Improving reproductive performance in overweight/obese women with effective weight management

Human Reproduction Update, May 2004

Obesity and overweight are common conditions in the developed countries and they carry many health consequences, including some reproductive disorders. There is a very high prevalence of obese women in the infertile population and many studies have highlighted the link between obesity and infertility. A large proportion of infertile women have polycystic ovary syndrome (PCOS) which is also linked with increased risk of obesity and other metabolic anomalies. The association between obesity and/or PCOS and hyperinsulinaemia, hyper androgenism and abnormal secretion of other hormones, such as leptin, underlies many reproductive disorders observed in this population. It has been demonstrated that weight loss can improve the fertility of obese women through the recovery of spontaneous ovulation, whereas others will have improved response to ovarian stimulation in infertility treatment. Therefore, it is proposed that following the initial assessment of infertility and body mass index or other measurement of obesity, various weight management interventions, including diet, exercise or pharmacotherapeutic approaches, should be considered for overweight and obese infertile women.

A PDF file should load here. If you do not see its contents the file may be temporarily unavailable at the journal website or you do not have a PDF plug-in installed and enabled in your browser.

Alternatively, you can download the file locally and open with any standalone PDF reader:

Improving reproductive performance in overweight/obese women with effective weight management

Human Reproduction Update Improving reproductive performance in overweight/obese women with effective weight management Robert J.Norman 0 Manny Noakes 1 Ruijin Wu 0 Michael J.Davies 0 Lisa Moran 1 Jim X.Wang 0 0 Reproductive Medicine Unit, University of Adelaide, The Queen Elizabeth Hospital , Woodville Rd, Woodville, SA5011 1 CSIRO Health Sciences and Nutrition , Kintore Ave, Adelaide, SA 5000 , Australia Obesity and overweight are common conditions in the developed countries and they carry many health consequences, including some reproductive disorders. There is a very high prevalence of obese women in the infertile population and many studies have highlighted the link between obesity and infertility. A large proportion of infertile women have polycystic ovary syndrome (PCOS) which is also linked with increased risk of obesity and other metabolic anomalies. The association between obesity and/or PCOS and hyperinsulinaemia, hyperandrogenism and abnormal secretion of other hormones, such as leptin, underlies many reproductive disorders observed in this population. It has been demonstrated that weight loss can improve the fertility of obese women through the recovery of spontaneous ovulation, whereas others will have improved response to ovarian stimulation in infertility treatment. Therefore, it is proposed that following the initial assessment of infertility and body mass index or other measurement of obesity, various weight management interventions, including diet, exercise or pharmacotherapeutic approaches, should be considered for overweight and obese infertile women. fertility ?tness/obese/overweight/PCOS/weight loss Introduction There is great concern at the high prevalence of and the increasing trend to obesity worldwide, especially in Western societies. This is particularly evident in the USA where >50% of all women are overweight and 30% obese. In Australia, 67% of men are overweight or obese and 52% of women are overweight or obese which constitutes a marked increase over the last 20 years (Australian Institute of Health and Welfare, 2002) . The consequent cost of obesity to national health systems is high (Anonymous, 2003) due to the increased morbidity and mortality, including the risk of several cancers associated with obesity (Calle et al., 1999, 2003) . The worldwide trend of increasing obesity is attributable to a combination of reduced exercise, changing dietary composition and increased energy intake. While increased weight gain among young children is particularly evident in developed countries, changing lifestyles in developing countries will see the trend to obesity extend worldwide. Many ethnic groups who either migrate to Western societies or adopt a Western lifestyle are prone to obesity in their changed environment. Diamond (2003) has reviewed the epidemiology of diabetes in various populations; the data suggest that genetic tendencies to obesity are unmasked by geographical differences in food history. The thrifty gene hypothesis postulates the existence of metabolically thrifty genes that permit more ef?cient food utilization, fat deposition and rapid weight gain at occasional times of food abundance, thereby making the gene bearer better able to survive a subsequent famine. An alternative theory of the `thrifty phenotype', based on experimental studies in animals, argues that the capacity for intergenerational metabolic adaptations to increased energy supply are easily exceeded among populations exposed to a `lean' environment for even a single generation (Hales and Ozanne 2003) . Gynaecologists and reproductive scientists have encountered the reproductive consequences of a society increasing in weight as a higher frequency of women diagnosed with disorders of menstruation, infertility, diabetes mellitus in pregnancy and other signi?cant sequelae (Sharpe and Franks, 2002) . In addition, polycystic ovary syndrome (PCOS), is a condition characterized by hyperandrogenism and menstrual disturbances, further complicates the issue (Norman et al., 2002) . At the same time, many advances have been made in recent years on the effect of weight reduction in improving reproductive function in overweight and obese infertile women, and there is now a better understanding of how weight reduction through dieting/exercising leads to improved reproductive performance. Finally, there have been interesting reports of how best to achieve and maintain weight loss through effective weight management. Obesity, PCOS and reproductive disorders Obesity, particularly in women with PCOS, can result in many reproductive disorders. This is due to the complex interaction between the pituitary gland, pancreas and ovary resulting in a changed hormonal secretion pattern (Figure 1) The original descriptions by Stein and Levinthal (1934) emphasized the association of obesity with PCOS. However, the use of hormone measurement and ultrasound led to a realization that not all patients with PCOS suffered from being overweight. Over a third to 50% of PCOS subjects are overweight or obese (Balen et al., 1995; Gambineri et al., 2002) . Variation in body weight between PCOS populations in USA and Europe, attributed to genetic and lifestyle factors, has also been reported recently (Carmina et al., 2003) . In PCOS women of Caucasian origin, it is found that the severity of both metabolic and clinical symptoms is positively correlated with the body mass index (BMI) (Norman et al., 1995) . There is also evidence showing that even normal weight PCOS subjects have increased intra-abdominal fat (Yildirim et al., 2003). In subfertile/infertile women with PCOS, overweight or obesity usually is more prevalent. The relative importance of PCOS status and overweight/obesity in this group of women is yet to be fully understood, although increasing evidence suggests that BMI contributes signi?cantly towards the severity of many problems, 268 such as the risk of miscarriage (Wang et al., 2002) . Understanding the changed glucose metabolism and consequently modi?ed androgen secretion in overweight/obese women with PCOS is the key for assessing the link between obesity and the risk of various reproductive disorders in this group of women. Leptin is another key link between obesity and reproductive disorder. PCOS, insulin resistance and hyperandrogenism Insulin resistance and compensatory hyperinsulinaemia have been consistently documented in lean and obese women with PCOS in comparison to weight-matched controls (Burghen et al., 1980; Chang et al., 1983; Moller and Flier, 1991; Dunaif, 1997) . The severity of insulin resistance is reported to correlate with the severity of the clinical and metabolic phenotype of PCOS (Burghen et al., 1980; Robinson et al., 1993) . Anovulatory women displaying hyperandrogenism and ovarian morphology consistent with PCOS are insulin resistant, whereas ovulatory women usually are not (Dunaif et al., 1985, 1987; Robinson et al., 1993) . As insulin resistance is in?uenced strongly by obesity in nonPCOS subjects (Beard et al., 1987) , it was initially debated whether insulin resistance and hyperinsulinaemia are a primary metabolic disturbance of PCOS or a symptom of the obesity commonly observed in PCOS (Ovesen et al., 1993; Holte et al., 1994a, 1995) . A synergistic interaction appears to exist with a degree of insulin resistance and hyperinsulinaemia in lean PCOS women augmented by the presence of obesity (Campbell and Gerich, 1990; Dunaif, 1997) . The extent of this is still debatable, as not all women with PCOS exhibit hyperinsulinaemia and insulin resistance (Dale et al., 1992; Ehrmann et al., 1995) . Discrepant results may in part be explained by the heterogeneity and complex aetiology of the syndrome, with lean and obese subgroups displaying varying insulin resistance and metabolic pro?les (Acien et al., 1999) . The presence of abdominal obesity is strongly associated with insulin resistance in subjects without PCOS (Hollmann et al., 1997). This additionally appears to hold true for both lean and obese women with PCOS, who may both exhibit increased abdominal obesity (Dale et al., 1992; Bringer et al., 1993) , associated with increased insulin resistance (Pasquali et al., 1994). An alternative mechanism for insulin resistance in PCOS that underlies the risk of developing into type II diabetes has been suggested. De?cient insulin action (Dunaif et al., 1995) , b-cell dysfunction (Ehrmann et al., 1995) , increased insulin secretion in response to dietary stimuli (Holte et al., 1994a,b) and decreased hepatic clearance of insulin (Ciampelli et al., 1997) are hypothesized to play an aetiological role in PCOS. Current research is attempting to elucidate its molecular and genetic rationale; as yet, no clear consensus has been reached. Hyperinsulinaemia correlates positively with the presence of hyperandrogenism in obese and lean women with PCOS. The hyperandrogenism is postulated to result from both increased adrenal and ovarian androgen production (Rosen?eld et al., 1990; Carmina et al., 1992; Ehrmann et al., 1992, 1995) . These tissues do not appear to display insulin resistance whereas ovarian tissues may have selective resistance to insulin (Wu et al., 2003). This would agree with the suggested diverging insulin signalling in ovarian tissue (Nestler et al., 1998) . Androgen overproduction has been reported both in unstimulated and LH-stimulated ovarian thecal cells (Gilling-Smith et al., 1997; Nelson et al., 1999) and in response to hyperinsulinaemia (Poretsky and Kalin, 1987) . Hyperinsulinaemia has additionally been documented as decreasing serum sex hormone-binding globulin (SHBG) levels independent of obesity (Haffner, 1996). SHBG is produced in the liver and helps in the clearance and binding of testosterone. A low SHBG level will thus result in increased bioavailable testosterone (Plymate et al., 1988; Nestler et al., 1991) . While there is also abundant evidence associating an increased BMI with diabetes mellitus, subjects with PCOS in particular have a substantial added risk of glucose intolerance. In a study from Adelaide in women with PCOS (aged 20?30 years), 18% of women with a BMI >30 kg/m2 had impaired glucose metabolism while 15% of women with normal glucose tolerance showed conversion to impaired glucose tolerance or frank diabetes when restudied 5?7 years later (Norman et al., 2001) . The deterioration of glucose metabolism is signi?cantly related to the initial weight (Wang et al., 2003) . Conway et al. (1993) also showed that 8% of lean and 11% of obese women with PCOS had abnormal glucose tolerance. PCOS and metabolic syndrome The endocrine disturbances in PCOS can also result in long-term consequences. The insulin dysfunction previously discussed may lead to an increased occurrence of type II diabetes mellitus and impaired glucose tolerance (IGT) in later adult life (Dahlgren et al., 1992b; Legro et al., 1999) . An increased incidence of hypertension has been reported in PCOS women compared to non-PCOS subjects (Dahlgren et al., 1992b; Holte et al., 1994b; Wild et al., 2000; Elting et al., 2001) . The above attributes are found in both pre- and post-menopausal women (Dahlgren et al., 1992b) , indicating a possible long-term risk associated with PCOS. Together with increased abdominal obesity, this symptom clustering shows a striking similarity to the metabolic syndrome (Reaven, 1988) . The predominance of these risk factors in women with PCOS may place them at a higher risk for cardiovascular and coronary heart disease later in life (Wild et al., 1990; Birdsall et al., 1997) . Dahlgren et al. (1992a) showed in a retrospective study an increased risk pro?le (4?11 fold at ages 40?49 and 50?60 years) of myocardial infarction (MI) in women with PCOS compared to the general population. Increased activity of plasminogen activation inhibitor-I (PAI-I) and C-reactive protein (CRP) associated with increased risk of MI has been reported in PCOS (Sampson et al., 1996). There was also an increased fatal MI rate for women with irregular periods in the Nurses' Health Study (Rich-Edwards et al., 1994) . Conversely, long-term follow-up studies have failed to demonstrate increased circulatory disease mortality with PCOS (Pierpoint et al., 1998) . The lack of a uniform de?nition of PCOS until recently and the heterogeneous nature of this syndrome has limited our capacity to study the real association between PCOS and cardiovascular disease. A large, prospective, long-term follow-up study in a PCOS population with clear and extensive phenotyping of PCOS abnormalities at baseline is needed, as recently pointed out by Legro (2003) . Obesity and leptin Leptin plays a potentially important role in human infertility given the discovery of its regulatory effect on fertility in the mouse (Castracane and Henson, 2002) . There is a strong correlation between serum leptin concentrations and body fat (Maffei et al., 1995; Considine et al., 1996; Vicennati et al., 1998) and BMI (Chapman et al., 1997) in humans. Leptin levels have also been reported to be increased in women with PCOS (Brzechffa et al., 1996; Vicennati et al., 1998) although this was not supported by many other studies (Chapman et al., 1997; Rouru et al., 1997; Gennarelli et al., 1998) . There is no clear explanation for this inconsistency, although the complex interrelation between leptin and body weight, obesity, body fat distribution and many other factors means that studies with large sample size and proper statistical analysis are required for delineating the relationship. On the other hand, there is a consistent positive association between leptin levels and obesity (Brzechffa et al., 1996; Chapman et al., 1997; Rouru et al., 1997; Gennarelli et al., 1998; Vicennati et al., 1998) and non-obese women of PCOS were not hyperleptinaemic (El Orabi et al., 1999). Given the well-established effect of leptin on ovarian steroidogenesis and ovulation in rodents (Duggal et al., 2000; Ryan et al., 2002, 2003) and in humans (Agarwal et al., 1999; Brannian et al., 1999; Lof?er et al., 2001) , it can be speculated that the high concentration of leptin might have a role in the pathogenesis of PCOS and reproductive disorders in?uenced by obesity. Obesity and menstrual disorder Classic studies by Mitchell and Rogers (Mitchell, 1953) and Hartz et al. (1979) showed that obesity was present at a 4-fold higher rate in women with menstrual disturbances than in women with normal cycles. Forty-?ve per cent of amenorrhoeic women were obese whereas only 9?13% of women with normal periods were overweight. Furthermore, anovulation was strongly associated with obesity: grossly obese women had a rate of menstrual disturbance 3.1-fold more frequent than women in the normal weight range (BMI 18.5?25.0 kg/m2). Teenage obesity was positively correlated with menstrual irregularity later in life and obesity was correlated with abnormal and long cycles, heavy menstrual ?ow and hirsutism. Lake et al. (1997) studied women at ages 7, 11, 16, 23 and 33 years and found obesity in childhood and the early 20s increased the risk of menstrual problems [odds ratio (OR) 1.75 and 1.59 respectively]. Women who were overweight (BMI 23.9?28.6 kg/m2) and obese (>28.6 kg/m2) at 23 years of age were respectively 1.32 and 1.75 times more likely to have menstrual dif?culties. Girls with menarche at 9, 10 or 11 years were more likely to have menstrual problems at 16.5 years (OR 1.45 for mild and 1.94 for severe menstrual abnormality), as con?rmed by Ibanez et al. (1998) . The presence of PCOS may further aggravate the effect of obesity on menstrual functions. Of 1741 UK subjects with PCOS, 70% had menstrual disturbances and only 22% had normal menstrual function if their BMI was >30 kg/m2 (Balen et al., 1995) . Kiddy et al. (1992) found that obese subjects with PCOS had an 88% chance of menstrual disturbance compared to 72% in non-obese subjects with PCOS. In the Nurses' Health Study II (n = 101 073 women), women with long or highly irregular menstrual cycles (>40 days length) had a signi?cantly increased relative risk (RR) of developing type II diabetes mellitus compared to women with a regular menstrual cycle (26?31 days) (RR = 2.08) after adjustment for BMI. This risk was more marked in obese women although lean women with menstrual irregularity also had an increased risk of type II diabetes mellitus (RR of 1.67, 1.74 and 3.86 for BMI at age 18 of <25, 25? 29 and >30 respectively) (Solomon et al., 2001) . This indicates that reproductive dysfunction is associated with an increase in metabolic morbidity that is only partially mediated by weight. Obesity and infertility Many multiparous women are obese, indeed most obese women are able to achieve pregnancy readily. In support of this, a large study of fertile women did not show any relationship between conception rates and weight or BMI (Howe et al., 1985) . However, obese and overweight women are over-represented in gynaecological and reproductive medicine clinics. Obesity in the teenage years is more common among married women who never became pregnant than for married women who did become pregnant (Hartz et al., 1979) . The Nurses' Health Study reported that in 2527 married infertile nurses, the relative risk of ovulatory infertility was 1.3-fold higher (95% CI 1.2?1.6) in the group with a BMI range of 24?31 kg/m2 and 2.7-fold higher (2.0?3.7) in women with a BMI >32 kg/m2 (Rich-Edwards et al., 1994) . More recent data from this group show that ovulatory infertility can be largely attributable to overweight and a sedentary lifestyle (Rich-Edwards et al., 2002) . Grodstein et al. (1994a) showed that anovulatory 270 infertility in 1880 infertile women and 4023 controls was more common in those with a BMI of >26.9 kg/m2 (RR 3.1, 2.2?4.4) Even high normal to slightly overweight levels may have an effect on fertility. Weight during childhood did not predict adult fecundity, but weight at 23 years did if the woman was obese (OR 0.69, 0.56? 0.87). Obese women at 23 years were less likely to become pregnant within 12 months than women of normal weight, while infertility rate was 33.6% in obese women versus 18.6% in normal weight women (Lake et al., 1997) . Zaadstra et al. (1993) found that the upper quartile of BMI (>33.1 kg/m2) in a group of apparently normal women who were undergoing donor insemination led to a reduced chance of pregnancy (OR 0.43). This was a particularly signi?cant study because few of the women required medication to stimulate ovulation. Kusakari et al. (1990) in Japan found that obesity was related to anovulation and/or infertility and Balen et al. (1995) also found that obesity was correlated with higher infertility rates. In 204 North American women (Green et al., 1988) , there was a reduced fertility rate among women who were >20% of ideal body weight (OR 2.1)?this did not apply to women who had previously been pregnant. Indeed, obese or overweight subfertile or infertile women have a lower success rate during infertility treatment (Koloszar et al., 2002) . Several reports con?rmed the independent effect of BMI on fecundity in infertile women treated by assisted reproductive technology; with very obese women having half the odds of conception compared to moderate BMI women (Wang et al., 2000; Wittemer et al., 2000; Nichols et al., 2003) although complex interaction between various factors, including infertility aetiology and body fat distribution, may obscure such a relationship (Lashen et al., 1999) . It has been suggested that intrafollicular hCG concentrations are related to BMI, and this may explain the concurrent decrease in embryo quality and pregnancy rates (Carrell et al., 2001) . Imani et al. (2000) found that free androgen index and leptin are the most prominent endocrine predictors of ovarian response during ovulation induction by clomiphene citrate. However, more research will be needed for a better understanding of the association. Women with central obesity take longer to become pregnant, indicating that fat distribution plays a role in the chance of becoming pregnant. Zaadstra et al. (1993) have shown that fertile women with central adiposity take longer to become pregnant than women of the same BMI with peripheral adiposity. Even lean women with PCOS have a signi?cantly higher amount of body fat than controls (Kirchengast and Huber, 2001) . There is also an association between central adiposity, anovulatory cycles and hyperinsulinaemia in adolescent girls born small for gestational age (SGA) (Ibanez et al., 2002). Ibanez et al. found that ovulation was restored following metformin treatment and a reduction in abdominal fat mass. The mechanism of this effect is uncertain but it is well known that increased central fat distribution is associated with a higher level of circulating insulin and other features of the metabolic syndrome. Obesity, miscarriage and other adverse pregnancy outcomes Weight excess is associated with an increased risk of miscarriage. In a study of primiparous women seeking a spontaneous pregnancy (Hamilton-Fairley et al., 1992) , 11% of women with a BMI 19? 24.9 kg/m2, 14% with BMI 25?27.9 kg/m2 and 15% of those >28 kg/m2 miscarried (OR 1, 1.26 and 1.37 respectively). Women >82 kg are more likely to miscarry than thinner women (OR 2.7 for 82?95 kg and 3.4 for >95 kg) (Bohrer and Kemmann, 1987) while even a mild increase in BMI (25?28 kg/m2) leads to a signi?cantly higher risk of pregnancy loss (OR 1.37, 1.18?1.60) following gonadotrophin ovulation induction in some series (HamiltonFairley et al., 1992; Pettigrew and Hamilton-Fairley, 1997) . In pregnancies achieved by assisted reproduction treatment, we also showed a marked increase in the risk of miscarriage in overweight and obese women independent of PCOS (Wang et al., 2002) . Another recent study in women receiving donated oocytes also observed obesity as an independent risk factor for miscarriage (Bellver, 2003) . Obesity is also a risk factor for early pregnancy loss after assisted reproduction treatment (Fedorcsak et al., 2000) , although it was found unrelated to preclinical pregnancy loss (Winter et al., 2002) . The adverse effect of overweight and obesity on pregnancy and obstetric outcome is well known. Some of the American studies on massively obese women indicate high health risks and resultant increased costs to the health system (Galtier-Dereure et al., 2000) , for example cost in increased requirement for infertility treatment (Rich-Edwards et al., 2002) . High pre-pregnancy weight is associated with an increased risk of pregnancy-induced hypertension, toxaemia, gestational diabetes, urinary infection, macrosomia, Caesarean section, and increased hospitalization (Fridstrom et al., 1999; Michlin et al., 2000) . PCOS per se seemed to have little effect on pregnancy outcomes other than increased risk of gestational diabetes (Mikola et al., 2001) , although studies with large sample size are needed to distinguish the effect of PCOS and the confounding effect of overweight and obesity. Obesity and response to infertility treatment Most studies show conclusive evidence that increasing BMI is associated with an increased requirement for clomiphene citrate. In several of these, large doses of clomiphene (up to 200 mg per day) were required to ensure ovulation in the heaviest women (Shepard et al., 1979; Lobo et al., 1982; Friedman