Erratum to: The OECD validation program of the H295R steroidogenesis assay: Phase 3. Final inter-laboratory validation study

Environmental Science and Pollution Research, Oct 2017

In the original article wrong unites were quoted in Table 3 (page 508) and Table 4 (page 510) as well as in the paragraph 3.2 Core chemical exposure experiments on page 509. Also in paragraph 2.3 Selection and testing of chemicals the link to the Supplemental Materials (ESM) was missing.

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Erratum to: The OECD validation program of the H295R steroidogenesis assay: Phase 3. Final inter-laboratory validation study

Erratum to: Environ Sci Pollut Res Erratum to: The OECD validation program of the H295R steroidogenesis assay: Phase 3. Final inter-laboratory validation study Markus Hecker 0 1 2 3 4 5 6 7 8 Henner Hollert 0 1 2 3 4 5 6 7 8 Ralph Cooper 0 1 2 3 4 5 6 7 8 Anne-Marie Vinggaard 0 1 2 3 4 5 6 7 8 Yumi Akahori 0 1 2 3 4 5 6 7 8 Margaret Murphy 0 1 2 3 4 5 6 7 8 Christine Nellemann 0 1 2 3 4 5 6 7 8 Eric Higley 0 1 2 3 4 5 6 7 8 John Giesy 0 1 2 3 4 5 6 7 8 Gary Timm 0 1 2 3 4 5 6 7 8 0 Department of Ecosystem Analysis, Institute for Environmental Research, RWTH Aachen University , 52074 Aachen , Germany 1 Department of Veterinary Biomedical Sciences and Toxicology Centre, University of Saskatchewan , Saskatoon, Saskatchewan , Canada 2 Markus Hecker 3 Office of Science Coordination & Policy, US Environmental Protection Agency , Washington, DC , USA 4 Department of Zoology, University of Heidelberg , 69120 Heidelberg , Germany 5 Senior Environmental Employment Program, National Caucus on Black Aged , Research Triangle Park, NC , USA 6 Department of Biology & Chemistry, City University of Hong Kong , Kowloon, Hong Kong, SAR , China 7 Department of Toxicology & Risk Assessment, National Food Institute, Technical University of Denmark , Soborg , Denmark 8 Endocrinology Branch , RTD, NHEERL, ORD , US Environmental Protection Agency , Research Triangle Park, NC , USA John Newsted 8 & John Laskey 9 & Angela Buckalew 4 & Stefanie Grund 10 & Sibylle Maletz 3 & In the original article wrong unites were quoted in Table 3 (page 508) and Table 4 (page 510) as well as in the paragraph 3.2 Core chemical exposure experiments on page 509. Also in paragraph 2.3 Selection and testing of chemicals the link to the Supplemental Materials (ESM) was missing. The correct versions of the tables and the paragraph as well as the ESM link are provided below. - ENTRIX, Inc., Sasaktoon, SK S7N 5B3, Canada Chemicals Evaluation and Research Institute, Chemicals Assessment Center, Saitama, Japan tion when compared to SCs. The least fold changes were observed for the atrazine exposures where induction of T production was less than 1.5-fold with the exception of Lab 2, at which maximum induction was 2.4-fold. No effect on T production was observed after exposure to atrazine at Lab 6. Exposure to prochloraz resulted in a greater than 15-fold reduction of T production at the greatest concentration tested (100 μM) at all laboratories with the exception of Lab 4 where an up to 4.5-fold reduction was observed. The greater LOEC reported for Lab 2 is likely a function of the relatively great variation among replicate experiments at 0.01 μM (CV=35%). It is unclear why T production by cells was more sensitive to the exposure with prochloraz at Labs 1 and 3. However, a concentration-dependent response was observed starting at 0.01 μM, which is similar to the response patterns at the other labs. Therefore, it cannot be excluded that the significant reduction at 0.0001 and 0.001 μM represents an artifact. Exposure to the other inhibitors resulted in less than 4-fold changes in T production. When chemicals exhibited a less than 1.5-fold change in T production, they were categorized as negatives. This threshold was defined based on the average variation observed across all laboratories among replicate experiments. Some of these negative chemicals could have been categorized as inhibitors in individual cases (molinate: Lab 4; benomyl: Lab 1). However, even in situations where inhibition was observed at an individual laboratory, changes were always less than 2-fold and typically were not concentration-dependent. For instance, exposure to nonoxynol-9 resulted in a decrease in T concentrations at non-cytotoxic concentrations at two of five laboratories for which data was available. Relative to the SCs, inhibition of T production at Lab 1 was 29% (1 μM), while at Lab 2, it was 47% (10 μM). However, it should be noted that, at Lab 2, exposure to 10 μM nonoxynol-9 resulted in an average increase in cell viability (138% viable cells relative to the SCs), and thus the observed reduction in T production may be an artifact due to the correction for cell viability, especially as no such increase was observed by any of the other groups. The greatest letrozole concentration resulted in a significant decrease in T at all laboratories. exposure to the twelve core chemicals. Ranges refer to maximum values measured in repeated experiments. nd – not detectable; — chemical not analyzed. Gray shaded cells – uncertainty due to interference of the antibody based hormone detection system with the test chemical 2nd Labc Max Change 1st Lab 2nd Lab a considered because there was a clear concentration-response at all but the greatest concentration b lead laboratory (Lab 1) c participating laboratory (Labs 2,3 and 4) d Effects occurred at greatest non-cytotoxic concentration; no dose-response 1 10 100d 10 100d 10 1 10 nd nd nd nd nd nd nd nd ⇓⇓⇓ ⇓⇓ ⇓⇓ ⇓⇓ ⇓ ⇓ ⇓⇓⇓ nd nd nd nd nd nd nd ⇑ ⇑ Max Change 1st Lab 2nd Lab ⇓⇓⇓ ⇓⇓⇓ ⇓⇓ ⇓ ⇓⇓ ⇓ ⇓⇓ ⇓⇓ nd nd nd nd nd nd nd nd


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Markus Hecker, Henner Hollert, Ralph Cooper, Anne-Marie Vinggaard, Yumi Akahori, Margaret Murphy, Christine Nellemann, Eric Higley, John Newsted, John Laskey, Angela Buckalew, Stefanie Grund, Sibylle Maletz, John Giesy, Gary Timm. Erratum to: The OECD validation program of the H295R steroidogenesis assay: Phase 3. Final inter-laboratory validation study, Environmental Science and Pollution Research, 2017, 1-3, DOI: 10.1007/s11356-017-0321-7