , Ekmann KM , Anderson CE , Koerber HR . Comprehensive phenotyping of group III and IV muscle afferents in mouse . J Neurophysiol . 2013 ; 109 : 2374 - 81 .
112. Pollak KA , Swenson JD , Vanhaitsma TA , Hughen RW , Jo D , White AT , et al. Exogenously applied muscle metabolites synergistically evoke sensations of muscle fatigue and pain in human subjects . Ex Physiol . 2014 ; 99 : 368 - 80 .
113. Amann M , Venturelli M , Ives SJ , McDaniel J , Layec G , Rossman MJ , et al. Peripheral fatigue limits endurance exercise via a sensory feedbackmediated reduction in spinal motoneuronal output . J Appl Physiol . 2013 ; 115 : 355 - 64 .
114. Taylor JL , Petersen N , Butler JE , Gandevia SC . Ischaemia after exercise does not reduce responses of human motoneurones to cortical or corticospinal tract stimulation . J Physiol . 2000 ; 525 : 793 - 801 .
115. Martin PG . Fatigue-sensitive afferents inhibit extensor but not flexor motoneurons in humans . J Neurosci . 2006 ; 26 : 4796 - 802 .
116. Rossman MJ , Garten RS , Venturelli M , Amann M , Richardson RS . The role of active muscle mass in determining the magnitude of peripheral fatigue during dynamic exercise . Am J Physiol Regul Integr Comp Physiol . 2014 ; 306 : R934 - 40 .
117. Siegler JC , Marshall P. The effect of metabolic alkalosis on central and peripheral mechanisms associated with exercise-induced muscle fatigue in humans . Ex Physiol . 2015 ; 100 : 519 - 30 .
118. Van Cutsem M , Duchateau J , Hainaut K. Changes in single motor unit behaviour contribute to the increase in contraction speed after dynamic training in humans . J Physiol . 1998 ; 513 (Pt1): 295 - 305 .
119. Driller MW , Gregory JR , Williams AD , Fell JW . The effects of chronic sodium bicarbonate ingestion and interval training in highly trained rowers . Int J Sport Nutr Exerc Metab . 2013 ; 23 : 40 - 7 .
120. McNaughton L , Backx K , Palmer G , Strange N. Effects of chronic bicarbonate ingestion on the performance of high-intensity work . Eur J Appl Physiol Occup Physiol . 1999 ; 80 : 333 - 6 .
121. Douroudos II , Fatouros IG , Gourgoulis V , Jamurtas AZ , Tsitsios T , Hatzinikolaou A , et al. Dose-related effects of prolonged NaHCO3 ingestion during high-intensity exercise . Med Sci Sports Exerc . 2006 ; 38 : 1746 - 53 .
122. Mellemgaard K , Astrup P. The quantitative determination of surplus amounts of acid or base in the human body . Scand J Clin Lab Invest . 1960 ; 12 : 187 - 99 .
123. Singer RB , Clark JK , Barker ES , Crosley AP , Elkinton JR . The acute effects in man of rapid intravenous infusion of hypertonic sodium bicarbonate solution . I. Changes in acid-base balance and distribution of the excess buffer base . Medicine (Baltimore) . 1955 ; 34 : 51 - 95 .
124. Shock NW , Hastings AB . Studies of the acid-base balance of the blood . J Biol Chem . 1935 ; 112 : 239 - 62 .
125. Hawley JA , Morton JP . Ramping up the signal: promoting endurance training adaptation in skeletal muscle by nutritional manipulation . Clin Exp Pharmacol Physiol . 2014 ; 41 : 608 - 13 .
126. de Araujo Dias FG , da Eira S , de Salles P , Sale C , Artioli GG , Gualano B , et al. (In) consistencies in responses to sodium bicarbonate supplementation: a randomised, repeated measures, counterbalanced and double-blind study . PLoS One . 2015 ; 10 ( 11 ): e0143086 .
127. Saunders B , Sale C , Harris RC , Sunderland C. Sodium bicarbonate and high-intensity-cycling capacity: variability in responses . Int J Sports Physiol Perform . 2014 ; 9 : 627 - 32 .
128. Miller P , Robinson AL , Sparks SA , Bridge CA , Bentley DJ , Mcnaughton LR . The effects of novel ingestion of sodium bicarbonate on repeated sprint ability . J Strength Cond Res . 2016 ; 30 : 561 - 8 .
129. Stannard RL , Stellingwerff T , Artioli GG , Saunders B , Cooper S , Sale C. Doseresponse of sodium bicarbonate ingestion highlights individuality in time course of blood analyte responses . Int J Sport Nutr Exerc Metab . 2016 ; 1 - 20 .
130. Green S , Siegler JC. Empirical modeling of metabolic alkalosis induced by sodium bicarbonate ingestion . Appl Physiol Nutr Metab . 2016 . http://dx.doi. org/10.1123/ijsnem.2015- 0286 .
131. Renfree A. The time course for changes in plasma [H+] after sodium bicarbonate ingestion . Int J Sports Physiol Perform . 2007 ; 2 : 323 - 6 .
132. Price MJ , Singh M. Time course of blood bicarbonate and pH three hours after sodium bicarbonate ingestion . Int J Sports Physiol Perform . 2008 ; 3 : 240 - 2 .
133. Siegler JC , Marshall PWM , Bray J , Towlson C. Sodium bicarbonate supplementation and ingestion timing: does it matter ? J Strength Cond Res . 2012 ; 26 : 1953 - 8 .
134. Koehler AE . The effect of acid and base ingestion upon the acid-base balance . J Biol Chem . 1927 ; 72 : 99 - 122 .
135. Palmer WW , Salvesen H , Jackson HJ. Relationship between the plasma bicarbonate and urinary acidity following the administration of sodium bicarbonate . J Biol Chem . 1920 ; 45 : 101 - 11 .
136. Astrup P , Jørgensen K , Andersen OS , Engel K. The acid-base metabolism. A new approach . Lancet. 1960 ; 1 : 1035 - 9 .
137. Horswill CA , Costill DL , Fink WJ , Flynn MG , Kirwan JP , Mitchell JB , et al. Influence of sodium bicarbonate on sprint performance: relationship to dosage . Med Sci Sports Exerc . 1988 ; 20 : 566 - 9 .
138. Mcnaughton LR . Bicarbonate ingestion: effects of dosage on 60 s cycle ergometry . J Sports Sci . 1992 ; 10 : 415 - 23 .
139. Carr AJ , Slater GJ , Gore CJ , Dawson B , Burke LM . Effect of sodium bicarbonate on [HCO3−], pH, and gastrointestinal symptoms . Int J Sport Nutr Exerc Metab . 2011 ; 2011 : 1 - 6 .