and Kim 1985; Dickey et al., 1997) . Doses of gonadotrophins required to induce ovulation are also increased in anovulatory women and those requiring ovarian stimulation for any reason (McClure et al., 1992) . Increased weight and BMI in PCOS lead to impaired response to standard doses of clomiphene citrate, although most obese women with this condition will respond to larger doses (Crosignani et al., 1994) . Fedorcsak et al. (2001) showed that obesity, independent from hyperinsulinaemia, was related to lower oocyte recovery on IVF and increased total FSH requirements for stimulation. A similar observation has been made with gonadotrophin ovulation induction in non-PCOS women (Loh et al., 2002) . Improvement of reproductive function through weight management and dietary intervention Weight loss may impact on reproductive functioning for several reasons, which broadly encompass the effect of a reduction in fat and/or lean tissue mass, related changes in some endocrinological parameters and metabolism and even improvement in self-esteem. The effect of weight loss on reproductive functioning depends on initial body weight and probably the amount of weight lost. Modest weight losses of ~10% in obese women have been demonstrated to be effective in improving hormonal pro?les, menstrual regularity, ovulation, and pregnancy rates (Falsetti et al., 1992; Kumar et al., 1993; Clark et al., 1995; Galletly et al., 1996; Hollmann et al., 1996; Norman and Clark, 1998) . Interventions over as little as 4 weeks with weight losses of 5?10% of initial body weight can reduce hyperandrogenism and circulating insulin (Hamilton-Fairley et al., 1993; Clark et al., 1995; Clark et al., 1998; Huber-Buchholz et al., 1999; Wahrenberg et al., 1999) . In addition, the intergenerational tracking of maternal adiposity through perinatal mechanisms indicates a potential to reduce the risk of obesity in the offspring by controlling obesity in the mother (Foreyt and Poston, 1998) . Weight loss improves insulin resistance and hormone pro?le Attenuating insulin resistance has become a target in normalizing hyperandrogenism and anovulation in PCOS. Weight loss improves insulin sensitivity and short-term reproductive ?tness in overweight women and PCOS subjects and is additionally crucial for improving short- and long-term metabolic health. This can be accomplished through dietary control and exercise with the overall aim of energy expenditure exceeding energy intake over a short or medium period. Caloric restriction improves insulin sensitivity measured through euglycaemic hyperinsulinaemia clamps (Andersen et al., 1995; Holte et al., 1995) , fasting glucose:insulin ratios (Pasquali et al., 1986), homeostasis model assessment (Moran et al., 2003) , oral glucose tolerance test (OGTT)-stimulated insulin (Pasquali et al., 1989; HamiltonFairley et al., 1993; Jakubowicz and Nestler, 1997) and fasting insulin (Kiddy et al., 1989; Botwood et al., 1995; Wahrenberg et al., 1999; Pasquali et al., 2000; Van Dam et al., 2002) . Weight loss in PCOS women also decreases hyperlipidaemia (Andersen et al., 1995; Moran et al., 2003) and ovarian cytochrome P450c17a activity (Jakubowicz and Nestler, 1997) and improves adipocyte lipolysis (Wahrenberg et al., 1999) . Following weight loss, metabolic and endocrine variables were improved to a level similar to that of BMI-matched non-PCOS controls (Holte et al., 1995) , indicating a positive role of dietary treatment in restoring reproductive and metabolic function to overweight women with PCOS. Location of adipose tissue reduction is also important in restoring metabolic and reproductive function (Holte et al., 1995; Huber-Buchholz et al., 1999) . Holte et al. (1995) additionally demonstrated that weight loss in women resulted in reduction of truncal? abdominal fat and that endocrine and metabolic improvements between intervention and control groups were removed after adjusting for truncal?abdominal fat. Weight loss also decreases hyperandrogenism (measured as decreases in free androgen index, free or total testosterone and increases in SHBG) and improves menstrual function, ovulation and fertility (Kiddy et al., 1989; Pasquali et al., 1989; Kiddy et al., 1992; Hamilton-Fairley et al., 1993; Botwood et al., 1995; Holte et al., 1995; Jakubowicz and Nestler, 1997; Van Dam et al., 2002; Moran et al., 2003) . Less consistently documented are changes in LH with reductions (Pasquali et al., 1989), increases (Van Dam et al., 2002) and no changes reported (Kiddy et al., 1992; Holte et al., 1995; Jakubowicz and Nestler, 1997) . Although it is anecdotally reported that women with PCOS have dif?culty in achieving and maintaining weight loss, this has not been speci?cally examined. No differences in weight loss have been observed between subjects with and without PCOS following isocaloric 5000?6000 kJ/day (1190?1428 kcal/day) diets for 2?7 months (Jakubowicz and Nestler, 1997; Pasquali et al., 2000) and in our unpublished study in subjects with (n = 20) and without PCOS (n = 12) over 4 months of energy restriction. This has not been investigated under less restrictive or self-managed dieting regimes. It is suggested that women with PCOS may exhibit possible abnormalities in energy expenditure. While resting energy expenditure (REE) does not differ between women with PCOS and weight-matched controls (Segal and Dunaif, 1990; Robinson et al., 1992) , postprandial thermogenesis (PPT) has been found either not to differ (Segal and Dunaif, 1990) or to be decreased (Robinson et al., 1992) in women with PCOS. Robinson et al. (1992) calculated that this difference in PPT between PCOS and controls would account for a weight gain of 1.9 kg per year if maintained in the long-term. Weight loss improves reproductive functions When fertility is a problem, the primary goal of treatment is to normalize serum androgens and restore reproductive function, simply achieved by reducing insulin resistance through a decrease in weight and abdominal fat. Studies of weight loss through lifestyle modi?cation have indicated that improvements in fertility occur with modest weight loss (~5% of initial body weight) and study-end BMI of >30 kg/m2 (Kiddy et al., 1992; Hollmann et al., 1996) . Crosignani et al. (2003) recently showed that there is a parallel improvement in anthropometric indices, ovarian physiology and fertility rate induced by diet. Foreyt and Poston (1998) also suggested that modest weight losses of ~10% of initial weight are effective in improving hormonal pro?les, menstrual regularity, ovulation, and pregnancy rates. In 11/25 women who responded to weight loss with improvements in menstrual cyclicity (ovulation and menstrual cycle length), a signi?cant reduction in fasting insulin and homeostasis model assessment insulin resistance index (HOMA) was observed compared to subjects who showed no improvement in menstrual cyclicity with weight loss (Moran et al., 2003) . Where PCOS is present, this is consistent with the proposed aetiology of insulin resistance. It would be of great clinical use to be able to identify these subjects prior to commencement of a treatment strategy in order to target successful interventions. Short- and long-term management of weight loss Weight management for women has been vigorously recommended by many authors (Hoeger 2001; Norman et al., 2002) . Short-term weight loss has been achieved in overweight PCOS subjects with very low calorie diets (VLCD) (330?421 kcal/day) (Kiddy et al., 1989; Hamilton-Fairley et al., 1993; Andersen et al., 1995; Wahrenberg et al., 1999; Van Dam et al., 2002) and moderate caloric restriction (1000?1500 kcal/day for 3?6 months) (Pasquali et al., 1986, 1989, 2000; Kiddy et al., 1992; Andersen et al., 1995; Holte et al., 1995; Jakubowicz and Nestler, 1997; Moran et al., 2003) . There is evidence that energy restriction alone, independent of weight loss, improves reproductive parameters (Moran et al., 2003) , which has implications for the management of infertility compared to the longer-term prevention of co-morbidities. 272 Despite the short-term bene?ts of severe caloric restriction, sustained long-term weight loss is more dif?cult to achieve (Wadden 1993) , and if weight is regained the manifestations of PCOS may return. In a review of 17 studies of long-term outcome for dietary treatment of obesity in general populations, Ayyad and Andersen (2000) concluded that ~15% of subjects maintain weight loss (all or 9?11 kg) with success rates of up to 14 years of observation. Although physical activity, behaviour modi?cation and continued support are associated with attenuating weight regain (Ayyad and Andersen, 2000) , it is unclear which dietary strategies are optimal in a free-living situation. Some evidence indicates that weight is maintained more effectively and compliance is increased when an ad libitum low fat, high carbohydrate dietary pattern (~30% of daily energy as fat and 55% as carbohydrate) is followed over longer periods of time, compared to ?xed energy diets. Toubro and Astrup (1998) compared 1 year weight maintenance with an ad libitum low fat, high carbohydrate diet after 13.5 kg initial weight loss in 43 obese adults; 65% of the ad libitum group and 40% of the ?xed energy group maintained a weight loss of >5 kg after 2 years. In a cross-sectional study, Shick et al. (1998) assessed the dietary patterns of 438 subjects from the National Weight Control Registry who maintained a weight loss of 30 kg for 5.1 years. Subjects who successfully maintained weight reported continued consumption of a low energy and low fat diet. A systematic evaluation of six randomized controlled trials using partial meal replacement plans for weight management suggests that these types of interventions can safely and effectively produce signi?cant sustainable weight loss and improve weight-related risk factors of disease (Heyms?eld et al., 2003) . The ef?cacy of this approach in PCOS has not been assessed. Finally, pharmacotherapeutic approaches may also be an option for long-term weight loss maintenance. Sibutramine and orlistat are two weight loss drugs currently approved for obesity treatment, which have been associated with signi?cantly greater weight loss than that seen with dieting alone (Thearle and Aronne, 2003) but there is little work reported using these drugs in the context of improving fertility or infertility treatment, such as assisted reproductive techniques or ovulation induction, so it will not be further discussed here. Using insulin-sensitizing agents Therapeutic use of insulin-sensitizing agents such as metformin, diazoxide, troglitazone and D-chiro-insotol (a mediator of the action of insulin at the receptor level) in the treatment of PCOS have resulted in amelioration of hyperinsulinaemia and hyperandrogenism (Nestler et al., 1989; Velazquez et al., 1994; Dunaif et al., 1996; Nestler et al., 1999; Pasquali et al., 2000; Ibanez et al., 2003) . This provides both support for the link between hyperinsulinaemia and hyperandrogenism and an additional potential pharmaceutical target for improvement of both conditions. It has thus been postulated that hyperinsulinaemia is a key metabolic abnormality in PCOS. Metformin, however, is not effective for grossly obese women (Ehrmann et al., 1997) . Some studies found that metformin induced weight loss (Wong and Wong, 2003) although this remains to be con?rmed (Siraj, 2003) . The use of metformin for infertile women with PCOS receiving infertility treatment has become popular in the last few years. A recent systematic review found that metformin is effective, alone or together with ovulation induction agent, for the ovulation induction of PCOS women (Lord et al., 2003) . Appetite, ghrelin and weight management Discrepancies in appetite regulation may exist. Some investigators have claimed that bulimia is a common ?nding in PCOS, but others have not con?rmed this. There has been much interest in the appetite regulation function by hormone ghrelin (Figure 2). Ghrelin is a 28 amino acid acylated peptide produced primarily by the endocrine cells in the stomach and is secreted into the circulation (Kojima et al., 1999) . It stimulates growth hormone (GH) secretion through its action as an endogenous ligand for the hypothalamic?pituitary growth hormone secretagogue receptor (GHS-R) The GHS-R is widespread throughout the body, indicating multiple roles for ghrelin in addition to its GH-secreting actions. In particular, ghrelin has been implicated as an important regulatory