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Direct imaging of the circular chromosome in a live bacterium

genomic insertions of gene cassettes. To obtain circular chromosomes, we grew cells in liquid M9 minimum medium (Fluka Analytical) supplemented with 2 mM MgSO4, 0.1 mM CaCl2, 0.4% glycerol (Sigma?Aldrich ... ), and 0.01% protein hydrolysate amicase (PHA) (Fluka Analytical) overnight at 30 ?C to reach late exponential phase. We then pipetted 1 ?l culture onto a cover glass and immediately covered the cells47

Critical fluctuations and slowing down of chaos

singular state. Numerical simulations and analytical models show how the ordering mechanisms of critical dynamics are measurable through the hierarchy of spatiotemporal Lyapunov vectors. A subset of unstable ... critical temperature55. Here, by treating the nonlinear dynamics directly with numerical simulations and analytical models, we have resolved the phase space directions responsible for the thermodynamic

Direct observation of atomic-scale fracture path within ceramic grain boundary core

case may be therefore selected as the preferable fracture path of the grain boundary. These results indicate that the local atomic coordination chemistry of the segregated cation in grain boundary core

The ecological drivers of variation in global language diversity

use similar data to Axelson & Manrubia13, there are a number of differences in our analytical approach. To compare our results to theirs, we reanalyze our data using their method, fitting continent

Side chain to main chain hydrogen bonds stabilize a polyglutamine helix in a transcription factor

of Barcelona (CCiTUB) , Baldiri Reixac 10, 08028 Barcelona , Spain 5 CERM and Department of Chemistry ?Ugo Schiff?, University of Florence , Via Luigi Sacconi 6, Sesto Fiorentino, 50019 Florence ... , Italy 6 Department of Biochemistry and Biomedicine, University of Barcelona , Avinguda Diagonal 645, 08028 Barcelona , Spain 7 Institute for Advanced Chemistry of Catalonia (IQAC-CSIC) , Jordi Girona 18

Author Correction: Lattice strain-enhanced exsolution of nanoparticles in thin films

Department of Materials Science and Engineering, Kumoh National Institute of Technology , Gumi 39177 , Republic of Korea 4 1Fcell Inc. , Pohang 37673 , Republic of Korea 5 School of Chemistry, University of St

Structural basis for reversible amyloids of hnRNPA1 elucidates their role in stress granule assembly

, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences , Shanghai 201210 , China 1 University of Chinese Academy of Sciences , Beijing 100049 , China 2 Bio-X Institutes, Key Laboratory for the

Lipopolysaccharide inhalation recruits monocytes and dendritic cell subsets to the alveolar airspace

analytical flow cytometry and cell sorting. 331506; 1:40), anti-CD11b-APC (ICRF44; Biolegend; 301309; 1:25), anti-BTLA-PE (J168?540; BD; 558485; 1:100), anti-Axl-APC (108724; R&D Systems; FAB154A: 1:33) and ... -FcER1-PE (CRA1; eBioscience; 12?5899?42; 1:25) and anti-CD86-PE (FUN-1; BD; 555658; 1:25). Dead cells were excluded with DAPI (Partec). Analytical flow cytometry was performed on a BD Fortessa X20 or BD

Dynamics of pore formation during laser powder bed fusion additive manufacturing

pore formation at laser turn points by modulating the laser power to compensate for melt pool overheating. An analytical model defining the dimensionless quantity normalized enthalpy18,34 as a function ... turn points per volume slice is increased significantly42. An analytical approach, utilizing normalized enthalpy18,34,43, was used to investigate the outcomes of the mitigation strategy. Normalized

A tightly-bonded and flexible mesoporous zeolite-cotton hybrid hemostat

. The effect of the zeolite clinoptilolite on serum chemistry and hematopoiesis in mice . Food Chem. Toxicol . 39 , 717 - 727 ( 2001 ). 13. Tarbuk , A. , Grancaric , A. M. & Magas , S. Modified cotton

Regioselective radical α-borylation of α,β-unsaturated carbonyl compounds for direct synthesis of α-borylcarbonyl molecules

Organoboron compounds are highly valuable in synthetic chemistry. In particular, α-borylcarbonyl compounds have shown versatile synthetic applications, owing to fruitful chemistries of both the boryl ... advantages in diverse and economical synthesis of valuable ?-borylcarbonyl molecules. - O in chemical synthesis, medicinal chemistry, and material rganoboron compounds have been substantially applied

Quantifying the factors limiting rate performance in battery electrodes

widely used for fitting purposes. Alternatively, a number of fittable, analytical, physical models are available but only describe the high-rate region24,29. Due to these limitations, a number of empirical

Mendelian randomization analysis using mixture models for robust and efficient estimation of causal effects

and efficiency gain compared to MR-mode (Supplementary Figures 6?9). Simulation studies also showed that when the number of selected instruments were large, the analytical formula of standard error

High-resolution specificity profiling and off-target prediction for site-specific DNA recombinases

Chas Leichner 4 Paul A. Clemons David R. Liu 3 0 Merkin Institute of Transformative Technologies in Healthcare, Broad Institute of Harvard and MIT , Cambridge, MA 02142 , USA 1 Department of Chemistry

Coupling carbon and energy fluxes in the North Pacific Subtropical Gyre

were determined using an Exeter Analytical Elemental Analyzer using a combustion temperature of 1020 ?C. Acetanilide was used as a standard and the Hawaii Ocean Time-series (HOT) plankton sample was used ... the CTO-375 method of Gustafsson et al.44. A recent laboratory intercomparison of BC quantification in 12 different source materials using 7 different analytical methods45 provides a comprehensive

Binary tree-inspired digital dendrimer

Chemistry, Chemical Engineering and Materials Science, Soochow University , 215123 Suzhou , China 1 Computer Science and Artificial Intelligence Laboratory, Massachusetts Institute of Technology , Cambridge ... , MA 02139 , USA 2 Department of Chemistry, University of South Florida , Tampa , USA Digital polymers with precisely ordered units acting as the coded 0- or 1-bit, are introduced as a promising option

A chemical toolbox for the study of bromodomains and epigenetic signaling

, Germany 4 Institute of Pharmaceutical Chemistry, Goethe-University Frankfurt , 60438 Frankfurt , Germany 5 Sprott Centre for Stem Cell Research, Regenerative Medicine Program, Ottawa Hospital Research ... . However, as an analytical tool the Tm shift assay provides a good platform for assessment of selectivity when hits are carefully followed up using orthogonal binding assays. Some probe compounds were

RNA G-quadruplex is resolved by repetitive and ATP-dependent mechanism of DHX36

Biophysics, Johns Hopkins University , Baltimore, MD 21218 , USA 1 Department of Chemistry, University of Cambridge , Cambridge CB2 1TN , UK 2 Biochemistry and Biophysics Center, National Heart, Lung and Blood

A 3.8 Å resolution cryo-EM structure of a small protein bound to an imaging scaffold

due to poorly understood surface chemistry and mechanical forces30,31. Scaffold approaches should also ultimately provide advantages in streamlined cryo-EM data processing, as a large symmetric core

Phase-field modeling and machine learning of electric-thermal-mechanical breakdown of polymer-based dielectrics

different properties. Machine learning is conducted on the database from the high-throughput simulations to produce an analytical expression for the breakdown strength, which is verified by targeted ... corresponding breakdown strengths of the P(VDF-HFP)-based nanocomposites. Then, machine learning is performed on the high-throughput simulation results to produce an analytical expression for the breakdown