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Gel-eletroforese no diagnóstico da varíola

The test of gel-electrophoresis applied to the pox virus group showed to be at least thirth times (30X) more sensitive than agar-gel test on the described conditions (Table I). Twelve specimens, which were positives form Smallpox in the conventional tests of egg inoculation and agar-gel difusion test, have been screened in their original dilutions frozen for more than 1 year and...

Subdoses of 17DD yellow fever vaccine elicit equivalent virological/immunological kinetics timeline

Villanova Freire Edson Pereira Filho Maria da Luz Fernandes Leal Akira Homma Andra Teixeira-Carvalho Reinaldo Menezes Martins Olindo Assis Martins-Filho Background: The live attenuated 17DD Yellow Fever

17DD and 17D-213/77 Yellow Fever Substrains Trigger a Balanced Cytokine Profile in Primary Vaccinated Children

Background This study aimed to compare the cytokine-mediated immune response in children submitted to primary vaccination with the YF-17D-213/77 or YF-17DD yellow fever (YF) substrains. Methods A non-probabilistic sample of eighty healthy primary vaccinated (PV) children was selected on the basis of their previously known humoral immune response to the YF vaccines. The selected...

Cytokine Signatures of Innate and Adaptive Immunity in 17DD Yellow Fever Vaccinated Children and Its Association With the Level of Neutralizing Antibody

Background. The live attenuated yellow fever (YF) vaccines have been available for decades and are considered highly effective and one of the safest vaccines worldwide. Methods. The impact of YF-17DD-antigens recall on cytokine profiles of YF-17DD-vaccinated children were characterized using short-term cultures of whole blood samples and single-cell flow cytometry. This study...

Invasive Streptococcus pneumoniae Infection in Latin American Children: Results of the Pan American Health Organization Surveillance Study

Protein-polysaccharide conjugate vaccines against Streptococcus pneumoniae promise to be an effective public health intervention for children, especially in an era of increasing antimicrobial resistance. To characterize the distribution of capsular types in Latin America, surveillance for invasive pneumococcal infection in children ⩽5 years of age was done in six countries...

Levantamento epidemiológico e virológico de casos suspeitos de poliomielite no Hospital Estadual Jesus, no período de janeiro de 1967 a março de 1968

The authors present results obtained by the inoculation in tissue culture of material frorn rectal swabs of susyected paralitic Polio cases, at the Hospital Jesus, at Rio de Janeiro. Among 188 swabs that have been examinated, Poliovírus could be demonstrated in 79 cases (73,5% were positive for type I, 25,3% for type II and 1,2% for type III). An increase of paralitic Polio cases...