peptide in a number of physiological processes in the brain and the periphery including food intake, body weight regulation and endocrine pancreatic function and glucose metabolism (Muccioli et al., 2002). Ghrelin increases sharply immediately prior to onset of feeding. Once feeding has commenced, plasma ghrelin drops to reach a trough 1?2 h post-meal before returning to baseline values (Cummings et al., 2001, 2002; English et al., 2002) . In obesity, fasting ghrelin is decreased (Tschop et al., 2001) and the postprandial decrease may be impaired (English et al., 2002) , potentially compromising meal termination. Following weight loss, fasting ghrelin increases and the impaired post-meal response normalizes (Cummings et al., 2002) . These data support a potential role of ghrelin in the pathogenesis of human obesity. There is a paucity of data on ghrelin homeostasis in PCOS. Fasting ghrelin is decreased in subjects with PCOS compared to controls in some (Pagotto et al., 2002; Scho? et al., 2002) but not all (Orio et al., 2003) studies. Pagotto et al. (2002) additionally observed no change in fasting plasma ghrelin following 7 months of a hypocaloric diet (1200?1400 kcal/day) for either PCOS (n = 10) or non-PCOS (n = 10) age- and weight-matched subjects (Pagotto et al., 2002) . However, in a pilot study, our group observed higher fasting plasma ghrelin in non-PCOS compared to PCOS overweight subjects and a greater increase in fasting ghrelin for non-PCOS subjects following 16 weeks of weight loss (Moran et al., 2003). There was a greater improvement in postprandial ghrelin response following weight loss for non-PCOS compared to the PCOS subjects (unpublished data). There is thus a suggestion that both fasting and postprandial ghrelin homeostasis is impaired in PCOS and that weight loss may partially restore normal ghrelin homeostasis. This indicates that ghrelin is down-regulated in obesity with the consequence of decreasing satiety signals with feeding, potentially leading to a predisposition to overconsumption for subjects with PCOS. The bene?t of weight management through lifestyle change In some studies, a reduced emphasis was put on caloric restriction while more emphasis was placed on lifestyle changes. These included educating subjects on the adoption of general healthy eating practices in conjunction with moderate amounts of lowintensity exercise (Clark et al., 1995, 1998; Huber-Buchholz et al., 1999) . These principles may be easier to sustain than a lifestyle change and thus are likely to improve lifelong maintenance of a healthy weight. The effect of exercise on improving insulin sensitivity independent of weight loss has also been documented (Goodyear and Kahn, 1998) . Lifestyle changes can also aid in normalizing hyperlipidaemia and hypertension in reducing the risk for cardiovascular disease and diabetes mellitis, including the 25% reduction of fasting insulin and triglycerides and 10% reduction in weight and blood pressure (Noakes et al., 1999) . Longer-term lifestyle studies indicate that improvements in fertility occur with modest weight loss (~5% of initial body weight) and study-end BMI of >30 kg/m2. Clark et al. (1995) conducted a prospective study including a weight loss component to determine whether it could help infertile, overweight, anovulatory women. A weekly programme of behavioural change in relation to exercise and diet for 6 months resulted in an average weight loss of 6.3 kg, a restoration of ovulation in 12 of the 13 subjects and pregnancy in 11 women. Fasting insulin and testosterone concentrations dropped signi?cantly. A further study (Clark et al., 1998) of the same protocol involved 67 anovulatory women in an exercise and dietary intervention for 6 months. Women in the study lost an average of 10.2 kg or 3.7 kg/ m2 (10% reduction of BMI) with 60 of the 67 anovulatory subjects resuming spontaneous ovulation. Of these women, 52 achieved a pregnancy, 45 of which resulted in a live birth. A low fat (~30% of energy and saturated fat ~10% of energy), moderate protein and moderate carbohydrate intake and increased consumption of ?bre, wholegrain breads and cereals and fruit and vegetables in conjunction with moderate regular exercise (30?60 minutes/day) is proposed to aid in weight loss and maintenance both in the general population and in obese infertile women with PCOS (Scalzo, 2000). Furthermore, lifestyle modi?cation through diet and exercise programmes in obese subjects with PCOS improves psychological parameters (self-esteem, anxiety, mean depression scores and scores on general health questionnaire) (Galletly et al., 1996) in addition to reproductive outcomes (Clark et al., 1995, 1998; Huber-Buchholz et al., 1999) . While the effect of long-term weight loss on reduction in diabetic risk has not been studied speci?cally in PCOS, lifestyle change with altered diet and increased physical activity is associated with signi?cant reduction in the risks of developing diabetes mellitus in the general population (Knowler et al., 2002) . Furthermore, these interventions are superior to those of medication such as metformin (Doggrell, 2002; Diabetes Prevention Program Research Group, 2002) . Addressing a number of lifestyle factors can improve long-term reproductive and metabolic health. Exercise aids in management of infertility through reducing insulin resistance (Goodyear and Kahn, 1998) , limiting lean muscle mass loss in weight loss (Garrow and Summerbell, 1995) and aiding in maintenance of a reduced weight (Skender et al., 1996) . Furthermore, combining exercise and dietary intervention together will increase the success of the regime (Skender et al., 1996; Frost et al., 2002) . Systematic reviews indicate that improved weight maintenance is aided enormously by exercise (Fogelholm et al., 2000). Smoking is a major risk factor for female sub-fertility, expressed as time to pregnancy and early pregnancy loss, for pre-term birth and low birthweight in babies (Satcher, 2001; Winter et al., 2002) . High levels of alcohol intake have been associated with reduced fertility and increased risk of spontaneous abortion (Grodstein et al., 1994b) . Cognitive behaviour therapy and reduction of psychosocial stressors can aid both in weight loss and maintenance of the reduced weight (Wing, 1992; Skender et al., 1996) . Attempting weight loss in a group environment additionally provides psychological support (Galletly et al., 1996). Modifying additional factors such as alcohol consumption, smoking, cognitive behaviour therapy and use of a group environment can increase the longterm success and maintenance of weight loss and reproductive and metabolic improvements and has been previously successfully applied (Clark et al., 1995, 1998; Huber-Buchholz et al., 1999) . Dietary interventions: what diet? The most important determinant of dietary intervention for weight loss is energy balance, though for many other reasons various types of diet have been proposed and tested. Most studies have looked at carbohydrate-deplete, fat-depleted diets with caloric restriction as the model for long-term sustained weight loss. However, some consumer support groups promote high protein, low carbohydrate or low glycaemic index (GI) diets as being more effective for therapeutic outcomes in this condition. There is no de?nite evidence to support or refute this approach. Long-term weight loss may be more effective with low fat, high carbohydrate diets comprising unre?ned foods such as whole-grain, pasta, brown rice, etc., but the evidence for this is inconsistent and remarkably limited (Saltzman et al., 2001; Poppitt et al., 2002) . None of these studies have been conducted in women with PCOS. The traditional food pyramid emphasizes a low fat, high carbohydrate diet with secondary emphasis on unre?ned carbohydrate. Recent research recommends actively restricting foods high in glycaemic load such as potatoes and re?ned grain products such as white bread; limiting dairy products to one or two servings a day; replacing unhealthy saturated fat with healthier unsaturated vegetable oils; and emphasizing whole grains, fruits and vegetables (Willett and Stampfer, 2003) . The greater emphasis on a diet lower in glycaemic load through minimizing re?ned carbohydrate and using unsaturated fats more liberally is the key feature of this pyramid, although whether this approach is successful as a strategy to achieve energy restriction has not been directly tested other than in one very short-term (6 day) small trial (Dumesnil et al., 2001) . There are many diets on offer to consumers and few have any scienti?c credibility. However, recent publications have shown that mortality is reduced by adherence to a Mediterranean diet (Hu, 274 2003; Trichopoulou et al., 2003) and the use of high protein, low carbohydrate diets has been shown to be associated with better and more sustained weight loss (Foster et al., 2003). Alternative strategies suggest that increasing dietary protein or reducing dietary glycaemic/load may aid in weight loss. Increasing dietary protein at the expense of carbohydrate is proposed to aid weight loss through an increased satiating and thermogenic effect of protein, but evidence for the latter is limited (Raben, 2002) . It may also increase insulin sensitivity through preserving lean body mass in weight loss. Decreasing dietary glycaemic load is proposed to improve cardiovascular risk pro?le and is additionally proposed to aid weight loss through an increased satiating effect of low GI foods. Dietary protein: the effect of increasing dietary protein in weight loss/weight maintenance Baba et al. (1999) reported a decreased fall in resting energy expenditure (REE) with weight loss in a high protein (HP) compared to a low protein (LP) diet (?555.7 kJ compared to ? 1614.1 kJ). However, Luscombe et al. (2003) showed a similar reduction in REE for both HP and LP diets. This may be explained by the difference in protein intake between the two studies (45% compared to 28% respectively for the HP diets) and we propose to repeat these measurements with an increased protein intake (35? 40% of daily intake). In short-term studies, e.g. 24 h, HP diets increase postprandial thermogenesis (PPT) and REE compared to high carbohydrate or fat diets with equal calories (Robinson et al., 1990; Mikkelsen et al., 2000; Luscombe et al., 2002) . Luscombe et al. (2003) reported a 28% increased thermic effect of feeding for a HP compared to a LP meal, corresponding to a difference of 1.3 kg in weight over 6 months. With the potential for reduced PPT in PCOS (Robinson et al., 1992) , this may lead to signi?cant differences in weight loss or maintenance. We propose that a further increase in dietary protein (35?40% of daily intake) may aid in weight loss and maintenance of a reduced weight by minimizing the normal fall in energy expenditure seen in weight loss. While greater decreases in weight and fat composition were observed with HP compared to LP ad libitum diets (Skov et al., 1999) , no signi?cant differences have been observed in isocaloric diets for weight and body fat (Lean et al., 1997; Luscombe et al., 2002; Moran et al., 2003) . In a prior weight loss study in women with type II diabetes, we observed a greater decrease in total (5.3 versus 2.8 kg) and abdominal (1.3 versus 0.7 kg) fat for a HP compared to a LP diet (Parker et al., 2002) . These studies modestly increased protein (30% of daily intake); a more substantial increase (35?40%) has not yet been explored for >4 weeks. Protein is more satiating than carbohydrate or fat (Poppitt et al., 1998), as shown by a 2000 kJ energy intake difference between ad libitum HP and LP diets (Skov et al., 1999) . This increased satiating effect may aid dietary compliance or weight maintenance, particularly in a free-living situation. Qualitatively Layman et al. (2003) reported a greater level of satiety and satisfaction following 10 weeks of an isocaloric HP (30% protein) compared to a LP (16% protein) diet. Oral and intravenous protein or amino acids stimulate insulin release, both singly and synergistically with glucose (Gannon et al., 1988) . Clinically, Baba et al. (1999) reported no difference in fasting insulin levels between HP and LP diets. However, a 17% decrease in insulin sensitivity was reported for a HP compared to a LP diet (Piatti et al., 1994) . In weight loss, reduction of lean body mass (LBM) has important implications for maintaining metabolic rate and improving insulin sensitivity through increasing