Advanced search    

Search: authors:"Donald P. McManus"

90 papers found.
Use AND, OR, NOT, +word, -word, "long phrase", (parentheses) to fine-tune your search.

Correlative and Dynamic Imaging of the Hatching Biology of Schistosoma japonicum from Eggs Prepared by High Pressure Freezing

Background Schistosome eggs must traverse tissues of the intestine or bladder to escape the human host and further the life cycle. Escape from host tissues is facilitated by secretion of immuno-reactive molecules by eggs and the formation of an intense strong granulomatous response by the host which acts to exclude the egg into gut or bladder lumens. Schistosome eggs hatch on...

Recombinant antigens for immunodiagnosis of cystic echinococcosis

Three cDNAs, termed EpC1, TPxEg and EgG5, were isolated by immunoscreening from an Echinococcus granulosus cDNA library. The recombinant phages exhibited strong reactivity with sera from humans with confirmed cystic echinococcosis (CE) and with sera from mice infected with E. granulosus oncospheres. The cDNAs were subcloned into a pET vector, expressed as fusion proteins tagged...

CD4+ T-CELL COUNTS, CD4+/CD8+ T-CELL COUNT RATIOS, AND ANTIBODY LEVELS IN MIGRANT FISHERMEN INFECTED WITH SCHISTOSOMA JAPONICUM IN THE DONGTING LAKE, CHINA

In this study, CD4+ and CD8+ T cells and antibody levels were measured in 94 migrant fishermen infected with Schistosoma japonicum from Dongting Lake, China. Prevalence among these fishermen was high (63.8%), with a mean infection intensity of 61.4 ± 3.8 epg, and included a high proportion of individuals (39.4%) with substantial parenchymal fibrosis (stages ≥ 2/3). The CD4+/CD8...

Artemether Treatment of Prepatent Schistosoma japonicum Induces Resistance to Reinfection in Association with Reduced Pathology

Artemether (ART) is a well-described antimalarial with efficacy against juvenile schistosomes, with 7-day-old schistosomula being particularly susceptible. Both ART-affected worms and parasites developing from irradiated cercariae die at similar times after infection. Our aim was to determine if ART treatment of prepatent schistosomiasis japonica may result in the generation of a...

Cloning and Characterisation of Schistosoma japonicum Insulin Receptors

Background Schistosomes depend for growth and development on host hormonal signals, which may include the insulin signalling pathway. We cloned and assessed the function of two insulin receptors from Schistosoma japonicum in order to shed light on their role in schistosome biology. Methodology/Principal Findings We isolated, from S. japonicum, insulin receptors 1 (SjIR-1) and 2...

A Pilot Study for Control of Hyperendemic Cystic Hydatid Disease in China

Background Cystic hydatid disease (CHD) is a global parasitic zoonosis caused by the dog tapeworm, Echinococcus granulosus. The disease is hyperendemic in western China because of poor economic development; limited community knowledge of CHD; widespread, small-scale household animal production; home killing of livestock; and the feeding of dogs with uncooked offal. Methodology...

Natural Infection of the Ground Squirrel (Spermophilus spp.) with Echinococcus granulosus in China

Donald P. McManus Hector H. Garcia, Universidad Peruana Cayetano Heredia, Peru Background: Echinococcus granulosus is usually transmitted between canid definitive hosts and ungulate intermediate hosts

Comparison of two approaches for measuring household wealth via an asset-based index in rural and peri-urban settings of Hunan province, China

McManus 0 Yue-Sheng Li 0 Zheng-Yuan Zhao Li-Ping Yuan Jürg Utzinger Gail M Williams Ying Li Mao-Yuan Ren Zong-Chuan Liu Jie Zhou Giovanna Raso 0 0 Queensland Institute of Medical Research, P.O. Royal

A Novel Procedure for Precise Quantification of Schistosoma japonicum Eggs in Bovine Feces

Schistosomiasis japonica is a zoonosis with a number of mammalian species acting as reservoir hosts, including water buffaloes which can contribute up to 75% to human transmission in the People's Republic of China. Determining prevalence and intensity of Schistosoma japonicum in mammalian hosts is important for calculating transmission rates and determining environmental...

