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103 papers found.
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Comparative analysis of fecal microbial communities in cattle and Bactrian camels

Bactrian camels may have a unique gastrointestinal (GI) microbiome because of their distinctive digestive systems, unique eating habits and extreme living conditions. However, understanding of the microbial communities in the Bactrian camel GI tract is still limited. In this study, microbial communities were investigated by comparative analyses of 16S rRNA hypervariable region V4...

Mining Temporal Protein Complex Based on the Dynamic PIN Weighted with Connected Affinity and Gene Co-Expression

The identification of temporal protein complexes would make great contribution to our knowledge of the dynamic organization characteristics in protein interaction networks (PINs). Recent studies have focused on integrating gene expression data into static PIN to construct dynamic PIN which reveals the dynamic evolutionary procedure of protein interactions, but they fail in...

△-Convergence analysis of improved Kuhfittig iterative for asymptotically nonexpansive nonself-mappings in W-hyperbolic spaces

Throughout this paper, we introduce a class of asymptotically nonexpansive nonself-mapping and modify the classical Kuhfittig iteration algorithm in the general setup of hyperbolic space. Also, △-convergence results are obtained under a limit condition. The results presented in the paper extend various results in the existing literature.

Surface Immobilization of Human Arginase-1 with an Engineered Ice Nucleation Protein Display System in E. coli

Ice nucleation protein (INP) is frequently used as a surface anchor for protein display in gram-negative bacteria. Here, MalE and TorA signal peptides, and three charged polypeptides, 6×Lys, 6×Glu and 6×Asp, were anchored to the N-terminus of truncated INP (InaK-N) to improve its surface display efficiency for human Arginase1 (ARG1). Our results indicated that the TorA signal...

Strong convergence theorems of the Halpern-Mann’s mixed iteration for a totally quasi-ϕ-asymptotically nonexpansive nonself multi-valued mapping in Banach spaces

spaces Liu Hong Bo 0 Li Yi 0 0 School of Science, Southwest University of Science and Technology , Mianyang, Sichuan 621010 , P.R. China In this paper, we introduce a class of totally quasi-φ

Dynamic identifying protein functional modules based on adaptive density modularity in protein-protein interaction networks

Background The identification of protein functional modules would be a great aid in furthering our knowledge of the principles of cellular organization. Most existing algorithms for identifying protein functional modules have a common defect -- once a protein node is assigned to a functional module, there is no chance to move the protein to the other functional modules during the...

Protective effect of HDL on NADPH oxidase-derived super oxide anion mediates hypoxia-induced cardiomyocyte apoptosis

Cardiovascular diseases are the leading cause of death of death in Taiwan. Atherosclerosis can lead to serious problems, including heart attack, stroke, or even death. Coronary heart disease (CHD) occurs when plaque builds up in the coronary arteries to cause the ischemic heart disease which will enhance myocardial remodeling and also induce myocardial hypoxia. High density...

Strong Convergence Theorems for Modifying Halpern Iterations for Quasi--Asymptotically Nonexpansive Multivalued Mapping in Banach Spaces with Applications

University of Science and Technology, Mianyang, Sichuan 621010, China Received 20 August 2012; Accepted 21 November 2012 Academic Editor: Nan-Jing Huang Copyright © 2012 Li Yi. This is an open access

Strong convergence theorems for a countable family of totally quasi-ϕ-asymptotically nonexpansive nonself mappings in Banach spaces with applications

In this paper, we introduce a class of totally quasi-ϕ-asymptotically nonexpansive nonself mappings and study the strong convergence under a limit condition only in the framework of Banach spaces. Meanwhile, our results are applied to study the approximation problem of a solution to a system of equilibrium problems. The results presented in the paper improve and extend the...

Crystal Structure and Identification of Two Key Amino Acids Involved in AI-2 Production and Biofilm Formation in Streptococcus suis LuxS

Streptococcus suis has emerged as an important zoonotic pathogen that causes meningitis, arthritis, septicemia and even sudden death in pigs and humans. Quorum sensing is the signaling network for cell-to-cell communication that bacterial cells can use to monitor their own population density through production and exchange of signal molecules. S-Ribosylhomocysteinase (LuxS) is...

Semaphorin 3a transfection into the left stellate ganglion reduces susceptibility to ventricular arrhythmias after myocardial infarction in rats

Myocardial infarction (MI) induces neural remodelling of the left stellate ganglion (LSG), which may contribute to ischaemia-induced arrhythmias. The neural chemorepellent Semaphorin 3a (Sema3a) has been identified as a negative regulator of sympathetic innervation in the LSG and heart. We previously reported that overexpression of Sema3a in the border zone could reduce the...

Identification and characterization of a Streptococcus equi ssp. zooepidemicus immunogenic GroEL protein involved in biofilm formation

Streptococcus equi ssp. zooepidemicus (S. equi spp. zooepidemicus) is an opportunistic pathogen that causes major economic losses in the swine industry in China and is also a threat for human health. Biofilm formation by this bacterium has been previously reported. In this study, we used an immunoproteomic approach to search for immunogenic proteins expressed by biofilm-grown S...

Role of Myeloma-Derived MIF in Myeloma Cell Adhesion to Bone Marrow and Chemotherapy Response

Background: Multiple myeloma (MM) remains an incurable cancer characterized by accumulation of malignant plasma cells in the bone marrow (BM). The mechanism underlying MM homing to BM is poorly elucidated.

High-level expression of human arginase I in Pichia pastoris and its immobilization on chitosan to produce L-ornithine

Background L-ornithine (L-Orn), is an intermediate metabolite in the urea cycle that plays a significant role in humans. L-Orn can be obtained from the catalysis of L-arginine (L-Arg) by arginase. The Pichia pastoris expression system offers the possibility of generating a large amount of recombinant protein. The immobilized enzyme technology can overcome the difficulties in...

PALB2 mutations in breast cancer patients from a multi-ethnic region in northwest China

Background Germline mutations in PALB2 gene make a small contribution to heritable breast cancer susceptibility. A recent report has revealed that women with mutations in the PALB2 gene were more than nine times as likely to develop breast cancer compared to those without. The aim of this study is to understand the status of PALB2 mutations among Chinese high-risk breast cancer...

Dispersion and aggregation of single-walled carbon nanotubes in aqueous solutions of anionic surfactants

Understanding the dispersion and aggregation of carbon nanotubes (CNTs) in the aqueous environment are critical for the fate, bioavailability, and the environment and health risk assessment of them because the better suspended CNTs display a higher mobility and could transfer to a longer distance in the environment to possibly pose greater ecological and environmental risks. In...

Structural dynamics of the two-component response regulator RstA in recognition of promoter DNA element

The RstA/RstB system is a bacterial two-component regulatory system consisting of the membrane sensor, RstB and its cognate response regulator (RR) RstA. The RstA of Klebsiella pneumoniae (kpRstA) consists of an N-terminal receiver domain (RD, residues 1–119) and a C-terminal DNA-binding domain (DBD, residues 130–236). Phosphorylation of kpRstA induces dimerization, which allows...

Development of a nanoparticle-assisted PCR (nanoPCR) assay for detection of mink enteritis virus (MEV) and genetic characterization of the NS1 gene in four Chinese MEV strains

Background Mink enteritis virus (MEV) causes mink viral enteritis, an acute and highly contagious disease whose symptoms include violent diarrhea, and which is characterized by high morbidity and mortality. Nanoparticle-assisted polymerase chain reaction (nanoPCR) is a recently developed technique for the rapid detection of bacterial and viral DNA. Here we describe a novel...