skeletal muscle insulin-mediated glucose uptake. While no positive effect of increasing dietary protein on sparing LBM was found (Skov et al., 1999) , Piatti et al. (1994) found a decreased reduction in LBM with increasing dietary protein through an effect of proteolysis and maintenance of whole-body nitrogen levels. Piatti et al. increased dietary protein more severely (45% protein) than Skov et al. (25% protein). Furthermore, an increased ratio of fat/LBM loss was reported with weight loss with an isocaloric HP (30% protein) compared to a LP diet (16% protein) (6.36 compared to 3.92 kg) (Layman et al., 2003) . A prior study also indicated that total LBM was preserved to a greater extent in weight loss in hyperinsulinaemic females on a HP (30% protein) compared to a LP diet (15% protein) (?0.1 kg compared to?1.5 kg) (Farnsworth et al., 2003) . Signi?cant improvements in total cholesterol, triglycerides and low-density lipoprotein cholesterol (LDL-C) are observed in studies with no weight loss (Wolfe and Piche, 1999) for HP compared to LP diets. In overweight women with PCOS, we observed a 10% decrease in high-density lipoprotein (HDL-C) for a LP diet with no change for a HP diet after weight loss (Moran et al., 2003). In weight loss, improvements in total cholesterol/ HDL-C (Moran et al., 2003) , triglycerides (Layman et al., 2003) and total cholesterol and LDL-C (Parker et al., 2002) were reported for isocaloric HP compared to LP diets. For ad libitum LP and HP diets, Skov et al. (1999) demonstrated a reduction in plasma triglycerides for the HP diet with no change for the LP diet. However, these changes in lipids are not consistently reported. Farnsworth et al. (2003) also noted a greater lowering of plasma triglycerides on the HP diet in hyperinsulinaemic women. Glycaemic load: the effect of altering glycaemic load in weight loss/weight maintenance Glycaemic index (GI) is a classi?cation index of carbohydrate foods based on their effects on blood glucose response over 2 h, and glycaemic load (GL) is the product of the GI and the amount of Moderate GI 55?70 Mini Wheats?, Kelloggs Nutrigrain?, Kelloggs Sustain?, Uncle Toby's Vita Brits? Basmati rice, white bread Pontiac variety potato, new potato, sweet potato Banana, pineapple, raisins, rockmelon High GI >70 Sanitarium Puffed Wheat?, Kelloggs Rice Bubbles?, Kelloggs Corn?akes?, Sanitarium Weet Bix? Calrose?, white rice, brown rice, wholemeal bread Baked potato, instant potato Watermelon Cordial, soft drinks, orange juice Popcorn, boiled-type lollies Sucrose (table sugar), honey Lucozade? Jelly beans Glucose, maltose (in malt) dietary carbohydrate (Jenkins et al., 1984) . Table I lists GI grouping of some common types of food. Dietary GI and GL are positively associated with risk of coronary artery disease and type II diabetes (Salmeron et al., 1997; Liu et al., 2000) . Clinical intervention studies have shown that weight maintenance low GI diets may lower daylong glucose levels, glycated haemoglobin (HBA1c), triglyceride and total cholesterol concentrations compared to high GI diets (Brand et al., 1991). The effect of low GI diets on improving insulin sensitivity is more controversial. Jarvi et al. (1999) documented 27% lower fasting insulin after 24 days following a low GI diet compared to a high GI diet. However, no differences in insulin sensitivity were found between the two diets. Reduced GI foods have been shown to result in increased satiety, decreased hunger and lower voluntary food intake in 15/16 single day studies (Brand-Miller et al., 2002) . This has not been investigated in longer-term weight loss settings, but a reduced ad libitum energy intake may also occur (Brand-Miller et al., 2002) . In this scenario, increasing dietary protein will also decrease the GL through decreasing the total amount of carbohydrate. An additive effect may additionally exist between the two factors. A signi?cant recent study (Dumesnil et al., 2001) examined low GI? low fat?HP ad libitum diet compared to the conventional American Heart Association (AHA) moderate protein high carbohydrate diets for the treatment of the atherogenic metabolic risk pro?le over 6 days. This is the ?rst study to examine the combined effects of increasing dietary protein and decreasing dietary GI. The AHA diet was associated with signi?cant increases in hunger and decreases in satiety whereas the low GI?low fat?HP ad libitum diet reduced energy intake by 25%. The low GI?low fat?HP diet was associated with an improved metabolic risk pro?le whereas the AHA diet increased triglycerides by 28% and decreased HDL-C by 10% (Dumesnil et al., 2001) . It is possible that a HP?low GI diet may optimally improve glycaemic control, lipid pro?les and insulin sensitivity compared to other dietary interventions. A HP?low GI diet may also aid in long-term weight loss and maintenance of a reduced weight due to the increased satiating effects of low GI foods compared to other dietary interventions but this has yet to be con?rmed. Summary and recommendation for weight management of infertile patients There is well-established evidence for the detrimental effect of overweight and obesity on women's reproductive function; this is further complicated by the presence of PCOS in many infertile women. In addition, the distribution of body fat is also related to the reduction or even loss of fertility. So far, most research has indicated that overweight and obese conditions lower the concentration of SHBG and increase androgen, insulin and leptin secretion and insulin resistance, leading to hyperinsulinaemia and hyperandrogaenmia. However, there is limited understanding of the details of how these changes affect human reproductive function. On the other hand, weight loss has been shown to improve metabolic function, hormonal pro?le and lead to marked recovery or improvement of reproductive function. Therefore the recommendation for overweight/obese patients with infertility is closely related to the rami?cation of this problem. They should have their height, weight and waist circumference recorded at their ?rst consultation and at regular intervals thereafter. Once the patient has been classi?ed as overweight or obese, then weight management should be offered as a ?rst line treatment option. Dietary intervention and increased physical activity remain the optimal treatment strategy for overweight/obese women with PCOS. A relatively small weight loss (~5 kg) can improve insulin resistance and hyperandrogenism, menstrual function and fertility, and large changes in weight may not be needed to restore reproductive function. Weight loss can also improve long-term metabolic health and realistic and achievable target weight loss goals can be set for women. Obesity and overweight can be treated by a variety of strategies including dietary management, physical activity, behaviour modi?cation, pharmacotherapeutic treatment and surgery. Dietary management with lifestyle modi?cation as an objective should be adopted initially with pharmacological and other interventions reserved for use when weight-loss regimes have proved unsuccessful. Since the overall emphasis is to achieve and maintain a reduced weight, attempts should be made to establish sensible eating patterns and a healthy lifestyle. A number of alternative dietary approaches to the conventional low fat?high carbohydrate regime such as partly modi?ed diets or moderately HP?lower carbohydrate diets which are consistent with a healthy eating plan may assist in maintaining an energy restricted diet. The other lifestyle factors, such as alcohol intake, smoking and psychosocial stressors, should also be addressed. A group environment can provide support for weight loss and maintenance of weight loss. At the same time, it is necessary to tailor intervention to an individual's weight and current dietary and exercise patterns. The use of a dietician is warranted to aid in the evaluation of dietary intake and eating patterns and in individualizing an appropriate dietary approach. Acknowledgements Supported by the University of Adelaide, NHMRC (Australia), CSIRO Human Nutrition and The Reproductive Medicine Unit, University of Adelaide. 276 278 Acien P , Quereda F , Matallin P , Villarroya E , Lopez-Fernandez JA , Acien M , Mauri M and Alfayate R ( 1999 ) Insulin androgens and obesity in women with and without polycystic ovary syndrome: a heterogeneous group of disorders . Fertil Steril 72 , 32 ? 40 . Agarwal SK , Vogel K , Weitsman SR and Magof?n DA ( 1999 ) Leptin antagonizes the insulin-like growth factor-I augmentation of steroidogenesis in granulosa and theca cells of the human ovary . J Clin Endocrinol Metab 84 , 1072 ? 1076 . Andersen P , Selje?ot I, Abdelnoor M , Arnesen H , Dale PO , Lovik A and Birkeland K ( 1995 ) Increased insulin sensitivity and ?brinolytic capacity after dietary intervention in obese women with polycystic ovary syndrome . Metabolism 44 , 611 ? 616 . Anonymous ( 2003 ) About Obesity . International Obesity Task Force (IOTF ) 2003 . Australian Institute of Health and Welfare (AIHW) ( 2002 ) Australia's health 2002: The eighth biannual health report of the AIHW . Australian Institute of Health and Welfare , Canberra. Ayyad C and Andersen T ( 2000 ) Long-term ef?cacy of dietary treatment of obesity: a systematic review of studies published between 1931 and 1999 . Obes Rev 1 , 113 ? 119 . Baba NH , Sawaya S , Torbay N , Habbal Z , Azar S and Hashim SA ( 1999 ) High protein versus high carbohydrate hypoenergetic diet for the treatment of obese hyperinsulinemic subjects . Int J Obes Relat Metab Disord 23 , 1202 ? 1206 . Balen AH , Conway GS , Kaltsas G , Techatrasak K , Manning PJ , West C and Jacobs HS ( 1995 ) Polycystic ovary syndrome: the spectrum of the disorder in 1741 patients . Hum Reprod 10 , 2107 ? 2111 . Beard JC , Ward WK , Halter JB , Wallum BJ and Porte D ( 1987 ) Relationship of islet function to insulin action in human obesity . J Clin Endocrinol Metab 65 , 59 ? 64 . Bellver JEA ( 2003 ) Obesity and the risk of spontaneous abortion after oocyte donation . Fertil Steril 79 , 1136 ? 1140 . Birdsall MA , Farquhar CM and White HD ( 1997 ) Association between polycystic ovaries and extent of coronary artery disease in women having cardiac catheterization . Ann Intern Med 126 , 32 ? 35 . Bohrer M and Kemmann E ( 1987 ) Risk factors for spontaneous abortion in menotropin-treated women . Fertil Steril 48 , 571 ? 575 . Botwood N , Hamilton-Fairley D , Kiddy D , Robinson S and Franks S ( 1995 ) Sex hormone-binding globulin and female reproductive function . J Steroid Biochem Mol Biol 53 , 529 ? 531 . Brand JC , Colagiuri S , Crossman S , Allen A , Roberts DC and Truswell AS ( 1991 ) Low-glycemic index foods improve long-term glycemic control in NIDDM . Diabetes Care 14 , 95 ? 101 . Brand-Miller JC , Holt SH , Pawlak DB and McMillan J ( 2002 ) Glycemic index and obesity . Am J Clin Nutr 76 , 281S ? 285S . Brannian JD , Zhao Y and McElroy M ( 1999 ) Leptin inhibits gonadotrophinstimulated granulosa cell progesterone production by antagonizing insulin action . Hum Reprod 14 , 1445 ? 1448 . Bringer J , Lefebvre P , Boulet F , Grigorescu F , Renard E , Hedon B , Orsetti A and Jaf?ol C ( 1993 ) Body composition and regional fat distribution in polycystic ovarian syndrome. Relationship to hormonal and metabolic pro?les . Ann NY Acad Sci 687 , 115 ? 123 . Brzechffa PR , Jakimiuk AJ , Agarwal SK , Weitsman SR , Buyalos RP and Magof?n DA ( 1996 ) Serum immunoreactive leptin concentrations in women with polycystic ovary syndrome . J Clin Endocrinol Metab 81 , 4166 ? 4169 . Burghen GA , Givens JR and Kitabchi AE ( 1980 ) Correlation of hyperandrogenism with hyperinsulinism in polycystic ovarian disease . J Clin Endocrinol Metab 50 , 113 ? 116 . Calle EE , Thun MJ , Petrelli JM , Rodriguez C and Heath CW Jr ( 1999 ) Bodymass index and mortality in a prospective cohort of U.S. adults . N Engl J Med 341 , 1097 ? 1105 . Calle EE , Rodriguez C , Walker-Thurmond K and Thun MJ ( 2003 ) Overweight, obesity and mortality from cancer in a prospectively studied cohort of U.S. adults . N Engl J Med 348 , 1625 ? 1638 . Campbell PJ and Gerich JE ( 1990 ) Impact of obesity on insulin action in volunteers with normal glucose tolerance: demonstration of a threshold for the adverse effect of obesity . J Clin Endocrinol Metab 70 , 1114 ? 1118 . Carmina E , Koyama T , Chang L , Stanczyk FZ and Lobo RA ( 1992 ) Does ethnicity in?uence the prevalence of adrenal hyperandrogenism and insulin resistance in polycystic ovary syndrome? Am J Obstet Gynecol 167 , 1807 ? 