A Novel Recombinant Antigen for Immunodiagnosis of Human Cystic Echinococcosis

A pool of serum samples from mice infected with oncospheres (eggs) of Echinococcus granulosus was used to screen a cDNA library constructed with RNA extracted from protoscolex larvae from sheep hydatid cysts. One immunoreactive clone, designated EpC1 was shown to encode a protein of 76 residues. The complementary DNA (cDNA) fragment was subcloned into an expression vector, pET...

A 5-year longitudinal study of schistosomiasis transmission in Shian village, the Anning River Valley, Sichuan Province, the Peoples' Republic of China

Wellcome Trust (WT) and the National Health and Medical Research Council (NHMRC), Australia. Donald P McManus is a NHMRC Senior Principal Research Fellow; Yuesheng Li is an Australian Research Council Future

Landscape Composition and Spatial Prediction of Alveolar Echinococcosis in Southern Ningxia, China

Background Alveolar echinococcosis (AE) presents a serious public health challenge within China. Mass screening ultrasound surveys can detect pre-symptomatic AE, but targeting areas identified from hospital records is inefficient regarding AE. Prediction of undetected or emerging hotspots would increase detection rates. Voles and lemmings of the subfamily Arvicolinae are...

Five-Year Longitudinal Assessment of the Downstream Impact on Schistosomiasis Transmission following Closure of the Three Gorges Dam

. McManus 0 Rachel L. Pullan, London School of Hygiene and Tropical Medicine, United Kingdom 0 1 Molecular Parasitology Laboratory, Infectious Diseases Division, Queensland Institute of Medical Research

A Cluster-Randomised Intervention Trial against Schistosoma japonicum in the Peoples' Republic of China: Bovine and Human Transmission

Background Zoonotic schistosomiasis japonica is a major public health problem in China. Bovines, particularly water buffaloes, are thought to play a major role in the transmission of schistosomiasis to humans in China. Preliminary results (1998–2003) of a praziquantel (PZQ)-based pilot intervention study we undertook provided proof of principle that water buffaloes are major...

ANTIGEN-SPECIFIC ANTIBODY ISOTYPE PATTERNS TO SCHISTOSOMA JAPONICUM RECOMBINANT AND NATIVE ANTIGENS IN A DEFINED POPULATION IN LEYTE, THE PHILIPPINES

We describe antibody isotype patterns resulting from Schistosoma japonicum infection among 155 individuals 5–76 years old from a community in Leyte, The Philippines. Their exposure, infection, and reinfection status had been observed in a previously described water contact study used to categorically classify them as putative resistant or susceptible individuals. Antigens tested...

Zoonoses and marginalised infectious diseases of poverty: Where do we stand?

Despite growing awareness of the importance of controlling neglected tropical diseases as a contribution to poverty alleviation and achieving the Millennium Development Goals, there is a need to up-scale programmes to achieve wider public health benefits. This implementation deficit is attributable to several factors but one often overlooked is the specific difficulty in tackling...

A Cluster-Randomized Bovine Intervention Trial against Schistosoma japonicum in the People’s Republic of China: Design and Baseline Results

Prevention, 207 Rui Jin Er Lu, Shanghai 200005, Peoples Republic of China. Donald P. McManus, Australian Centre for International and Tropical Health and Nutrition, The University of Queensland and The

SHORT REPORT: ECHINOCOCCUS GRANULOSUS FROM XINJIANG, PR CHINA: cDNAS ENCODING THE EG95 VACCINE ANTIGEN ARE EXPRESSED IN DIFFERENT LIFE CYCLE STAGES AND ARE CONSERVED IN THE ONCOSPHERE

, Urumqi, Xinjiang, China 1 Authors' addresses: Jun Li, Hong You, Alex Loukas, and Donald P. McManus. Molecular Parasitology Laboratory, Australian Centre for International and Tropical Health and Nutrition

Regulatory Role of Interleukin-10 and Interferon-γ in Severe Hepatic Central and Peripheral Fibrosis in Humans Infected with Schistosoma japonicum

Background. Schistosoma japonicum is the most pathogenic agent of hepatosplenic schistosomiasis. It causes fibrosis of the central (CentF) and peripheral (PerF) portal areas. We investigated whether CentF and PerF in Chinese fishermen infected with S. japonicum were associated with an abnormal production of cytokines and chemokines that, in experimental models, have been...