1812 . Carmina E , Legro RS , Stamets K , Lowell J and Lobo RA ( 2003 ) Difference in body weight between American and Italian women with polycystic ovary syndrome: in?uence of the diet . Hum Reprod 18 , 2289 ? 2293 . Carrell DT , Jones KP , Peterson CM , Aoki V , Emery BR and Campbell BR ( 2001 ) Body mass index is inversely related to intrafollicular HCG concentrations, embryo quality and IVF outcome . Reprod Biomed Online 3 , 109 ? 111 . Castracane VD and Henson MC ( 2002 ) When did leptin become a reproductive hormone? Semin Reprod Med 20 , 89 ? 92 . Chang RJ , Nakamura, RM , Judd, HL and Kaplan, SA ( 1983 ) Insulin resistance in nonobese patients with polycystic ovarian disease . J Clin Endocrinol Metab 57 , 356 ? 359 . Chapman IM , Wittert GA and Norman RJ ( 1997 ) Circulating leptin concentrations in polycystic ovary syndrome: relation to anthropometric and metabolic parameters . Clin Endocrinol (Oxf) 46 , 175 ? 181 . Ciampelli M , Fulghesu AM , Cucinelli F , Pavone V , Caruso A , Mancuso S and Lanzone A ( 1997 ) Heterogeneity in beta cell activity, hepatic insulin clearance and peripheral insulin sensitivity in women with polycystic ovary syndrome . Hum Reprod 12 , 1897 ? 1901 . Clark AM , Ledger W , Galletly C , Tomlinson L , Blaney F , Wang X and Norman RJ ( 1995 ) Weight loss results in signi?cant improvement in pregnancy and ovulation rates in anovulatory obese women . Hum Reprod 10 , 2705 ? 2712 . Clark AM , Thornley B , Tomlinson L , Galletley C and Norman RJ ( 1998 ) Weight loss in obese infertile women results in improvement in reproductive outcome for all forms of fertility treatment . Hum Reprod 13 , 1502 ? 1505 . Considine RV , Sinha MK , Heiman ML , Kriauciunas A , Stephens TW , Nyce MR , Ohannesian JP , Marco CC , McKee LJ , Bauer TL et al ( 1996 ) Serum immunoreactive-leptin concentrations in normal-weight and obese humans . N Engl J Med 334 , 292 ? 295 . Conway GS , Clark PM and Wong D ( 1993 ) Hyperinsulinaemia in the polycystic ovary syndrome con?rmed with a speci?c immunoradiometric assay for insulin . Clin Endocrinol (Oxf) 38 , 219 ? 222 . Crosignani PG , Ragni G , Parazzini F , Wyssling H , Lombroso G and Perotti L ( 1994 ) Anthropometric indicators and response to gonadotrophin for ovulation induction . Hum Reprod 9 , 420 ? 423 . Crosignani PG , Colombo M , Vegetti W , Somigliana E , Gessati A and Ragni G ( 2003 ) Overweight and obese anovulatory patients with polycystic ovaries: parallel improvements in anthropometric indices, ovarian physiology and fertility rate induced by diet . Hum Reprod 18 , 1928 ? 1932 . Cummings DE , Purnell JQ , Frayo RS , Schmidova K , Wisse BE and Weigle DS ( 2001 ) A preprandial rise in plasma ghrelin levels suggests a role in meal initiation in humans . Diabetes 50 , 1714 ? 1719 . Cummings DE , Weigle DS , Frayo RS , Breen PA , Ma MK , Dellinger EP and Purnell JQ ( 2002 ) Plasma ghrelin levels after diet-induced weight loss or gastric bypass surgery . N Engl J Med 346 , 1623 ? 1630 . Dahlgren E , Janson PO , Johansson S , Lapidus L and Oden A (1992a) Polycystic ovary syndrome and risk for myocardial infarction. Evaluated from a risk factor model based on a prospective population study of women . Acta Obstet Gynecol Scand 71 , 599 ? 604 . Dahlgren E , Johansson S , Lindstedt G , Knutsson F , Oden A , Janson PO , Mattson LA , Crona N and Lundberg PA ( 1992b) Women with polycystic ovary syndrome wedge resected in 1956 to 1965: a long-term follow-up focusing on natural history and circulating hormones . Fertil Steril 57 , 505 ? 513 . Dale PO , Tanbo T , Vaaler S and Abyholm T ( 1992 ) Body weight, hyperinsulinemia and gonadotropin levels in the polycystic ovarian syndrome: evidence of two distinct populations . Fertil Steril 58 , 487 ? 491 . Diabetes Prevention Program Research Group (DPPRG) ( 2002 ) Reduction in the incidence of type 2 diabetes with lifestyle intervention or metformin . N Engl J Med 346 , 393 ? 403 . Diamond J ( 2003 ) The double puzzle of diabetes . Nature 423 , 599 ? 602 . Dickey RP , Taylor SN , Curole DN , Rye PH , Lu PY and Pyrzak R ( 1997 ) Relationship of clomiphene dose and patient weight to successful treatment . Hum Reprod 12 , 449 ? 453 . Doggrell SA ( 2002 ) Metformin and lifestyle intervention prevent Type 2 diabetes: lifestyle intervention has the greater effect . Expert Opin Pharmacother 3 , 1011 ? 1013 . Duggal PS , Van Der Hoek, KH , Milner, CR , Ryan, NK , Armstrong, DT , Magof?n, DA and Norman, RJ ( 2000 ) The in vivo and in vitro effects of exogenous leptin on ovulation in the rat . Endocrinology 141 , 1971 ? 1976 . Dumesnil JG , Turgeon J , Tremblay A , Poirier P , Gilbert M , Gagnon L , StPierre, S, Garneau C , Lemieux I , Pascot A , Bergeron , J and Despres JP ( 2001 ) Effect of a low-glycaemic index?low-fat?high protein diet on the atherogenic metabolic risk pro?le of abdominally obese men . Br J Nutr 86 , 557 ? 568 . Dunaif A ( 1997 ) Insulin resistance and the polycystic ovary syndrome: mechanism and implications for pathogenesis . Endocr Rev 18 , 774 ? 800 . Dunaif A , Hoffman AR , Scully RE , Flier JS , Longcope C , Levy LJ and Crowley WF ( 1985 ) Clinical, biochemical and ovarian morphologic features in women with acanthosis nigricans and masculinization . Obstet Gynecol 66 , 545 ? 552 . Dunaif A , Graf M , Mandeli J , Laumas V and Dobrjansky A ( 1987 ) Characterization of groups of hyperandrogenic women with acanthosis nigricans, impaired glucose tolerance and/or hyperinsulinemia . J Clin Endocrinol Metab 65 , 499 ? 507 . Dunaif A , Xia J , Book CB , Schenker E and Tang Z ( 1995 ) Excessive insulin receptor serine phosphorylation in cultured ?broblasts and in skeletal muscle. A potential mechanism for insulin resistance in the polycystic ovary syndrome . J Clin Invest 96 , 801 ? 810 . Dunaif A , Scott D , Finegood D , Quintana B and Whitcomb R ( 1996 ) The insulin-sensitizing agent troglitazone improves metabolic and reproductive abnormalities in the polycystic ovary syndrome . J Clin Endocrinol Metab 81 , 3299 ? 3306 . Ehrmann DA , Rosen?eld RL, Barnes RB , Brigell DF and Sheikh Z ( 1992 ) Detection of functional ovarian hyperandrogenism in women with androgen excess . N Engl J Med 327 , 157 ? 162 . Ehrmann DA , Sturis J , Byrne MM , Karrison T , Rosen?eld RL and Polonsky KS ( 1995 ) Insulin secretory defects in polycystic ovary syndrome. Relationship to insulin sensitivity and family history of non-insulindependent diabetes mellitus . J Clin Invest 96 , 520 ? 527 . Ehrmann DA , Cavaghan MK , Imperial J , Sturis J , Rosen ?eld RL and Polonsky KS ( 1997 ) Effects of metformin on insulin secretion, insulin action and ovarian steroidogenesis in women with polycystic ovary syndrome . J Clin Endocrinol Metab 82 , 524 ? 830 . ElOrabi H , Ghalia AA , Khalifa A , Mahfouz H , El Shalkani A and Shoieb N ( 1999 ) Serum leptin as an additional possible pathogenic factor in polycystic ovary syndrome . Clin Biochem 32 , 71 ? 75 . Elting MW , Korsen TJ , Bezemer PD and Schoemaker J ( 2001 ) Prevalence of diabetes mellitus, hypertension and cardiac complaints in a follow-up study of a Dutch PCOS population . Hum Reprod 16 , 556 ? 560 . English PJ , Ghatei MA , Malik IA , Bloom SR and Wilding JP ( 2002 ) Food fails to suppress ghrelin levels in obese humans . J Clin Endocrinol Metab 87 , 2984 . Falsetti L , Pasinetti E , Mazzani MD and Gastaldi A ( 1992 ) Weight loss and menstrual cycle: clinical and endocrinological evaluation . Gynecol Endocrinol 6 , 49 ? 56 . Farnsworth E , Luscombe ND , Noakes M , Wittert G , Argyiou E and Clifton PM ( 2003 ) Effect of a high-protein, energy-restricted diet on body composition, glycemic control and lipid concentrations in overweight and obese hyperinsulinemic men and women . Am J Clin Nutr 78 , 31 ? 39 . Fedorcsak P , Storeng R , Dale PO , Tanbo T and Abyholm T ( 2000 ) Obesity is a risk factor for early pregnancy loss after IVF or ICSI . Acta Obstet Gynecol Scand 79 , 43 ? 48 . Fedorcsak P , Dale PO , Storeng R , Tanbo T and Abyholm T ( 2001 ) The impact of obesity and insulin resistance on the outcome of IVF or ICSI in women with polycystic ovarian syndrome . Hum Reprod 16 , 1086 ? 1091 . Fogelholm M , Kukkonen-Harjula K , Nenonen A and Pasanen M ( 2000 ) Effects of walking training on weight maintenance after a very-lowenergy diet in premenopausal obese women: a randomized controlled trial . Arch Intern Med 160 , 2177 ? 2184 . Foreyt JP and Poston WS ( 1998 ) Obesity: a never-ending cycle? Int J Fertil Women's Med 43 , 111 ? 116 . Foster GD , Wyatt HR , Hill JO , McGuckin BG , Brill C , Mohammed BS , Szapary PO , Rader DJ , Edman JS and Klein S ( 2003 ) A randomized trial of a low-carbohydrate diet for obesity . N Engl J Med 348 , 2082 ? 2090 . Fridstrom M , Nisell H , Sjoblom P and Hillensjo T ( 1999 ) Are women with polycystic ovary syndrome at an increased risk of pregnancy-induced hypertension and/or preeclampsia? Hypertens Pregn 18 , 73 ? 80 . Friedman CI and Kim MH ( 1985 ) Obesity and its effect on reproductive function . Clin Obstet Gynecol 28 , 645 ? 663 . Frost G , Lyons F , Bovill-Taylor C , Carter L , Stuttard J and Dornhorst A ( 2002 ) Intensive lifestyle intervention combined with the choice of pharmacotherapy improves weight loss and cardiac risk factors in the obese . J Hum Nutr Diet 15 , 287 ?295; quiz 297? 299 . Galletly C , Clark A , Tomlinson L and Blaney F ( 1996 ) Improved pregnancy rates for obese, infertile women following a group treatment program. An open pilot study . Gen Hosp Psychiat 18 , 192 ? 195 . Galtier-Dereure F , Boegner C and Bringer J ( 2000 ) Obesity and pregnancy: complications and cost . Am J Clin Nutr 71 , 1242S ? 1248S . Gambineri A , Pelusi C , Vicennati V , Pagotto U and Pasquali R ( 2002 ) Obesity and the polycystic ovary syndrome . Int J Obes Relat Metab Disord 26 , 883 ? 896 . Gannon MC , Nuttall FQ , Neil BJ and Westphal SA ( 1988 ) The insulin and glucose responses to meals of glucose plus various proteins in type II diabetic subjects . Metabolism 37 , 1081 ? 1088 . Garrow JS and Summerbell CD ( 1995 ) Meta-analysis: effect of exercise, with or without dieting, on the body composition of overweight subjects . Eur J Clin Nutr 49 , 1 ? 10 . Gennarelli G , Holte J , Wide L , Berne C and Lithell H ( 1998 ) Is there a role for leptin in the endocrine and metabolic aberrations of polycystic ovary syndrome? Hum Reprod 13 , 535 ? 541 . Gilling-Smith C , Story H , Rogers V and Franks S ( 1997 ) Evidence for a primary abnormality of thecal cell steroidogenesis in the polycystic ovary syndrome . Clin Endocrinol (Oxf) 47 , 93 ? 99 . Goodyear LJ and Kahn BB ( 1998 ) Exercise, glucose transport and insulin sensitivity . Annu Rev Med 49 , 235 ? 261 . Green BB , Weiss NS and Daling JR ( 1988 ) Risk of ovulatory infertility in relation to body weight . Fertil Steril 50 , 721 ? 726 . Grodstein F , Goldman MB and Cramer DW ( 1994a ) Body mass index and ovulatory infertility . Epidemiology 5 , 247 ? 250 . Grodstein F , Goldman MB and Cramer DW ( 1994b) Infertility in women and moderate alcohol use . Am J Public Health 84 , 1429 ? 1432 . Haffner SM ( 1996 ) Sex hormone-binding protein, hyperinsulinemia, insulin resistance and noninsulin-dependent diabetes . Horm Res 45 , 233 ? 237 . Hales CN and Ozanne SE ( 2003 ) The dangerous road of catch-up growth . J Physiol 547 , 5 ? 10 . Hamilton-Fairley D , Kiddy D , Watson H , Paterson C and Franks S ( 1992 ) Association of moderate obesity with a poor pregnancy outcome in women with polycystic ovary syndrome treated with low dose gonadotrophin . Br J Obstet Gynaecol 99 , 128 ? 131 . Hamilton-Fairley D , Kiddy D , Anyaoku V , Koistinen R , Seppala M and Franks S ( 1993 ) Response of sex hormone binding globulin and insulinlike growth factor binding protein-1 to an oral glucose tolerance test in obese women with polycystic ovary syndrome before and after calorie restriction . Clin Endocrinol (Oxf) 39 , 363 ? 367 . Hartz AJ , Barboriak PN , Wong A , Katayama KP and Rimm AA ( 1979 ) The association of obesity with infertility and related menstural abnormalities in women . Int J Obes 3 , 57 ? 73 . Heyms?eld SB , van Mierlo CA, van der Knaap HC , Heo M and Frier HI ( 2003 ) Weight management using a meal replacement strategy: meta and pooling analysis from six studies . Int J Obes Relat Metab Disord 27 , 537 ? 549 . Hoeger K ( 2001 ) Obesity and weight loss in polycystic ovary syndrome . Obstet Gynecol Clin North Am 28 , 85 ?97, vi?vii. Hollmann M , Runnebaum B and Gerhard I ( 1996 ) Effects of weight loss on the hormonal pro?le in obese, infertile women . Hum Reprod 11 , 1884 ? 1891 . Hollmann M , Runnebaum B and Gerhard I ( 1997 ) Impact of waist-hip-ratio and body-mass-index on hormonal and metabolic parameters in young, obese women . Int J Obes Relat Metab Disord 21 , 476 ? 483 . Holte J , Bergh T , Berne C , Berglund L and Lithell H (1994a) Enhanced early insulin response to glucose in relation to insulin resistance in women with polycystic ovary syndrome and normal glucose tolerance . J Clin Endocrinol Metab 78 , 1052 ? 1058 . Holte J , Bergh T , Berne C and Lithell H (1994b) Serum lipoprotein lipid pro?le in women with the polycystic ovary syndrome: relation to anthropometric, endocrine and metabolic variables . Clin Endocrinol (Oxf) 41 , 463 ? 471 . Holte J , Bergh T , Berne C , Wide L and Lithell H ( 1995 ) Restored insulin sensitivity but persistently increased early insulin secretion after weight loss in obese women with polycystic ovary syndrome . J Clin Endocrinol Metab 80 , 2586 ? 2593 . Howe G , Westhoff C , Vessey M and Yeates D ( 1985 ) Effects of age, cigarette smoking and other factors on fertility: ?ndings in a large prospective study . Br Med J (Clin Res Ed) 290 , 1697 ? 700 . Hu FB ( 2003 ) The Mediterranean diet and mortality?olive oil and beyond . N Engl J Med 348 , 2595 ? 2596 . Huber-Buchholz MM , Carey DG and Norman RJ ( 1999 ) Restoration of reproductive potential by lifestyle modi?cation in obese polycystic ovary syndrome: role of insulin sensitivity and luteinizing hormone . J Clin Endocrinol Metab 84 , 1470 ? 1474 . Ibanez L , Ong K , Ferrer A , Amin R , Dunger D and de Zegher F ( 2003 ) Lowdose ?utamide-metformin therapy reverses insulin resistance and reduces fat mass in nonobese adolescents with ovarian hyperandrogenism . J Clin Endocrinol Metab 88 , 2600 ? 2606 . Ibanez L , Potau N , Francois I and de Zegher F ( 1998 ) Precocious pubarche, hyperinsulinism and ovarian hyperandrogenism in girls: relation to reduced fetal growth . J Clin Endocrinol Metab 83 , 3558 ? 3562 . Ibanez L , Potau N , Ferrer A , Rodriguez-Hierro F , Marcos MV and De Zegher F ( 2002 ) Anovulation in eumenorrheic, nonobese adolescent girls born small for gestational age: insulin sensitization induces ovulation, increases lean body mass and reduces abdominal fat excess, dyslipidemia and subclinical hyperandrogenism . J Clin Endocrinol Metab 87 , 5702 ? 5705 . Imani B , Eijkemans MJ , de Jong FH , Payne NN , Bouchard P , Giudice LC and Fauser BC ( 2000 ) Free androgen index and leptin are the most prominent endocrine predictors of ovarian response during clomiphene citrate induction of ovulation in normogonadotropic oligoamenorrheic infertility . J Clin Endocrinol Metab 85 , 676 ? 682 . Jakubowicz DJ and Nestler JE ( 1997 ) 17 alpha-Hydroxyprogesterone responses to leuprolide and serum androgens in obese women with and without polycystic ovary syndrome offer dietary weight loss . J Clin Endocrinol Metab 82 , 556 ? 560 . Jarvi AE , Karlstrom BE , Granfeldt YE , Bjorck IE , Asp NG and Vessby BO ( 1999 ) Improved glycemic control and lipid pro?le and normalized ?brinolytic activity on a low-glycemic index diet in type 2 diabetic patients . Diabetes Care 22 , 10 ? 18 . Jenkins DJ , Wolever TM , Jenkins AL , Josse RG and Wong GS ( 1984 ) The glycaemic response to carbohydrate foods . Lancet 2 , 388 ? 391 . Kiddy DS , Hamilton-Fairley D , Seppala M , Koistinen R , James VH , Reed MJ and Franks S ( 1989 ) Diet-induced changes in sex hormone binding globulin and free testosterone in women with normal or polycystic ovaries: correlation with serum insulin and insulin-like growth factor-I. Clin Endocrinol ( Oxf ) 31 , 757 ? 63 . Kiddy DS , Hamilton-Fairley D , Bush A , Short F , Anyaoku V , Reed MJ and Franks S ( 1992 ) Improvement in endocrine and ovarian function during dietary treatment of obese women with polycystic ovary syndrome . Clin Endocrinol (Oxf) 36 , 105 ? 111 . Kirchengast S and Huber J ( 2001 ) Body composition characteristics and body fat distribution in lean women with polycystic ovary syndrome . Hum Reprod 16 , 1255 ? 1260 . Knowler WC , Barrett-Connor E , Fowler SE , Hamman RF , Lachin JM , Walker EA and Nathan DM ( 2002 ) Reduction in the incidence of type 2 diabetes with lifestyle intervention or metformin . N Engl J Med 346 , 393 ? 403 . Kojima M , Hosoda H , Date Y , Nakazato M , Matsuo H and Kangawa K ( 1999 ) Ghrelin is a growth-hormone-releasing acylated peptide from stomach . Nature 402 , 656 ? 660 . Koloszar S , Daru J , Kereszturi A , Zavaczki Z , Szollosi J and Pal A ( 2002 ) Effect of female body weight on ef?ciency of donor AI . Arch Androl 48 , 323 ? 327 . Kumar A , Mittal S , Buckshee K and Farooq A ( 1993 ) Reproductive functions in obese women . Prog Food Nutr Sci 17 , 89 ? 98 . Kusakari M , Takahashi K , Yoshino K and Kitao M ( 1990 ) Relationship between the delayed-reaction type of LH-RH test and obesity in sterile women with ovulatory disturbances: a preliminary report . Int J Fertil 35 , 14 ? 6 , 21 ? 22 . Lake JK , Power C and Cole TJ ( 1997 ) Child to adult body mass index in the 1958 British birth cohort: associations with parental obesity . Arch Dis Child 77 , 376 ? 381 . Lashen H , Ledger W , Bernal AL and Barlow D ( 1999 ) Extremes of body mass do not adversely affect the outcome of superovulation and in-vitro fertilization . Hum Reprod 14 , 712 ? 715 . Layman DK , Boileau RA , Erickson DJ , Painter JE , Shiue H , Sather C and Christou DD ( 2003 ) A reduced ratio of dietary carbohydrate to protein improves body composition and blood lipid pro?les during weight loss in adult women . J Nutr 133 , 411 ? 417 . Lean ME , Han TS , Prvan T , Richmond PR and Avenell A ( 1997 ) Weight loss with high and low carbohydrate 1200 kcal diets in free living women . Eur J Clin Nutr 51 , 243 ? 248 . Legro RS ( 2003 ) Polycystic ovary syndrome and cardiovascular disease: a premature association? Endocr Rev 24 , 302 ? 312 . Legro RS , Kunselman AR , Dodson WC and Dunaif A ( 1999 ) Prevalence and predictors of risk for type 2 diabetes mellitus and impaired glucose tolerance in polycystic ovary syndrome: a prospective, controlled study in 254 affected women . J Clin Endocrinol Metab 84 , 165 ? 169 . Liu S , Willett WC , Stampfer MJ , Hu FB , Franz M , Sampson L , Hennekens CH and Manson JE ( 2000 ) A prospective study of dietary glycemic load, carbohydrate intake and risk of coronary heart disease in US women . Am J Clin Nutr 71 , 1455 ? 1461 . Lobo RA , Gysler M , March CM , GoebelsmannU and Mishell DR Jr ( 1982 ) Clinical and laboratory predictors of clomiphene response . Fertil Steril 37 , 168 ? 174 . Lof?er S , Aust G , Kohler U and Spanel-Borowski K ( 2001 ) Evidence of leptin expression in normal and polycystic human ovaries . Mol Hum Reprod 7 , 1143 ? 119 . Loh S , Wang JX and Matthews CD ( 2002 ) The in?uence of body mass index, basal FSH and age on the response to gonadotrophin stimulation in nonpolycystic ovarian syndrome patients . Hum Reprod 17 , 1207 ? 1211 . Lord JM , Flight IH and Norman RJ ( 2003 ) Metformin in polycystic ovary syndrome, systematic review and meta-analysis . Br Med J 327 , 951 . Luscombe ND , Clifton PM , Noakes M , Parker B and Wittert G ( 2002 ) Effects of energy-restricted diets containing increased protein on weight loss, resting energy expenditure and the thermic effect of feeding in type 2 diabetes . Diabetes Care 25 , 652 ? 657 . Luscombe ND , Clifton PM , Noakes M , Farnsworth E and Wittert G ( 2003 ) Effect of a high-protein, energy-restricted diet on weight loss and energy expenditure after weight stabilization in hyperinsulinemic subjects . Int J Obes Relat Metab Disord 27 , 582 ? 590 . Maffei M , Halaas J , Ravussin E , Pratley RE , Lee GH , Zhang Y , Fei H , Kim S , Lallone R , Ranganathan S et al ( 1995 ) Leptin levels in human and rodent: measurement of plasma leptin and ob RNA in obese and weight-reduced subjects . Nat Med 1 , 1155 ? 1161 . McClure N , McQuinn B , McDonald J , Kovacs GT , Healy DL and Burger HG ( 1992 ) Body weight, body mass index and age: predictors of menotropin dose and cycle outcome in polycystic ovarian syndrome? Fertil Steril Sep , 622 ? 624 . Michlin R , Oettinger M , Odeh M , Khoury S , Ophir E , Barak M , Wolfson M and Strulov , A ( 2000 ) Maternal obesity and pregnancy outcome . Isr Med Assoc J 2 , 10 ? 13 . Mikkelsen PB , Toubro S and Astrup A ( 2000 ) Effect of fat-reduced diets on 24-h energy expenditure: comparisons between animal protein, vegetable protein and carbohydrate . Am J Clin Nutr 72 , 1135 ? 1141 . Mikola M , Hiilesmaa VV , Halttunen M , Suhonen L and Tiitinen A ( 2001 ) Obstetric outcome in women with polycystic ovarian syndrome . Hum Reprod 16 , 226 ? 229 . Mitchell GW and Rogers J ( 1953 ) The in?uence of weight reduction on amenorrhea in obese women . N Engl J Med 249 , 835 ? 837 . Moller DE and Flier JS ( 1991 ) Insulin resistance?mechanisms, syndromes and implications . N Engl J Med 325 , 938 ? 948 . Moran LJ , Noakes M , Clifton PM , Tomlinson L and Norman RJ ( 2003 ) Dietary composition in restoring reproductive and metabolic physiology in overweight women with polycystic ovary syndrome . J Clin Endocrinol Metab 88 , 812 ? 819 . Muccioli G , Tschop M , Papotti M , Deghenghi R , Heiman M and Ghigo E ( 2002 ) Neuroendocrine and peripheral activities of ghrelin: implications in metabolism and obesity . Eur J Pharmacol 440 , 235 ? 254 . Nelson VL , Legro RS , Strauss JF and McAllister JM ( 1999 ) Augmented androgen production is a stable steroidogenic phenotype of propagated theca cells from polycystic ovaries . Mol Endocrinol 13 , 946 ? 957 . Nestler JE , Barlascini CO , Matt DW , Steingold KA , Plymate SR , Clore JN and Blackard WG ( 1989 ) Suppression of serum insulin by diazoxide reduces serum testosterone levels in obese women with polycystic ovary syndrome . J Clin Endocrinol Metab 68 , 1027 ? 1032 . Nestler JE , Powers LP , Matt DW , Steingold KA , Plymate SR , Rittmaster RS , Clore JN and Blackard WG ( 1991 ) A direct effect of hyperinsulinemia on serum sex hormone-binding globulin levels in obese women with the polycystic ovary syndrome . J Clin Endocrinol Metab 72 , 83 ? 89 . Nestler JE , Jakubowicz DJ , de Vargas AF , Brik C , Quintero N and Medina F ( 1998 ) Insulin stimulates testosterone biosynthesis by human thecal cells from women with polycystic ovary syndrome by activating its own receptor and using inositolglycan mediators as the signal transduction system . J Clin Endocrinol Metab 83 , 2001 ? 2005 . Nestler JE , Jakubowicz DJ , Reamer P , Gunn RD and Allan G ( 1999 ) Ovulatory and metabolic effects of D-chiro-inositol in the polycystic ovary syndrome . N Engl J Med 340 , 1314 ? 1320 . Nichols JE , Crane MM , Higdon HL , Miller PB and Boone WR ( 2003 ) Extremes of body mass index reduce in vitro fertilization pregnancy rates . Fertil Steril 79 , 645 ? 647 . Noakes M , Clifton P and McMurchie T ( 1999 ) The role of diet in cardiovascular health. A review of the evidence . Aust J Nutr Dietet 56 , S3 ? S22 . Norman RJ and Clark AM ( 1998 ) Obesity and reproductive disorders: a review . Reprod Fertil Dev 10 ,55? 63 . Norman RJ , Masters SC , Hague W , Beng C , Pannall P and Wang JX ( 1995 ) Metabolic approaches to the subclassi?cation of polycystic ovary syndrome . Fertil Steril 63 , 329 ? 335 . Norman RJ , Masters L , Milner CR , Wang JX and Davies MJ ( 2001 ) Relative risk of conversion from normoglycaemia to impaired glucose tolerance or non-insulin dependent diabetes mellitus in polycystic ovarian syndrome . Hum Reprod 16 , 1995 ? 1998 . Norman RJ , Davies MJ , Lord J and Moran LJ ( 2002 ) The role of lifestyle modi?cation in polycystic ovary syndrome . Trends Endocrinol Metab 13 , 251 ? 257 . Orio F , Jr, Lucidi P , Palomba S , Tauchmanova L , Cascella T , Russo T , Zullo F , Colao A , Lombardi G and De Feo P ( 2003 ) Circulating ghrelin concentrations in the polycystic ovary syndrome . J Clin Endocrinol Metab 88 , 942 ? 945 . Ovesen P , Moller J , Ingerslev HJ , Jorgensen JO , Mengel A , Schmitz O , Alberti KG and Moller N ( 1993 ) Normal basal and insulin-stimulated fuel metabolism in lean women with the polycystic ovary syndrome . J Clin Endocrinol Metab 77 , 1636 ? 1640 . Pagotto U , Gambineri A , Vicennati V , Heiman ML , Tschop M and Pasquali R ( 2002 ) Plasma ghrelin, obesity and the polycystic ovary syndrome: correlation with insulin resistance and androgen levels . J Clin Endocrinol Metab 87 , 5625 ? 5629 . Parker B , Noakes M , Luscombe N and Clifton P ( 2002 ) Effect of a highprotein, high-monounsaturated fat weight loss diet on glycemic control and lipid levels in type 2 diabetes . Diabetes Care 25 , 425 ? 430 . Pasquali R , Fabbri R , Venturoli S , Paradisi R , Antenucci D and Melchionda N ( 1986 ) Effect of weight loss and antiandrogenic therapy on sex hormone blood levels and insulin resistance in obese patients with polycystic ovaries . Am J Obstet Gynecol 154 , 139 ? 144 . Pasquali R , Antenucci D , Casimirri F , Venturoli S , Paradisi R , Fabbri R , Balestra V , Melchionda N and Barbara L ( 1989 ) Clinical and hormonal characteristics of obese amenorrheic hyperandrogenic women before and after weight loss . J Clin Endocrinol Metab 68 , 173 ? 179 . Pasquali R , Casimirri F , Venturoli S , Antonio M , Morselli L , Reho S , Pezzoli A and Paradisi R ( 1994 ) Body fat distribution has weight-independent effects on clinical, hormonal and metabolic features of women with polycystic ovary syndrome . Metabolism 43 , 706 ? 7013 . Pasquali R , Gambineri A , Biscotti D , Vicennati V , Gagliardi L , Colitta D , Fiorini S , Cognigni GE , Filicori M and Morselli-Labate AM ( 2000 ) Effect of long-term treatment with metformin added to hypocaloric diet on body composition, fat distribution and androgen and insulin levels in abdominally obese women with and without the polycystic ovary syndrome . J Clin Endocrinol Metab 85 , 2767 ? 2774 . Pettigrew R and Hamilton-Fairley D ( 1997 ) Obesity and female reproductive function . Br Med Bull 53 , 341 ? 358 . Piatti PM , Monti F , Fermo I , Baruffaldi L , Nasser R , Santambrogio G , Librenti MC , Galli-Kienle M , Pontiroli AE and Pozza G ( 1994 ) Hypocaloric highprotein diet improves glucose oxidation and spares lean body mass: comparison to hypocaloric high-carbohydrate diet . Metabolism 43 , 1481 ? 1487 . Pierpoint T , McKeigue PM , Isaacs AJ , Wild SH and Jacobs HS ( 1998 ) Mortality of women with polycystic ovary syndrome at long-term followup . J Clin Epidemiol 51 , 581 ? 586 . Plymate SR , Matej LA , Jones RE and Friedl KE ( 1988 ) Inhibition of sex hormone-binding globulin production in the human hepatoma (Hep G2) cell line by insulin and prolactin . J Clin Endocrinol Metab 67 , 460 ? 464 . Poppitt SD , McCormack D and Buffenstein R ( 1998 ) Short-term effects of macronutrient preloads on appetite and energy intake in lean women . Physiol Behav 64 , 279 ? 85 . Poppitt SD , Keogh GF , Prentice AM , Williams DE , Sonnemans HM , Valk EE , Robinson E and Wareham NJ ( 2002 ) Long-term effects of ad libitum lowfat, high-carbohydrate diets on body weight and serum lipids in overweight subjects with metabolic syndrome . Am J Clin Nutr 75 , 11 ? 20 . Poretsky L and Kalin MF ( 1987 ) The gonadotropic function of insulin . Endocr Rev 8 , 132 ? 141 . Raben A ( 2002 ) Should obese patients be counselled to follow a lowglycaemic index diet? No. Obes Rev 3 , 245 ? 256 . Reaven GM ( 1988 ) Banting lecture 1988 . Role of insulin resistance in human disease . Diabetes 37 , 1595 ? 1607 . Rich-Edwards JW , Goldman MB , Willett WC , Hunter DJ , Stampfer MJ , Colditz GA and Manson JE ( 1994 ) Adolescent body mass index and infertility caused by ovulatory disorder . Am J Obstet Gynecol 171 , 171 ? 177 . Rich-Edwards JW , Spiegelman D , Garland M , Hertzmark E , Hunter DJ , Colditz GA , Willett WC , Wand H and Manson JE ( 2002 ) Physical activity, body mass index and ovulatory disorder infertility . Epidemiology 13 , 184 ? 190 . Robinson S , ChanSP, Spacey S , Anyaoku V , Johnston DG and Franks S ( 1992 ) Postprandial thermogenesis is reduced in polycystic ovary syndrome and is associated with increased insulin resistance . Clin Endocrinol (Oxf) 36 , 537 ? 543 . Robinson S , Kiddy D , Gelding SV , Willis D , Niththyananthan R , Bush A , Johnston DG and Franks S ( 1993 ) The relationship of insulin insensitivity to menstrual pattern in women with hyperandrogenism and polycystic ovaries . Clin Endocrinol (Oxf) 39 , 351 ? 355 . Robinson SM , Jaccard C , Persaud C , Jackson AA , Jequier E and Schutz Y ( 1990 ) Protein turnover and thermogenesis in response to high-protein and high-carbohydrate feeding in men . Am J Clin Nutr 52 , 72 ? 80 . Rosen?eld RL , Barnes RB , Cara JF and Lucky AW ( 1990 ) Dysregulation of cytochrome P450c 17 alpha as the cause of polycystic ovarian syndrome . Fertil Steril 53 , 785 ? 791 . Rouru J , Anttila L , Koskinen P , Penttila TA , Irjala K , Huupponen R and Koulu M ( 1997 ) Serum leptin concentrations in women with polycystic ovary syndrome . J Clin Endocrinol Metab 82 , 1697 ? 1700 . Ryan NK , Woodhouse CM , Van der Hoek KH , Gilchrist RB , Armstrong DT and Norman RJ ( 2002 ) Expression of leptin and its receptor in the murine ovary: possible role in the regulation of oocyte maturation . Biol Reprod 66 , 1548 ? 1554 . Ryan NK , Van der Hoek KH , Robertson SA and Norman RJ ( 2003 ) Leptin and leptin receptor expression in the rat ovary . Endocrinology 144 , 5006 ? 5013 . Salmeron J , Ascherio A , Rimm EB , Colditz GA , Spiegelman D , Jenkins DJ , Stampfer MJ , Wing AL and Willett WC ( 1997 ) Dietary ?ber, glycemic load and risk of NIDDM in men . Diabetes Care 20 , 545 ? 550 . Saltzman E , Moriguti JC , Das SK , Corrales A , Fuss P , Greenberg AS and Roberts SB ( 2001 ) Effects of a cereal rich in soluble ?ber on body composition and dietary compliance during consumption of a hypocaloric diet . J Am Coll Nutr 20 , 50 ? 57 . Sampson M , Kong C , Patel A , Unwin R and Jacobs HS ( 1996 ) Ambulatory blood pressure pro?les and plasminogen activator inhibitor (PAI-1) activity in lean women with and without the polycystic ovary syndrome . Clin Endocrinol (Oxf) 45 , 623 ? 629 . Satcher D ( 2001 ) Women and Smoking: A Report of the Surgeon General . Atlanta, GA., Centres for Disease Control and Prevention. Scalzo K ( 2000 ) Case problem: dietary recommendations to combat obesity, insulin resistance and other concerns related to polycystic ovary syndrome . J Am Dietet Assoc 100 , 955 ?957; discussion 957? 960 . Scho ? C, Horn R , Schill T , Schlosser HW , Muller MJ and Brabant G ( 2002 ) Circulating ghrelin levels in patients with polycystic ovary syndrome . J Clin Endocrinol Metab 87 , 4607 ? 4610 . Segal KR and Dunaif A ( 1990 ) Resting metabolic rate and postprandial thermogenesis in polycystic ovarian syndrome . Int J Obes 14 , 559 ? 567 . Sharpe RM and Franks S ( 2002 ) Environment, lifestyle and infertility?an inter-generational issue . Nature Cell Biol 4 ( Suppl ), s33 ? 40 . Shepard MK , Balmaceda JP and Leija CG ( 1979 ) Relationship of weight to successful induction of ovulation with clomiphene citrate . Fertil Steril 32 , 641 ? 645 . Shick SM , Wing RR , Klem ML , McGuire MT , Hill JO and Seagle H ( 1998 ) Persons successful at long-term weight loss and maintenance continue to consume a low-energy, low-fat diet . J Am Diet Assoc 98 , 408 ? 413 . Siraj ES ( 2003 ) Is there a role for metformin or acarbose as a weight-loss agent in the absence of diabetes? Cleve Clin J Med 70 , 702 ? 704 . Skender ML , Goodrick GK , Del Junco DJ , Reeves RS , Darnell L , Gotto AM and Foreyt JP ( 1996 ) Comparison of 2-year weight loss trends in behavioral treatments of obesity: diet, exercise and combination interventions . J Am Diet Assoc 96 , 342 ? 346 . Skov AR , Toubro S , Ronn B , Holm L and Astrup A ( 1999 ) Randomized trial on protein versus carbohydrate in ad libitum fat reduced diet for the treatment of obesity . Int J Obes Relat Metab Disord 23 , 528 ? 536 . Solomon CG , Hu FB , Dunaif A , Rich-Edwards J , Willett WC , Hunter DJ , Colditz GA , Speizer FE and Manson JE ( 2001 ) Long or highly irregular menstrual cycles as a marker for risk of type 2 diabetes mellitus . J Am Med Assoc 286 , 2421 ? 2426 . Stein IF and Levinthal ML ( 1935 ) Amenorrhea associated with bilateral polycystic ovaries . Am J Obstet Gynecol 29 , 181 ? 191 . Thearle M and Aronne LJ ( 2003 ) Obesity and pharmacologic therapy . Endocrinol Metab Clin North Am 32 , 1005 ? 1024 . Toubro S and Astrup AV ( 1998 ) [A randomized comparison of two weightreducing diets. Calorie counting versus low-fat carbohydrate-rich ad libitum diet] . Ugeskr Laeger 160 , 816 ? 820 . Trichopoulou A , Costacou T , Bamia C and Trichopoulos D ( 2003 ) Adherence to a Mediterranean diet and survival in a Greek population . N Engl J Med 348 , 2599 ? 2608 . Tschop M , Weyer C , Tataranni PA , Devanarayan V , Ravussin E and Heiman ML ( 2001 ) Circulating ghrelin levels are decreased in human obesity . Diabetes 50 , 707 ? 709 . VanDam EW , Roelfsema F , Veldhuis JD , Helmerhorst FM , Frolich M , Meinders AE , Krans HM and Pijl H ( 2002 ) Increase in daily LH secretion in response to short-term calorie restriction in obese women with PCOS . Am J Physiol Endocrinol Metab 282 , E865 ? 872 . Velazquez EM , Mendoza S , Hamer T , Sosa F and Glueck CJ ( 1994 ) Metformin therapy in polycystic ovary syndrome reduces hyperinsulinemia, insulin resistance, hyperandrogenemia and systolic blood pressure, while facilitating normal menses and pregnancy . Metabolism 43 , 647 ? 654 . Vicennati V , Gambineri A , Calzoni F , Casimirri F , Macor C , Vettor R and Pasquali R ( 1998 ) Serum leptin in obese women with polycystic ovary syndrome is correlated with body weight and fat distribution but not with androgen and insulin levels . Metabolism 47 , 988 ? 992 . Wadden TA ( 1993 ) Treatment of obesity by moderate and severe caloric restriction . Results of clinical research trials. Ann Intern Med 119 , 688 ? 693 . Wahrenberg H , Ek I , Reynisdottir S , Carlstrom K , Bergqvist A and Arner P ( 1999 ) Divergent effects of weight reduction and oral anticonception treatment on adrenergic lipolysis regulation in obese women with the polycystic ovary syndrome . J Clin Endocrinol Metab 84 , 2182 ? 2187 . Wang JX , Davies M and Norman RJ ( 2000 ) Body mass and probability of pregnancy during assisted reproduction treatment: retrospective study . Br Med J 321 , 1320 ? 1321 . Wang JX , Davies MJ and Norman RJ ( 2002 ) Obesity increases the risk of spontaneous abortion during infertility treatment . Obes Res 10 , 551 ? 554 . Wang JX , Norman RJ and Davies MJ ( 2003 ) Contributing factors to the deterioration of glucose metabolism in women with PCOS. PCOS: Current Concepts, Treatment and Ovulation Induction, Part of the proceeding of a serono international symposium, organised by Faculty of Mecicine, Antalya , Turkey. Wild RA , Grubb B , Hartz A , Van Nort JJ , Bachman W and Bartholomew M ( 1990 ) Clinical signs of androgen excess as risk factors for coronary artery disease . Fertil Steril 54 , 255 ? 259 . Wild S , Pierpoint T , McKeigue P and Jacobs H ( 2000 ) Cardiovascular disease in women with polycystic ovary syndrome at long-term follow-up: a retrospective cohort study . Clin Endocrinol (Oxf) 52 , 595 ? 600 . Willett WC and Stampfer MJ ( 2003 ) Rebuilding the food pyramid . Sci Am 288 , 64 ? 71 . Wing RR ( 1992 ) Behavioral treatment of severe obesity . Am J Clin Nutr 55 , 545S ? 551S . Winter E , Wang J , Davies MJ and Norman R ( 2002 ) Early pregnancy loss following assisted reproductive technology treatment . Hum Reprod 17 , 3220 ? 3223 . Wittemer C , Ohl J , Bailly M , Bettahar-Lebugle K and Nisand I ( 2000 ) Does body mass index of infertile women have an impact on IVF procedure and outcome? J Assist Reprod Genet 17 , 547 ? 552 . Wolfe BM and Piche LA ( 1999 ) Replacement of carbohydrate by protein in a conventional-fat diet reduces cholesterol and triglyceride concentrations in healthy normolipidemic subjects . Clin Invest Med 22 , 140 ? 148 . Wong LL and Wong TC ( 2003 ) Metformin induced anorexia and weight loss . Hawaii Med J 62 , 104 ? 105 . Wu XK , Zhou SY , Liu JX , Pollanen P , Sallinen K , Makinen M and Erkkola R ( 2003 ) Selective ovary resistance to insulin signaling in women with polycystic ovary syndrome . Fertil Steril 80 , 954 ? 965 . Yildirim B , Sabir N and Kaleli B ( 2003 ) Relation of intra-abdominal fat distribution to metabolic disorders in nonobese patients with polycystic ovary syndrome . Fertil Steril 79 , 1358 ? 1364 . Zaadstra BM , Seidell JC , Van Noord PA , te Velde ER , Habbema JD , Vrieswijk B and Karbaat J ( 1993 ) Fat and female fecundity: prospective study of effect of body fat distribution on conception rates . Br Med J 306 , 484 ? 487 .

This is a preview of a remote PDF:

Norman, Robert J., Noakes, Manny, Wu, Ruijin, Davies, Michael J., Moran, Lisa, Wang, Jim X.. Improving reproductive performance in overweight/obese women with effective weight management, Human Reproduction Update, 2004, 267-280, DOI: 10.1093/humupd/dmh018